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World Journal of Gastroenterology 2007年第22期13卷 3133-3136页

作者： Hua liu Hui cao Zhi-Yong Wu Department of General Surgery Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 China

AIM： To develop a practical method for isolation, purification and culture of hepatic Kupffer cells （KCs） and to observe their suppressive effects on the proliferation of alloreactive T cells.METHODS： Perfusion in situ in vivo combined with density gradient centdfugation was applied in isolation, purification and culture of hepatic KC. The suppression by KCs on the T cell proliferation in mixed lymphocyte reaction （MLR） was observed.RESULTS：（1.1± 0.2） ×10^7 KCs per liver, （93.5% ±1.8%） viable cells, over 90% purity and positive for FD-2. After the first 24 h in culture, a great number of KCs which exhibited typical characteristics were observed. Using 3H-TdR incorporation assay, non-irradiated KCs significantly suppressed allo-MLR. The KCs recovered from accepted liver allografts in groups D and F were more effective in suppressing allo-MLR.CONCLUSION： A standardized procedure for isolation of highly purified rat KCs is proposed and KCs have suppressive effects on the proliferation of alloreactive T cells, especially those derived from accepted liver allografts.

关键词： Liver transplantation Kupffer cell T cell

Combined control of fluid adsorption capacity and initial permeability on coal permeability

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International Journal of Coal Science & Technology 2022年第6期9卷 127-137页

作者： Xiaolei liu Jianping Wei Guoying Wei Caifang Wu cao liu Xiaoming Ni State Key Laboratory Cultivation Base for Gas Geology and Gas Control Henan Polytechnic University454000 JiaozuoHenanChina College of Safety Science and Engineering Henan Polytechnic University454000 JiaozuoHenanChina State Collaborative Innovation Center of Coal Work Safety and Clean-efficiency Utilization 454000 JiaozuoHenanChina Key Laboratory of Coalbed Methane Resource and Reservoir Formation Process Ministry of EducationSouth Jiefang Road221008 XuzhouJiangsuChina Collaborative Innovation Center of Coalbed Methane(Shale Gas)in Central Plains Economic Zone 454000 JiaozuoChina

The variations of strain and permeability of coal were systematically studied through the physical simulation of N2 and water *** effects of fluid adsorption capacity and initial permeability on strain,permeability and the dominant effect of pore pressure were *** adsorption strain and strain rate of coal during water injection are significantly higher than those during N2 *** edge of free adsorption exists in the early phase of N2 and water injection,which is related to fluid *** this boundary,the strain rate and pore pressure are ***,the injec-tion time of initial stage accounts for about 20%of the total injection time,but the strain accounts for 70%of the total *** water injection,this boundary is about half of water saturation of ***,the influence of pore pressure on permeability is complex,which is controlled by adsorption capacity and initial permeability of *** the initial permeability is large enough,the effect of adsorption strain on permeability is relatively weak,and the promoting effect of pore pressure on fluid migration is ***,the permeability increases with increasing pore *** the initial permeability is relatively low,the pore pressure may have a dominant role in promoting fluid migration for the fluid with weak adsorption ***,for the fluid with strong adsorption capacity,the adsorption strain caused by pore pressure may play a leading role,and the permeability reduces first and then ascends with increasing pore pressure.

关键词： Initial permeability Adsorption capacity Free adsorption Pore pressure Coal strain

The Cell Modules of the Green Algebra of Drinfel'd Quantum Double D（H4）

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Acta Mathematica Sinica,English Series 2022年第6期38卷 1116-1132页

作者： liu Feng cao Hui Xiang CHEN Li Bin LI School of Mathematical Science Yangzhou University

This paper is devoted to studying the structures of the cell modules of the complexified Green algebra R(D（H4）),where D（H4） is the Drinfel'd quantum double of Sweedler's 4-dimensional Hopf algebra *** show that R(D（H4）) has one infinite dimensional cell module,one 4-dimensional cell module generated by all finite dimensional indecomposable projective modules of D（H4） and infinitely many 2-dimensional cell *** precisely,we obtain the decompositions of all finite dimensional cell modules into the direct sum of indecomposable submodules,and show that the infinite dimensional cell module can be written as the direct sum of two infinite dimensional indecomposable submodules.

