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Expression and regulation of plasminogen activator and plasminogen activator inhibitor type-1 in rat epididymis

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Chinese Science Bulletin 1997年第9期42卷 779-783页

作者： Zhou, HM Zhang, T liu, yx CHINESE ACAD SCI INST ZOOLSTATE KEY LAB REPROD BIOLBEIJING 100080PEOPLES R CHINA

EPIDIDYMIS is the site where spermatozoa released from testis undergo maturation and become capable of fertilizing *** epididymal secretions including enzymes,nutrients and hor-mones are believed to be essential for sperm *** proteolysis contributes toloss or modification of surface molecules of spermatozoa,which is an important aspect of

关键词： plasminogen activator plasminogen activator inhibitor human chorionic gonadotropin/luteinizing hormone epi-didymis.

Role of plasminogen activator and plasminogen activator inhibitor type-1 in luteolysis——a minireview

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Chinese Science Bulletin 1997年第23期42卷 1994-2000页

作者： liu, yx Chen, YJ Feng, QA Hu, ZY Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

This minireview summarized our recent studies on the role of plasminogen activator (PA) and inhibitor type-1 (PAI-1) in luteolysis. We have demonstrated that (1) both tissue type and urokinase type plasminogen activators (tPA and uPA) and a plasminogen activator inhibitor type-1 (PAI-1) were present in the corpus luteum of rat and rhesus monkey; (2) decrease in progesterone production in corpus luteum was well correlated with a sharp increase in tPA (but not uPA) and PAI-1 secretion; (3) exogenous tPA decreased luteal progesterone synthesis while monoclonal antibodies increased progesterone production; (4) interferon y inhibited luteal progesterone synthesis and stimulated tPA production while LH plus pro-lactin increased progesterone production and decreased tPA (but not uPA) activity in cultured luteal cells; (5) increase in proteolysis in the corpus luteum was also correlated with decrease in progesterone production in mouse. These data suggest that local degradation of extracellular matrix

关键词： tissue type plasminogen activator (tPA) plasminogen activator inhibitor type-1 (PAI-1) Iuteolysis.

Identification of secretion sites of tissue plasminogen activator and plasminogen activator inhibitor type-1 in basal plates of human and rhesus monkey placentae

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Chinese Science Bulletin 1997年第12期42卷 1030-1033页

作者： liu, yx Hu, ZY Zou, RJ Ockleford, CD State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China Primate Research Center Kunming Institute of Zdogy Chinese Academy of Sciences Kunming China Department of Pre-clinical Sciences University of Leicester Leicester UK

INCREASING evidence has demonstrated that the locally controlled proteolytic activity generatedby coordinated expression of tPA and PAI-1 in different tissues may play an important role inmany reproductive events. These are largely related to fibrinolytic activity. They include folli-cle rupture, luteolysis, spermatogenesis and trophoblast implantation. Parturition, whichis a complex process, may also be associated with tissue destruction. Detachment of placentaldecidua and partial breakdown of fetal membranes are possible examples. It has been

关键词： tissue plasminogen activator (tPA) plasminogen activator inhibitor type-1 (PAI-1) placental basal plate im-munofluorescence parturition.

8-chloroadenosine induces apoptosis in human MOLT-4 cell line

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Chinese Science Bulletin 1997年第7期42卷 592-597页

作者： Wang, XT Zheng, DX liu, R liu, yx Xiao, S Min, JM Zheng, P Zhang, LH liu, SL CHINESE ACAD MED SCI PEKING UNION MED COLLSTATE KEY LAB MED MOL BIOLBEIJING 100005PEOPLES R CHINA

THE 8-chloroadenosine (8CA) is a novel analog of adenosine synthesized in the National Research Laboratories of Natural and Biomimetic Drugs. Fang et al. reported that 8CA is capable of inhibiting the cell growth in v... 详细信息

关键词： 8-chloroadenosine apoptosis MOLT-4 cells.

