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Journal of Integrative Plant Biology 2005年第9期47卷 1107-1114页

作者： Mao-Sen liu fang wang Yu-Xiu DONG Xian-Sheng ZHANG Shandong Key Laboratory of Crop Biology College of Life Sciences Shandong Agricultural University Taian Shandong 271018 China

The grain color of wheat （Triticum aestivum L.） is an important characteristic in crop production. Dihydroflavonol 4-reductase genes （DFR） encode the key enzyme dihydroflavonol 4-reductase, which is involved in the pigmentation of plant tissues. To investigate the molecular mechanism of anthocyanin deposition in grains of wheat, we determined the expression of the wheat DFR gene in purple grains of cultivar Heimai 76. The results showed that DFR transcripts were localized in the seed coat of purple grains rather than in the pericarp, whereas anthocyanins were accumulated in both tissues of purple grains, suggesting that anthocyanin deposition was mainly regulated at the transcriptional level. Overexpression of the TaDFR-A gene in Arabidopsis showed that TaDFR-A was responsible for the pigmentation of Arabidopsis plant tissues, indicating TaDFR-A gene has the same role in Arabidopsis.

关键词： anthocyanin dihydroflavonol 4-reductase localization of TaDFR transcripts purple grain wheat.

Thermo-hydro-mechanical (THM) coupled simulation of the land subsidence due to aquifer thermal energy storage (ATES) system in soft soils

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第6期16卷 1952-1966页

作者： Yang wang Fengshou Zhang fang liu Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai200092China Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education Tongji UniversityShanghai200092China Shanghai Engineering and Technology Research Center of Shallow Geothermal Energy Shanghai200072China

Aquifer thermal energy storage(ATES)system has received attention for heating or cooling ***,it is well known that land subsidence becomes a major environmental concern for ATES ***,the effect of temperature on land subsidence has received practically no attention in the *** paper presents a thermo-hydro-mechanical(THM)coupled numerical study on an ATES system in Shanghai,*** water wells were installed for seasonal heating and cooling of an agriculture *** target aquifer at a depth of 74e104.5 m consisted of alternating layers of sand and silty sand and was covered with *** level,temperature,and land subsidence data from 2015 to 2017 were collected using field monitoring *** by data,we constructed a field scale three-dimensional(3D)model using TOUGH(Transport of Unsaturated Groundwater and Heat)and FLAC3D(Fast Lagrangian Analysis of Continua)equipped with a thermo-elastoplastic constitutive *** effectiveness of the numerical model was validated by field *** model was used to reproduce groundwater flow,heat transfer,and mechanical responses in porous media over three years and capture the thermo-and pressure-induced land *** results show that the maximum thermoinduced land subsidence accounts for about 60%of the total *** thermo-induced subsidence is slightly greater in winter than that in summer,and more pronounced near the cold well area than the hot well *** study provides some valuable guidelines for controlling land subsidence caused by ATES systems installed in soft soils.

关键词： Aquifer thermal energy storage(ATES) Land subsidence TOUGH-FLAC3D Thermo-elastoplastic constitutive model

Lorentz violation induces isospectrality breaking in Einstein-bumblebee gravity theory

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 87-96页

作者： Wentao liu Xiongjun fang Jiliang Jing Jieci wang Department of Physics Key Laboratory of Low Dimensional Quantum Structures and Quantum Control of Ministry of EducationSynergetic Innovation Center for Quantum Effects and ApplicationsHunan Normal UniversityChangsha 410081China

In this study,we investigate the quasinormal modes(QNMs)of a Lorentz-violating spacetime,factoring in a cosmological constant,within the framework of Einstein-bumblebee *** findings reveal that the interaction of spacetime with an anisotropic bumblebee field imparts distinct contributions to the axial and polar sectors of the vector *** subsequently breaks the isospectrality typically observed in vector *** evidence strongly indicates isospectral breaking in the vector modes of Einstein-bumblebee black holes:a pronounced breakage in the real part of the frequencies,while the imaginary component seems less *** isospectral breaking indicates the existence of two different waveforms in the Ringdown phase of the black hole,which provides a potential signal of quantum gravity observable in current experiments.

