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Effects of pyraclostrobin on growth,oxidative stress,and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

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Journal of Oceanology and Limnology 2024年第1期42卷 150-162页

作者： Yang liu Jiale ZHANG Peng XIAO Xin liu Yisifu MA jing ZHANG Bangjun ZHANG Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection Xinxiang 453007China College of Life Sciences Henan Normal UniversityXinxiang 453007China Journal of Henan Normal University Xinxiang 453007China College of Life and Environmental Science Wenzhou UniversityWenzhou 325035China Department of Ecosystem Studies School of Environmental ScienceUniversity of Shiga PrefectureShiga 522-8533Japan

Pyraclostrobin(PYR),a widely used fungicide,has negative effects on fish and algae,but its toxicity in protozoa remains *** this study,the effects of PYR on the growth,oxidative stress,and gene expression related to stress and ATP-binding cassette(ABC)transporters in Tetrahymena thermophila were *** result showed that the 96-h IC_(50)of PYR against *** was 17.2 mg/***,PYR inhibited the growth of *** in concentration-or time-dependent manner.A morphological study revealed that the shape and size of *** changed,and damage of cell membrane surface was observed by scanning electron microscopy after 96 h of PYR *** activities of superoxide dismutase(SOD)and catalase(CAT)increased throughout the *** contrast,the glutathione(GSH)content was increased at 24 h and 48 h of exposure and decreased at 96 ***,a significant increase in malondialdehyde(MDA)level was observed in *** after96 h of ***,PYR upregulated the HSP703,HSP705,GPx2,and ABAC15 gene expression in the 0.1–5-mg/L groups and downregulated the HSP704,HSP90,TGR,and ABCC52 mRNA levels at 96 h of *** results suggest that PYR may exert adverse effects on *** by inducing oxidative stress and changing the gene expression related to ABC transporters and stress,which may enrich the understanding of the toxicity mechanism of PYR in aquatic organisms and provide reference data for aquatic ecological risk assessments.

关键词： pyraclostrobin Tetrahymena thermophila growth oxidative stress gene expression

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Application of sparse S transform network with knowledge distillation in seismic attenuation delineation

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Petroleum Science 2024年第4期21卷 2345-2355页

作者： Nai-Hao liu Yu-Xin Zhang Yang Yang Rong-Chang liu jing-Huai Gao Nan Zhang School of Information and Communications Engineering Xi'an Jiaotong University School of Software Engineering Xi'an Jiaotong University PetroChina Research Institute of Petroleum Exploration and Development (RIPED) CNPC Research Institute of Exploration and Development Yumen Oilfield Company CNPC

Time-frequency analysis is a successfully used tool for analyzing the local features of seismic ***, it suffers from several inevitable limitations, such as the restricted time-frequency resolution,the difficulty in selecting parameters, and the low computational efficiency. Inspired by deep learning,we suggest a deep learning-based workflow for seismic time-frequency analysis. The sparse S transform network(SSTNet) is first built to map the relationship between synthetic traces and sparse S transform spectra, which can be easily pre-trained by using synthetic traces and training labels. Next, we introduce knowledge distillation(KD) based transfer learning to re-train SSTNet by using a field data set without training labels, which is named the sparse S transform network with knowledge distillation(KD-SSTNet).In this way, we can effectively calculate the sparse time-frequency spectra of field data and avoid the use of field training labels. To test the availability of the suggested KD-SSTNet, we apply it to field data to estimate seismic attenuation for reservoir characterization and make detailed comparisons with the traditional time-frequency analysis methods.