关键词： Green algebra positively based algebra Drinfel’d quantum double cell module 13C13 16D80 16G30 16T05

Progress of Double Star Program

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空间科学学报 2006年第Z1期26卷 7-13页

作者： liu Zhenxing cao Jinbin Center for Space Science and Applied Research Chinese Academy of Sciences Beijing 100080 China Center for Space Science and Applied Research Chinese Academy of Sciences Beijing 100080 China

The Double Star Programme (DSP) is the first joint space mission between China and ESA. The mission, which is made of two spacecraft, is designed to investigate the magnetospheric global processes and their response to the interplanetary disturbances in conjunction with the Cluster mission. The first spacecraft, TC-1 (Tan Ce means "Explorer"), was launched on 30 December 2003, and the second one, TC-2, on 25 July 2004 on board two Chinese Long March 2C. Due to the importance of and success of DSP, both CNSA and ESA approved the extension of DSP. This paper presents DSP mission and some important scientific results made based on the data of DSP.

关键词： Double Star Program, Magnetosphere

Hydrodynamic performances and wakes induced by a generic submarine operating near the free surface in continuously stratified fluid

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水动力学研究与进展B辑:英文版 2023年第3期35卷 396-406页

作者： liu-shuai cao Gang Gao En-kai Guo De-cheng Wan School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

The wake of a submarine is a crucial element that greatly affects its stealth and hydrodynamic *** investigate the propagation and evolution characteristics of submarine wakes in a stratified fluid,particularly under free surface conditions,a numerical method to simulate the linearly stratified environment was developed.A thermocline model based on Boussinesq assumption was introduced to match the continuously stratified *** volume of fluid(VOF)method was utilized to capture free surface waves,while a fully structured grid and the SST k-ωturbulence model were combined to solve the complex flow of *** independence study in homogenous flow near the free surface was initially ***,the hydrodynamic performance,wake evolution characteristics,free surface signatures as well as decay of velocity under different stratification levels were *** demonstrated that the numerical method employed was efficient in simulating stratified ***,it showed that density stratification had a significant impact on the hydrodynamics and wake characteristics of a submarine,especially under strong density stratification circumstances.

关键词： Wakes submarine free surface stratified fluid hydrodynamics

A Brief Introduction and Recent Progress of the Geospace Double Star Program

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空间科学学报 2004年第Z1期24卷 39-45页

作者： liu Zhenxing cao Jinbin Center for Space Science and Applied Research The Chinese Academy of Sciences Beijing 100080 China Center for Space Science and Applied Research The Chinese Academy of Sciences Beijing 100080 China

The Geospace Double Star Project (DSP) consists of two small satellites operating in the near-earth equatorial and polar regions, respectively. The goals of DSP are: (1) to provide high-resolution field, particle, and wave measurements in some important near-earth active regions which have not been covered by current ISTP missions, such as the near-earth plasma sheet and its boundary layer, the ring current, the radiation belts, the dayside magnetopause boundary layer, and the polar region; (2) to investigate the trigger mechanisms of magnetic storms, magnetospheric substorms, and magnetospheric particle events,as well as the responses of geospace storms to solar activities and interplanetary disturbances; (3) to set up the models describing the spatial and temporal variations of the near-earth space *** realize the above goals, the equatorial satellite TC-1 and the polar satellite TC-2 will accommodate, respectively, eight instruments on board. TC-1was launched successfully in December 2003 while the polar satellite (TC-2)will be launched in July 2004. The orbit of the equatorial satellite TC-1 consists of a perigee at 550 km, an apogee at 60 000 km, and an inclination of about 28.5; while the orbit of the polar satellite will have a perigee of 700 km, an apogee of 40 000 km, and an inclination of about 90. The two satellites will take coordinated measurements with Cluster Ⅱ and will first form a "six-point exploration" in *** operational status of TC-1 are introduced in this paper.

关键词： Solar-terrestrial space physics, Double star exploration project,International cooperation

Capillary electrophoresis with electrochemiluminescence detection for the analysis of quinolone drugs and pharmacokinetics study

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Chinese Chemical Letters 2008年第8期19卷 962-964页

作者： Yan Ming liu Jun Tao cao Hui Wang College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 China

A novel method for the determination of two quinolone drugs norfloxacin （NOR） and levofloxacin （LVX） was described by capillary electrophoresis with electrochemiluminescence detection. The good relationship （r ≥ 0.9991） between peak area and concentration of analytes was established over two orders of magnitude. The limits of detection （LOD, S/N = 3） in standard solution are 4.8 × 10^-7 mol/L for NOR and 6.4 × 10^-7 mol/L for LVX, respectively. The limits of quantitation （LOQ, S/N = 10） in real human urine samples are 1.2 × 10^-6 mol/L for NOR and 1.4 × 10^-6 mol/L for LVX, respectively. The present method was successfully applied to the determination of NOR and LVX in human urine and the studv of oharmacokinetics of NOR.

