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Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application

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Journal of Oceanology and Limnology 2021年第6期39卷 2107-2115页

作者： Changyou CHEN Pingping WANG long-fei wu Tao SONG Beijing Key Laboratory of Bioelectromagnetism Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms Chinese Academy of SciencesBeijing 100029China Aix Marseille University CNRSLCBMarseille F-13402France University of the Chinese Academy of Sciences Beijing 100049China

Magnetotactic bacteria are capable of biosynthesizing magnetic nanoparticles,also called magnetosomes,and swimming along magnetic field *** abilities endow the whole cells of magnetotactic bacteria with such applications as targeted therapy and manipulation of *** have shown that the intact marine magnetotactic bacteria MO-1 kill efficiently antibiotic-resistant pathogen Staphylococcus aureus in vivo,but the biocompatibility of this marine bacterium is *** this study,the strain MO-1 was chosen to analyze its biocompatibility and potential for biomedicine *** showed that MO-1 cells could be guided at 37℃ under an external magnetic field and swim in the blood plasma and *** could keep active locomotivity within 40 min in the plasma and urine,although their velocity slowed *** incubated with human cells,magnetotactic bacteria MO-1 had no obvious effects on cellular viability at low dose,while the cell toxicity increased with the augmentation of the quantity of the MO-1 cells *** the in-vivo experiments,the median lethal dose of magnetotactic bacteria MO-1 in rats was determined to be 7.9×10^(10) bacteria/*** results provided the foundation for the biocompatibility and safety evaluations of magnetotactic bacteria MO-1 and suggested that they could be basically used in clinical targeted therapy.

关键词： magnetotactic bacteria motility cell interaction median lethal dose

Morphological and phylogenetic diversity of magnetotactic bacteria and multicellular magnetotactic prokaryotes from a mangrove ecosystem in the Sanya River,South China

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Journal of Oceanology and Limnology 2021年第6期39卷 2015-2026页

作者： Haitao CHEN Jinhua LI long-fei wu Wei-Jia ZHANG Beijing Key Laboratory of Biological Electromagnetism Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms Chinese Academy of SciencesBeijing 100029China Deep-Sea Microbial Cell Biology Department of Deep Sea SciencesInstitute of Deep-Sea Science and EngineeringChinese Academy of SciencesSanya 572000China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China LCB Aix Marseille UniversityCNRSMarseille 13402France

Magnetotactic bacteria(MTB)are morphologically and phylogenetically diverse prokaryotes commonly able to produce magnetic nanocrystals within intracellular membrane-bound organelles(i.e.,magnetosomes)and to swim along geomagnetic field *** studied the diversity of MTB in the samples collected from a mangrove area in the Sanya River,Hainan,South China,using microscopic and microbial phylogenetic *** of microanalysis and observation in microscopy and energy dispersive X-ray spectroscopy(EDXS)reveal a highly morphological diversity of MTB including unicellular cocci,vibrios,rod-shaped bacteria,and three morphotypes of multicellular magnetotactic prokaryotes(MMPs).In addition,analysis of the 16S rRNA gene showed that these MTB were clustered into 16 operational taxonomic units affi liated to the Alpha-,Delta-,and Gamma-proteobacteria classes within the Proteobacteria ***,by using the coupled fluorescence and transmission electron microscopy analysis,rodshaped bacteria,vibrio,and cocci were phylogenetically and structurally identified at the single-cell *** study demonstrated highly diverse MTB communities in the mangrove ecosystem and provide a new insight into the overall diversity of MTB.