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动态测验的发展及应用

中国组织工程研究与临床康复 2007年第52期11卷 10687-10689页

作者：齐力刘跃雄方平首都师范大学心理系北京市100037

目的:动态测验是评估人类能力的一种新的测量方法。综合分析动态测验的理论和发展,并对动态测验的实证研究进行重点介绍,以期对国内动态测验的实证研究有所帮助。方法:通过Proquest数据库检索2000-01/2006-05、关键词为"动态测验"及"动... 详细信息

目的:动态测验是评估人类能力的一种新的测量方法。综合分析动态测验的理论和发展,并对动态测验的实证研究进行重点介绍,以期对国内动态测验的实证研究有所帮助。方法:通过Proquest数据库检索2000-01/2006-05、关键词为"动态测验"及"动态评估"的外文文献。同时,通过中国期刊网全文数据库检索1996-01/2006-05同样关键词的相关中文文献。根据检索的文献论述动态测验的相关知识,讨论动态测验的发展,并按领域对动态测验的实证研究进行综述。结果:动态测验逐渐被应用于神经心理学、精神病理学、教育等方面,并被证实为较好的测量方法。但在国内,动态测验的实证研究少之又少。结论:动态测验是对传统测验的一个必要的有益的补充,随着动态测验理论和实证研究的不断深入,动态测验必将会发展成为很有潜力的测量工具。

关键词：动态测验认知能力应用

Rapid nongenomic inhibitory effects of glucocorticoids on phagocytosis and superoxide anion production by macrophages

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第二军医大学学报 2005年第10期26卷 1106-1106页

作者： Long F Wang yx liu L Zhou J Cui RY Jiang CL Department of Nautical Medicine Second Military Medical UniversityShanghai 200433 China

微重力条件下液相烧结态W—Ni合金的显微结构变化

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钨钼材料 1996年第4期 47-48页

作者： liu.,yx 钟培全

Preparation and analysis of cSNP chip on hepatocellular carcinoma-related genes

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Hepatobiliary & Pancreatic Diseases International 2005年第3期4卷 398-402页

作者： College of Life Sciences, Shenzhen University, Shenzhen 518060, China( Wang J) College of Life Sciences, Nankai University, Tianjin 300071 ,China( Ni H, Chen L, Chen CB and Song WQ) and Tianjin Cancer Hospital, Tianjin 300060 , China(liu yx) College of Life Sciences Shenzhen University Shenzhen 518060 China. wangjuannk@***

The understanding of cSNPs of cancer-related genes harboring in high frequency loss regions of tumor chromosomes can advance the disclosure of genetic and variant mechanisms of tumorigenesis,and the investigation of cancer susceptibility. In preparing a gene chip for detecting polymorphisms on coding region of genes in hepatocellular carcinoma tissues, some cSNPs are of interest for their potential links with phenotype. METHODS: The genes harboring in loss regions with high frequency of hepatocellular carcinoma (HCC) were selected, the related information of cSNP sequences was obtained from the SNP database (dbSNP) of the National Center for Biotechnology Information (NCBI). Then appropriate primers and oligonucleotide probes were designed according to the SNP sites, and a gene chip for the detection of SNPs was constructed. The chip included 48 cSNPs of 25 hepatocellular carcinoma-related genes. The PCR products labeled by Dig-dUTP were hybridized with the cSNP chip. RESULTS:The sensitivity, influence by probe concentration, and reiteration of the chip were detected, with a high sensitivity of 6 × 10-3 ng/μl. The signal of hybridization was reduced with a lower concentration of probe. Seven polymorphisms of caspase 9 (rs2308941) C →T and DOK2 (rs2242241) T→G, 6 of polymorphisms of EGFL3 (rs947345) A→G, caspase 9 (rs2308938) C→G and PHGDH (rs1801955)T→A, 5 of polymorphisms of E2F2(rs3218170) G→A,4 of polymorphisms of MUTYH( rs1140507) T→C and BNIP3L(rs1055806)G→T, and 1 of polymorphism of TNFRSF1B (rs1061622)T→G were detected by the chip in the tissues of 10 HCC. Samples of caspase 9 (rs2308941G) and (rs2308941A) were verified by PCR-SSCP and sequencing. CONCLUSION:The cSNP chip of hepatocellular carcinoma-related genes can accelerate the discovery of polymorphic markers on hepatocellular carcinoma.

