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Transcriptomic analysis of high-throughput sequencing about circular RNA under Meloidogyne incognita stress in tomato

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Transcriptomic analysis of high-throughput sequencing about ...

中国植物病理学会2021年学术年会

作者：杨帆 FAN Haiyan ZHAO Di WANG Yuanyuan ZHU Xiaofeng liu Xiaoyu duan Yuxi 陈立杰 College of Plant Protection Shenyang Agriculture University Analysis and Testing Center Shenyang Agriculture University College of Biotechnology Shenyang Agriculture University College of Science Shenyang Agriculture University

Circular RNA（circRNA） are newly discovered endogenous non-coding RNA（ncRNA） that play key roles in microRNA function and transcriptional *** some circRNAs have been identified in human and some animals,their function in the interaction between tomato plants and pathogen is unknown or *** root-knot nematode（RKN） severely affects plant growth and productivity,but the gene regulation of circRNA between tomato and RKN is *** this study,high-throughput sequencing was carried out to investigate the differential expression of circRNA and their biological functions in tomato inoculated with Meloidogyne incognita,then confirmed the expression of randomly selected RNAs with PCR and *** regulation network of circRNA was contructed constructed to predict the functions of these *** with the control group,18 circRNAs were upregulated and 11 circRNAs were downregulated in tomato inoculated with *** regulation network indicated that 9 circRNAs were found to act as sponges of the corresponding miRNA family,and the expression of SlMiIcircRNA8 and SIMiIcircRNA 10 were correlated with miR6022 and miR6024 family *** ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis were performed to ascertain the biological function of significantly dysregulated *** expression of those circRNAs was detected by quantitative real-time PCR to identify the differences in the expression of those *** analysis indicated that the dysregulated circRNAs may be participate in the genesis and progression of the resistance of plants against *** results may therefore be valuable in detecting novel transcripts,discovering new biomarkers for the resistance of plants against *** and expounding the pathogenic mechanisms of RKN disease.

关键词： circRNA M.incognita tomato high-throughput sequencing gene regulation

Synthesis of Magnetic Nickel Nanowires and Nanotubes

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近代物理研究所和兰州重离子加速器实验室年报：英文版 2009年第1期 108-109页

作者： Chen Yonghui duan Jinglai Yao Huijun Mo Dan Chen Yanfeng liu Jie Hou Mingdong Sun Youmei

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掌指关节假体个体化设计与应用:1例报告

中国组织工程研究 2019年第20期23卷 3164-3169页

作者：钟华刘军蔡国祥段少银厦门大学附属中山医院影像科福建省厦门市361004 吉林大学第二医院手外科吉林省长春市136000

背景:临床上掌指关节创伤严重毁损后大多直接截肢,此次研究主要尝试对毁损严重的掌指关节进行个体化假体置换,以期恢复患手部分功能。目的:通过3D打印技术与医用级聚醚醚酮材料机加工技术,探讨个体化掌指关节假体的设计、制作与临床应... 详细信息

背景:临床上掌指关节创伤严重毁损后大多直接截肢,此次研究主要尝试对毁损严重的掌指关节进行个体化假体置换,以期恢复患手部分功能。目的:通过3D打印技术与医用级聚醚醚酮材料机加工技术,探讨个体化掌指关节假体的设计、制作与临床应用。方法:(1)纳入右手机器绞伤男性患者1例,64岁。采用术前双手薄层螺旋CT扫描数据进行双手三维重建,计算机辅助设计右手掌指关节假体。该临床研究的实施符合《赫尔辛基宣言》和吉林大学第二医院对研究的相关伦理要求[批准号:(2018)年研审第(133),审批时间:2017-08-16];(2)将右手残存骨质、设计完善的右手掌指关节假体输入3D打印机设备和机加工设备,完成解剖形态个体化的右手掌指关节假体模型复现与制作;(3)利用打印模型进行术前模拟,术中即刻将掌指关节假体置入。结果与结论:(1)基于CT薄层扫描数据完成假体设计与制作,假体与母体吻合,手术复原手部解剖形态,皮瓣移植覆盖;(2)提示3D打印联合机加工技术实现个体化掌指关节假体的设计和应用为掌指关节毁损修复重建提供了一种方法,该技术的有益尝试可能成为骨修复、置换的新趋势。

关键词：掌指关节假体 CT数据三维重建 3D打印机加工

Upregulation of long noncoding RNA MEG3 Induced by Heat Stress Contributes to Apoptosis of Rat Cardiomyocytes via Promoting TR3 Transcription

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Upregulation of long noncoding RNA MEG3 Induced by Heat Stre...