关键词： magnetotactic bacteria diversity magnetosome phylogenetic analysis coordinated fl uorescence in-situ hybridization transmission electron microscopy(FISH-TEM) mangrove

Genomic analysis of a pure culture of magnetotactic bacterium Terasakiella ***-1

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Journal of Oceanology and Limnology 2021年第6期39卷 2097-2106页

作者： Haijian DU Wenyan ZHANG Wei LIN Hongmiao PAN Tian XIAO long-fei wu College of Life Science Shandong UniversityQingdao 266237China CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Institutions of Earth Science Chinese Academy of SciencesBeijing 100029China France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms Chinese Academy of SciencesBeijing 100029China Aix-Marseille University CNRSLCBMarseille F-13402France

Magnetotactic bacteria(MTB)display magnetotaxis ability because of biomineralization of intracellular nanometer-sized,membrane-bound organelles termed *** having been discovered more than half a century,only a few representatives of MTB have been isolated and cultured in the *** this study,we report the genomic characterization of a novel marine magnetotactic spirillum strain SH-1 belonging to the genus Terasakiella that was recently isolated.A gene encoding haloalkane dehalogenase,which is involved in the degradation of chlorocyclohexane,chlorobenzene,chloroalkane,and chloroalkene,was ***-1 genome contained cysCHI and soxBAZYX genes,thus potentially capable of assimilatory sulfate reduction to H_(2)S and using thiosulfate as electron donors and oxidizing it to *** of SH-1 also contained genes encoding periplasmic dissimilatory nitrate reductases(napAB),assimilatory nitrate reductase(nasA)and assimilatory nitrite reductases(nasB),suggesting that it is capable of gaining energy by converting nitrate to *** pure culture of Terasakiella ***-1 together with its genomic results off ers new opportunities to examine biology,physiology,and biomineralization mechanisms of MTB.

关键词： magnetotactic bacteria magnetotaxis pure culture comparative genomic analysis

Assessing bacterial magnetotactic behavior by using permanent magnet blocks

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Chinese Science Bulletin 2014年第17期59卷 1929-1935页

作者： Tao Song Hong-Miao Pan Zheng Wang Tian Xiao long-fei wu Beijing Key Laboratory of Bioelectromagnetism Institute of Electrical EngineeringChinese Academy of Sciences France-China Bio-Mineralization and Nano-Structures Laboratory Key Laboratory of Marine Ecology & Environmental Sciences Institute of OceanologyChinese Academy of Sciences Laboratory of Bacterial Chemistry UMR7283Institute of Mediterranean MicrobiologyAix-Marseille UniversityCNRSF-13402 Marseille Cedex 20France

Assessing the movement of magnetotactic bacteria(MTB)under magnetic fields is a key to exploring the function of the *** this study,a simple method was used to analyze the behavior of MTB,which was based on the accumulation of cells on the walls of a test tube when two permanent magnet blocks were applied on the *** results showed a significant difference among the movements of the polar MTB,axial MTB,and *** polar magnetotactic cells aggregated as spots above or below the two magnet blocks besides the aggregated spots underneath the magnet *** contrast,the axial magnetotactic cells aggregated only as two round spots underneath the magnet blocks,and more cells aggregated in the center than all around of the *** the ferrofluid,two spots were also formed underneath the magnet blocks,and the aggregated particles formed a ring *** calculation by finite element method was used to analyze the phenomenon,and the findings were reasonably explained by the MTB features and magnetic field theory.A scheme that differentiates polar MTB,axial MTB,and magnetic impurity could be developed,which would be beneficial to fieldworks involving MTB in the future.

关键词：趋磁细菌铁块行为评估永久细胞聚集磁场计算铁磁流体

Observations on a magnetotactic bacteria-grazing ciliate in sediment from the intertidal zone of Huiquan Bay,China

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Journal of Oceanology and Limnology 2021年第6期39卷 2053-2062页

作者： Si CHEN Kaixuan CUI Wenyan ZHANG Yicong ZHAO Tian XIAO Hongmiao PAN wuchang ZHANG long-fei wu CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France

