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Serotonin enrichment of rice endosperm by metabolic engineering

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The Crop Journal 2023年第6期11卷 1943-1948页

作者： Qingqing Yang Yan Tan Ying Ye Dongsheng Zhao qiaoquan liu Key Laboratory of Crop Genomics and Molecular Breeding of Jiangsu Province/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education College of AgricultureYangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009JiangsuChina

In animals,serotonin is a neurotransmitter and mood *** plants,serotonin functions in energy acquisition,tissue maintenance,delay of senescence,and response to biotic and abiotic *** this study,we examined the effect of serotonin enrichment of rice endosperm on plant growth,endosperm development,and grain *** do so,TDCs and T5H were selected as targets for serotonin *** of TDC1 or TDC3 increased serotonin accumulation relative to overexpression of T5H in rice *** lines of target genes driven by the Gt1 promoter showed better field performance than those driven by the Ubi *** of T5H showed little effect on plant growth or grain physicochemical *** neuronal cell culture assays,serotonin induced neuroprotective action against *** of rice cultivars with high serotonin content may be beneficial for health and nutrition.

关键词： Rice endosperm Serotonin Metabolic engineering TDCs T5H

Post-genomics revolution in the design of premiumquality rice in a high-yieldingbackground to meet consumer demands in the 21st century

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Plant Communications 2022年第3期3卷 1-16页

作者： Nese Sreenivasulu Changquan Zhang Rhowell NTiozon qiaoquan liu Consumer Driven Grain Quality and Nutrition Unit Rice Breeding and Innovation PlatformInternational Rice Research InstituteLos Ban˜os 4030Philippines Key Laboratory of Plant Functional Genomics of the Ministry of Education Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingCollege of AgricultureYangzhou UniversityYangzhou 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhou 225009China Max Planck Institute of Molecular Plant Physiology Am M€uhlenberg 114476 Potsdam-GolmGermany

The eating and cooking quality(ECQ)of rice is critical for determining its economic value in the marketplace and promoting consumer *** has therefore been of paramount importance in rice breeding ***,we highlight advances in genetic studies of ECQ and discuss prospects for further enhancement of ECQ in *** in gene-and genome-editing techniques have enabled improvements in rice *** genes and quantitative trait loci(QTLs)have been shown to regulate starch composition,thereby affecting amylose content and thermal and pasting properties.A limited number of genes/QTLs have been identified for other ECQ properties such as protein content and ***-assisted breeding has identified rare alleles in diverse genetic resources that are associated with superior ECQ *** post-genomics-driven information summarized in this review is relevant for augmenting current breeding strategies to meet consumer preferences and growing population demands.

关键词： amylose amylopectin eating and cooking quality genetics genome editing texture

Starch biosynthesis in cereal endosperms: An updated review over the last decade

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Plant Communications 2021年第5期2卷 1-22页

作者： Lichun Huang Hongyan Tan Changquan Zhang Qianfeng Li qiaoquan liu Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding State Key Laboratory of Hybrid RiceKey Laboratory of Plant Functional Genomics of the Ministry of EducationCollege of AgricultureYangzhou UniversityYangzhou 225009China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province Key Laboratory of Crop Genetics and Physiology of Jiangsu ProvinceYangzhou UniversityYangzhou 225009China

Starch is a vital energy source for living organisms and is a key raw material and additive in the food and non-food *** has received continuous attention in multiple research *** endosperm of cereals(e.g.,rice,corn,wheat,and barley)is the most important site for the synthesis of storage *** 2010,several excellent reviews summarized key progress in various fields of starch research,serving as important references for subsequent *** the past 10 years,many achievements have been made in the study of starch synthesis and regulation in *** present review provides an update on research progress in starch synthesis of cereal endosperms over the past decade,focusing on new enzymes and non-enzymatic proteins involved in starch synthesis,regulatory networks of starch synthesis,and the use of elite alleles of starch synthesis-related genes in cereal breeding *** also provide perspectives on future research directions that will further our understanding of cereal starch biosynthesis and regulation to support the rational design of ideal quality grain.

关键词： cereal starch biosynthesis regulation network endosperm development quality improvement

DNA methylation modification in heterosis initiation through analyzing rice hybrid contemporary seeds

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The Crop Journal 2021年第5期9卷 1179-1190页

作者： Shirong Zhou Meiqing Xing Zhilong Zhao Yincong Gu Yunping Xiao qiaoquan liu Hongwei Xue State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095JiangsuChina Joint Center for Single Cell Biology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Shanghai OEbiotech Shanghai 201210China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhou 225009JiangsuChina

Heterosis is an important biological phenomenon and widely applied in *** many studies have been performed by using vegetative organs of F_(1) hybrid plants,how heterosis (or hybrid vigor) is initiated and formed,particularly the underlying molecular mechanism,remain *** contemporary seeds of rice indica varieties 9311 and PA64 were innovatively used and analysis of DNA methylome of embryo and endosperm at early developing stages revealed the globally decreased DNA ***,especially those relate to hormones function and transcriptional regulation present non-additive *** identified heterosis-related superior genes are non-additively methylated in early developing hybrid contemporary seeds,suggesting that key genes/loci responsible for heterosis are epigenetically modified even in early developing hybrid seeds and hypomethylation of hybrid seeds after cross-pollination finally result in the long-term transcriptional change of F_(1) hybrid vegetative tissues after germination,demonstrating that altered DNA methylation in hybrid seeds is essential for initiation regulation and maintenance of heterosis exhibiting in F_(1) hybrid ***,a large number of genes show non-additive methylation in the endosperm of reciprocal hybrids,suggesting that endosperm might also contribute to heterosis.

