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Contents Variation Analysis ofγ-Amino Butyric Acid in Semen sojae praeparatum Fermentation Using Online Pre-Column Derivatization-HPLC

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Wuhan University Journal of Natural Sciences 2021年第1期26卷 55-61页

作者： WENG Meizhi HE Jing REN Jiaxiu LIANG Yonghong CHEN Qingfeng SU Mingsheng wang liyuan XIE Xiaomei College of Traditional Chinese Medicine Jiangxi University of Traditional Chinese MedicineNanchang 330004JiangxiChina Institute of Translational Medicine Nanchang UniversityNanchang 330031JiangxiChina Key Laboratory of Modem Preparation ofTCM Ministry of EducationJiangxi University of Traditional Chinese MedicineNanchang 330004JiangxiChina

This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese *** performance liquid chromatography(HPLC)was used and GABA was derivatized by online pre-column derivatization with o-phthalaldehyde(OPA).To validate this method,the precision,stability,repeatability and recovery were *** the concentration range from 0.0125 to 0.400 mg/m L,the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient(R2)of *** high levels of GABA exist in SSP and the content changes of GABA at different time points during the fermenting process were *** the"yellow cladding"stage,GABA level was very low or even undetectable;the"secondary fermentation"stage witnessed a rapid increase of GABA content to 1.39-5.52 mg/g,which remained stable after 18 days of"secondary fermentation".This study demonstrated that GABA was generated at the"secondary fermentation"stage,revealing the significance and rationality of the"secondary fermentation"stage in the fermenting process of *** the other hand,it suggested the downside of taking soy isoflavones as the only measurement in existing quality assessment and optimization approach for the fermenting process of SSP.

关键词： Semen sojae praeparatum γ-amino butyric acid(GABA) "secondary fermentation"process online pre-column derivatization-high performance liquid chromatography

A proteome-wide screen identifies valosin-containing protein as an essential regulator of podocyte endoplasmic reticulum stress

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Chinese Science Bulletin 2012年第20期57卷 2493-2505页

作者： HUANG ZhiYong HONG Quan XUE Peng PAUL Goulding FENG Zhe wang liyuan MEI Yan WU LingLing CHEN XiangMei WU Di State Key Laboratory of Kidney Disease(2011DAV00088) Kidney Department&Institute of NephrologyDivision of Clinical Internal MedicineChinese People’s Liberation Army General HospitalBeijing 100853China Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Nonlinear Dynamics Ltd Keel HouseGarth HeadsNewcastle upon TyneNE12JEUK

To investigate proteins expressed in the renal tissue of the passive Heymann nephritis (pHN) rat model,we prepared pHN rat models with anti-FxA1 serum and analyzed the proteins differentially expressed in the kidney tissue with label-free liquid chromatography-tandem mass *** then analyzed in depth the endoplasmic reticulum stress (ERS)-related protein using an online bioinformatics ***-one differential proteins and their annotations were *** Ontology (GO) function analysis showed that 16 proteins were involved in cellular metabolism and 22 were proteins related to catalytic activity,including protein folding or ***-GO networks indicated that VCP could interact with the ERS marker HSPa5,with both involved in a single *** inhibition of podocyte VCP by RNAi under normal conditions,the HSPa5 expression level did not change,but when the cell was subjected to ERS by tunicamycin,HSPa5 expression significantly increased with RNAi of VCP when compared with the tunicamycin-treated *** results showed that ERS plays an important role in podocyte injury of membranous nephropathy and is mediated by an HSPa5-VCP signaling pathway,in which the most predominant proteins are those related to cellular metabolism and catalytic activity.