关键词： hepatocellular carcinoma gene chip coding region single nucleotide polymorphism

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钙调神经磷酸酶在心血管系统中的作用

中国组织工程研究与临床康复 2007年第30期11卷 6028-6031页

作者：刘英新徐志香张莹刘英华解放军济南军区总医院保健四科二区山东省济南市250031 山东大学齐鲁医院骨外科山东省济南市250012

目的:钙调节神经磷酸酶是目前惟一已知的细胞内钙/钙调素依赖性丝氨酸/苏氨酸蛋白磷酸酶,在细胞信号传递过程中直接受钙的调节,起去磷酸化作用。就钙调节神经磷酸酶的酶学特性、分布、生物学功能及其与心血管系统的关系作一综述。资料来... 详细信息

目的:钙调节神经磷酸酶是目前惟一已知的细胞内钙/钙调素依赖性丝氨酸/苏氨酸蛋白磷酸酶,在细胞信号传递过程中直接受钙的调节,起去磷酸化作用。就钙调节神经磷酸酶的酶学特性、分布、生物学功能及其与心血管系统的关系作一综述。资料来源:检索Medline1995-01/2005-12及清华同方数据库1994-01/2005-12的相关文章,检索词“calcineurin”,并限定文章语言种类为English。检索词“钙调节神经磷酸酶”,限定文章语言种类为中文。资料选择:对资料进行初审,纳入标准:钙调节神经磷酸酶的酶学特性、分布、生物学功能及其与心血管系统关系的实验研究文章。排除标准:重复研究、综述、Meta分析。资料提炼:共收集到95篇关于钙调节神经磷酸酶的酶学特性、分布、生物学功能及其与心血管系统关系的实验研究的文献,30篇符合纳入标准。排除的65篇中包括重复研究、综述、Meta分析。资料综合:①近年发现,钙调节神经磷酸酶-T细胞活化核因子途径广泛存在于心肌骨骼肌、血管平滑肌细胞等,参与心肌和骨骼肌肥大、成纤维细胞增殖的发生。②钙调节神经磷酸酶为一个催化亚单位(CnA)和一个调节亚单位(CnB)组成的异源二聚体,有3种主要同工酶:CaNα,CaNβ,CaNγ。钙/钙调素与钙调节神经磷酸酶结合后诱发CnA构象的改变,致使自身抑制域移位,暴露出活性位点,产生钙调节神经磷酸酶的活化。③钙调节神经磷酸酶使胞浆转录因子NF-ATS脱磷酸化,暴露其核定位信号,NF-ATS得以转位入核,与核内其他转录因子协同调节多种基因的活化。在免疫应答中的重要作用,参与细胞生长,凋亡。④钙调节神经磷酸酶信号通路启动血管发育及后期血管与周围组织之间的有联,钙/钙调节神经磷酸酶/NF-ATC在正常心瓣和心膈形成的信号转导过程中发挥重要作用,对心脏的正常形态学发生至关重要。结论:钙调节神经磷酸酶在心血管系统的正常生理结构与功能的维持以及病理生理过程中均发挥着重要作用。调节神经磷酸酶信号通路在心血管系统生理及病理生理中的反作用的证实,以及对其作用机制、抑制剂的深入研究,对探讨心血管疾病的发病机制、正常心血管功能的调节机制有重要意义。

关键词：钙调蛋白结合蛋白质类心血管系统心肌/酶学信号传导