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马蹄果冻的配方优化

食品安全导刊 2024年第23期 116-120页

作者：刘永吉刘国凌张池粼韶关学院食品学院广东韶关512005 韶关学院广东省粤北食药资源利用与保护重点实验室广东韶关512005

为研究马蹄粉在果冻中的应用效果,以马蹄粉、卡拉胶、果胶、木糖醇为主要原料,以感官评分为指标,进行单因素和正交试验优化马蹄果冻的配方。结果表明,卡拉胶和果胶复合胶添加量、马蹄粉添加量、木糖醇添加量对果冻感官有较大影响。马蹄... 详细信息

为研究马蹄粉在果冻中的应用效果,以马蹄粉、卡拉胶、果胶、木糖醇为主要原料,以感官评分为指标,进行单因素和正交试验优化马蹄果冻的配方。结果表明,卡拉胶和果胶复合胶添加量、马蹄粉添加量、木糖醇添加量对果冻感官有较大影响。马蹄果冻的最优配方为卡拉胶添加量1.8%,果胶添加量1.2%,马蹄粉添加量5%,木糖醇添加量20%,马蹄香精添加量0.06%。此时制作出来的果冻甜度适宜、口感光滑、组织状态均匀一致,具有马蹄独特香味。该配方下马蹄果冻的感官弹性为0.34、胶黏性为0.077 mJ、咀嚼性为0.57 mJ。

关键词：马蹄果冻淀粉配方

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响应面法优化皇帝柑精油提取工艺

韶关学院学报 2024年第3期45卷 1-5页

作者：刘国凌刘永吉陈江鹏韶关学院食品学院广东韶关512005

利用响应面法优化水蒸气蒸馏提取皇帝柑精油的提取工艺条件.探究提取时间、液料比、NaCl添加量、超声波功率对皇帝柑精油得率的影响,确定最佳工艺条件.结果表明,一种皇帝柑精油的最佳提取工艺为提取时间1.6 h、液料比32 mL·g^(-1)、NaC... 详细信息

利用响应面法优化水蒸气蒸馏提取皇帝柑精油的提取工艺条件.探究提取时间、液料比、NaCl添加量、超声波功率对皇帝柑精油得率的影响,确定最佳工艺条件.结果表明,一种皇帝柑精油的最佳提取工艺为提取时间1.6 h、液料比32 mL·g^(-1)、NaCl添加量1.8%,在此条件下皇帝柑精油得率为3.16%,与模型预测最大值3.19%相差无几,说明该提取工艺切实可行.

关键词：皇帝柑精油响应面法提取工艺优化

Analysis on The Incentive Effect of Occupational Pension in The Human Resource Strategy of Private University

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International Journal of Technology Management 2017年第1期 51-53页

作者： liu guoling Shaanxi Institute of International Trade&Commerce Shaanxi Xi'an 712046

Occupational pension can play an active role in the management of human resources at the present stage,and has the good *** a human resource strategy,the active application of occupational pension in private universities can effectively improve the traditional salary management work,promote the salary management work smoothly,and provide effective conditions for human resource *** private university human resources strategy,the positive application of occupational pension,need to continue to play its incentive function and effect,to attract more excellent teachers to participate in school construction,to improve the overall teaching *** paper mainly starts with the connotation and the definition of occupational pension,introduces and analyzes the relevant situation of occupational pension comprehensively,and gives a detailed description of the incentive effect of occupational pension in the human resource strategy of private universities.

关键词： Private University Human Resources Strategy Occupational Pension Incentive Effect

PbrARF4 contributes to calyx shedding of fruitlets in ‘Dangshan Suli’ pear by partly regulating the expression of abscission genes

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Horticultural Plant Journal 2024年第2期10卷 341-354页

作者： guoling Guo Pengfei Wei Tao Yu Haiyan Zhang Wei Heng Lun liu Liwu Zhu Bing Jia School of Horticulture Anhui Agricultural UniversityHefeiAnhui 230036China Huayuan Foreign Language School Zhejiang Normal UniversityJinhuaZhejiang 322100China Agricultural Experimental Center of Guiyang Guiyang Agriculture and Rural BureauGuiyangGuizhou 550018China