关键词：蛋白质折叠内质网应激细胞损伤 Networks公司宽屏幕标识大鼠模型催化活性

DTZH1505:大规模开源中文普通话语音库

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计算机工程与应用 2022年第11期58卷 295-301页

作者：王东王丽媛王大亮齐红威西藏民族大学信息工程学院陕西咸阳712082 数据堂(北京)科技股份有限公司北京100192

近年来,深度学习在语音识别领域取得了突破性进展,并推动语音识别技术广泛应用到人们的日常生活中。语音识别模型的进一步优化需要更大规模标定数据的驱动,然而,目前开源的语音数据集规模仍太小,语料多为偏向书面用语的新闻类长文本。... 详细信息

近年来,深度学习在语音识别领域取得了突破性进展,并推动语音识别技术广泛应用到人们的日常生活中。语音识别模型的进一步优化需要更大规模标定数据的驱动,然而,目前开源的语音数据集规模仍太小,语料多为偏向书面用语的新闻类长文本。针对人机交互、智能客服等热门语音识别应用,通过众包模式采集朗读式语音,构建并开源了迄今为止最大规模的中文普通话语音数据集DTZH1505。数据集记录了6408位来自中国八大方言地域、33个省份的说话人的自然语音,时长达1505 h,语料内容涵盖社交聊天、人机交互、智能客服以及车载命令等,可广泛用于语料库语言学、会话分析、语音识别、说话人识别等研究。开展一系列基准语音识别实验,实验结果表明:相较于同规模中文语音数据集aishell2,基于此数据集训练的语音识别模型效果更好。

关键词：中文普通话语音库开源数据语音识别深度学习音素平衡

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英国商学院基于案例的国际化小组研讨方法

中国ESP研究 2023年第4期 104-113,126-127页

作者：王力媛江西财经大学

在全球商科教育国际化背景下,来自世界各国的学生进行商务案例小组研讨,通过合作探究新知,提升风险预判能力和跨文化沟通能力。本研究跟踪一组中英分地培养国际商务本科生在英国商学院参与混合式案例讨论的过程,重点分析了中国留学生话... 详细信息

在全球商科教育国际化背景下,来自世界各国的学生进行商务案例小组研讨,通过合作探究新知,提升风险预判能力和跨文化沟通能力。本研究跟踪一组中英分地培养国际商务本科生在英国商学院参与混合式案例讨论的过程,重点分析了中国留学生话题操控及其对他人观点的回应方式,挖掘出螺旋式、个人式和累积式三种小组讨论方法,展示了参与者独特的自我反思的自主学习策略。研究结果对国内外商科教育项目的ESP课程设置有一定启示。

关键词：案例小组讨论策略商务英语英国课堂中国学生

Editing of an effector gene promoter sequence impacts plant-Phytophthora interaction

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Journal of Integrative Plant Biology 2020年第3期62卷 378-392页

作者： Sylvans Ochola Jie Huang Haider Ali Haidong Shu Danyu Shen Min Qiu liyuan wang Xi Li Han Chen Alex Kange Dinah Qutob Suomeng Dong Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education) Nanjing 210095China Department of Math and Science Walsh UniversityNorth CantonOH 44720USA

Pathogen avirulence(Avr) effectors interplay with corresponding plant resistance(R) proteins and activate robust plant immune responses. Although the expression pattern of Avr genes has been tied to their functions for a long time, it is still not clear how Avr gene expression patterns impact plant-microbe interactions. Here, we selected Ps Avr3b, which shows a typical effector gene expression pattern from a soybean root pathogen Phytophthora sojae. To modulate gene expression, we engineered Ps Avr3b promoter sequences by in situ substitution with promoter sequences from Actin(constitutive expression), Ps XEG1(early expression), and Ps NLP1(later expression) using the CRISPR/Cas9. Ps Avr3b driven by different promoters resulted in distinct expression levels across all R the tested infection time points. Importantly, those mutants with low Ps Avr3b expression successfully colonized soybean plants carrying the cognate R gene Rps3b. To dissect the difference in plant responses to the Ps Avr3b expression level, we conducted RNA-sequencing of different infection samples at 24 h postinfection and found soybean immune genes,including a few previously unknown genes that are associated with resistance. Our study highlights that fine-tuning in Avr gene expression impacts the compatibility of plant disease and provides clues to improve crop resistance in disease control management.

