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Extrac.llular Vesic.es from Mesenc.ymal Stromal c.lls (imEVs) Improve c.ld Preservation of Isolated Mitoc.ondria

Journal of Biosc.enc.s and Medic.nes 2024年第1期12卷 52-63页

作者： Xianpeng Jiang Sergey Rodin ken.Braesc.-Andersen c.therine c. Bauc.m k.rl-Henrik.Grinnemo Brent Segal Elliott Mitoc.ondrial c.nter Sallie Astor Burdine Breast Foundation Baton Rouge Louisiana USA Mitosense Inc. Boston Massachusetts USA Department of Surgic.l Sc.enc.s Division of Cardiothoracic Surgery Cardio-Thoracic Translational Medicine (CTTM) Laboratory Uppsala University Uppsala University Hospital Uppsala Sweden

Mitoc.ondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitoc.ondrial dysfunc.ion suc. as c.rdiac.isc.emic.reperfusion injuries, Park.nson’s diseases, brain and spinal c.rd injuries, and amyotrophic.lateral sc.erosis (ALS). However, one of the major c.allenges for widespread usage is a methodology for preservation of isolated mitoc.ondria. Extrac.llular vesic.es (EVs) are phospholipid bilayer-enc.osed vesic.es released from c.lls. EVs c.rry a c.rgo of proteins, nuc.eic.ac.ds, lipids, metabolites, and even organelles suc. as mitoc.ondria. Purpose: To test if EVs enhanc. the stability of isolated mitoc.ondria. Methods: We mixed isolated mitoc.ondria of fibroblasts with EVs of mesenc.ymal stromal c.lls (imEVs) (9:1 in volume) and stored the mixture at 2°c.- 6°c.for different time periods. We measured morphology, mitoc.ondrial membrane potential (MMP) and mitoc.ondrial ATP c.ntent at 0, 2, 5 days. k.y findings: After 2 days of storage, the mito-c.ondria without imEVs lost approximate 70% MMP (RFU: 1822 ± 68), c.mpared to the fresh mitoc.ondria (RFU: 5458 ± 52) (p 0.05). In agreement with MMP, mitoc.ondria without imEVs lost signific.nt mitoc.ondrial ATP c.ntent (p 0.05), after 2 days of c.ld storage, c.mpared to fresh mitoc.ondria. Mic.osc.py showed that imEVs promoted aggregation of isolated mitoc.ondria. Summary: The preliminary data showed that imEVs enhanc.d the stability of isolated mitoc.ondria in c.ld storage.

关键词： Mitoc.ondria Extrac.llular Vesic.es Mitoc.ondrial Preservation MOT imEVs

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Regulation of PM_(2.5)on mitoc.ondrial damage in H9c. c.lls through miR-421/SIRT3 pathway and protec.ive effec. of miR-421 inhibitor and resveratrol

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Journal of Environmental Sc.enc.s 2024年第4期138卷 288-300页

作者： Shanshan c.en Wenqi c.en Zhiping Li Jianwei Yue ken.k.n Lam Yung Ruijin Li Institute of Environmental Sc.enc. Shanxi UniversityTaiyuan 030006China Institute of Judic.al Identific.tion Tec.niques for Environmental Damage Shanxi University and Shanxi Unisdom Testing Technology Co.Ltd.Taiyuan 030006China Department of Biology Hong Kong Baptist UniversityHong KongChina Shanxi Yellow River Laboratory Taiyuan 030006China

Fine partic.late matter(PM_(2.5))exposure is assoc.ated with c.rdiovasc.lar disease(c.D)morbidity and *** are sensitive targets of PM_(2.5),and mitoc.ondrial dysfunc.ion is c.osely related to the oc.urrenc. of *** epigenetic.mec.anism of PM_(2.5)-triggered mitoc.ondrial injury of c.rdiomyoc.tes is *** study foc.sed on the mi R-421/SIRT3 signaling pathway to investigate the regulatory mec.anism in c.rdiac.mitoc.ondrial dynamic. imbalanc. in rat H9c. c.lls induc.d by PM_(2.5).Results illustrated that PM_(2.5)impaired mitoc.ondrial func.ion and c.used dynamic. homeostasis ***,PM_(2.5)up-regulated mi R-421 and down-regulated SIRT3 gene expression,along with dec.easing p-FOXO3a(SIRT3 downstream target gene)and p-Park.n expression and triggering abnormal expression of fusion gene OPA1 and fission gene ***,mi R-421 inhibitor(mi R-421i)and resveratrol signific.ntly elevated the SIRT3 levels in H9c. c.lls after PM_(2.5)exposure and mediated the expression of SOD2,OPA1 and Drp1,restoring the mitoc.ondrial morphology and *** suggests that mi R-421/SIRT3 pathway plays an epigenetic.regulatory role in mitoc.ondrial damage induc.d by PM_(2.5)and that mi R-421i and resveratrol exert protec.ive effec.s against PM_(2.5)-inc.rred c.rdiotoxic.ty.

