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Intestinal Transport and Biotransformation of Resibufogenin and Cinobufagin in Chan Su via HPLC/APCI-MS^n

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Chemical Research in Chinese Universities 2011年第3期27卷 380-384页

作者： HAN Tian-jiao WANG Qing SONG Feng-rui liu zhong-ying liu Zhi-qiang liu Shu-ying Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun Center of Mass SpectrometryChangchun 130022P.R.China Department of Endocrinology of China-Japan Union Hospital of Jilin University Changchun 130033P.R.China School of Pharmacy Jilin UniversityChangchun 130033P.R.China Graduate School of the Chinese Academy of Sciences Beijing 100049P.R.China

In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.

关键词： Chan Su Resibufogenin Cinobufagin Caco-2 cell monolayer Human intestinal bacteria

Systemic Nuclear Proteomics Researches on Change of Jurkat T Lymphocyte Cells Under Radiation

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Chemical Research in Chinese Universities 2010年第5期26卷 717-722页

作者： LIANG Feng liu zhong-ying liu Ning SONG Feng-rui ZHANG Xuan liu Zhi-qiang College of Pharmacy Jilin University Changehun 130021 P. R. China Changchun Center of Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Research Center for Genomic Medicine and MH Radiobiology Research Unit School of Public Health Jilin University Changchun 130021 P. R. China

Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.

关键词： Nuclear proteome Two-dimensional difference gel electrophoresis MALDI-TOF-MS Jurkat cell

不同造型白化绿茶滋味化学成分及感官品质分析

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南方农业学报 2024年第3期55卷 855-864页

作者：张小琴罗金龙李燕梁思慧刘忠英沈强贵州省茶叶研究所贵州贵阳550006

【目的】分析不同造型白化绿茶滋味化学成分及感官品质的差异,为开发白化茶树品种产品制定适宜的工艺流程提供参考依据。【方法】以白化茶树品种白叶1号鲜叶为原料,采用瓶式炒干机、双锅曲毫机和理条机3种设备分别制成卷曲形、颗粒形和... 详细信息

【目的】分析不同造型白化绿茶滋味化学成分及感官品质的差异,为开发白化茶树品种产品制定适宜的工艺流程提供参考依据。【方法】以白化茶树品种白叶1号鲜叶为原料,采用瓶式炒干机、双锅曲毫机和理条机3种设备分别制成卷曲形、颗粒形和松针形白化绿茶,运用高效液相色谱法(HPLC)等方法检测茶叶常规品质成分、氨基酸组分和儿茶素组分,采用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)和层次聚类分析(HCA)筛选关键特征品质成分,通过感官审评方法评价茶叶品质并分析其与特征成分的相关性。【结果】颗粒形白化绿茶茶多酚含量17.69%、酚氨比2.70,显著低于松针形和卷曲形(P<0.05,下同),鲜甜味氨基酸总量23.16 mg/g、茶氨酸含量18.14 mg/g、谷氨酸含量1.26 mg/g、天冬氨酸含量1.74 mg/g、丙氨酸含量0.13 mg/g、苏氨酸含量0.33 mg/g、缬氨酸含量0.26 mg/g,均显著高于松针形,同时鲜甜味氨基酸占比最大(84.74%)、苦味氨基酸占比最小(11.23%)。多元统计分析表明,不同造型白化绿茶差异品质成分主要有茶氨酸、谷氨酸、苏氨酸和甘氨酸4个鲜甜味氨基酸,异亮氨酸、酪氨酸、组氨酸、赖氨酸和精氨酸5个苦味氨基酸,甜略带苦味的脯氨酸和滋味未知的半胱氨酸,儿茶素没食子酸酯(CG)、表儿茶素没食子酸酯(ECG)和茶多酚3个酚类物质以及游离氨基酸和酚氨比;颗粒形白化绿茶的游离氨基酸总量及CG、茶氨酸、谷氨酸、苏氨酸、组氨酸和异亮氨酸含量明显高于松针形和卷曲形。感官审评总分以颗粒形(92.83分)和松针形(92.34分)显著高于卷曲形(84.43分),滋味因子得分以颗粒形(28.15分)最高、松针形(27.65分)次之,外形、汤色和香气因子得分均以颗粒形和松针形显著高于卷曲形,叶底因子得分以松针形(9.23分)最高。滋味因子与半胱氨酸、ECG、CG呈显著正相关,与精氨酸、酪氨酸、赖氨酸和脯氨酸呈极显著(P<0.01)或显著负相关。【结论】颗粒形白化绿茶鲜甜味氨基酸占比大,茶多酚含量和酚氨比较低,酯型儿茶素总量适中,在3种造型中滋味品质和综合品质最佳;松针形白化绿茶酯型儿茶素总量最高,苦味氨基酸总量最低,叶底得分最高,滋味得分次高,感官综合品质优于卷曲形。

