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基于CRISPR-dCas9转录激活系统的毛果杨ANT转录因子功能分析

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植物研究 2024年第3期44卷 431-440页

作者：孟令桐苏丽伟李祥欣熊天圣常攀鹏刘孟卓周晨光林木遗传育种全国重点实验室(东北林业大学) 哈尔滨150040 上海科技大学生命科学与技术学院上海201210

基于CRISPR-dCas9的基因转录激活表达能够避免基因异位表达带来的表型干扰,同时使基因有效地特异性表达。该研究利用新型CRISPR-Act3.0表达系统,在毛果杨(Populus trichocarpa)中对维管形成层特异表达转录因子ANT(AINTEGUMENTA)进行基... 详细信息

基于CRISPR-dCas9的基因转录激活表达能够避免基因异位表达带来的表型干扰,同时使基因有效地特异性表达。该研究利用新型CRISPR-Act3.0表达系统,在毛果杨(Populus trichocarpa)中对维管形成层特异表达转录因子ANT(AINTEGUMENTA)进行基因转录激活,创制遗传材料,并对基因的功能进行分析。首先对毛果杨PtrANTs转录因子进行同源分析,选取其中的PtrANT-4进行后续研究,对该基因进行克隆并利用荧光定量PCR分析其在各组织中的表达情况;其次在PtrANT-4启动子上设计3条gRNAs,构建CRISPR-dCas9转录激活表达载体,利用原生质体瞬时转化法检测该载体的表达;最后将该表达载体利用农杆菌介导法转化毛果杨,获得PtrANT-4转录激活遗传材料。结果表明:毛果杨中有4个PtrANTs转录因子,选取的PtrANT-4基因CDS序列全长为2 058 bp,编码685个氨基酸,在毛果杨侧生分生组织维管形成层特异表达。基于CRISPR-Act3.0表达系统成功构建的转录激活载体,在毛果杨木质部原生质体中转化后具有激活PtrANT-4表达的作用。获得的遗传转化植株中PtrANT-4基因仅在茎维管形成层中的表达量显著提高,说明PtrANT-4在茎维管形成层发育过程中可能起重要作用。该研究为PtrANT的功能研究奠定了一定研究基础,同时为维管形成层干细胞发育的机制研究提供了重要的遗传材料。

关键词： CRISPR-dCas9 基因激活表达 PtrANT 毛果杨

Cobalt-Organic Compound-Anchored Graphene Nanosheets as Efficient Lithium Storage Materials

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Cobalt-Organic Compound-Anchored Graphene Nanosheets as Effi...

第十四届全国有机电化学与工业学术会议暨中国化工学会精细化工专业委员会全国第182次学术会议

作者： liwei su 王连邦周震浙江工业大学化学工程学院南开大学化学学院

Lithium ion batteries(LIBs)are currently the dominant portable devices due to the good safety,high energy/power density,and environmental *** our recent work,CoCOcan in principle deliver a high capacity of 1350 mAh gbased on electrochemical catalytic conversion ***-oxidation carbon(C)in COcan be partially reduced to low-valence C under the electrochemical catalytic effect of the newly-generated Co nanoparticles,

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Mesoporous Mn3O4 nanoparticles/graphene nanocomposites as high-performance anode materials for lithium-ion batteries

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Mesoporous Mn3O4 nanoparticles/graphene nanocomposites as hi...

第一届全国储能科学与技术大会

作者： WU Xianbin su liwei WANG Lianbang College of Chemical Engineering Zhejiang University of Technology

To meet the ever-increasing demand for high energy/power anodes in lithium ion batteries(LIBs),considerable attention has been focused on transition metal oxides(MO,M=Fe,Co,Ni,Mn,etc)[1-3].MnO has a high theoretical capacity of 937 mA-h/g and a relatively-low electromotive force(1.032 V ***/Li)resulting in a lower voltage polarization[4].These features make it suitable as the next generation anode material for LIBs.

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Hydrothermal synthesis of xLi2MnO3·(1-x)LiMnO2 composites and its electrochemical performance by electrochemical pre-treatment

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Hydrothermal synthesis of xLi2MnO3·(1-x)LiMnO2 composites a...

第一届全国储能科学与技术大会

作者： JIANG Jingkang SHA Yali su liwei WANG Lianbang College of Chemical Engineering Zhejiang University of Technology

Over the past decades,lithium ion battery industry have been driven predominantly by the tremendous demand for electric vehicles(EVs)or hybrid electric vehicles(HEVs)[1-3].In the development of cathode materials,a solid solution system like xLiMnO-(1-x)LiMO(M=Ni,Mn,Co,Fe)has drawn much attention due to its extremely high capacity[4].In this system,LiMnO component is structurally integrated with layered LiMO,

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SiOx-PANI-Ag composites with homogeneously-embedded Si nanocrystals and nanopores as high-performance anodes for lithium ion batteries

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SiOx-PANI-Ag composites with homogeneously-embedded Si nanoc...