关键词： CRISPR/Cas9 infection soybean

‘热研2号’柱花草对不同亲缘植物根系分泌物的生长响应

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草业科学 2022年第2期39卷 309-317页

作者：王俐媛王坚赵元辰许超李雪枫热带特色林木花卉遗传与种质创新教育部重点实验室/海南大学林学院海南海口570228 海南大学动物科技学院海南海口570228

植物间彼此存在交流行为,可以通过身份识别与亲缘植物进行相互合作与竞争。本试验旨在探究‘热研2号’柱花草(Stylosanthes guianensis‘Reyan No.2’)对不同亲缘植物根系分泌物的识别差异。将‘热研2号’柱花草分别在7种含有不同亲缘... 详细信息

植物间彼此存在交流行为,可以通过身份识别与亲缘植物进行相互合作与竞争。本试验旨在探究‘热研2号’柱花草(Stylosanthes guianensis‘Reyan No.2’)对不同亲缘植物根系分泌物的识别差异。将‘热研2号’柱花草分别在7种含有不同亲缘关系柱花草根系分泌物的营养液中培养,研究自我、种内近缘亲属、种间远缘亲属根系分泌物对‘热研2号’柱花草幼苗生长状况的影响。结果表明:与自我根系分泌物处理组相比,近亲处理组的侧根数量、总生物量、茎叶生物量和根生物量(鲜重)的平均值均显著增加(P0.05);远亲处理组的根长、侧根数量、根生物量和根冠比的平均值均显著增加(P‘热研24号’>‘细茎’>‘西卡’>‘大叶’>‘热研5号’>‘热研21号’。由此说明亲缘识别是柱花草与其邻近植物竞争和相互作用的重要机制,在生产中将亲缘关系相近的柱花草品种混播(如‘热研2号’、‘热研5号’和‘热研21号’柱花草混播)是发展牧草混播新技术、增加单位面积饲草产量的可能途径。

关键词：亲缘识别根系分泌物生物量分配根系生长植物竞争种内近亲种间远亲

数字金融、家庭财务脆弱性与返贫风险——基于中国家庭金融调查数据的实证检验

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南方金融 2023年第1期 37-49页

作者：李瑞晶王丽媛河北金融学院金融与投资学院河北保定071051

本文基于中国家庭金融调查数据,研究数字金融对家庭返贫风险的影响以及家庭财务脆弱性的非线性中介机制。研究结果表明:第一,数字金融对家庭返贫风险的影响呈U型特征,随着数字金融发展水平的提高,返贫风险先下降后上升。第二,数字金融... 详细信息

本文基于中国家庭金融调查数据,研究数字金融对家庭返贫风险的影响以及家庭财务脆弱性的非线性中介机制。研究结果表明:第一,数字金融对家庭返贫风险的影响呈U型特征,随着数字金融发展水平的提高,返贫风险先下降后上升。第二,数字金融防返贫非线性效应的中介机制是通过家庭财务脆弱性实现的,数字金融的适度运用有利于降低家庭财务脆弱性,防止家庭陷入贫困,但滥用会加剧家庭财务脆弱性,增加返贫风险。第三,金融素养较低、收入较低以及处于低市场化水平地区的家庭更容易遭受数字金融带来的负面影响。上述研究结论的政策启示:坚持统筹发展和安全,在推广应用数字金融的同时,注重防范金融风险;坚守数字金融的普惠本质,充分发挥数字金融对促进共同富裕的正向效应;加强金融消费者教育,提升居民的金融素养和抗风险能力。

关键词：共同富裕普惠金融数字金融家庭财务脆弱性返贫风险

Hepatitis B virus evades immune recognition via RNA adenosine deaminase ADAR1-mediated viral RNA editing in hepatocytes

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Cellular & Molecular Immunology 2021年第8期18卷 1871-1882页