关键词：白化绿茶造型滋味化学成分感官品质

Deformation Behavior of Mg-8 wt%Li Alloy under High-speed Impact

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Chinese Journal of Aeronautics 2006年第B12期19卷 235-238页

作者： SHA Gui-ying liu Chun-zhong YU Tao SHI Ji-hong Department of Materials Engineering Shenyang Institute of Aeronautical Engineering Shenyang 110034 China

Deformation behavior of the Mg-8 wt%Li alloy at high strain rate was studied by means of the Split Hopkinson Pressure Bar （with strain rate of 10^3 s^-1）. It is found that shear localization proves to be the main damage mode for the alloy during dynamic loading. Strain and strain rate arc the two necessary parameters affecting the occurrence of deformation and shear bands. Deformation bands begin to form when the strain and strain rate reach 0.20 and 1 900 s^-1 respectively and will develop gradually with the strain rate increasing. Besides, deformation bands will transform into shear bands when the strain and strain rate reach above 0.25 and 3 500 s^-1 separately.

关键词： strain rote strain shear localization Mg-Li alloy

Metabolomics analysis reveals the renal protective effect of Panax ginseng C. A. Mey in type 1 diabetic rats

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Chinese Journal of Natural Medicines 2022年第5期20卷 378-386页

作者： WANG Xin-Sen HU Ming-Xin GUAN Qing-Xiang MEN Li-Hui liu zhong-ying School of Pharmaceutical Sciences Jilin UniversityChangchun 130021China College of Basic Medical Sciences Jilin UniversityChangchun 130021China

The dry root and rhizome of Panax ginseng C. A. Mey has garnered much interest owing to its medicinal properties against diabetes and cardiovascular diseases. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS)-based metabolomics approach was used to illustrate the therapeutic mechanisms of ginseng extract on the serum and urinary metabolic profiles in streptozotocin-induced type 1 diabetes mellitus (T1DM) rats. Pharmacological and renal parameters in response to the administration of ginseng were also evaluated. In total, 16 serum endogenous metabolites and 14 urine endogenous metabolites, including pyruvic acid, indoleacetic acid, and phenylacetylglycine, were identified as potential biomarkers for diabetes. Pathway enrichment and network analysis revealed that the biomarkers modulated by ginseng were primarily involved in phenylalanine and pyruvate metabolism, as well as in arginine biosynthesis. Moreover, the levels of several renal injury-related biomarkers in T1DM rats were significantly restored following treatment with ginseng. The administration of the extract helped maintain tissue structure integrity and ameliorated renal injury. The findings suggest that the regulatory effect of ginseng extract on T1DM involves metabolic management of diabetic rats, which subsequently attenuates T1DM-induced early renal dysfunction.

关键词： Panax ginseng C.A.Mey Type 1 diabetes Metabolomics

Non-contact and full-field online monitoring of curing temperature during the in-situ heating process based on deep learning

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Advances in Manufacturing 2024年第1期12卷 167-176页

作者： Qiang-Qiang liu Shu-Ting liu ying-Guang Li Xu liu Xiao-zhong Hao College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing210016People’s Republic of China School of Mechanical and Power Engineering Nanjing Tech UniversityNanjing211816People’s Republic of China

Online monitoring of the curing temperature field is essential to improving the quality and efficiency of the manufacturing process of composite *** embedded sensor-based technologies have difficulty monitoring the full temperature field or have to introduce heterogeneous items that could have an undesired impact on the *** this paper,a non-contact,full-field monitoring method based on deep learning that predicts the internal temperature field of composite parts in real time using surface temperature measurements of auxiliary materials is *** the proposed method,an average temperature monitoring accuracy of 97%is achieved in various heating *** addition,this method also demonstrates satisfying feasibility when a stronger thermal barrier covers the *** method was experimentally validated during the self-resistance electric heating process,in which the monitoring accuracy reached 93.1%.This method can potentially be applied to automated manufacturing and process control in the composites industry.