关键词： DNA methylome Heterosis Rice Reciprocally hybrid seeds Embryo Endosperm

Introduction of antisense waxy gene into main parent lines of indica hybrid rice

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Chinese Science Bulletin 2002年第14期47卷 1192-1197页

作者： CHEN Xiuhua liu qiaoquan WANG Zongyang WANG Xingwen CAI Xiuling ZHANG Jingliu GU Minghong Key Laboratory of Biotechnology of Jiangsu Province Yangzhou UniversityYangzhou 225009China Shanghai Institute of Plant Physiology and Ecology Chinese Academy of ScienceShanghai 200032China

Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains

关键词： transgenic rice indica rice maintain line antisense waxy gene amylose content.

Two ABCI family transporters,OsABCI15 and OsABCI16,are involved in grain-filling in rice

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Journal of Genetics and Genomics 2024年第5期51卷 492-506页

作者： Bin Ma Xiubiao Cao Xiaoyuan Li Zhong Bian Qi-Qi Zhang Zijun Fang Jiyun liu Qun Li qiaoquan liu Lin Zhang Zuhua He Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology Agricultural College of Yangzhou UniversityYangzhouJiangsu 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhouJiangsu 225009China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology&EcologyChinese Academy of SciencesShanghai 200032China Institute of Biotechnology Hangzhou Academy of Agricultural SciencesHangzhouZhejiang 310024China Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education Yangzhou UniversityYangzhouJiangsu 225009China

Seed development is critical for plant reproduction and crop yield,with panicle seed-setting rate,grain-filling,and grain weight being key seed characteristics for yield ***,few genes are known to regulate grain ***,we identify two adenosine triphosphate(ATP)-binding cassette(ABC)I-type transporter genes,OsABCI15 and OsABCI16,involved in rice *** genes are highly expressed in developing seeds,and their proteins are localized to the plasma membrane and ***,knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate,caused predominantly by the severe empty pericarp phenotype,which differs from the previously reported low seed-setting phenotype resulting from failed *** analysis indicates that OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain ***,overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown ***,the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development,as demonstrated by their disruption in transgenic ***,ourﬁndings reveal the important roles of two ABC transporters in cereal grain filling,highlighting their value in crop yield improvement.

关键词： ABC transporters Rice Grain filling Ion homeostasis Grain yield

Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311

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The Crop Journal 2023年第3期11卷 887-894页

作者： Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao qiaoquan liu Shuzhu Tang Honggen Zhang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina School of Life Sciences Nantong UniversityNantong 226019JiangsuChina

The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon *** used widely in three-line indica *** identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type *** we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type *** developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting *** analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were ***,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene *** results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.

关键词： Rice WA-type CMS Restorer of fertility(Rf)gene Gene mapping

A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency

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Journal of Integrative Plant Biology 2021年第5期63卷 889-901页

作者： Changquan Zhang Yong Yang Shengjie Chen Xueju liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan Shuzhu Tang Minghong Gu qiaoquan liu Key Laboratory of Crop Genomics and Molecular Breeding of Jiangsu Province State Key Laboratory of Hybrid RiceYangzhou UniversityYangzhou 225009China Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Co‐Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province Yangzhou UniversityYangzhou 225009China State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina

In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs.

关键词： allelic variation amylose content(AC) eating and cooking quality(ECQ) grain appearance Oryza sativa L. Waxy

Introgression of lac6/tl1/du13 improves the palatability of japonica rice

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The Crop Journal 2024年第4期12卷 1259-1265页

作者： Yong Yang Tao Zhang Yuliang Shi Yan Lu Qianfeng Li Xiaolei Fan Lichun Huang Lu Chen Xuetang Song Qing liu Changquan Zhang qiaoquan liu Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education Agricultural College of Yangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009JiangsuChina Jiang Su(Wu Jin)Rice Research Institute Changzhou 213175JiangsuChina

Amylose content(AC)is a crucial determinant of the eating and cooking quality(ECQ)of rice,with low AC varieties exhibiting a softer texture and greater stickiness-attributes that enhance palatability and are desirable in specific culinary *** harness these traits,significant efforts have been made to manipulate AC to improve rice *** research utilized the MutMap+approach to identify LAC6/TL1,a gene that is an allele of Du13,responsible for low ***6 encodes a C2H2 zinc finger protein,which specifically increases the splicing efficiency of the Wxb allele without affecting the Wxa *** studies of LAC6 revealed that its proper integration could rectify the undesirable AC phenotype,whereas mutations within this gene led to reduced AC and were associated with shorter grain length and decreased thousand-grain *** these drawbacks,such mutations positively impact rice palatability,presenting a trade-off between grain size and eating *** address the challenges posed by the reduced grain weight associated with LAC6 mutations,we developed a specific molecular marker for LAC6,which has been effectively used in breeding programs to select lac6/tl1/du13 homozygous individuals with larger grain *** findings demonstrate that the“small grain”trait associated with lac6/tl1/du13 can be effectively mitigated through combined phenotype-based and marker-assisted *** study highlights the potential of lac6/tl1/du13 as a valuable gene for breeding novel,high-quality soft rice varieties through targeted breeding strategies.

关键词： Rice Dull endosperm Amylose content Wx Breeding