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Systematic characterization of cleanroom-free fabricated macrovalves,demonstrating pumps and mixers for automated fluid handling tuned for organ-on-chip applications

引用

Microsystems & Nanoengineering 2022年第3期8卷 163-176页

作者： Elsbeth G.B.M.Bossink Anke R.Vollertsen Joshua T.loessberg-Zahl Andries D.van der Meer loe l.segerink Mathieu Odijk BIOs lab on a Chip Group MESA+instituteTechnical Medical CenterMax Planck Institute for Complex Fluid DynamicsUniversity of TwenteEnschedeThe Netherlands Applied Stem Cell Technologies Group Technical Medical CenterUniversity of TwenteEnschedeThe Netherlands

Integrated valves enable automated control in microfuidic systems,as they can be applied for mixing,pumping and compartmentalization *** automation would be highly valuable for applications in organ-on-chip(OoC)***,OoC systems typically have channel dimensions in the range of hundreds of micrometers,which is an order of magnitude larger than those of typical microfluidic *** most-used fabrication process for integrated,normally open polydimethylsiloxane(PDMS)valves requires a reflow photoresist that limits the achievable channel *** addition,the low stroke volumes of these valves make it challenging to achieve flow rates of microliters per minute,which are typically required in OoC ***,we present a mechanical'macrovalve'fabricated by multilayer soft lithography using micromilled direct *** demonstrate that these valves can close off rounded channels of up to 700μm high and 1000μm ***,we used these macrovalves to create a peristaltic pump with a pumping rate of up to 48μl/min and a mixing and metering device that can achieve the complete mixing of a volume of 6.4μl within only 17 *** initial cell culture experiment demonstrated that a device with integrated macrovalves is biocompatible and allows the cell culture of endothelial cells over multiple days under continuous perfusion and automated medium refreshment.

关键词： pumping mixer pump

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