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Recent advances in nonlinear optics for bio‐imaging applications

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Opto-Electronic Advances 2020年第10期3卷 13-30页

作者： Silu Zhang liwei Liu Sheng Ren Zilin Li Yihua Zhao Zhigang Yang Rui Hu Junle qu Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518000China

Nonlinear optics,which is a subject for studying the interaction between intense light and materials,has great impact on various research *** many structures in biological tissues exhibit strong nonlinear optical effects,nonlinear optics has been widely applied in biomedical *** in the aspect of bio-imaging,nonlinear optical techniques can provide rapid,label-free and chemically specific imaging of biological samples,which enable the investigation of biological processes and analysis of samples beyond other microscopy *** this review,we focus on the introduction of nonlinear optical processes and their applications in bio-imaging as well as the recent advances in this *** perspective of this field is also presented.

关键词： nonliner optics microscopic imaging bio-imaging label-free chemical specific

山东烟区烤烟地上部临界氮稀释曲线的构建与营养诊断

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中国烟草科学 2024年第3期45卷 19-25页

作者：刘力玮刘洋王艺张杨吴强文曲远凯张玉臧传江闫慧峰中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室青岛266101 山东潍坊烟草有限公司山东潍坊261205 山东中烟工业有限责任公司济南250014

为建立山东烟区烤烟地上部临界氮稀释曲线,探讨以氮素营养指数诊断烤烟氮素营养状况的可行性,优化烤烟氮素管理,田间设置6个氮肥用量,分析其对移栽后烤烟地上部干物质积累量和氮浓度的影响,并通过烤烟氮营养指数判断烟株氮素营养的丰缺... 详细信息

为建立山东烟区烤烟地上部临界氮稀释曲线,探讨以氮素营养指数诊断烤烟氮素营养状况的可行性,优化烤烟氮素管理,田间设置6个氮肥用量,分析其对移栽后烤烟地上部干物质积累量和氮浓度的影响,并通过烤烟氮营养指数判断烟株氮素营养的丰缺情况。结果表明,随着施氮量增加,各取样时期烤烟干物质积累量均表现为先增加后稳定的趋势,不同施肥处理烤烟地上部干物质积累量差异显著;随着烤烟地上部干物质积累量的增加,不同施肥处理烤烟地上部氮浓度均逐渐降低;烤烟地上部临界氮浓度和干物质积累量符合幂函数关系,以地上部干物质积累量为驱动变量的临界氮稀释曲线为Nc=2.25×DM-0.13,其决定系数R2为0.72(p<0.05);不同氮肥处理氮营养指数(NNI)均在一定程度上呈现波动性,移栽后随时间增长呈现先上升后下降趋势,根据临界氮稀释曲线计算氮营养指数(NNI)判定烤烟氮营养状况,氮肥施用量90 kg/hm^(2)时氮营养状况较好。山东烟区烤烟地上部临界氮稀释曲线方程为Nc=2.25×DM-0.13,通过烤烟地上部临界氮稀释曲线与氮营养指数能够评价山东烟区烤烟氮营养状况,研究结果表明该试验地点适宜的氮肥施用量在90 kg/hm^(2)左右。

关键词：山东烟区临界氮浓度氮稀释曲线氮营养指数

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Atomic-resolution characterization on the structure of strontium doped barium titanate nanoparticles

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Nano Research 2021年第12期14卷 4802-4807页

作者： Haoyu Jiang Jizhen Qi Dongchang Wu Wei Lu Jiahui Qian Haifeng qu Yixiao Zhang Pei Liu Xi Liu liwei Chen In-situ Center for Physical Sciences School of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai200240China i-Lab CAS Center for Excellence in Nanoscience Suzhou Institute of Nano-Tech and Nano-Bionics(SINANO)Chinese Academy of SciencesSuzhou215123China Suzhou Bao Shun Mei Technology Co. Ltd.Room 305Building 1199 Jinji Lake AvenueSuzhou Industrial ParkSuzhou215123China DTU Nanolab Technical University of DenmarkFysikvejBuilding 3072800LyngbyDenmark Shanghai Nanoport Thermo Fisher Scientific Building A2517 Jinke RoadPudong DistrictShanghai201203China

Ferroelectric barium titanate nanoparticles(BTO NPs)may play critical roles in miniaturized passive electronic devices such as multi-layered ceramic *** increasing experimental and theoretical understandings on the structure of BTO and doped BTO have been developed over the past decade,the majority of the investigation was carried out in thin-film materials;therefore,the doping effect on nanoparticles remains ***,doping-induced local composition and structure fluctuation across single nanoparticles have yet to be *** this work,we use electron microscopy-based techniques including high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM),integrated differential phase contrast(iDPC)-STEM,and energy dispersive X-ray spectroscopy(EDX)mapping to reveal atomically resolved chemical and crystal structure of BTO and strontium doped BTO *** X-ray diffraction(PXRD)results indicate that the increasing strontium doping causes a structural transition from tetragonal to cubic phase,but the microscopic data validate substantial compositional and microstructural inhomogeneities in strontium doped BTO *** work provides new insights into the structure of doped BTO NPs and will facilitate the materials design for next-generation high-density nano-dielectric devices.

