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Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang Baofeng Su De Xing Timothy J.Bruce Shangjia Li logan bern Mei Shang Andrew Johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi Cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

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Enhancing XRF sensor-based sorting of porphyritic copper ore using particle swarm optimization-support vector machine(PSO-SVM)algorithm

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International Journal of Mining Science and Technology 2024年第4期34卷 545-556页

作者： Zhengyu Liu Jue Kou Zengxin Yan Peilong Wang Chang Liu Chunbao Sun Anlin Shao bern Klein School of Civil and Resources Engineering University of Science and TechnologyBeijing 100083China Institute of Minerals Research University of Science and TechnologyBeijing 100083China State Key Laboratory of Mineral Processing Beijing 102628China Ansteel Group Mining Co.Ltd. Anshan 114000China Norman B.Keevil Institute of Mining Engineering University of British ColumbiaVancouverBC V6T 1Z4Canada

X-ray fluorescence(XRF)sensor-based ore sorting enables efficient beneficiation of heterogeneous ores,while intraparticle heterogeneity can cause significant grade detection errors,leading to misclassifications and hindering widespread technology *** classification models are crucial to determine if actual grade exceeds the sorting threshold using localized XRF *** studies mainly used linear regression(LR)algorithms including simple linear regression(SLR),multivariable linear regression(MLR),and multivariable linear regression with interaction(MLRI)but often fell short attaining satisfactory *** study employed the particle swarm optimization support vector machine(PSO-SVM)algorithm for sorting porphyritic copper ore ***-scale results showed PSO-SVM out-performed LR and raw data(RD)models and the significant interaction effects among input features was *** poor input data quality,PSO-SVM demonstrated exceptional ***-scale sorting achieved 93.0%accuracy,0.24%grade increase,84.94%recovery rate,57.02%discard rate,and a remarkable 39.62 yuan/t net smelter return(NSR)increase compared to no *** improvements were achieved by the PSO-SVM model with optimized input combinations and highest data quality(T=10,T is XRF testing times).The unsuitability of LR methods for XRF sensor-based sorting of investigated sample is *** element selection and mineral association analysis elucidate element importance and influence mechanisms.

关键词： XRF sensor-based sorting PSO-SVM algorithm Copper ore pebble Receiver operating curve(ROC) Net smelter return(NSR)

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Galvanic Skin Response—Extinction Biofeedback Training for Psychogenic Abdominal Pain: A Validation Protocol

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Journal of Biosciences and Medicines 2024年第5期12卷 190-201页

作者： Maddalena Castelletti Enrico berné Erasmo Dionigio Carlo Castagnoli Alberto Montagna Giorgio Tonon Magenta Medical Center Milan Italy Castagnoli Medical Center Garlasco Italy GEA Soluzioni Turin Italy

Abdominal and pelvic pain of psychogenic origin is a widespread, disabling, difficult to identify, and often inadequately treated medical condition. This condition is often associated with poor quality of life due to high pain interference with daily activities. Cognitive behavioral psychological therapy and neuromodulation with biofeedback are validated therapies for the treatment of this condition. Aim of the present research work is the validation of a therapeutic protocol that involves the use of both techniques in combination. 20 patients diagnosed with psychogenic abdominal pain, of both sexes, aged between 18 and 60 years who had not benefited from pharmacological therapies were enrolled. 10 patients were randomly assigned to the control group (psychological treatment only), another 10 patients were assigned to the study group (neuromodulation with biofeedback-Galvanic skin response-extinction in combination with psychological therapy). For both groups, the pain score, interference of pain with daily living activities, pain relief, and the share of anxiety associated with the pain condition were evaluated (pre- and post-treatment). The patients who underwent the combined treatment achieved statistically significant better scores than patients in the control group, respectively −4.9 ± 0.9 vs −1.0 ± 0.4 for Pain;−5.1 ± 1.1 vs −0.9 ± 0.3 for Interference with life;−7.2 ± 3.7 vs −2.2 ± 2.1 for HAMA;4.6 ± 1.2 vs 1.1 ± 0.6 for Relief.

关键词： Chronic Abdominal and Pelvic Psychogenic Pain (PAP) Biofeedback Training Pain Management Galvanic Skin Response (GSR)

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Zentrum Paul Klee, bern=伯尔尼保罗·克利博物馆/

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2005年

作者： Zentrum Paul Klee bern

Title translated: 瑞士伯尔尼保罗·克利中心423 p. : ill. (some col.), ports. ; 27 cm. + 1 CD-ROM.