Magnetotactic bacteria(MTB)are a group of prokaryotes having the ability to orient and swim along geomagnetic field lines because they contain intracellular magnetosomes that are synthesized through a biomineralization *** have recently also been found in unicellular eukaryotes,which are referred to as magnetically responsive protists(MRPs).The magnetosomes have three origins in *** this study,we characterized a MTB-grazing ciliated MRP that was magnetically collected from intertidal sediment of Huiquan Bay,Qingdao,*** on 18S rRNA gene sequence analysis,the ciliated MRP was tentatively identified as Uronemella parafi lificum *** transmission electron microscopy,we observed that magnetosomes having 2-3 shapes were randomly distributed within this *** X-ray spectroscopy and high-resolution transmission electron microscopy images of the magnetosomes were consistent with them being composed of *** having the same shape and mineral composition were also detected in MTB that occurred in the same environment as the ciliated *** analysis showed that the size and shape of the magnetosomes in the ciliated MRP were similar to those in *** results suggest that this ciliated MRP can graze,ingest,and digest various types of *** is certainly worth noting that this is the first record of MRPs in Asian aquatic sediment and suggesting they might be widely *** results also support the assertion that MRPs probably contribute to the ecological cycles of iron,and expand possibilities for research into the mechanism of magnetoreception in eukaryotes.

关键词： magnetically responsive protist ciliate magnetotactic bacteria magnetosome graze magnetoreception

Characterization and diversity of magnetotactic bacteria from sediments of Caroline Seamount in the Western Pacific Ocean

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Journal of Oceanology and Limnology 2021年第6期39卷 2027-2043页

作者： Kaixuan CUI Wenyan ZHANG Jia LIU Cong XU Yicong ZHAO Si CHEN Hongmiao PAN Tian XIAO long-fei wu CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France National Oceanographic Center Qingdao 266071China

Magnetotactic bacteria(MTB)are a group of microorganisms capable of orientating and swimming along magnetic fields because they contain intracellular biomineralized magnetosomes composed of magnetite(Fe 3 O 4)or/and greigite(Fe_(3)S_(4)).They are ubiquitous in freshwater,brackish,and marine habitats,and are cosmopolitan in ***,knowledge of their occurrence and distribution in seamount ecosystems is *** investigated the diversity and distribution of MTB in the Caroline Seamount(CM4).The abundance of living MTB in 12 stations in depth varying from 90 to 1545 m was 1.1×10^(3)-43.7×10^(3) inds./dm *** diverse shapes of MTB observed,magnetotactic cocci were the dominant morphotype and could be categorized into two types:1)typical cocci that appeared to have peritrichous fl agella;and 2)those characterized by having a drop-shaped form and one bundle of fl agella located at the thin/narrow end of the *** electron microscopy(TEM)analysis revealed that the magnetosomes formed by those magnetotactic cocci are magnetite(Fe 3 O 4)with octahedral crystal habit.A total of 41 operational taxonomic units(OTUs)of putative MTB(2702 reads)were acquired from nine stations,based on high-throughput *** these,40 OTUs belonged to the Proteobacteria phylum and one belonged to the Nitrospirae *** found apparent connectivity between the MTB populations on the Caroline and Kexue(Science in Chinese)seamounts,although the diversity of MTB on Caroline was much richer than on the Kexue *** results imply that the unique topography of seamounts and other as-yet unclear environmental factors could lead to evolution of different fl agella arrangements in magnetotactic cocci,and the occurrence of octahedral magnetite magnetosomes.

关键词： magnetotactic bacteria Caroline Seamount abundance diversity magnetosome

Synthesis of α-Aminoarylacetic Acids in the Presence of Phase Transfer Catalysts

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Chinese Chemical Letters 1999年第10期10卷 813-816页

作者： Wei Liang XU You Fang CHEN fei long wu(Basic Course Division, Zhejiang Agricultural University, Hangzhou 310029) Basic Course Division Zhejiang Agricultural University Hangzhou 310029 China

Synthesis of aminoarylacetic acids with good yield in the presence of phase transfercatalyst (PTC) under sonication has been studied. Results obtained are superior to those by othersimilar methods. The data show that cyclodextrin (5 -CD) is a more efficient catalyst thanother PTC. Optimum reaction conditions and a preliminary mechanism are also discussed.

关键词： α-Aminoarylacetic acid phase transfer catalyst sonication.

How light affect the magnetotactic behavior and reproduction of ellipsoidal multicellular magnetoglobules?