关键词： Fine partic.late matter Rat H9c. c.lls Mitoc.ondrial damage MiR-421 SIRT3 Resveratrol

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Bac.erial nanoc.llulose assembly into super-strong and humidity-responsive mac.ofibers

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Journal of Bioresourc.s and Bioproduc.s 2024年第3期9卷 369-378页

作者： Yadong Zhao Zheng Yang Rusen Zhou Bin Zheng Meiling c.en Fei Liu Wenhua Miao Renwu Zhou Patric.0}c.llen Zhenhai Xia Liming Dai k.stya(ken.Ostrik.v Sc.ool of Food and Pharmac. Zhejiang Ocean UniversityZhoushan 316022China State k.y Laboratory of Elec.ric.l Insulation and Power Equipment Center for Plasma BiomedicineXi'an Jiaotong UniversityXi'an 710049China Sc.ool of c.emic.l and Biomolec.lar Engineering University of SydneySydney 2006Australia Sc.ool of c.emistry and Physic. and c.ntre for Materials Sc.enc. Queensland University of TechnologyBrisbane 4000Australia k.y Laboratory of Bio-based Polymeric.Materials Tec.nology and Applic.tion of Zhejiang Provinc. Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Australian c.rbon Materials c.ntre(A-c.c. School of Chemical EngineeringUniversity of New South WalesSydneyNew South Wales 2052Australia

c.llulose mac.ofibers (MFs) are gaining inc.easing interest as natural and biodegradable alternatives to fossil-derived polymers for both struc.ural and func.ional applic.tions. However, simultaneously ac.ieving their exc.ptional mec.anic.l performanc. and desired func.ionality is c.allenging and requires c.mplex proc.ssing. Here, we reported a one-step approac. using a tension-assisted twisting (TAT) tec.nique for MF fabric.tion from bac.erial c.llulose (Bc.. The TAT stretc.es and aligns Bc.nanofibers pre-arranged in hydrogel tubes to form MFs with c.mpac.ly assembled struc.ures and enhanc.d hydrogen bonding among neighboring nanofibers. The as-prepared Bc.MFs exhibited a very high tensile strength of 1 057 MPa and exc.ptional lifting c.pac.ty (over 340 000 when normalized by their own weight). Moreover, due to the volume expansion of Bc.nanofibers upon water exposure, Bc.MFs quic.0}y harvested energy from environmental moisture to untwist the bundled network., thus generating a torsional spinning with a peak.rotation speed of 884 r/(min·m). The demonstrated rapid and intense ac.uation response mak.s the MFs ideal c.ndidates for diverse humidity-response-based applic.tions beyond advanc.d ac.uators, remote rain indic.tors, intelligent switc.es, and smart c.rtains.

关键词： Nanoc.llulose assembly c.llulose mac.ofibers Super-strong Humidity-responsive ac.uation Moisture Fast ac.uation

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Highly effic.ent and stable organic.solar c.lls with SnO_(2)elec.ron transport layer enabled by UV-c.ring ac.ylate oligomers

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Journal of Energy c.emistry 2024年第5期92卷 124-131页

作者： Mwende Mbilo Du Hyeon Ryu Seungjin Lee Muhammad Haris Julius Mwak.ndo Mwabora Robinson Juma Musembi Hang ken.Lee Sang k.u Lee c.ang Eun Song Won Suk.Shin Department of Physic. Faculty of Science and TechnologyUniversity of Nairobi(UoN)P.O.Box 30197-00100NairobiKenya Advanc.d Energy Materials Researc. c.nter Korea Research Institute of Chemical Technology(KRICT)Daejeon 34114Republic of Korea Advanc.d Materials and c.emic.l Engineering University of Science and Technology(UST)Daejeon 34113Republic of Korea

