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Chinese Chemical Letters 2006年第12期17卷 1555-1558页

作者： Jian Guo WANC Yong Jun XIAO Yong Hong LI Xing hai liu zheng Ming LI State-Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071

Based on the crystal structure of AHAS/sulfonylurea complex, we obtained 296 compounds with low binding energy towards AHAS via virtual screening. One series of them have been synthesized. Preliminary bioassay indicated that some compounds displayed good herbicidal activity on rape and bamygrass and inhibited AHAS to some extent. This study indicated the rationality of our molecular design based on the crystal structure of target enzyme.

关键词： AHAS molecular docking organic synthesis biological activity

Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect

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Military Medical Research 2023年第1期10卷 17-31页

作者： Piao Luo Qian Zhang Tian-Yu Zhong Jia-Yun Chen Jun-Zhe Zhang Ya Tian liu-hai zheng Fan Yang Ling-Yun Dai Chang Zou Zhi-Jie Li Jing-Hua liu Ji-Gang Wang Artemisinin Research Center and Institute of Chinese Materia MedicaChinese Academy of Chinese Medical SciencesBeijing 100700China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Laboratory Medicine the First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000JiangxiChina Department of Geriatric Medicine Shenzhen People’s Hospitalthe Second Clinical Medical CollegeJinan University and the First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020GuangdongChina Guangdong Provincial Key Laboratory of Proteomics School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Center for Reproductive Medicine Dongguan Maternal and Child Health Care HospitalSouthern Medical UniversityDongguan 523125GuangdongChina Central People’s Hospital of Zhanjiang Zhanjiang 524037GuangdongChina

Background: Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol(Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and ***: We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide(LPS). We screened for potential protein targets of Cel using activity-based protein profiling(ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays(CETSA) and surface plasmon resonance(SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene ***: Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS(P <0.05). Cel bound to Cys424 of pyruvate kinase M2(PKM2), inhibiting the enzyme and thereby suppressing aerobic glycolysis(Warburg effect). Cel also bound to Cys106 in high mobility group box 1(HMGB1) protein, reducing the secretion of inflammatory cytokine interleukin(IL)-1β. Cel bound to the Cys residues in lactate dehydrogenase A(LDHA).Conclusions: Cel inhibits inflammation and the Warburg effect in sepsis via targeting PKM2 and HMGB1 protein.

关键词： Celastrol Sepsis Pyruvate kinase M2 High mobility group box 1 Aerobic glycolysis

Effects of taurine and housing density on renal function in laying hens

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第12期17卷 952-964页

作者： Zi-li MA Yang GAO hai-tian MA liu-hai zheng Bin DAI Jin-feng MIAO Yuan-shu ZHANG College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 China Department of Orthopaedics Qilu Hospital Shandong University Jinan 250012 China Animal Husbandry and Veterinary Bureau of Dongyang City in Zhejiang Province Dongyang 322100 China

This study investigated the putative protective effects of supplemental 2-aminoethane sulfonic acid（taurine） and reduced housing density on renal function in laying hens. We randomly assigned fifteen thousand green-shell laying hens into three groups： a free range group, a low-density caged group, and a high-density caged group. Each group was further divided equally into a control group（C） and a taurine treatment group（T）. After 15 d, we analyzed histological changes in kidney cells, inflammatory mediator levels, oxidation and anti-oxidation levels. Experimental data revealed taurine supplementation, and rearing free range or in low-density housing can lessen morphological renal damage, inflammatory mediator levels, and oxidation levels and increase anti-oxidation levels. Our data demonstrate that taurine supplementation and a reduction in housing density can ameliorate renal impairment, increase productivity, enhance health, and promote welfare in laying hens.

关键词： Rearing pattern Welfare Taurine Laying hens Renal impairment

微偏振片阵列红外成像非均匀性产生机理及其校正

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光学精密工程 2018年第2期26卷 480-491页

作者：刘海峥史泽林冯斌中国科学院沈阳自动化研究所辽宁沈阳110016 中国科学院光电信息处理重点实验室辽宁沈阳110016 中国科学院大学北京100049

集成微偏振片阵列红外成像系统的偏振度图像对非均匀性高度敏感,不经非均匀校正的偏振度图像存在较大误差。为了校正微偏振片阵列红外成像的非均匀性,以入射光Stokes矢量形式,建立了光电转换基本过程的偏振像素模型,基于入射激励和辐射... 详细信息

集成微偏振片阵列红外成像系统的偏振度图像对非均匀性高度敏感,不经非均匀校正的偏振度图像存在较大误差。为了校正微偏振片阵列红外成像的非均匀性,以入射光Stokes矢量形式,建立了光电转换基本过程的偏振像素模型,基于入射激励和辐射响应数据,分析了微偏振阵列与红外焦面联合作用下非均匀性产生机理。提出一种基于多次辐射测量的矩阵形式的非均匀性校正方法,该方法通过构造多组测量方程,求解偏振像元的增益矢量,由相邻四像元增益矢量组成超级像元的增益矩阵,结合Stokes矢量提取矩阵,逆向求解重构点的校正矩阵。实验数据表明:该方法比两点法降低非均匀性约5%~20%,有效改善红外偏振度图像质量。