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Journal of Oceanology and Limnology 2021年第6期39卷 2005-2014页

作者： Xinxin QIAN Yicong ZHAO Claire-Lise SANTINI Hongmiao PAN Tian XIAO Haitao CHEN Tao SONG Jinhua LI Francois ALBERTO Sophie BRUSTLEIN long-fei wu State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Aix Marseille University CNRSLCBCenturiIM2BIMMMarseille 13009France International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASMarseille 13402France CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Institute of Electrical Engineering Chinese Academy of SciencesBeijing 100190China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Aix Marseille University CenturiMarseille 13009France

Magnetotactic bacteria(MTB)synthesize intracellular magnetic organelles,magnetosomes,which consist of magnetic crystals that are enveloped in a *** are organized into a chain(s)and confer on cells a magnetic dipolar *** magnetic property allows MTB cells to align and swim along geomagnetic field lines,a movement referred to as *** MTB species change their swim direction in response to illumination by UV,violet and blue *** we analyzed the polarity of morphology,magnetism,and motion in Mediterranean multicellular magnetotactic prokaryotes,also called,magnetoglobules or *** magnetoglobules were assembled from 60-80 cells into an asymmetric ellipsoidal morphology with a relative narrow and large *** swam dominantly northward,parallel to the direction of the magnetic field,with the narrow-end as the leading *** response to a reversal in the direction of the magnetic field,they aligned quickly along the magnetic field lines and kept swimming ***,under constant illumination,385-nm UV light,magnetoglobules changed their swimming direction southward anti-parallel to the direction of the magnetic field,with the large-end as the leading *** change from a northward to southward direction occurred along with an increase of swimming speed.A minimum of 35-mW/cm^(2) irradiance of UV light was sufficient to trigger the swimming *** radiation also triggered the unidirectional division of *** these results revealed a coordination of the polarity of magnetoglobule morphology,magnetic moment,and swimming orientation,in response to magnetic and optical *** UV triggered the reversal of magnetotaxis and magnetoglobule division indicating the ecological significance of light for multicellular magnetotactic prokaryotes.

关键词： photo-response magnetic alignment coordinated swimming

Lignin-reinforced PVDF electrolyte for dendrite-free quasi-solid-state Li metal battery

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Rare Metals 2024年第3期43卷 1006-1016页

作者： Xin-Yang Chen Xue-Jie Gao Han-Yan wu Yu-long Liu Xiao-fei Yang Run-Cang Sun Center for Lignocellulosic Chemistry and Biomaterials College of Light Industry and Chemical EngineeringDalian Polytechnic UniversityDalian116034China Division of Energy Storage Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China School of Chemistry Northeast Normal UniversityChangchun130024China

Quasi-solid-state lithium metal batteries(QSSLMBs)assembled with polyvinylidene fluoride(PVDF)are a promising class of next-generation rechargeable batteries due to their safety,high energy density,and superior interfacial ***,PVDF has a series of inherent drawbacks such as low ionic conductivity,ease of crystallization,and hydrophobic character that leading to poor cell *** tackle these issues,a lignin-reinforced PVDF electrolyte is proposed in this work to solve these drawbacks of PVDF and enhance the comprehensive performance of *** lithophilic polar groups of lignin can promote uniform deposition of Li on the *** with the improved mechanical properties can efficiently prevent Li dendrites penetration through the *** addition,more active sites provided by lignin can also enhance Li^(+)transport and lead to a faster electrochemical reaction *** from the ingenious design,Li symmetric cells with 5%lignin-PVDF quasi-solid-state electrolyte can operate for 900 h at a high current density/capacity of 5 mA·cm^(-2)/5 mAh·cm^(-2),while shortcircuiting occurs after 56 h for the counterpart(pure PVDF).Moreover,a full cell of Li/5%lignin-PVDF/LFP cell demonstrates a high capacity of 96.2 mAh·g^(-1)after 2000 cycles at 10 *** work is expected to open up promising opportunities to develop other high-energy/power-density QSSLMBs.

关键词： Quasi-solid-state electrolyte Lignin PVDF Dendrite-free Li metal battery