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Antigens synthesis and antibodies preparation for furazolidone and its metabolite 3-amino-2-oxazolidinone

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Chinese Chemical Letters 2010年第9期21卷 1049-1052页

作者： Hua Ping Zhu Ting Ting liu Bing liu Hui ling Yin xiao Long Li Li Wang Shuo Wang Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Ministry of Education Tianjin 300457 China

Two haptens of 3-[（5-amino-furan-2-ylmethylene）amino]oxazolidin-5-one（FZ-NH_2） and 3-{[（4-carboxyphenyl）methylene]-amino} -2-oxazolidinone（CPAOZ） were *** FZ-NH2,immunogens were prepared by glutaraldehyde and diazo salt *** CPAOZ,immunogens were connected by the methods of the active ester and mixed acid *** with the combination,indirect competitive enzyme-linked immunosorbent assay（ic-ELISA） was developed with coating antigen of FZ-NH2 -OVA via the glutaraldehyde method and immunogen of CPAOZ-KLH via active ester *** furazolidone and its metabolite AOZ（NPAOZ as derivative）,the sensitivities（IC50） were 2.0μg/L and 2.5μg/L,limits of detection（IC15） were 0.09μg/L and 0.25μg/L,respectively.A sensitive method was developed for the simultaneous determination of furazolidone in feed and its metabolite AOZ in tissue.

关键词： Furzolidone AOZ Antigen Antibody ELISA

Interactions Between Effects of Estrogen Receptor Gene Polymorphisms on BMD and Experiences of the First Spermorrhea in Chinese Han Boys

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Biomedical and Environmental Sciences 2008年第2期21卷 129-136页

作者： JING YIN QIAN ZHANG xiao-QI HU WEI-JING DU AI-ling liu xiao-YAN WANG ZHI-QIN TONG GUAN-SHENG MA National Institute for Nutrition and Food Safety Chinese Center for Disease Control and Prevention 29 Nanwei Road Beijing 100050 China

Objective To study the interaction between polymorphisms of estrogen receptor （ER） gene and puberty on bone mineral density （BMD）. Methods One hundred and forty-six boys aged 13-17 years were divided into two groups according to their first sperrnorrhea. DNA was analyzed for Xba I and Pvu 1/genotypes by PCR-RFLP. BMD of the total body, forearm and lumbar spine was measured by dual-energy X-ray absorptiometry （DXA）. The relationship between polymorphisms of ER gene and BMD in these two groups was analyzed. Results The BMD at all sites in the sperrnorrhea group was significantly higher than that in the un-sperrnorrhea group. The independent contribution of ER genotypes to BMD at two pubertal stages was analyzed after adjusting co-variables. In the un-sperrnorrhea group, the BMD at distal 1/10 and 1/3 forearm of those carrying pp genotype was significantly higher than that of the non-carries, whereas in the sperrnorrhea group BMD in those carrying the same genotype was significantly lower than that in the non-carriers. Similar results were obtained by haplotype analysis. Multiple stepwise regression analysis showed that body weight, age and the first sperrnorrehea were the dominant determinants for BMD. BMD at forearm might be influenced by interaction between ER genotype and the first spermorrehea. Conclusion The polymorphisms of ER gene play a different role in BMD influenced by the first spermorrhea. Chinese boys carrying p or x allele should pay more attention to their bone mass.

关键词： BMD Puberty Hormones and receptors Polymorphism Boys

Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell-free extracts of Xenopus laevis eggs

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Cell Research 2000年第2期10卷 127-137页

作者： liu xiao ling YAN SHEN ER JIE CHEN ZHONG HEZHAI(E-mail: zhaizh@*** Tel: 86-10-62754293 Fax: 86-10-62751526)(College of Life Sciences, Peking University, Beijing 100871,China) College of Life Sciences Peking University Beijing China

Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation, nuclear envelope assembly, and nuclear *** Crythecodinium cohnii is a kind of primitive eukaryote which possesses numerous permanently condensed chromosomes and discontinuous double-layered nuclear membrane throughout the cell cycle. The assembled nuclei, being surrounded by a continuous double membrane containing nuclear pores and the uniformly dispersed chromatin fibers are morphologically distinguishable from that of Dinoflagellate Crythecodinium cohnii. However, incubation of dinoflagellate Crythecodinium cohnii chromosomes in the extracts from dinoflagellate Crythecodinillm cohnii cells does not induce nuclear reconstitution.