关键词：红外偏振成像微偏振片阵列非均匀性校正

Identification of antimalarial targets of chloroquine by a combined deconvolution strategy of ABPP and MS-CETSA

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Military Medical Research 2023年第1期10卷 64-77页

作者： Peng Gao Yan-Qing liu Wei Xiao Fei Xia Jia-Yun Chen Li-Wei Gu Fan Yang liu-hai zheng Jun-Zhe Zhang Qian Zhang Zhi-Jie Li Yu-Qing Meng Yong-Ping Zhu Huan Tang Qiao-Li Shi Qiu-Yan Guo Ying Zhang Cheng-Chao Xu Ling-Yun Dai Ji-Gang Wang Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Department of Traditional Chinese Medicine Southern Medical UniversityGuangzhou 510515China Department of Geriatrics the Second Clinical Medical College of Jinan Universitythe First Affiliated Hospital of Southern University of Science and TechnologyShenzhen People’s HospitalShenzhen 518020GuangdongChina Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)Singapore 138673Singapore Department of Biological Sciences National University of SingaporeSinga‑pore 117543Singapore

Background: Malaria is a devastating infectious disease that disproportionally threatens hundreds of millions of people in developing countries. In the history of anti-malaria campaign, chloroquine(CQ) has played an indispensable role, however, its mechanism of action(MoA) is not fully ***: We used the principle of photo-affinity labeling and click chemistry-based functionalization in the design of a CQ probe and developed a combined deconvolution strategy of activity-based protein profiling(ABPP) and mass spectrometry-coupled cellular thermal shift assay(MS-CETSA) that identified the protein targets of CQ in an unbiased manner in this study. The interactions between CQ and these identified potential protein hits were confirmed by biophysical and enzymatic ***: We developed a novel clickable, photo-affinity chloroquine analog probe(CQP) which retains the antimalarial activity in the nanomole range, and identified a total of 40 proteins that specifically interacted and photocrosslinked with CQP which was inhibited in the presence of excess CQ. Using MS-CETSA, we identified 83 candidate interacting proteins out of a total of 3375 measured parasite proteins. At the same time, we identified 8 proteins as the most potential hits which were commonly identified by both ***: We found that CQ could disrupt glycolysis and energy metabolism of malarial parasites through direct binding with some of the key enzymes, a new mechanism that is different from its well-known inhibitory effect of hemozoin formation. This is the first report of identifying CQ antimalarial targets by a parallel usage of labeled(ABPP)and label-free(MS-CETSA) methods.

关键词： Chloroquine Antimalaria Activity-based protein profiling(ABPP) Cellular thermal shift assay(CETSA) Quantitative proteomics

Influence of dietary taurine and housing density on oviduct function in laying hens

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 456-464页

作者： Bin DAI Yuan-shu ZHANG Zi-li MA liu-hai zheng Shuang-jie LI Xin-hong DOU Jian-sen GONG Jin-feng MIAO College of Veterinary Medicine Nanjing Agricultural University Animal Husbandry and Veterinary Bureau of Dongyang Poultry Institute Chinese Academy of Agricultural Sciences

Experiments were conducted to study the effects of dietary taurine and housing density on oviduct function in laying hens. Green-shell laying hens were randomly assigned to a free range group and two caged groups, one with low-density and the other with high-density housing. Each group was further divided into control（C） and taurine treatment（T） groups. All hens were fed the same basic diet except that the T groups＇ diet was supplemented with 0.1% taurine. The experiment lasted 15 d. Survival rates, laying rates, daily feed consumption, and daily weight gain were recorded. Histological changes, inflammatory mediator levels, and oxidation and anti-oxidation levels were determined. The results show that dietary taurine supplementation and reduced housing density significantly attenuated pathophysiological changes in the oviduct. Nuclear factor-κB（NF-κB） DNA binding activity increased significantly in the high-density housing group compared with the two other housing groups and was reduced by taurine supplementation. Tumor necrosis factor-α（TNF-α） m RNA expression in the high-density and low-density C and T groups increased significantly. In the free range and low-density groups, dietary taurine significantly reduced the expression of TNF-α m RNA. Supplementation with taurine decreased interferon-γ（IFN-γ） m RNA expression significantly in the low-density groups. Interleukin 4（IL-4） m RNA expression was significantly higher in caged hens. IL-10 m RNA expression was higher in the high-density C group than in the free range and low-density C groups. Supplementation with taurine decreased IL-10 m RNA expression significantly in the high-density group and increased superoxide dismutase（SOD） activity in the free range hens. We conclude that taurine has important protective effects against oviduct damage. Reducing housing density also results in less oxidative stress, less inflammatory cell infiltration, and lower levels of inflammatory mediators in the oviduct. Therefore, bo