关键词： Online monitoring Curing temperature field Deep learning(DL) In-situ heating

Protective Effects of Ginsenoside Rb1 on Septic Rats and Its Mechanism

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Biomedical and Environmental Sciences 2014年第4期27卷 300-303页

作者： WU Li Li JIA Bao Hui SUN Jian CHEN Jun Xi liu zhong ying liu Yuan Department of Emergency and ICU Fourth Affiliated Hospital of Nanchang University School of Life Sciences and Food Engineering Nanchang University

This study aims to observe the protective effects of ginsenoside Rbl on liver and lung in rats with septic shock and reveal its mechanism. Rats were randomly divided into three groups： sham, cecal ligation and puncture （CLP）, and CLP with ginsenoside Rb1. Then, the survival rate, arterial blood pressure, TLR4 mRNA, and TNF-α levels were determined. The liver and lung tissues were stained with hematoxylin-eosin （HE）. The overall survival rate of the Rb1 group was significantly higher than that of the CLP group. Mean arterial blood pressure went down in both the CLP and Rb1 groups after CLP, and there was a significant difference both in the sham and Rb1 groups when compared with the CLP group. The Rb1 treatment group had markedly lower TLR4 mRNA expression and TNF-a levels than the CLP group. In the CLP group, pathology showed swelling, degeneration, necrosis, and neutrophii infiltration in the liver and alveolar epithelial cells. However, in the Rb1 group, there was mild degeneration and slight neutrophil infiltration, but no obvious necrosis. Rb1 may improve the survival rate, ameliorate arterial blood pressure, and protect the liver and lung in septic shock rats by downregulating the expression of TLR4 mRNA and inhibiting the production of TNF-α.

关键词： Rb CLP Protective Effects of Ginsenoside Rb1 on Septic Rats and Its Mechanism

Improvement of mechanical properties and investigation of strengthening mechanisms on the Ti_(3)AlC_(2) ceramic with nanosized WC addition

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Journal of Advanced Ceramics 2024年第6期13卷 861-876页

作者： Yi zhong ying liu Qinkai Jiang Na Jin Zifeng Lin Jinwen Ye School of Materials Science&Engineering Sichuan UniversityChengdu 610065China Yibin R&D Park Yibin Industrial Technology Research InstituteSichuan UniversityYibin 644005China

Ti_(3)AlC_(2) (TAC) has great potential for use as an ablation material in aerospace applications due to its great oxidation/ablation resistance, but its high-temperature strength and thermal shock resistance still have much room for simultaneous improvement under fast temperature variation conditions. Herein, we used Ti_(3)AlC_(2) and WC powders as raw materials and successfully fabricated textured (Ti,W)_(3)AlC_(2) ceramic with small amounts of TiC and Al_(2)O_(3), and room temperature mechanical properties such as flexural strength (1146±46.9 MPa), fracture toughness (11.78±0.44 MPa·m^(1/2)), and hardness (5.81±0.11 GPa) at 5 wt% WC addition were achieved. The high-temperature strength of the ceramic was significantly improved, and better thermal shock resistance from 298 to 1173 K was simultaneously acquired together with the regulation of the elastic modulus, thermal conductivity, and thermal expansion coefficient, providing (Ti,W)_(3)AlC_(2) with more possibilities for fast-temperature variation applications. Strengthening and toughening mechanisms were proposed. Scanning transmission electron microscopy high-angle annular dark-field imaging (STEM-HADDF) showed that W randomly replaced the Ti1 and Ti2 sites of Ti_(3)AlC_(2), providing a good reference for establishing crystal models, and further density functional theory (DFT) calculations based on these models indicated a higher fracture energy of (Ti,W)_(3)AlC_(2) along different crystal planes, providing superior resistance to transgranular fracture;a lower mismatch degree of (Ti,W)_(3)AlC_(2)/Al_(2)O_(3) resulted in stronger interface bonding, resulting in greater resistance to intergranular fracture as well as more balanced stress distributions at different interfaces.

关键词： mechanical properties solid solution MAX phase transmission electron microscopy(TEM) ceramic atomic structure

ON THE 3-D PULSATING SOURCE OF MICHELL TYPE WITH FORWARD SPEED

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Journal of Hydrodynamics 1995年第2期7卷 84-95页

作者： Miao Guo-ping liu ying-zhong Yang Qin-zheng liu Zi-yuan(Shanghai Jiao Tong Univ., shanghai 200030 P. R. China) Shanghai Jiaotong Univ Shanghai China

An expression of the 3-D pulsating source of Michell type with forward speed for infinite water depth is derived in the present paper based on the Fourier transformation and contour integration technique. Emphases are laid on the discussion of the mechanical feature of the source and the radiation behavior far from the source. The relative importance of each components of the pulsating source is discussed in detail. Compared with the commonly used pulsating source of Haskind type, the present expression has the merits of simplicity for numerical treatment and being more meaningful in its physical explanation. The Michell type expression of the 3-D pulsating source with forward speed can be widely used for the estimation of motions and wave forces for ships and offshore structures in waves with forward speed. The radiative behavior of the source in the far field may also give a reasonabel description of the radiation condition or the open boundary condition for other numerical methods, such as the Rankine source method etc.

关键词： D pulsating source with forward speed radiative behavior of the source,Fourier transform