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Xinxing Wang Zhaoli Chen Weili liu Tianhui Wang Ruifeng duan Yingying Li Lingling Pu Bingnan Deng Environmental Medicine Laboratory Tianjin Institute of Environmental and Operational Medicine

Mitochondria play a key role in cardiomyocytes apoptosis induced by heat *** previous study indicate that TR3 binding to mitochondria and induces apoptosis under ***,the alteration of TR3 responding environmental heat stress is far from fully *** present study,for the first time to the best of our knowledge,discovered that environmental thermal exposure led to MEG3 upregulation,consequently upregulate ELK1 transcription and increasing TR3 transcription,in turn resulting in decline of mitochondria membrane potential(ΔΨm) and apoptosis of ***,MEG3 upregulation was attributed to heat stress-induced c-Myc expression,while ELK1 upregulation was due to the increasing translation of c-Jun induced by ***,TR3 transcription was upregulated via increasing its transcription factor ELK1 the resultant from c-jun upregulation in heat stress ***,we uncover that heat stress exposure results in c-Myc induction and MEG3 expression elevation,further promotes c-Jun translation and in turn initiates ELK1 transcripton,leading to increase of TR3 protein trancription,and decline of mitochondria membrane potential(ΔΨm) as well as apoptosis of *** present study provided a significant insight into understanding the alteration and role of TR3 in heat stress cardiomyocytes.

关键词： TR3 ELK1 c-Myc MPTP Heat Stress

Ferroptosis and Its Role in Infectious Diseases

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Ferroptosis and Its Role in Infectious Diseases

中国微生物学会兽医微生物学专业委员会暨中国畜牧兽医学会生物制品学分会2021年学术论坛

作者： Mingcheng liu Bin He Mingyuan duan Shanshan Zhang Qiufen Wang Zhe Cheng Xiaojing Xia Jianhe Hu College of Animal Science and Veterinary Medicine Henan Institute of Science and Technology

Cell death is a vital process in *** the normal state,the organism clears damaged or harmful cells through cell death pathways to maintain the organism's tissue morphology and balance internal *** cells are exposed to irrecoverable extracellular or intracellular disturbances that disrupt their homeostasisand activate signal transduction cascades,

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Mo高压熔化的分子动力学模拟

高压物理学报 2008年第1期22卷 53-56页

作者：张弓木刘海风段素青北京应用物理与计算数学研究所北京100088

采用第一原理方法计算了钼在零温下的结构,表明钼在500 GPa以下一直保持bcc结构(常温),与实验一致。在零压附近计算了E-V关系,利用Murnaghan物态方程拟合得到了零压体积及其模量,与实验结果符合得很好。采用第一原理分子动力学模拟了钼... 详细信息

采用第一原理方法计算了钼在零温下的结构,表明钼在500 GPa以下一直保持bcc结构(常温),与实验一致。在零压附近计算了E-V关系,利用Murnaghan物态方程拟合得到了零压体积及其模量,与实验结果符合得很好。采用第一原理分子动力学模拟了钼的高压熔化性质。采用NVT系综计算了128个原子的系统,初始构形为bcc结构,体积分别为0.01548、0.01219、0.01098、0.00984、0.00910 nm3/atom,计算了几个温度点,拟合得到了熔化曲线,熔化温度明显高于金刚石压砧(DAC)实验结果;将初始构形改变为fcc结构,模拟其熔化特性,得到的熔化温度明显下降,与激光加载DAC实验结果一致,认为可能的原因是钼熔化后形成的液体结构类似于fcc结构,而不是常态时的bcc结构。

关键词：第一原理分子动力学高压熔化钼

Arabidopsis F-box gene At3g16740 involving in light-,GA-and ABA-mediated seed germination

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Arabidopsis F-box gene At3g16740 involving in light-,GA-and ...