关键词： Lorentz-violating isospectral breaking quasinormal modes

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Influence of Pin Offset on Microstructure and Mechanical Properties of Friction Stir Welded Mg/Ti Dissimilar Alloys

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Acta Metallurgica Sinica(English Letters) 2019年第10期32卷 1261-1268页

作者： Qi Song Zhong-Wei Ma Shu-De Ji Qing-Hua Li liu-fang wang Rui Li School of Aerospace Engineering Shenyang Aerospace University

The influences of pin offset on the formation, microstructure and mechanical properties of friction stir welded joint of Ti6 Al4 V and AZ31 B Mg dissimilar alloys were investigated. The results show that sound joints are obtained at different offsets. With the offset decreasing from 2.5 to 2.1 mm, the number of Ti alloy fragments is increased, and the stir zone(SZ) is enlarged and the grains in SZ become coarser. A hook-like structure is formed at the Mg/Ti interface and its length is increased with the decrease in pin offset. The Al element has an enrichment trend at the Ti alloy side near the Mg/Ti interface when the offset is decreased, which is beneficial to the bonding of the interface. An Al-rich layer with a thickness of 3–5μm forms at the offset of 2.1 mm. All the joints fracture at the interface and present a mixed ductile-and-brittle fracture mode. The joint tensile strength is increased with the offset decreasing from 2.5 to 2.1 mm, and the maximum tensile strength of 175 MPa is acquired at the offset of 2.1 mm.

关键词： Mg/Ti dissimilar alloys Friction stir welding Pin offset Mechanical properties Microstructure

Three dimensional velocity model and its tectonic implications at China Seismic Experimental Site,eastern margin of the Tibetan Plateau

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Science China Earth Sciences 2024年第7期67卷 2268-2290页

作者： Jianping WU Yan CAI Wei wang Weilai wang Changzai wang Lihua fang Yaning liu Jing liu Institute of Geophysics China Earthquake AdministrationBeijing 100081China Beijing Baijiatuan Earth Science National Observation and Research Station Beijing 1095China Beijing Earthquake Agency Beijing 100080China

The China Seismic Experimental Site(CSES)is located at the intersection of the Tibetan Plateau,South China Block,and Indian Plate and has complex geological settings and intense crustal deformation,making it one of the most seismically active areas in Chinese mainland.A high-resolution,three-dimensional(3D)crust-mantle velocity structure is crucial for understanding seismotectonic environments,lithospheric deformation mechanisms,and deep dynamic *** first constructed a high-vertical-resolution 3D initial velocity model using the joint inversion of receiver functions and surface waves and then obtained a 3D P-and S-wave velocity model(CSES-VM1.0)with the highest lateral resolution of 0.25°for the CSES using double-difference *** to the limitations of the Sn observation data,the resolution of the S-wave velocity model in the lower crust and upper mantle was reduced,making it closer to the initial model provided by joint inversion.A comparison with explosive-source seismic data showed that the synthetic P-wave first-arrival travel times of the new model were closer to the observations than those of the previous velocity *** velocity cross-sections across the source areas of the 2022 Lushan MS6.1 and Ludian MS6.8 earthquakes reveal that the former earthquake occurred near a weak contact zone between the Tibetan Plateau and Sichuan Basin,and the rupture of the latter earthquake occurred in a granitic area,with the northern end blocked by rigid high-velocity bodies.A clear high-velocity anomaly zone is distributed along the western margin of the Yangtze Block,revealing the spatial distribution of Neoproterozoic intermediate-basic *** high-velocity zone significantly controls the morphology of fault zones and influences the rupture processes of major *** northeast-southwest and north-south trending high-velocity anomalies were found near Panzhihua,potentially related to Neoproterozoic and Middle-Late Permian intermediate

关键词： Eastern Tibetan Plateau China Seismic Experimental Site Yangtze Block Velocity model Double-difference tomography

A Dietary Source of High Level of Fluoroquinolone Tolerance in mcr-Carrying Gram-Negative Bacteria

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Research 2024年第3期2023卷 105-121页

作者： Tianqi Xu Dan fang Fulei Li Zhiqiang wang Yuan liu Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses College of Veterinary MedicineYangzhou UniversityYangzhou 225009China. Joint International Research Laboratory of Agriculture and Agri-Product Safety the Ministry of Education of ChinaYangzhou UniversityYangzhou 225009China. Institute of Comparative Medicine Yangzhou UniversityYangzhou 225009China.