关键词： Dinoflagellate Crythecodinium cohnii chromosome nuclear reconstitution cell-free system Xenopus laevis egg extracts

Gingerol activates noxious cold ion channel TRPA1 in gastrointestinal tract

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Chinese Journal of Natural Medicines 2016年第6期14卷 434-440页

作者： YANG Meng-Qi YE Lin-Lan liu xiao-ling QI xiao-Ming LV Jia-Di WANG Gang FARHAN Ulah-Khan WAQAS Nawaz CHEN Ding-Ding HAN Lei ZHOU xiao-Hui Department of Clinical Pharmacy College of Pharmaceutical Sciences China Pharmaceutical University Department of Pharmacy Jiangsu Jiankang Vocational College

TRPA1 channels are non-selective cation channels that could be activated by plant-derived pungent products, including gingerol, a main active constituent of ginger. Ginger could improve the digestive function; however whether ginger improves the digestive function through activating TRPA1 receptor in gastrointestinal tract has not been investigated. In the present study, gingerol was used to stimulate cell lines(RIN14B or STC-1) while depletion of extracellular ***1 inhibitor(rethenium red) and TRPA1 gene silencing via TRPA1-specific si RNA were also used for mechanistic studies. The intracellular calcium and secretion of serotonin or cholecystokinin were measured by fura-2/AM and ELISA. Stimulation of those cells with gingerol increased intracellular calcium levels and the serotonin or cholecystokinin secretion. The gingerol-induced intracellular calcium increase and secretion(serotonin or cholecystokinin) release were completely blocked by ruthenium red, EGTA, and TRPA1-specific si RNA. In summary, our results suggested that gingerol derived from ginger might improve the digestive function through secretion releasing from endocrine cells of the gut by inducing TRPA1-mediated calcium influx.

关键词： Gingerol TRPA1 Intracellular calcium Serotonin Cholecystokinin

Co-integration Analysis on the Relationship between Methane Energy Development and Economic Growth in the City of Huixian

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Asian Agricultural Research 2011年第7期3卷 35-38页

作者： liu xiao-ling ZHENG Xun-gang Sichuan Agricultural University Chengdu 611130China

Based on the statistical data of Huixian from 1992 to 2010, we analyze the long-term and short-term relationship between Huixian's methane energy development and GDP by using co-integration test and error correction model. The empirical results show that there is a long-term equilibrium relationship between methane energy and GDP in the city of Huixian, and which is the one-way Granger causality of methane and GDP. In conclusion, the paper puts forward some steps about spurring economic growth, methane development and utilization in Huixian.

关键词： Methane energy Economic growth Co-integration analysis China

Chemerin deficiency regulates adipogenesis is depot different through TIMP1

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Genes & Diseases 2021年第5期8卷 698-708页

作者： Cheng-Long Huang liu-ling xiao Min Xu Jun Li Shu-Fen Li Cui-Song Zhu Yu-Li Lin Rui He Xi Li Biology Science Institutes Chongqing Medical UniversityChongqing400016PR China Center for Translational Research in Hematologic Malignancies Houston Methodist Cancer CenterHouston Methodist Research InstituteHoustonTX 77030USA Key Laboratory of Metabolic Molecular Medicine The Ministry of EducationDepartment of Biochemistry and Molecular BiologyFudan University Shanghai Medical CollegeShanghai200032PR China Shanghai Public Health Clinical Center Fudan UniversityShanghai201508PR China Department of Immunology Fudan University Shanghai Medical CollegeShanghai200032PR China

Adipocytes and immune cells are vital for the development of adipose ***-pokines secreted by adipocytes regulate adipogenesis and body *** is one of the ***,the function and mechanism of chemerin in adipose tissue are not fully *** with wild type(WT)mice,Rarres2^(-/-)mice gained weight and significantly increased fat distribution in subcutaneous adipose tissue(SAT),rather than visceral adipose tissue(VAT)on high fat diet(HFD).PPARg and C/EBPa,the master regulators of adipogenesis,were up-regulated in SAT and down-regulated in VAT in Rarres2^(-/-)mice comparing with WT *** of chemerin deficiency or not,the ratio of adipocyte-progenitors to total cells and the differentiation capacity of adipocyte-progenitors were similar in SAT and VAT,but macrophage infiltration in VAT was more severe than in SAT in Rarres2^(-/-)***,CD45þimmune cells supernatant from Rarres2^(-/-)SAT pro-moted the differentiation of adipocyte-progenitors and 3T3-L1 *** array assay of CD45þimmune cells supernatant revealed that metalloproteinase inhibitor 1(TIMP1),an in-hibitor of adipogenesis,was reduced in Rarres2^(-/-)SAT,but increased in Rarres2^(-/-)*** we specifically knocked down chemerin in SAT,TIMP1 was down-regulated and adipogenesis was promoted with reducing infiltration of *** present study demonstrates that the effects of chemerin on adipose tissue is depot different,and specific knock down chemerin in SAT promote adipogenesis and improve glucose tolerance test(GTT)and insulin tolerance test(ITT).This suggests a potential therapeutic target for chemerin in the treatment of obesity related metabolic disorder.