关键词： S transform Deep learning Knowledge distillation Transfer learning Seismic attenuation delineation

Investigation on photonic crystal nanobeam cavity based on mixed diamond–circular holes

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Nanotechnology and Precision Engineering 2024年第1期7卷 63-70页

作者： jingtong Bin Kerui Feng Shang Ma Ke liu Yong Cheng jing Chen Qifa liu College of Telecommunication and Information Engineering Nanjing University of Posts and TelecommunicationsNanjing 210003China College of Electronic and Optical Engineering and College of Flexible Electronics(Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210003China State Key Laboratory of Advanced Optical Communication Systems and Networks Shanghai Jiao Tong UniversityShanghai 200240China

A photonic crystal nanobeam cavity(M-PCNC)with a structure incorporating a mixture of diamond-shaped and circular air holes is *** performance of the cavity is simulated and studied *** theﬁnite-difference time-domain method,the parameters of the M-PCNC,including cavity thickness and width,lattice constant,and radii and numbers of holes,are optimized,with the quality factor Q and mode volume Vm as performance *** modulation of the lattice constant and hole radius enable the proposed M-PCNC to realize outstanding *** optimized cavity possesses a high quality factor Q 1.45105 and an ultra-small mode=×volume Vm 0.01(λ/n)[Zeng et al.,Opt Lett 2023:48;3981–3984]in the telecommunications wavelength *** can be progres-=sively squeezed in both the propagation direction and the perpendicular in-plane direction by a series of interlocked anti-slots and slots in the diamond-shaped hole ***,the energy can be conﬁned within a small mode volume to achieve an ultra-high Q/Vm ratio.

关键词： Photonic crystal nanobeam cavity Mixed diamond–circular holes Slots and anti-slots FDTD simulation Quality factor Mode volume

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Evolution of inclusions in vacuum induction melting of superalloys containing 70%return material

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Journal of Iron and Steel Research International 2024年第3期31卷 719-728页

作者： Jin-guo Gao Shu-lei Yang Shu-feng Yang jing-she Li Wei liu Meng-jing Zhao An-ren Wang School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Metallurgical and Building Materials Business Department China International Engineering Consulting CorporationBeijing 100048China

The variation law of inclusions type and size in the vacuum induction melting process and ingot of Ni-based superalloy containing 70%return material was studied by industrial test sampling,and the mechanism of inclusions formation was analyzed with thermodynamic *** results show that there are mainly two types of composite inclusions in the vacuum induction melting of Ni-based superalloys,which are nitride-and oxide-based composite inclusions,like Al_(2)O_(3)-SiO_(2)-Cr_(2)O_(3),TiN-(Mo,Nb)C,*** type and proportion of inclusions from the center to the edge of the vacuum induction ingot did not change *** number density of inclusions from the center to the edge of the ingot varied less,and the size of inclusions became smaller from the center to the *** addition,thermodynamic calculations show that oxides(M_(2)O_(3))are present in the liquid phase and mainly contain Al,Ti,Cr,Fe and O *** nitride consists mainly of Ti and N and contains small amounts of Cr,C,Nb,and Mo *** is consistent with the results of industrial *** the temperature decreases,the precipitation phases such as M_(2)O_(3),MN,γ,MC,δ,γ'andμphases are gradually precipitated,where oxides and nitrides are present in the liquid *** contents of O and N elements are the main influencing factors for the inclusions content and precipitation temperature;when the nitrogen content is reduced to below 0.0015%,it can make MN precipitate below the liquid-phase line.

关键词： Ni-based superalloy Vacuum induction melting Return material Inclusion Thermodynamic

Three dimensional velocity model and its tectonic implications at China Seismic Experimental Site,eastern margin of the Tibetan Plateau

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Science China Earth Sciences 2024年第7期67卷 2268-2290页

作者： Jianping WU Yan CAI Wei WANG Weilai WANG Changzai WANG Lihua faNG Yaning liu jing liu Institute of Geophysics China Earthquake AdministrationBeijing 100081China Beijing Baijiatuan Earth Science National Observation and Research Station Beijing 1095China Beijing Earthquake Agency Beijing 100080China