Fruitlet calyx shedding in pear plants is apparently regulated via numerous pathways that involve both environmental triggers and phytohormones cues such as auxin. In this study, we found at 10 days after full bloom (DAFB) higher levels of indoleacetic acid (IAA) and tryptophan (Trp) in calyx persistence fruitlet (CPF) than calyx shedding fruitlet (CSF) ofDanshan Suli’ pear (Pyrus bretschneideri Rhed.). Consisting with this, the activity of indolealdehyde oxidase (IAAIdO), which promotes IAA synthesis, was remarkably increased, and that of peroxidase(POD), which degrades IAA, dropped markedly in CPF but not in CSF. Further, qRT-PCR results revealed that most of 31 PbrARFs (encoding auxin response factors) in Pyrus bretschneideri were highly expressed in CPF, whereas PbrARF4, PbrARF24 and PbrARF26 were significantly downregulated in CPF vis-a-vis CSF. Phylogenetic analysis revealed that 6 PbrARFs clustered in the group III, where PbrARF4 showed the closest affinity with AtARF1 that promotes organ abscission, indicating a putative role of PbrARF4 in mediating the process of calyx shedding in pear. In fact, the ectopic overexpression of PbrARF4 in Solanum lycopersicum resulted in an earlier-formed and deeper abscission layer (AL) in the transgenic plants, whose calyxes were more prone to wilt at the mature red stage (MR) compared with the control plants (wild-type). More importantly, expression levels of the abscission genes SILS and Sl Cel2 in transgenic plants overexpressing PbrARF4 were significantly upregulated in comparation with the WT, whereas those of Sl BI and Sl TAPG2 were considerably inhibited. Further, PbrJOINTLESS and PbrIDA,the two genes related to calyx shedding in pear, were up-regulated more in CSF than CPF. The findings contribute to a better understanding of PbrARFs involved in fruitlet calyx shedding of pear, which could prove beneficial to improving the quality of pear fruit.

关键词： Pear Pyrus bretschneideri Rehd Calyx shedding IAA PbrARF4 Abscission genes

Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA_(3)-inhibited reactive oxygen species detoxification and abscisic acid signaling

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Journal of Integrative Agriculture 2024年第9期23卷 2989-3011页

作者： guoling Guo Haiyan Zhang Weiyu Dong Bo Xu Youyu Wang Qingchen Zhao Lun liu Xiaomei Tang Li liu Zhenfeng Ye Wei Heng Liwu Zhu Bing Jia School of Horticulture Anhui Agricultural UniversityHefei 230036China Anhui Wanbang Pharmaceutical Technology Co. Ltd.Hefei 230036China Science and Technology Department Anhui Agricultural UniversityHefei 230036China Hunan Suncas Agriculture Science Co. Ltd.Changsha 410016China

Drought stress is a devastating natural disaster driven by the continuing intensification of global warming,which seriously threatens the productivity and quality of several horticultural crops,including ***(GAs)play crucial roles in plant growth,development,and responses to drought *** studies have shown significant reductions of GA levels in plants under drought stress;however,our understanding of the intrinsic regulation mechanisms of GA-mediated drought stress in pear remains very ***,we show that drought stress can impair the accumulation of bioactive GAs(BGAs),and subsequently identified PbrGA2ox1 as a chloroplast-localized GA deactivation *** gene was significantly induced by drought stress and abscisic acid(ABA)treatment,but was suppressed by GA_(3)***2ox1-overexpressing transgenic tobacco plants(Nicotiana benthamiana)exhibited enhanced tolerance to dehydration and drought stresses,whereas knock-down of PbrGA2ox1 in pear(Pyrus betulaefolia)by virus-induced gene silencing led to elevated drought *** plants were hypersensitive to ABA,and had a lower BGAs content,enhanced reactive oxygen species(ROS)scavenging ability,and augmented ABA accumulation and signaling under drought stress compared to wild-type ***,the opposite effects were observed with PbrGA2ox1 silencing in ***,exogenous GA_(3)treatment aggravated the ROS toxic effect and restrained ABA synthesis and signaling,resulting in the compromised drought tolerance of *** summary,our results shed light on the mechanism by which BGAs are eliminated in pear leaves under drought stress,providing further insights into the mechanism regulating the effects of GA on the drought tolerance of plants.

关键词： pear gibberellin drought stress PbrGA2ox1 reactive oxygen species abscisic acid

城乡融合背景下长三角地区城乡人居空间变迁过程及特征

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地理科学进展 2023年第9期42卷 1745-1754页

作者：游猎刘国玲陈睿山上海交通大学设计学院上海200240

人口变化与用地增减的关系问题,是区域城乡发展面临的重要问题;城乡人地关系协调是城乡融合发展的客观要求。论文以长三角地区为例,运用人居空间变迁模型分析和比较了1997年以来苏浙沪皖三省一市,以及长三角地区市、县、镇、乡、村5级... 详细信息