关键词： Adipogenesis Adipose tissue depot Chemerin Inflammation TIMP1

Computer-driven automated weaning reduces weaning duration in difficult-to-wean patients

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Chinese Medical Journal 2013年第10期126卷 1814-1818页

作者： liu ling XU xiao-ting YANG Yi HUANG Ying-zi liu Song-qiao QIU Hai-bo Department of Critical Care Medicine Nanjing Zhongda HospitalSoutheast University Nanjing Jiangsu 210-009 China

Background Weaning difficulties occur in 31% of total intubated patients, and result in prolonged weaning duration. A computer-driven automated weaning system can perform a spontaneous breathing trial （SBT） automatically and display a message when the trial is successfully passed. Such a system might have a beneficial effect on difficult-to-wean patients. The aim of this study was to examine whether the computer-driven automated weaning system can accelerate discontinuation of mechanical ventilation and improve outcomes in difficult-to-wean patients. Methods This randomized controlled study included 39 difficult-to-wean patients who failed their first spontaneous breathing trial. Before initiating weaning, eligible patients were randomly allocated to wean by computer-driven automated weaning system （CDW group, n=19） or a physician-controlled local protocol （PW group, n=20）. Weaning duration, defined as the time from inclusion until first extubation, was the primary endpoint. Secondary endpoints were total duration of mechanical ventilation, the length of intensive care unit （ICU） stay, the number of reintubations, the mortality rate in the ICU, the number of noninvasive ventilations, the number of complications in the ICU, and the number of ventilator-associated pneumonia cases. Results The weaning duration was reduced with the computer-driven weaning as compared with the usual protocol （median 29.0 hours vs. 45.5 hours, P=0.044）. Total duration of mechanical ventilation and duration of the ICU stay did not differ between the CDW and PW groups. There was no difference in the number of reintubations between the CDW and PW groups （3 and 4 patients, P=0.732）. The study groups showed comparable numbers of tracheostomy, self- extubations, ventilator-associated pneumonia, and non-invasive ventilation. Mortality in the ICU was similar in the CDW and the PW groups （21.1% vs. 20.0%, P=0.935）. Conclusion The computer-driven automated weaning system can reduce weaning duration

关键词： respiration artificial weaning computers airway extubation

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系统参数对全息法识别噪声源的影响

电子科技大学学报 2005年第4期34卷 541-544页

作者：刘晓玲张晓辉西华大学交通与汽车工程学院成都610039 西华大学科技处成都610039

通过由基尔霍夫边界条件推出的全息重建公式,介绍了近场声全息法进行噪声源识别的原理,并利用空间频域算法对声场进行重建,大大减少了重建过程的计算量,提高了全息重建的效率。同时,分析了计算及测试系统各主要参数对声源识别结果的影响... 详细信息

通过由基尔霍夫边界条件推出的全息重建公式,介绍了近场声全息法进行噪声源识别的原理,并利用空间频域算法对声场进行重建,大大减少了重建过程的计算量,提高了全息重建的效率。同时,分析了计算及测试系统各主要参数对声源识别结果的影响,对重建公式进行了验证,并开发出相应的计算机测试分析软件。结果表明:对重建公式进行修正并根据实际情况充分考虑各参数的影响后,声全息法可对噪声源进行有效识别,为采取噪声控制措施提供可靠依据。

关键词：声全息声源识别噪声控制近场全息

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科研组织运行的分析框架

科学学研究 2008年第3期26卷 563-568页

作者：刘小玲上海市科学学研究所上海200235

本文从科学社会学和组织社会学的视角,对科研组织的运行做了理论分析。认为科研组织的运行是研究目标、行为主体、认知方式、经费来源、运行规范、评价标准等六个结构性要素的相互作用的实践过程。

关键词：科研组织运行