关键词： barium titanate nanoparticles doping effect structural transition high-angle annular dark-field scanning transmission electron microscopy integrated differential phase contrast

磨削术加超薄头皮移植术治疗烧伤后面部皮肤色素脱失的可行性及疗效

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武警医学 2018年第1期29卷 64-65,68页

作者：田晋洪赵俊丰杨淑云李涛张金辉窦丽伟蘧勤勤武警山西总队医院烧伤整形科太原030006 武警山西总队医院病理科太原030006

目的探讨磨削加超薄头皮移植术治疗面部烧伤后皮肤色素脱失的可行性及临床效果。方法 2013-03至2016-07采用局部磨削加超薄头皮移植术治疗面部烧伤皮肤色素脱失患者5例9处(其中住院患者2例,门诊患者3例,男3例,女2例)。色素脱失时间1~16... 详细信息

目的探讨磨削加超薄头皮移植术治疗面部烧伤后皮肤色素脱失的可行性及临床效果。方法 2013-03至2016-07采用局部磨削加超薄头皮移植术治疗面部烧伤皮肤色素脱失患者5例9处(其中住院患者2例,门诊患者3例,男3例,女2例)。色素脱失时间1~16年,色素脱失面积最大3 cm×4 cm,最小1 cm×0.8 cm。结果术后随访1~26个月,植皮成活良好,皮肤色素脱失部位明显改善,色泽、质地与周围皮肤接近,头皮供区愈合良好,毛发生长正常,患者满意。结论局部磨削加超薄头皮移植术,是治疗面部烧伤后皮肤色素脱失的有效方法,手术方法简单,效果确切,供区隐蔽,便于推广。

关键词：磨削术超薄皮片色素脱失烧伤

种植密度和氮肥用量对百色烟区烤烟叶片发育及烟叶产量的影响

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作物杂志 2024年第2期 189-197页

作者：解梦凡贾海江曲远凯农世英李俊霖王杰刘力玮闫慧峰中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室山东青岛266101 广西中烟工业有限责任公司广西南宁530001 广西壮族自治区烟草公司百色市公司广西百色533000

为明确不同种植密度和氮肥用量下烤烟的干物质积累、分配情况及烤烟中、上部叶的发育特征,以K326为试验材料,设置种植密度和氮肥用量两因素随机区组试验,分析影响烤烟群体和个体干物质积累的叶片发育特征。结果表明,种植密度和氮肥用量... 详细信息

为明确不同种植密度和氮肥用量下烤烟的干物质积累、分配情况及烤烟中、上部叶的发育特征,以K326为试验材料,设置种植密度和氮肥用量两因素随机区组试验,分析影响烤烟群体和个体干物质积累的叶片发育特征。结果表明,种植密度和氮肥用量对烤烟成熟期单株叶片干物质积累量均有极显著影响;成熟期烤烟单株叶片干物质积累量、整株干物质积累量、冠根比受种植密度和氮肥用量极显著交互效应的影响。施氮提高了烤烟中、上部叶现蕾期时的比叶重和叶片密度,种植密度对现蕾期和成熟期的中、上部叶单叶重及比叶重的影响均达到了极显著水平。烤烟叶片发育与干物质积累的相关分析表明,成熟期烤烟中部叶的比叶重和烤烟单株干物质积累量呈显著正相关,上部叶的叶片厚度与烤烟群体干物质积累量呈正相关。在低密度条件下,氮肥用量112.5 kg N/hm^(2)时烤烟上等烟比例达73.35%,在中等密度下,氮肥用量112.5 kg N/hm^(2)时烤烟产量、产值和均价达到各处理的最大值。综合考虑,在广西百色烟区周年烟稻轮作种植模式下,种植密度1.82万株/hm^(2)、基施氮肥用量112.5 kg N/hm^(2)处理能实现烤烟K326的最佳经济效益。

关键词：氮肥用量种植密度干物质积累量叶片发育特性产量

Implementation of FLIM and SIFT for improved intraoperative delineation of glioblastoma margin

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Chinese Optics Letters 2017年第9期15卷 20-24页

作者： Danying Lin Teng Luo liwei Liu Yuan Lu Shaoxiong Liu Zhen Yuan Junle qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University Shenzhen 518060 China Department of Dermatology The Sixth People＇s Hospital of Shenzhen Shenzhen 518052 China Department of Pathology The Sixth People＇s Hospital of Shenzhen Shenzhen 518052 China Bioimaging Core Faculty of Health Sciences University of Macao Taipa Maeau SAR China

The aim of this study is to develop a novel technique for improving the intraoperative margin assessment of glioblastoma by examining the total extrinsic extracellular matrix（ECM） with eosin staining using fluorescence lifetime imaging microscopy（FLIM） and scale-invariant feature transform（SIFT） descriptor analysis. Pseudocolor FLIM images obviously exhibit ECM distributions, changes in sequential sections, and different regions of interest. Meanwhile, SIFT descriptors are first utilized for the discrimination of glioblastoma margins by matching similar ECM regions and extracting keypoint orientations from FLIM images obtained from a series of continuous slices. The findings indicate that FLIM imaging with SIFT analysis of the total ECM is a promising method for improving intraoperative diagnosis of frozen and surgically excised brain specimen sections.