作者： liyuan wang Yang Sun Xiaojia Song Zehua wang Yankun Zhang Ying Zhao Xueqi Peng Xiaodong Zhang Chunyang Li Chengjiang Gao Nailin Li Lifen Gao Xiaohong Liang Zhuanchang Wu Chunhong Ma Key Laboratory for Experimental Teratology Ministry of EducationSchool of Basic Medical SciencesCheeloo Medical CollegeShandong UniversityJinanShandongChina Department of Immunology School of Basic Medical SciencesCheeloo Medical CollegeShandong UniversityJinanShandongChina Key Laboratory of Infection and Immunity of Shandong Province Shandong UniversityJinanChina Advanced Medical Research Institute Shandong UniversityJinanChina Clinical Pharmacology Group Department of MedicineSolnaKarolinska InstituteStockholmSweden

HBV is considered as a“stealth”virus that does not invoke interferon(IFN)responses;however,the mechanisms by which HBV bypasses innate immune recognition are poorly *** this study,we identified adenosine deaminases acting on RNA 1(ADAR1),which is a key factor in HBV evasion from IFN responses in ***,ADAR1 interacted with HBV RNAs and deaminated adenosine(A)to generate inosine(I),which disrupted host immune recognition and thus promoted HBV *** of ADAR1 or its deficient deaminase activity promoted IFN responses and inhibited HBV replication in hepatocytes,and blocking the IFN signaling pathways released the inhibition of HBV replication caused by ADAR1 ***,the HBV X protein(HBx)transcriptionally promoted ADAR1 expression to increase the threshold required to trigger intrinsic immune activation,which in turn enhanced HBV escape from immune recognition,leading to persistent *** with 8-azaadenosine,an ADAR1 inhibitor,efficiently enhanced liver immune activation to promote HBV clearance in vivo and in *** together,our results delineate a molecular mechanism by which HBx promotes ADAR1-derived HBV immune escape and suggest a targeted therapeutic intervention for HBV infection.

关键词： ADAR1 RNA editing IFN response HBV replication HBx

Effect of Modified Biochar with Organic Fertiliser on the Growth and Development of Chinese Rose

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Advances in Bioscience and Biotechnology 2024年第6期15卷 344-359页

作者： liyuan Mu Hongyin Zhou Junlei wang Sijing Sun Haichan Yang Naiming Zhang Li Bao College of Resources and Environment Yunnan Agricultural University Kunming China Yunnan Engineering Research Centre for Soil Fertilisation and Pollution Remediation Kunming Yunnan School of Plant Protection Yunnan Agricultural University Kunming China

In order to reduce the waste of resources and environmental pollution caused by excessive application of chemical fertilizers, improve the utilization rate of fertilizers, and promote the large-scale and high-quality development of the Chinese rose industry. In this experiment, corn stover biochar, phosphoric acid modified biochar and organic fertilizer were used as test materials, and the effects of mixed application of modified biochar and organic fertilizer on the growth and development of Chinese rose as well as soil physicochemical properties were investigated by using the method of pot planting test. The results showed that modified biochar with organic fertilizer had the most significant effect on the enhancement of soil pH, organic matter content and soil carbon-to-nitrogen ratio. After 120 d of planting, modified biochar with organic fertilizer had the most significant effect on the enhancement of plant height and crown width of Chinese rose;both organic fertilizer and modified biochar with organic fertilizer significantly increased the chlorophyll content of Chinese rose. The number of flowers and the number of branches were the highest in the modified biochar with organic fertilizer treatment. In conclusion, the application of modified biochar with organic fertilizer can better improve the soil pH, and increase the soil organic matter content and carbon-to-nitrogen ratio to change the biological traits of Chinese rose. The results of this study provide a theoretical basis for the reduction of chemical fertilizers and the resource utilization of agricultural wastes and guarantee the sustainable development of the cut flower industry.

关键词： Biochar Modified Biochar Chinese Rose Organic Fertiliser