The China Seismic Experimental Site(CSES)is located at the intersection of the Tibetan Plateau,South China Block,and Indian Plate and has complex geological settings and intense crustal deformation,making it one of the most seismically active areas in Chinese mainland.A high-resolution,three-dimensional(3D)crust-mantle velocity structure is crucial for understanding seismotectonic environments,lithospheric deformation mechanisms,and deep dynamic *** first constructed a high-vertical-resolution 3D initial velocity model using the joint inversion of receiver functions and surface waves and then obtained a 3D P-and S-wave velocity model(CSES-VM1.0)with the highest lateral resolution of 0.25°for the CSES using double-difference *** to the limitations of the Sn observation data,the resolution of the S-wave velocity model in the lower crust and upper mantle was reduced,making it closer to the initial model provided by joint inversion.A comparison with explosive-source seismic data showed that the synthetic P-wave first-arrival travel times of the new model were closer to the observations than those of the previous velocity *** velocity cross-sections across the source areas of the 2022 Lushan MS6.1 and Ludian MS6.8 earthquakes reveal that the former earthquake occurred near a weak contact zone between the Tibetan Plateau and Sichuan Basin,and the rupture of the latter earthquake occurred in a granitic area,with the northern end blocked by rigid high-velocity bodies.A clear high-velocity anomaly zone is distributed along the western margin of the Yangtze Block,revealing the spatial distribution of Neoproterozoic intermediate-basic *** high-velocity zone significantly controls the morphology of fault zones and influences the rupture processes of major *** northeast-southwest and north-south trending high-velocity anomalies were found near Panzhihua,potentially related to Neoproterozoic and Middle-Late Permian intermediate

关键词： Eastern Tibetan Plateau China Seismic Experimental Site Yangtze Block Velocity model Double-difference tomography

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Climate change,ambient air pollution,and students'mental health

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World Journal of Psychiatry 2024年第2期14卷 204-209页

作者： jing-Xuan Wang Xin-Qiao liu School of Education Tianjin UniversityTianjin 300350China

The impact of global climate change and air pollution on mental health has become a crucial public health *** public awareness of health,advancements in medical diagnosis and treatment,the way media outlets report environmental changes and the variation in social resources affect psychological responses and adaptation methods to climate change and air *** the context of climate change,extreme weather events seriously disrupt people's living environments,and unstable educational environments lead to an increase in mental health issues for *** pollution affects students'mental health by increasing the incidence of diseases while decreasing contact with nature,leading to problems such as anxiety,depression,and decreased cognitive *** call for joint efforts to reduce pollutant emissions at the source,improve energy structures,strengthen environmental monitoring and governance,increase attention to the mental health issues of students,and help student groups build resilience;by establishing public policies,enhancing social support and adjusting lifestyles and habits,we can help students cope with the constantly changing environment and maintain a good level of mental *** these comprehensive measures,we can more effectively address the challenges of global climate change and air pollution and promote the achievement of the United Nations Sustainable Development Goals.

关键词： Climate change Ambient air pollution Mental health Energy structure Public policy Sustainable development

Chromosome-scale genomes of Quercus sichourensis and Quercus rex provide insights into the evolution and adaptation of fagaceae

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Journal of Genetics and Genomics 2024年第5期51卷 554-565页

作者： Xue liu Weixiong Zhang Yongting Zhang jing Yang Peng Zeng Zunzhe Tian Weibang Sun jing Cai Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection School of Ecology and EnvironmentNorthwestern Polytechnical UniversityXi'anShaanxi 710072China Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China

The fagaceae,a plant family with a wide distribution and diverse adaptability,has garnered significant interest as a subject of study in plant speciation and ***,certain fagaceae species are regarded as highly valuable wood resources due to the exceptional quality of their *** this study,we present two high-quality,chromosome-scale genome sequences for Quercus sichourensis(848.75 Mb)and Quercus rex(883.46 Mb).Comparative genomics analysis reveals that the difference in the number of plant disease resistance genes and the nonsynonymous and synonymous substitution ratio(Ka/Ks)of protein-coding genes among fagaceae species are related to different environmental ***,most genes related to starch synthesis in the investigated Quercoideae species are located on a single chromosome,as compared to the outgroup species,fagus ***,resequencing and population analysis of *** and *** reveal that *** has lower genetic diversity and higher deleterious mutations compared to *** high-quality,chromosome-level genomes and the population genomic analysis of the critically endangered *** and *** will provide an invaluable resource as well as insights for future study in these two species,even the genus Quercus,to facilitate their conservation.