The emergence of antibiotic tolerance,characterized by the prolonged survival of bacteria following antibiotic exposure,in natural bacterial populations,especially in pathogens carrying antibiotic resistance genes,has been an increasing threat to public ***,the major causes contributing to the formation of antibiotic tolerance and underlying molecular mechanisms are yet poorly ***,we show that potassium sorbate(PS),a widely used food additive,triggers a high level of fluoroquinolone tolerance in bacteria carrying mobile colistin resistance gene *** studies demonstrate that PS treatment results in the accumulation of intracellular fumarate,which activates bacterial two-component system and decreases the expression level of outer membrane protein OmpF,thereby reducing the uptake of *** addition,the supplementation of PS inhibits aerobic respiration,reduces reactive oxygen species production and alleviates DNA damage caused by bactericidal ***,we demonstrate that succinate,an intermediate product of the tricarboxylic acid cycle,overcomes PS-mediated ciprofloxacin *** multiple animal models,ciprofloxacin treatment displays failure outcomes in PS preadministrated animals,including comparable survival and bacterial loads with the vehicle *** together,our works offer novel mechanistic insights into the development of antibiotic tolerance and uncover potential risks associated with PS use.

关键词： overcome thereby additive

Flame Morphology and Characteristic of Co-Firing Ammonia with Pulverized Coal on a Flat Flame Burner

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Journal of Thermal Science 2024年第5期33卷 1935-1945页

作者： wang Shengye CUI Mingshuang liu Pengzhong DI Yi NIU fang China Coal Research Institute Beijing 100013China Beijing Tiandi Rongchuang Technology Co.Ltd. Beijing 100013China National Energy Technology&Equipment Laboratory of Coal Utilization and Emission Control Beijing 100013China

Ammonia as a new green carbon free fuel co-combustion with coal can effectively reduce CO_(2)emission,but the research of flame morphology and characteristics of ammonia-coal co-combustion are not *** this work,we studied the co-combustion flame of NH_(3)and pulverized coal on flat flame burner under different oxygen mole fraction(X_(i,O_(2)))and NH_(3)co-firing energy ratios(E_(NH_(3))).We initially observed that the introduction of ammonia resulted in stratification within the ammonia-coal co-combustion flame,featuring a transparent flame at the root identified as the ammonia combustion *** to challenges in visually observing the ignition of coal particles in the ammonia-coal co-combustion flame,we utilized Matlab software to analyze flame images across varying E_(NH_(3))and X_(i,O_(2)).The analysis indicates that,compared to pure coal combustion,the addition of ammonia advances the ignition delay time by 4.21 ms to 5.94 *** E_(NH_(3))increases,the ignition delay time initially decreases and then ***,an increase in X_(i,O_(2))results in an earlier ignition delay *** burn-off time and the flame divergence angle of pulverized coal demonstrated linear decreases and increases,respectively,with the growing ammonia *** addition of ammonia facilitates the release of volatile matter from coal ***,in high-ammonia environments,oxygen consumption also impedes the surface reaction of coal ***,measurements of gas composition in the ammonia-coal flame flow field unveiled that the generated water-rich atmosphere intensified coal particle gasification,resulting in an elevated concentration of ***,nitrogen-containing substances and coke produced during coal particle gasification underwent reduction reactions with NO_(x),leading to reduced NO_(x)emissions.

关键词： co-firing ammonia with coal flame characteristic gas component ignition delay time burn-off time flame divergence angle flat flame burner flame morphology

Characteristics of atmospheric nitrous oxide observed at *** GAW global station in the inland Eurasia during eighteen years

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Science China Earth Sciences 2024年第1期67卷 92-104页

作者： Miao LIANG Shuangxi fang Lixin liu Yong ZHANG Jianqiong wang Shuo liu Hongyang wang Liangchun DENG Meteorological Observation Centre(MOC) China Meteorological Administration(CMA)Beijing 100081China College of Environment Zhejiang University of TechnologyHangzhou 310014China Mt.Waliguan Background Station China Meteorological Administration(CMA)Xining 810001China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Center for Environmental Progress Wuhan 430070China

This study presents atmospheric N_(2)O mole fractions measured from discrete air samples from 2001 to 2018 at ***(WLG)station(36°17′N,100°54′E,3816 m asl)in China,which is a global background station of the World Meteorological Organization/Global Atmosphere Watch Programme(WMO/GAW)in central *** N_(2)O characteristics of annual means,interannual variability,and seasonal cycles were *** results show that N_(2)O at WLG possess a distinct increasing trend and a statistically significant seasonal cycle,with an average growth rate of 0.9±0.01 ppb yr^(−1)(1σ)(1ppb=10^(−9)),which is close to the global *** detrended seasonal cycle shows a trough of−0.25±0.04(1σ)ppb in June and a peak of 0.13±0.07(1σ)ppb in September,with an amplitude of 0.38 *** pattern is due to combined effects of variation in surface sources,vertical convection within the boundary layer and stratosphere to troposphere transportation(STE).The interannual variability in growth rate was partly driven by quasi-biennial oscillation(QBO)of tropical zonal wind through stratospheric transport into the *** to a cluster analysis of back trajectories and the corresponding average N_(2)O load,most air masses cover arid and semi-arid areas in inner Asia with low N_(2)O emissions,indicating that the atmospheric N_(2)O at the WLG represents the background N_(2)O level in central Eurasia.