2013全国植物生物学大会

作者： duan Guifang HE Reqing WANG Liqun ZHU Liaoliao ZHAO Fu TANG Dongying ZHAO Xiaoying liu Xuanming Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan University

F-box gene family is widely distributed in living organisms,from lower to higher organism. They play important roles in controlling many processes of plant growth and development, including phytohormone-signaling,floral development,and stress response,through the specific recognition of particular ***,we report that Arabidopsis F-box gene At3g16740 expressed in all organs in Arabidopsis,and higher in flower and *** mRNA expression was induced by blue,red,and far-red light,but suppressed in imbibed seed. Overexpression of At3g16740 under CaMV 35S promoter resulted in lower seed germination rate in far-red *** grown in the presence of different concentration of abscisic acid (ABA),transgenic lines showed increased sensitivity to ABA and had lower germination rate than wild *** germination phenotype of transgenic lines was also strongly suppressed by a Gibberellic acid(GA) biosynthesis inhibitor,*** of gene expression analysis indicated that At3g16740 might affect seed germination by controlling genes critical for GA and ABA *** two-hybrid assay results showed that At3g16740 protein interacted with Arabidopsis SKP1-homologs ASK1,ASK2 and ASK11,but their interaction were abolished when the F-box domain in the At3g16740 protein was deleted,suggesting that At3g16740 protein is most probably a component of an SCF complex.

关键词： light GA ABA At3g16740 Arabidopsis seed germination

Norovirus Infection and Histo-blood Group Antigens in Children Hospitalized with Diarrhea in Lulong and Chenzhou in China

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Biomedical and Environmental Sciences 2016年第4期29卷 286-289页

作者： SUN Xiao Man YAN Xiao Feng HE Zhi Gang Li Jing Xin GUO Ni Jun XU Zi Qian JIN Miao LI Dan Di ZHOU Yong Kang XIE Guang Cheng Pang Li Li ZHANG Qing liu Na duan Zhao Jun Key Laboratory of Medical Virology and Viral Diseases Ministry of Health of the People's Republic of China Beijing 102206 China National Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China Center for Disease Control and Prevention of Lulong County Lulong 066400 Hebei China Chenzhou No.1 People's Hospital Chenzhou 423000 Hunan China

Norovirus （NOV） is a pathogen that commonly causes viral diarrhea in children. Studies indicate that NoV recognizes human histo-blood group antigens （HBGAs） as cell attachment factors. In order to explore the correlation between of NoV infection and HBGAs, a cross-sectional study was conducted in children less than five years old who were hospitalized with diarrhea in two areas of China between November 2014 and February 2015. Of the paired stool and saliva samples taken from 424 children,

关键词： GII Norovirus Infection and Histo-blood Group Antigens in Children Hospitalized with Diarrhea in Lulong and Chenzhou in China

基于SSA-SVR的光纤压力传感器温度补偿研究

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电子器件 2023年第5期46卷 1268-1274页

作者：段松凯刘守兵河南机电职业学院电气工程学院河南郑州451191 河南工程学院电气信息学院河南郑州451191

针对光纤压力传感器存在的温度漂移问题,提出了基于麻雀搜索算法优化的支持向量回归(SSA-SVR)的温度补偿模型。对光纤压力传感器进行标定实验,并采用温度传感器LM35实时检测其工作温度,进而建立SVR温度补偿模型。利用SSA优化SVR的超参数... 详细信息

针对光纤压力传感器存在的温度漂移问题,提出了基于麻雀搜索算法优化的支持向量回归(SSA-SVR)的温度补偿模型。对光纤压力传感器进行标定实验,并采用温度传感器LM35实时检测其工作温度,进而建立SVR温度补偿模型。利用SSA优化SVR的超参数,以改善温度补偿模型的补偿精度。研究结果表明,利用基于SSA-SVR的温度补偿模型对光纤压力传感器进行温度补偿后,其灵敏度温度系数和相对误差系数均提高了两个数量级,而最大绝对误差和最大相对误差只有0.5004 kPa和0.2501%,达到了温度补偿的目的。

关键词：光纤压力传感器温度补偿麻雀搜索算法(SSA) 支持向量回归机(SVR) SSA-SVR模型