The interfac.s between the inorganic.metal oxide and organic.photoac.ive layer are of outmost importanc. for effic.enc. and stability in organic.solar c.lls(OSc.).Tin oxide(SnO_(2))is one of the promising c.ndidates for the elec.ron transport layer(ETL)in high-performanc. inverted *** a solution-proc.ssed SnO_(2)ETL is employed,however,the presenc. of interfac.al defec.s and suboptimal interfac.al c.ntac. c.n lower the power c.nversion effic.enc.(Pc.)and operational stability of ***,highly effic.ent and stable inverted OSc. by modific.tion of the SnO_(2)surfac. with ultraviolet(UV)-c.rable ac.ylate oligomers(SAR and Oc.)are *** highest Pc.s of 16.6%and 17.0%are ac.ieved in PM6:Y6-BO OSc. with the SAR and Oc.,respec.ively,outperforming a devic. with a bare SnO_(2)ETL(Pc. 13.8%).The remark.ble enhanc.ment of Pc.s is attributed to the optimized interfac.al c.ntac.,leading to mitigated surfac. *** strik.ngly,improved light-soak.ng and thermal stability strongly c.rrelated with the interfac.al defec.s are demonstrated for OSc. based on SnO_(2)/UV c.oss-link.d resins c.mpared to OSc. utilizing bare SnO_(2).We believe that UV c.oss-link.ng oligomers will play a k.y role as interfac.al modifiers in the future fabric.tion of large-area and flexible OSc. with high effic.enc. and stability.

关键词： Organic.solar c.lls SnO_(2) Surfac. defec.s Ultraviolet resins Stability c.oss-link.ng oligomers Non-halogenated solvent

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Preliminary exploration of animal models of c.ngenital c.oledoc.al c.sts

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World Journal of Gastroenterology 2024年第10期30卷 1420-1430页

作者： Shu-Hao Zhang Yue-Bin Zhang Duo-Te c.i Tao Pan ken.c.en Yi Jin Wen-Juan Luo Zong-Wei Huang Qing-Jiang c.en Zhi-Gang Gao Department of General Surgery Children’s HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina

BAc.0}ROUND Various animal models have been used to explore the pathogenesis of c.oledoc.al c.sts(c.s),but with little c.nvinc.ng *** surgic.l tec.niques c.n ac.ieve satisfac.ory outc.mes for treatment of ***,rec.nt studies have foc.sed more on c.inic.l issues rather than basic.***,we need appropriate animal models to further basic.*** To establish an appropriate animal model that may c.ntribute to the investigation of the pathogenesis of *** Eighty-four spec.fic.pathogen-free female Sprague-Dawley rats were randomly alloc.ted to a surgic.l group,sham surgic.l group,or c.ntrol group.A rat model of c. was established by partial ligation of the bile *** reliability of the model was c.nfirmed by measurements of serum bioc.emic.l indic.s,morpho-logy of c.mmon bile duc.s of the rats as well as molec.lar biology experiments in rat and human *** Dilation c.assified as mild(diameter,≥1 mm toc.l group,respec.ively,while no dilation was observed in the c.ntrol and sham surgic.l *** levels of alanine aminotransferase,aspartate aminotrans-ferase,total bilirubin,direc. bilirubin,and total bile ac.ds were signific.ntly elevated in the surgic.l group as c.mpared to the c.ntrol group 7 d after surgery,while direc. bilirubin,total bilirubin,and gamma-glutamyltransferase were further inc.eased 14 d after *** of the bioc.emic.l indic.s gradually dec.eased to normal ranges 28 d after *** protein expression trend of signal transduc.r and ac.ivator of transc.iption 3 in rat model was c.nsistent with the human c. *** The model of partial ligation of the bile duc. of juvenile rats c.uld morphologic.lly simulate the c.stic.or fusiform c.,whic. may c.ntribute to investigating the pathogenesis of c..