ISBN: (纸本)9783775715331

Title translated: 瑞士伯尔尼保罗·克利中心423 p. : ill. (some col.), ports. ; 27 cm. + 1 CD-ROM.

关键词： Klee Paul 18791940. Catalogs. Painting Swiss 20th century Catalogs. Art museums Switzerland bern.

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Use of centrifugal-gravity concentration for rejection of talc and recovery improvement in base-metal flotation

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International Journal of Minerals,Metallurgy and Materials 2016年第8期23卷 859-867页

作者： bern Klein Naci Emre Altun Hassan Ghaffari NBK Institute of Mining Engineering The University of British Columbia Department of Mining Engineering Middle East Technical University Tetra Tech Metallurgy

The possibility of using a centrifugal-gravity concentrator to reject Mg-bearing minerals and minimize metal losses in the flotation of base metals was evaluated. Sample characterization, batch scoping tests, pilot-scale tests, and regrind-flotation tests were conducted on a Ni flotation tailings stream. Batch tests revealed that the Mg grade decreased dramatically in the concentrate products. Pilot-scale testing of a continuous centrifugal concentrator（Knelson CVD6） on the flotation tailings revealed that a concentrate with a low mass yield, low Mg content, and high Ni upgrade ratio could be achieved. Under optimum conditions, a concentrate at 6.7% mass yield was obtained with 0.85% Ni grade at 12.9% Ni recovery and with a low Mg distribution（1.7%）. Size partition curves demonstrated that the CVD also operated as a size classifier, enhancing the rejection of talc fines. Overall, the CVD was capable of rejecting Mg-bearing minerals. Moreover, an opportunity exists for the novel use of centrifugal-gravity concentration for scavenging flotation tailings and/or after comminution to minimize amount of Mg-bearing minerals reporting to flotation.

关键词： flotation base metals talc centrifugation gravity concentrators

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Machine learning insights concerning inflammatory and liver-related risk comorbidities in non-communicable and viral diseases

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World Journal of Gastroenterology 2022年第44期28卷 6230-6248页

作者： J Alfredo Martínez Marta Alonso-bernáldez Diego Martínez-Urbistondo Juan A Vargas-Nuñez Ana Ramírezde Molina Alberto Dávalos Omar Ramos-Lopez Precision Nutrition and Cardiometabolic Health Madrid Institute of Advanced Studies-Food InstituteMadrid 28049Spain Department of Internal Medicine Hospital Universitario HM SanchinarroMadrid 28050Spain Servicio de Medicina Interna Hospital Universitario Puerta de Hierro MajadahondaMadrid 28222MajadahondaSpain Molecular Oncology and Nutritional Genomics of Cancer Madrid Institute of Advanced Studies-Food InstituteMadrid 28049Spain Laboratory of Epigenetics of Lipid Metabolism Madrid Institute of Advanced Studies-Food InstituteMadrid 28049Spain Medicine and Psychology School Autonomous University of Baja CaliforniaTijuana 22390Baja CaliforniaMexico

The liver is a key organ involved in a wide range of functions,whose damage can lead to chronic liver disease(CLD).CLD accounts for more than two million deaths worldwide,becoming a social and economic burden for most *** the different factors that can cause CLD,alcohol abuse,viruses,drug treatments,and unhealthy dietary patterns top the *** conditions prompt and perpetuate an inflammatory environment and oxidative stress imbalance that favor the development of hepatic *** stages of fibrosis can eventually lead to cirrhosis or hepatocellular carcinoma(HCC).Despite the advances achieved in this field,new approaches are needed for the prevention,diagnosis,treatment,and prognosis of *** this context,the scientific community is using machine learning(ML)algorithms to integrate and process vast amounts of data with unprecedented *** techniques allow the integration of anthropometric,genetic,clinical,biochemical,dietary,lifestyle and omics data,giving new insights to tackle CLD and bringing personalized medicine a step *** review summarizes the investigations where ML techniques have been applied to study new approaches that could be used in inflammatoryrelated,hepatitis viruses-induced,and coronavirus disease 2019-induced liver damage and enlighten the factors involved in CLD development.