关键词： ECM Implementation of FLIM and SIFT for improved intraoperative delineation of glioblastoma margin

In vivo mice brain microcirculation monitoring based on contrast-enhanced SD-OCT

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Journal of Innovative Optical Health Sciences 2019年第1期12卷 62-71页

作者： Shaozhuang Yang liwei Liu Yixin Chang Ningning Zhang Kun Liu Liang Hong Bingling Chen Yue Zhao Rui Hu Junle qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060P.R.China State Key Laboratory of Supramolecular Structure and Materials Jilin University Changchun 130012P.R.China

In this paper,we proposed a contrast-enhanced homemade spectral domain optical coherence tomography(SD-OCT)method for monitoring of brain *** used the polyethylene glycol(PEG)-ylated gold nanorods(GNRs)as a contrast-enhanced agent,obtained clearly 2D and 3D OCT images of blood vessels and dynamic changes of probes in mouse blood *** to high scattering of the PEG-GNRs,more tiny blood vessels can be imaged and the OCT signal can be enhanced by 5.87 dB after injection of PEG-GNRs for 20 min,the enhancement then declined gradually for 60 *** results demonstrate an effective technique for the enhanced imaging of blood vessels in vivo,especially for studies of the brain microcirculation,which could be serviced for disease mechanism research and therapeutic drug monitoring.

关键词： Optical coherence tomography brain microcirculation cerebral blood vessels gold nanorods

Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei

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Synthetic and Systems Biotechnology 2023年第4期8卷 732-740页

作者： Qinqin Zhao Zezheng Yang Ziyang Xiao Zheng Zhang Jing Xing Huiqi Liang liwei Gao Jian Zhao Yinbo qu Guodong Liu State Key Laboratory of Microbial Technology Shandong UniversityQingdao266237China Taishan College Shandong UniversityQingdao266237China Tobacco Research Institute of Chinese Academy of Agricultural Sciences Qingdao266101China

The filamentous fungus Trichoderma reesei is widely used for the production of lignocellulolytic enzymes in ***1 is the major transcriptional activator of cellulases and hemicellulases in ***,rational engineering of XYR1 for improved lignocellulolytic enzymes production has been limited by the lack of structure ***,alanine 873 was identified as a new potential target for the engineering of XYR1 based on its structure predicted by *** mutation of this residue to tyrosine enabled significantly enhanced production of xylanolytic enzymes in the medium with cellulose as the carbon ***,xylanase and cellulase production increased by 56.7-and 3.3-fold,respectively,when glucose was used as the sole carbon *** both conditions,the improvements of lignocellulolytic enzyme production were higher than those in the previously reported V821F *** the enriched hemicellulases and cellulases,the crude enzymes secreted by the A873Y mutant strain produced 51%more glucose and 52%more xylose from pretreated corn stover than those of the parent *** results provide a novel strategy for engineering the lignocellulolytic enzyme-producing capacity of ***,and would be helpful for understanding the molecular mechanisms of XYR1 regulation.

关键词： Xylanase Cellulase Trichoderma reesei Transcription factor Genome editing

基于5G-CV2X的多路口智慧公交绿波通行方法

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沈阳大学学报（自然科学版） 2024年第4期36卷 331-340页

作者：马小陆徐立伟唐得志李国强齐晶晶瞿元安徽工业大学电气与信息工程学院安徽马鞍山243002 安徽工业大学芜湖技术创新研究院安徽马鞍山243002 安徽达尔智能控制系统股份有限公司安徽芜湖241002 奇瑞汽车股份有限公司安徽芜湖241006

提出了一种基于5G-CV2X的多路口智慧公交绿波通行方法。该方法通过5G-CV2X设备实时采集公交车行驶状态(速度、位置等)、公交车与当前路口的距离、路口位置和红绿灯的相位,云端使用基于遗传的非侵入式信号控制算法对多个路口红绿灯相位... 详细信息

提出了一种基于5G-CV2X的多路口智慧公交绿波通行方法。该方法通过5G-CV2X设备实时采集公交车行驶状态(速度、位置等)、公交车与当前路口的距离、路口位置和红绿灯的相位,云端使用基于遗传的非侵入式信号控制算法对多个路口红绿灯相位时长和公交车车速进行全局优化,然后云端将全局优化后的行车建议速度和指导相位通过5G发送至公交车和信号机,实现多路口绿波通行。实际道路测试验证,该方法能够实现多路口公交快速绿波通行,提升了公交系统的运行效率。

关键词：公交优先车路云协同 V2X 5G 遗传算法

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