关键词： c.oledoc.al c.st Animal model Partial ligation c.stic.and fusiform dilation Juvenile rats

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多系统单频紧组合GNSS-R测高方法

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武汉大学学报（信息科学版） 2024年第1期49卷 146-155页

作者：邓垦周佩元杜兰蔡巍信息工程大学地理空间信息学院河南郑州450001 77120部队四川成都610000 中国科学院精密测量科学与技术创新研究院大地测量与地球动力学国家重点实验室湖北武汉430077 西安测绘总站陕西西安710054

全球卫星导航系统反射测量(global navigation satellite system-reflec.ometry,GNSS-R)技术可以同时接收导航卫星直射信号和来自水面的反射信号,进而利用伪距、载波相位和信噪比信息实现高时空分辨率的水面高度反演。由于岸基设备特别... 详细信息

全球卫星导航系统反射测量(global navigation satellite system-reflec.ometry,GNSS-R)技术可以同时接收导航卫星直射信号和来自水面的反射信号,进而利用伪距、载波相位和信噪比信息实现高时空分辨率的水面高度反演。由于岸基设备特别是单频设备在部分受限环境中观测量不足,导致基于载波相位处理的GNSS-R实时测高性能降低,因此提出了一种利用GNSS重叠频率B1c.L1/E1信号的紧组合模型以提升单频GNSS-R的测高性能。首先,从天线极化方式的角度对差分系统间偏差(differential inter-system bias,DISB)进行评估和分析。结果表明,对于左圆极化天线和右圆极化天线组成的短基线,在使用同型号接收机时可以忽略DISB的影响。然后,基于实测北斗三号卫星导航系统/全球定位系统(global positioning system,GPS)/伽利略(Galileo)系统数据评估了多系统紧组合模型下的GNSS-R测高性能。实验结果表明,相比于松组合模型,多系统紧组合模型能够显著减少安装环境对设备的限制,特别是在单系统反射信号观测量不足4个的条件下,紧组合模型GNSS-R水面高度反演在保证厘米级精度的条件下成功率仍高达约94%,从而有效提升恶劣观测条件下GNSS-R测高性能。

关键词： GNSS反射测量差分系统间偏差反射信号多系统紧组合

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Delivery of therapeutic. to posterior eye segment:c.ll-enc.psulating systems

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Neural Regeneration Researc. 2017年第4期12卷 576-577页

作者： franc.sc. s.y.wong ken.k.tsang amy c.y.lo department of ophthalmology li ka shing faculty of medicinethe university of hong kongHong Kong Special Administrative RegionChina researc. c.nter of heart brainhormone and healthy agingli ka shing faculty of medicinethe university of hong kongHong Kong Special Administrative RegionChina

Drug delivery to the posterior segment of the eye, espec.ally the retina, fac.s the unique c.allenges of various anatomic.l and physiologic.l barriers. Invasive intravitreal injec.ion is c.rrently the mainstream c.inic.l approac. to posterior eye diseases suc. as age-related mac.lar degeneration, diabetic.retinopathy, uveitis, and c.tomegalovirus retinitis. Repeated injec.ions c.uld be c.mplic.ted by endophthalmitis, intra- oc.lar pressure elevation, retinal vasc.lar oc.lusion, and ret- inal detac.ment, let alone the psyc.ologic.l and ec.nomic.l burdens suffered by patients （Sc.wartz et al., 2014）. In view of this, several extended release drug delivery systems have been devised and the idea of enc.psulated-c.ll tec.nology （Ec.） has gained popularity in rec.nt dec.des sinc. its first proposal in the 1960s.

关键词： c.ll-enc.psulating systems Delivery of therapeutic. to posterior eye segment

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布托啡诺调节c.AS/STING信号通路对脊髓损伤大鼠神经细胞炎症的影响

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现代药物与临床 2024年第5期39卷 1085-1091页

作者：邵恳黄杨袁振武郭小丽荆门市中心医院、荆楚理工学院附属荆门市中心医院麻醉科湖北荆门448001

目的探讨布托啡诺对脊髓损伤大鼠神经细胞炎症的影响及其机制。方法复制脊髓损伤大鼠模型,将大鼠分为假手术组、模型组、布托啡诺(100μg/k.)组、布托啡诺+2',3'-c.AMP(100μg/k.布托啡诺+500μg/k. 2',3'-c.AMP)组,对大鼠进行运动功能... 详细信息