关键词： Adaptation Quercus sichourensis Quercusrex Chromosome-scale genome assembly Tandem duplication genes Extremely small populations

CCaP1/CCaP2/CCaP3 interact with plasma membrane H^(+)-ATPases and promote thermoresponsive growth by regulating cell wall modification in Arabidopsis

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Plant Communications 2024年第7期5卷 90-105页

作者： jing-jing Wang Juan Gao Wei Li Jian-Xiang liu State Key Laboratory of Plant Environmental Resilience College of Life SciencesZhejiang UniversityHangzhou 310027China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310027China

Arabidopsis plants adapt to warm temperatures by promoting hypocotyl growth primarily through the basic helix-loop-helix transcription factor PIF4 and its downstream genes involved in auxin responses,which enhance cell *** the current study,we discovered that cell wall-related calcium-binding protein 2(CCaP2)and its paralogs CCaP1 and CCaP3 function as positive regulators of thermo-responsive hypocotyl growth by promoting cell elongation in ***,mutations in CCaP1/CCaP2/CCaP3 do not affect the expression of PIF4-regulated classic downstream ***,they do noticeably reduce the expression of xyloglucan endotransglucosylase/hydrolase genes,which are involved in cell wall *** also found that CCaP1/CCaP2/CCaP3 are predominantly localized to the plasma membrane,where they interact with the plasma membrane H^(+)-ATPases AHA1/***,we observed that vanadate-sensitive H^(+)-ATPase activity and cell wall pectin and hemicellulose contents are significantly increased in wild-type plants grown at warm temperatures compared with those grown at normal growth temperatures,but these changes are not evident in the ccap1-1 ccap2-1 ccap3-1 triple ***,our findings demonstrate that CCaP1/CCaP2/CCaP3 play an important role in controlling thermo-responsive hypocotyl growth and provide new insights into the alternative pathway regulating hypocotyl growth at warm temperatures through cell wall modification mediated by CCaP1/CCaP2/CCaP3.

关键词： AHA1/AHA2 CCaP1/CCaP2/CCaP3 cell wall modification hypocotyl growth thermomorphogenesis warm temperature

Robust zero-watermarking algorithm based on discrete wavelet transform and daisy descriptors for encrypted medical image

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CAAI Transactions on Intelligence Technology 2024年第1期9卷 40-53页

作者： Yiyi Yuan jingbing Li jing liu Uzair Aslam Bhatti Zilong liu Yen-wei Chen School of Information and Communication Engineering Hainan UniversityHaikouChina Research Center for Healthcare Data Science Zhejiang LabHangzhouChina Graduate School of Information Science and Engineering Ritsumeikan University ShigaJapan

In the intricate network environment,the secure transmission of medical images faces challenges such as information leakage and malicious tampering,significantly impacting the accuracy of disease diagnoses by medical *** address this problem,the authors propose a robust feature watermarking algorithm for encrypted medical images based on multi-stage discrete wavelet transform(DWT),Daisy descriptor,and discrete cosine transform(DCT).The algorithm initially encrypts the original medical image through DWT-DCT and Logistic ***,a 3-stage DWT transformation is applied to the encrypted medical image,with the centre point of the LL3 sub-band within its low-frequency component serving as the sampling *** Daisy descriptor matrix for this point is then ***,a DCT transformation is performed on the Daisy descriptor matrix,and the low-frequency portion is processed using the perceptual hashing algorithm to generate a 32-bit binary feature vector for the medical *** scheme utilises cryptographic knowledge and zero-watermarking technique to embed watermarks without modifying medical images and can extract the watermark from test images without the original image,which meets the basic re-quirements of medical image *** embedding and extraction of water-marks are accomplished in a mere 0.160 and 0.411s,respectively,with minimal computational *** results demonstrate the robustness of the algorithm against both conventional attacks and geometric attacks,with a notable performance in resisting rotation attacks.

关键词： daisy descriptor DCT DWT encryption domain medical image zero-watermarking