关键词： Nitrous oxide(N_(2)O) Observation Long-term trend Seasonal variation Interannual variability

A study on the impact of heritage protection on urban carbon emissions

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Science China(Technological Sciences) 2024年第9期67卷 2675-2692页

作者： wang fang XUE PengCheng wang ShunYi liu YunHuai River Basin Habitats Research Center College of Architecture and Landscape ArchitecturePeking UniversityBeijing 100871China Shanxi Key Laboratory of Watershed Built Environment with Locality(Peking University) Peking UniversityBeijing 100871China College of Urban and Environmental Sciences Peking UniversityBeijing 100871China School of Computer Science Peking UniversityBeijing 100871China

A major goal of current international urban development and construction is to lower carbon *** exist between the preservation of historic cities and the development of low-carbon cities;however,the relationship between preservation of historic cities and carbon emissions remains *** study focuses on the cities in the“Yellow-Canal-Yangtze”watershed community,which includes the Yellow River Basin,Yangtze River Basin,and the Grand Canal region,and aims to verify the differences in CO_(2) emissions(CE)between historic and non-historic cities through multiple comparisons after ***,Ridge Regression was used to determine the impact and dimensions of the relevant variables associated with the preservation of historic cities on *** with national development goals,this study predicts changes in the CE of historic city preservation under different *** study found significant differences between historic and non-historic cities,as the total CE of historic cities is 2.42 times higher than that of non-historic *** dimension with the largest difference was the CE related to transportation,with aviation emissions of historic cities reaching 15.22 times higher than that of non-historic *** the preservation of historic cities has led to an overall increase in the CE,there is significant potential for CE reduction in land use,transportation infrastructure,and tourism development related to historic city *** the future,historic cities can contribute to China's carbon neutrality and the United Nation's development goals through the creation of larger historical preservation areas,more convenient public transportation,and more vibrant tourism.

关键词： CO_(2) emissions urban planning cultural heritage historic city “Yellow-Canal-Yangtze”watershed community

Bioeffects of different functionalized silica nano-particles on HaCaT cell line

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Chinese Science Bulletin 2006年第16期51卷 1939-1946页

作者： HE Xiaoxiao liu fang wang Kemin GE Jia QIN Dilan GONG Ping TAN Weihong State Key Laboratory of Chemo/Biosensing and Chemometrics Engineering Center for Biomedicine Institute of Life Science and Biological Technology Engineering Research Center for Bio-Nanotechnology of Hunan Province Changsha 410082 China

The bioeffects of silica nanoparticles (SiNP), phosphorylate-terminated nanoparticles (PO4- NP) and amino-terminated nanoparticles (NH2NP) on HaCaT cell line have been studied in this paper. The effects of the three kinds of functionalized silica nanoparticles on adherence, proliferation and cycle of HaCaT cells have been investigated. And the cel- lular uptake of the three kinds of functionalized silica nanoparticles by HaCaT cells has also been exam- ined. Results indicated that the bioeffects of the three kinds of functionalized nanoparticles on HaCaT cells were concentration-dependent. And the three kinds of functionalized nanoparticles all exhibited well bio- compatibility if the concentration was below 0.2 μg/μL. While the cytotoxicities of the three kinds of function- alized nanoparticles on HaCaT cells would increase with the increasing of nanoparticles concentration, and the following order was observed: NH2NP > SiNP > PO4NP. In addition, the quantity and rapidity of cellular uptake of nanoparticles by HaCaT cells were diverse due to the different functional groups. Under the same conditions, NH2NP was most and fast internalized by HaCaT cells, followed by SiNP, and PO4NP was the least and slowest. These results provided theoretical foundation for the safe applica- tion and further modification of silica nanoparticles, which could broaden the application of silica nano- particles in biomedicine.

关键词： SiNP PO4NP 纳米粒子 HaCaT细胞系生物效应