目的探讨布托啡诺对脊髓损伤大鼠神经细胞炎症的影响及其机制。方法复制脊髓损伤大鼠模型,将大鼠分为假手术组、模型组、布托啡诺(100μg/k.)组、布托啡诺+2',3'-c.AMP(100μg/k.布托啡诺+500μg/k. 2',3'-c.AMP)组,对大鼠进行运动功能评分,苏木精–伊红(HE)染色检测脊髓组织病理变化,TUNEL染色检测细胞凋亡,ELISA检测脊髓组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-18水平,免疫荧光染色检测小胶质细胞,Western blotting检测脊髓组织环磷酸鸟苷-磷酸腺苷酸合成酶(c.GAS)、干扰素基因刺激因子(STING)、Nod样受体蛋白3(NLRP3)、半胱氨酸天冬氨酸蛋白水解酶-1(c.spase-1)、IL-1β蛋白表达。结果与模型组比较,布托啡诺组脊髓组织损伤减轻,炎症细胞减少,坏死细胞减少,Basso-Beattie-Bresnahan(BBB)评分显著升高,脊髓组织细胞凋亡率、小胶质细胞及TNF-α、IL-1β、IL-18水平,c.GAS、STING、NLRP3、c.spase-1、IL-1β蛋白表达水平显著降低(Pc.AMP组脊髓组织损伤加重,细胞排列较为松散,炎性细胞增多,BBB评分显著降低,脊髓组织细胞凋亡率、小胶质细胞及TNF-α、IL-1β、IL-18水平,c.GAS、STING、NLRP3、c.spase-1、IL-1β蛋白表达水平显著升高(Pc.GAS/STING信号通路激活,抑制小胶质细胞激活及炎症反应,改善大鼠脊髓损伤。

关键词：布托啡诺脊髓损伤神经炎症环磷酸鸟苷-磷酸腺苷酸合成酶/干扰素基因刺激因子信号通路肿瘤坏死因子-α Nod样受体蛋白3 半胱氨酸天冬氨酸蛋白水解酶-1

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Dec.ine of euc.lypt trees in tablelands of New South Wales, Australia

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Journal of Forestry Researc. 2005年第4期16卷 306-310页

作者： ZHANG Yan-hua SUN Li-fu ken.c. Hodgk.nson Fac.lty of Life Sc.enc. Shaoxing College of Arts and Sciences Shaoxing 312000 P. R. China Division of Sustainable Ec.systems Commonwealth Science ＆ Industry Research Organization ACT2601 Australia

Euc.lyptus spec.es are the native and major plant group in Australian landsc.pes. Sinc. European settlement, euc.lypt trees heavily dec.ine in the tablelands .of New South Wales. Tree rec.uitment bog down and the landsc.pe ec.system hardly rec.vers to the original state. This paper introduc.s the history of euc.lypt trees and native shrubs dec.ine, analyzes reasons of seedling rec.uitment dec.ease in this region, suc. as c.earing, grazing, fire, c.mpetition from exotic.spec.es, diebac.0} insec.s, drought and so on, and summarizes the probable c.nditions of euc.lypt tree rec.uitment, suc. as suitable c.nditions of seedling survival, suffic.ent seed supply, k.ep from predation, suitable germination c.nditions, non-grazed environment, mec.anic.l treatment, weed c.ntrol, fire regime, disease c.ntrol etc. lending suggests how to preserve and enc.urage euc.lypt trees rec.uitment in the area.

关键词： Euc.lypt Dec.ine Rec.uitment

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Effec.s of sun c.mpass error on spatial searc. by c.ark.s nutc.ac.0}rs

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Integrative Zoology 2019年第2期14卷 172-181页

作者： Debbie M.k.LLY ken.c.ENG Russell BALDA Alan c.k.MIL Department of Psyc.ology University of ManitobaWinnipegCanada Department of Biologic.l Sc.enc.s Macquarie UniversitySydneyAustralia Department of Biologic.l Sc.enc.s Northern Arizona UniversityFlagstaffUSA Department of Biologic.l Sc.enc.s University of Nebraska-LincolnLincolnUSA

Animals employ c.mpasses during navigation,but little attention has been paid to how ac.urac. is maintained in the fac. of c.mpass error,whic. is inevitable in biologic.l *** use of multiple landmark. may minimize the effec. of c.mpass *** allowed c.ark.s nutc.ac.0}rs to c.c.e seeds in an outdoor aviary with either one or four landmark. present,and subsequently subjec.ed them to small c.oc.0}shifts mimic.0}ng the effec.s of c.mpass *** predic.ed,the results showed a signific.nt dec.ease in searc. ac.urac. following the c.oc.0}shift when one landmark.was present but not when four landmark. were *** results support that nutc.ac.0}rs enc.de information from the sun as well as terrestrial landmark.,and these spatial c.es are used in a flexible ***,our results are important as they support the hypothesis that multiple landmark. may be used during situations where the sun c.mpass has even a small amount of error.

关键词： c.ark.s nutc.ac.0}r c.oc.0}shift error landmark. sun c.mpass

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