关键词： Machine learning Liver inflammation Liver disease Viral diseases Comorbidity

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SMEFT is falsifiable through multi-Higgs measurements(even in the absence of new light particles)

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Communications in Theoretical Physics 2023年第9期75卷 69-74页

作者： Raquel Gómez-Ambrosio Felipe J Llanes-Estrada Alexandre Salas-bernárdez Juan J Sanz-Cillero Dipartimento di Fisica Univ.di Torinoand INFNSezione di TorinoVia P.Giuria 1Torino10125Italy 2Univ.Complutense de MadridDept.Fisica Teorica and IPARCOSPlaza de las Ciencias 1Madrid28040Spain

By embedding the Standard Model effective field theory(SMEFT) in the more general Higgs effective field theory(HEFT), we expose correlations among the coefficients of the latter that, if found to be violated in future data, would lead to the experimental falsification of the SMEFT framework. These are derived from the necessary symmetric point of HEFT and analyticity of the SMEFT Lagrangian that allows the construction of the SMEFT expansion, as laid out by other groups, and properties at that point of the Higgs-flare function F(h) coupling Goldstone and Higgs bosons, of the Higgs potential V(h) and of the Higgs-top quark coupling function G(h).

关键词： SMEFT HEFT EFT effective field theories electroweak LHC quantum field theories

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A 3-MONTH RANDOMIZED CONTROLLED TRIAL OF BIMATOPROST (LUMIGAN) VERSUS TIMOLOL/DORZOLAMIDE (COS - OPT) IN PATIENTS WITH GLAUCOMA OR OCULAR HYPERTENSION A. Coleman, P. bern-

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国际眼科杂志 2003年第1期3卷 27-28页

作者： A.Coleman P.bern-stein S.Whitcup Jules Stein Institute/UCLA CAAllergan Inc. CA USAAllergan Inc. CA USA

Objective: To compare efficacy and safety of bimato-prost (Lumigan) QD vs timolol/dorzolamide (Cosopt) BID. Methods: Randomized, double-masked, multicenter 3-month clinical trial. 177 patients with glaucoma or ocular hypertension with uncontrolled intraocular pressure (IOPs between 22 and 34 mmHg) after at least 2 weeks of timolol 0.5% monotherapy

关键词：青光眼高眼压治疗 Bimatoprost 噻吗洛尔/多佐胺随机对照试验

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Morphology Study of Particle Breakage Mechanisms in a Horizontal Stirred Mill: Automated and Manual Point Counting Approaches

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Journal of Minerals and Materials Characterization and Engineering 2021年第5期9卷 528-543页

作者： Reem Roufail bern Klein Systems Design Engineering University of Waterloo Waterloo Canada Norman B. Keevil Institute of Mining Engineering University of British Columbia Vancouver Canada

High-speed stirred mills are utilized to grind particles below 10mm. Grinding sulphide minerals to as low as 10mm achieve adequate mineral liberation for successful downstream mineral processing operations, such as flotation and leaching. Particle breakage mechanism such as fracture or abrasion, determines the morphological surface features of the product particles. It is anticipated that particles, which break along grain boundaries (intergranular) produce rough surfaces, whereas particles that break across the grain boundaries (transgranular) possess smoother surfaces. In this study, particles are ground in a stirred mill and their morphological features were analyzed using automated and manual detection methods. Literature and conventional belief are that high-speed stirred mills break particles by attrition. This paper showed that fracture is also an important breakage mechanism along with attrition. Breakage mechanism is a factor of input stress intensity, in the form of the mill agitator speed, and type of mineral. It is observed that at higher agitator speed galena fractures along the grain boundaries, whereas quartz, abrade across the grain boundaries.

关键词： Comminution Morphology Breakage Mechanism Stirred Mill Grinding Manual Point Counting

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Detection of Optical Stability of chiral 2-Methylbutyric Acid in Gas Phase with Quadrupole-Quistor-Quadrupole Mass Spectrometer

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Chinese Chemical Letters 1999年第3期10卷 213-214页

作者： Yuan Jiang PANI U.P.SCHLUNEGGERZ(Department of Chemistry, Zhejiang University, Hangzhou 310027)(Department of Chemistry and Biochemistry. berne University, CH-30 12 bern,Switzerland) Department of Chemistry Zhejiang University Hangzhou 310027 China Dept. of Chemistry and Biochemistry Berne University CH-3012 Bern Switzerland

Optical stability of chiral 2-methylbutyric acid in gas phase has been detected with Quadrupole-Quistor-Quadrupole tandem mass spectrometer in combination with deuteration. The results show that these compounds are op... 详细信息

关键词： 2-methylbutyric acid optical stability mass spectrometric detection

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