第一届全国储能科学与技术大会

作者： WANG Lianbang ZHANG Pinjie su liwei MA Chun’an College of Chemical Engineering Zhejiang University of Technology

The extremely large volume variation and poor electronic conductivity of Si anode materials for lithium ion batteries have seriously hampered their performances and practical applications[1-2].SiO materials are regarded as ideal alternatives to high-capacity Si anode materials for Li-ion batteries,since the generated LiO matrix from Li-SiO reactions can effectively accommodate the large

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Synthesis and electrochemical performance of ultrathin LiMnPO4/C nanosheets as cathode materials for Li ion batteries

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Synthesis and electrochemical performance of ultrathin LiMnP...

第一届全国储能科学与技术大会

作者： SHA Yali JIANG Jingkang su liwei WANG Lianbang College of Chemical Engineering Zhejiang University of Technology

Li-ion batteries(LIBs)are attracting more and more attentions in terms of high energy/power density,environmental benignity and good safety[1-2].Comparison with LiFePO,LiMnPO possesses a higher voltage plateau(4.1 V)and theoretical energy density,and hence is considered as a promising alternative[3].However,the low electronic conductivity(<10S/cm)and Liion conductivity(<10cm/s)seriously hamper its practical

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Discovery and structure-activity relationship studies of novel tetrahydro-β-carboline derivatives as apoptosis initiators for treating bacterial infections

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Journal of Integrative Agriculture 2024年第4期23卷 1259-1273页

作者： Shanshan su Hongwu Liu Junrong Zhang Puying Qi Yue Ding Ling Zhang Linli Yang liwei Liu Xiang Zhou Song Yang National Key Laboratory of Green Pesticide/Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education/Center for R&D of Fine ChemicalsGuizhou UniversityGuiyang 550025China

Developing and excavating new agrochemicals with highly active and safe is an important tactic for protecting crop health and food *** this paper,to discover the new bactericide candidates,we designed,prepared a new type of1,2,3,4-tetrahydro-β-carboline(THC)derivatives and evaluated the in vitro and in vivo bioactivities against the Xanthomonas oryzae ***(Xoo),Xanthomonas axonopodis ***(Xac),and Pseudomonas syringae ***(Psa).The in vitro bioassay results exhibited that most title molecules possessed good activity toward the three plant pathogenic bacteria,the compound A17 showed the most active against Xoo and Xac with EC50 values of 7.27 and 4.89 mg mL^(-1)respectively,and compound A8 exhibited the best inhibitory activity against Psa with EC50value of 4.87 mg mL^(-1).Pot experiments showed that compound A17 exhibited excellent in vivo antibacterial activities to manage rice bacterial leaf blight and citrus bacterial canker,with protective efficiencies of 52.67 and 79.79%at 200 mgmL^(-1),***,compound A8 showed good control efficiency(84.31%)against kiwifruit bacterial canker at 200 mg mL^(-1).Antibacterial mechanism suggested that these compounds could interfere with the balance of the redox system,damage the cell membrane,and induce the apoptosis of Xoo *** together,our study revealed that tetrahydro-β-carboline derivatives could be a promising candidate model for novel broadspectrum bactericides.

关键词： tetrahydro-β-carboline derivatives antibacterial activity ROS cell apoptosis

“计算机程序设计”课程中科学思维能力的培养

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计算机时代 2017年第1期 55-56,59页

作者：苏莉蔚吉林大学公共计算机教学与研究中心长春吉林130012

科学技术的进步使计算机在社会中的地位日益提高,应用范围越来越广,同时对大学生计算机水平的要求也越来越高。这给大学"计算机程序设计"课程提出了新的要求。课程要转变教学理念,改变教学方法,既要关注学生的文化知识学习,又要重视学... 详细信息

科学技术的进步使计算机在社会中的地位日益提高,应用范围越来越广,同时对大学生计算机水平的要求也越来越高。这给大学"计算机程序设计"课程提出了新的要求。课程要转变教学理念,改变教学方法,既要关注学生的文化知识学习,又要重视学生的能力培养。该课程要让学生学会提出问题、解决问题和总结问题,要在程序设计中培养学生的科学思维、进取精神和团结协作能力,以适应未来的发展。

关键词：计算机程序设计科学思维教学方法能力培养

SiOx-PANI-Ag Composites with Homogeneously-Embedded Si Nanocrystals and Nanopores as High-Performance Anodes for Lithium Ion Batteries

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SiOx-PANI-Ag Composites with Homogeneously-Embedded Si Nanoc...

第十四届全国有机电化学与工业学术会议暨中国化工学会精细化工专业委员会全国第182次学术会议

作者：王连邦 Pinjie Zhang liwei su Chun’an Ma 浙江工业大学化学工程学院

The extremely large volume variation and poor electronic conductivity of Si anode materials for lithium ion batteries have seriously hampered their performances and practical *** materials are regarded as ideal alternatives to high-capacity Si anode materials for Li-ion batteries,since the generated LiO matrix fiom Li-SiO reactions can effectively accommodate the large volume swing of ***,micron-sized particles with much smaller surface area

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