人口变化与用地增减的关系问题,是区域城乡发展面临的重要问题;城乡人地关系协调是城乡融合发展的客观要求。论文以长三角地区为例,运用人居空间变迁模型分析和比较了1997年以来苏浙沪皖三省一市,以及长三角地区市、县、镇、乡、村5级行政单元人口变化与用地增减过程及特征。结果表明:(1)分省来看,苏浙皖三省整体呈现出城镇膨胀、蔓延与乡村萎缩、收缩互补态势;苏皖人地数量变化比沪浙更为协调,变迁强度更弱且更具稳定性。(2)分级来看,乡村地区人地数量变化一致性最弱,表现出明显的人口用地弹性,且异动明显;而市县镇变迁较为稳定。结论指出:人口要素虽然总体表现为乡村向城市逐级流动,但上海、浙江已经显示出人口的城乡双向流动;而用地要素则表现为乡村向城镇的单向流动;后者从要素间关系角度反映了“地随人走”的趋势。研究通过识别长三角地区城乡人居空间变迁过程及特征,有助于判断当前城乡融合的发展形势,并可为制定相关政策和评价标准提供参考。

关键词：城乡融合人地关系城乡关系人居空间变迁模型长三角地区

昆明山海棠对胶原性关节炎大鼠免疫功能的干预作用及其机制

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吉林大学学报（医学版） 2016年第1期42卷 64-69,I0003页

作者：母传贤刘国玲商丘医学高等专科学校外科教研室河南商丘476100 商丘医学高等专科学校生物化学与分子生物学教研室河南商丘476100

目的:观察昆明山海棠(THH)对胶原性关节炎(CIA)大鼠免疫功能的干预作用,探讨其可能的作用机制。方法:SD大鼠50只,随机分为正常组(n=10)和CIA模型制备组(n=40),用鸡Ⅱ型胶原(CⅡ)诱导大鼠制备CIA模型。再将造模成功的36只大鼠随机分为模... 详细信息

目的:观察昆明山海棠(THH)对胶原性关节炎(CIA)大鼠免疫功能的干预作用,探讨其可能的作用机制。方法:SD大鼠50只,随机分为正常组(n=10)和CIA模型制备组(n=40),用鸡Ⅱ型胶原(CⅡ)诱导大鼠制备CIA模型。再将造模成功的36只大鼠随机分为模型组、地塞米松组及200和400mg·kg-1 THH干预组。在实验过程中观察各组大鼠足爪肿胀度和关节炎指数(AI)等变化。实验第35天,采用ELISA法检测血清中抗CⅡ抗体水平;MTT法检测脾T细胞和B细胞增殖活性;ELSIA法检测脾组织白细胞介素12(IL-12)、IL-23和肿瘤坏死因子α(TNF-α)水平;取各组大鼠足爪组织行HE染色,观察组织病理学变化。结果:与正常组比较,模型组大鼠AI明显上升(P0.05)。结论:高剂量(400mg·kg-1)THH对CIA大鼠关节炎有明显抑制作用,其机制可能是THH能明显抑制T和B淋巴细胞的增殖反应,并抑制脾组织中IL-23、TNF-α和IL-12和血清中抗CⅡ抗体水平,进而明显改善大鼠足爪组织病理学变化。

关键词：昆明山海棠胶原性关节炎免疫功能

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水果综合分选机称重模块的设计与实现

农业工程学报 2009年第2期25卷 96-100页

作者：李光梅魏新华李法德颜世涛刘果玲山东农业大学机械与电子工程学院泰安271018 江苏大学农业工程研究院镇江212013 山东交通职业学院泰山校区泰安271018

为满足水果外观品质和质量综合分选的实际需要,设计了水果机器视觉质量综合分选机的称重模块。通过受力分析得出了水果质量的理论计算公式,给出了水果同步称重过程的实现方法。通过对质量不同的苹果进行实测标定,得出了等效质量w与其A/... 详细信息

为满足水果外观品质和质量综合分选的实际需要,设计了水果机器视觉质量综合分选机的称重模块。通过受力分析得出了水果质量的理论计算公式,给出了水果同步称重过程的实现方法。通过对质量不同的苹果进行实测标定,得出了等效质量w与其A/D转换值之间的关系式,并进行了试验验证。试验结果表明,所设计的称重模块可以与水果机器视觉分选机配合,对水果进行外观品质和质量的检测,称重精度约为2%。

关键词：机器视觉称重模块同步控制水果综合分选机

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油气藏流体样品的取样浅析

油气井测试 2006年第2期15卷 69-70页

作者：刘玲玲孟庆波刘国玲梁培文中原油田分公司采油一厂河南濮阳457001 中油测井公司北京100043 中原油田分公司采油二厂河南范县457532

油气藏流体取样对于设计油气藏开发工艺方案有着极其重要的作用。针对油气藏流体取样作业过程中的取样时机及不同井况的取样井所采用的技术调整措施进行了简要分析。

关键词：油气藏流体泡点压力露点压力