关键词： Capillary electrophoresis Electrochemiluminescence detection Quinolone drugs Urine analysis Pharmacokinetics

Vortex tuning of a submarine by liutex force field model

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Journal of Hydrodynamics 2021年第3期33卷 503-509页

作者： liu-shuai cao Song-tao Chen De-cheng Wan Yi-qian Wang Computational Marine Hydrodynamics Lab(CMHL) School of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Ocean College Zhejiang UniversityZhoushan316021China School of Mathematical Science Soochow UniversitySuzhou215006China

Vortical structures of a submarine with appendages are fully turbulent and ***,flow control and vortex manipulation are of great importance for the hydrodynamic performance and acoustic *** the generic submarine model DARPA Suboff as the test case,a vortex tuning method based on the liutex force field is proposed to manipulate the vorticity *** flow past the submarine model in straight-line motion at a Reynolds number of 1.2×107 is achieved by solving the Reynolds averaged Navier-Stokes(RANS)***-block structured mesh topology is used to discretize the computational domain,and the shear stress transport(SST)k-ωturbulence model is implemented to close the *** control of vortex is achieved by introducing additional source terms based on liutex vortex definition and identification system to the RANS *** resistance acting on the submarine,flow field as well as the vortical structures are compared and *** show that liutex force model can effectively reduce the resistance by 9.31%and change the vortical structures apparently.

关键词： Vortex tuning vortical structures submarine vortex identification liutex force field model

Comparative analysis of impact of human occupancy on indoor microbiomes

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Frontiers of Environmental Science & Engineering 2021年第5期15卷 145-154页

作者： liu cao Lu Yang Clifford S.Swanson Shuai Li Qiang He Department of Civil and Environmental Engineering The University of TennesseeKnoxvilleTN37996USA Institute for a Secure and Sustainable Environment The University of TennesseeKnoxvilleTN37996USA

Educational facilities serve as community hubs and consequently hotspots for exposure to pathogenic ***,it is of critical importance to understand processes shaping the indoor microbiomes in educational facilities to protect public health by reducing potential exposure risks of students and the broader *** this study,the indoor surface bacterial microbiomes were characterized in two multifunctional university buildings with contrasting levels of human occupancy,of which one was recently constructed with minimal human occupancy while the other had been in full operation for six *** levels of human occupancy in the older building were shown to result in greater microbial abundance in the indoor environment and greater proportion of the indoor surface bacterial microbiomes contributed from human-associated microbiota,particularly the skin *** was further revealed that human-associated microbiota had greater influence on the indoor surface bacterial microbiomes in areas of high occupancy than areas of low *** with minimal impact from human occupancy in a new construction,the indoor microbiomes in the new building exhibited significantly lower influence from human-associated microbiota than in the older building,with microbial taxa originating from soil and plants representing the dominant constituents of the indoor surface bacterial *** contrast,microbial taxa in the older building with extensive human occupancy were represented by constituents of the human microbiota,likely from *** findings provide insights into processes shaping the indoor microbiomes which will aid the development of effective strategies to control microbial exposure risks of occupants in educational facilities.

关键词： Built environment Indoor microbiome Occupant Building Sequencing

Pt nanodendrites with a PtIr alloy surface structure exhibit excellent stability toward acidic hydrogen evolution reaction

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Nano Research 2024年第6期17卷 4844-4849页

作者： Chang liu Zongnan Wei Minna cao Rong cao State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China University of Chinese Academy of Sciences Beijing 100049China Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou 350108China

The development of effective and stable electrocatalysts for the hydrogen evolution reaction(HER)in acidic electrolytes is a significant *** this work,homogeneous Pt nanodendrites(Pt NDs)with a PtIr shell were successfully synthesized by a two-step wet chemical *** open three-dimensional(3D)dendritic structure exhibited exceptional electrocatalytic characteristics,exposing as many active sites as ***,by alloying Ir with Pt on the surface,catalytic activity was greatly enhanced while ensuring extremely high *** surface-enriched platinum nanodendritic catalysts(Pt@PtIr NDs)outperformed the control samples and the commercial *** acidic HER test,Pt@PtIr NDs had a lower overpotential(22 mV)than Pt NDs(26 mV)and commercial Pt/C(31 mV)at 10 mA/cm^(2),and the activity of Pt@PtIr NDs remained consistent even after undergoing a continuous durability test for at least 168 h,which was much superior to the performance of commercial Pt/C(10 h)under identical test *** study revealed that the application of 3D Pt dendritic metal alloys may offer a chance for the development of enhanced electrocatalysts in acidic HER.

关键词： Pt nanodendrites iridium high stability hydrogen evolution reaction alloy