关键词： Rearing pattern Taurine Laying hens Inflammation Oviduct injury

The crack tip fields of Mode Ⅱ stationary growth crack on bimaterial interface

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Journal of Marine Science and Application 2003年第2期2卷 11-16页

作者： TANG Li-qiang,liu Chang-hai, and zheng Gui School of Civil Engineering, Harbin Engineering University, Harbin 150001 , China School of Civil Engineering Harbin Engineering University Harbin China

A mechanical model is established for mode II interfacial crack static growing along an elastic-elastic power law creeping bimaterial interface. For frictional contact of boundary conditions on crack faces, asymptotic solutions of the stresses and strains of near tip-crack are got. It was shown that in stable creep growing phase, elastic deformation and viscous deformation are equally dominant in the near-tip field, the stress and strain have the same singularity and there is not the oscillatory singularity the field. Through numerical calculation , it is shown that the frictional coefficient η notably influence the crack-tip field.

关键词： an elastic-elastic power law creeping material near-tip field of mode Ⅱ interfacial crack the frictional effect

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基于曲波变换的边缘检测技术研究

南华大学学报（自然科学版） 2010年第1期24卷 52-55页

作者：刘征海南华大学计算机科学与技术学院湖南衡阳421001

曲波变换是一种新的多尺度表示方式,其系数包含位置和尺度信息.本文通过对曲波变换系数的分析,提出一种对各层系数区别处理的方式来加强图像边缘信息的方法.实验表明该方法能明显地加强灰暗部分物体的边缘.

关键词：边缘检测曲波变换 Canny算子

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一种MEMS陀螺仪的飞秒激光修调方法

中国惯性技术学报 2017年第2期25卷 260-264页

作者：刘宇航刘海平郑恒煜刘经勇陈志勇张嵘北京机械设备研究所北京100854 清华大学精密仪器系北京100084

受微加工工艺条件限制,MEMS敏感结构的尺寸等关键参数的相对误差较大,使其在宏观上表现出非理想的运动特性,性能指标也难以满足高精度应用的要求。为消除加工误差的影响,分析了一种MEMS陀螺仪的运动特性和误差耦合机理,提出了一种通过... 详细信息

受微加工工艺条件限制,MEMS敏感结构的尺寸等关键参数的相对误差较大,使其在宏观上表现出非理想的运动特性,性能指标也难以满足高精度应用的要求。为消除加工误差的影响,分析了一种MEMS陀螺仪的运动特性和误差耦合机理,提出了一种通过飞秒激光对敏感结构的梁进行刻蚀修调的方法。MEMS陀螺修调前后的运动特性试验表明,修调后的误差系数比修调前降低了50%以上,而误差的稳定性则比修调前提高了约70%,证明提出的飞秒激光刻蚀方法能够抑制微加工误差的影响,提高MEMS陀螺仪的性能。

关键词： MEMS陀螺仪飞秒激光零偏漂移结构修调

Comprehensive bibliometric analysis of pharmacotherapy for bipolar disorders:Present trends and future directions

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World Journal of Psychiatry 2025年第1期15卷 153-167页

作者： Bo-Fan Chen Li liu Fang-Zhen Lin hai-Min Zeng hai-Qiang Huang Chun-Fang Zhang Cong-Cong liu Xiang Chen Jie Peng Yun-Fa Wang Zhi-Lin Wang Bin Chen De-Le liu Yun liu zheng-zheng Li Xin-Xing Zeng The Second Affiliated Hospital Jiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina The Second Clinical Medical College Jiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina Department of Rehabilitation Medicine The Second Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Provincial People’s Hospital The First Affiliated Hospital of Nanchang Medical CollegeNanchang 330006Jiangxi ProvinceChina Department of Psychiatry Jiangxi Mental HospitalHospital of Nanchang UniversityNanchang UniversityNanchang 330029Jiangxi ProvinceChina

BACKGROUND Bipolar disorder(BD)is a severe mental illness characterized by significant mood *** drug treatment modalities are crucial for managing *** To analyze the current status and future trends of global research on BD drug treatment over the last *** The Web of Science Core Collection database spanning from 2015 to 2024 was utilized to retrieve literature related to BD drug treatment.A total of 2624 articles were *** visualization and analysis were conducted using CiteSpace,VOSviewer,Pajek,Scimago Graphica,and R-studio bibliometrix to identify RESULTS The United States,China,and the United Kingdom have made the most significant contributions to research on BD drug treatment and formed notable research collaboration *** University of Pittsburgh,Massachusetts General Hospital,and the University of Michigan have been identified as the major research institutions in this *** Journal of Affective Disorders is the most influential journal.A keyword analysis revealed research hotspots related to clinical symptoms,drug efficacy,and genetic mechanisms.A citation analysis identified the management guidelines published by Yatham et al in 2018 as the most cited *** This study provides a detailed overview of the field of BD drug treatment,highlighting key contributors,research hotspots,and future *** study findings can be employed as a reference for future research and policymaking,which may enable further development and optimization of BD pharmacotherapy.

关键词： Bipolar disorder Drug treatment Bibliometric analysis Visualization Mental disorder