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Study on enhancement of fibronectin-mediated bacillus Calmette-Guerin attachment to urinary bladder wall in rabbits

上海交通大学学报（医学版） 2007年第11期27卷 1375-1375页

作者： Shen ZJ Wang Y Ding gq Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai 200025 China

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以消化性溃疡为首发表现的恶性淋巴瘤1例

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世界华人消化杂志 2006年第9期14卷 930-931页

作者：瞿国强安徽省铜陵市第四人民医院内科安徽省铜陵市244000

1例以消化性溃疡为首发表现,同时发现浅表淋巴结肿大,考虑胃癌伴淋巴结转移,但反复胃镜下活检病理不支持,最终取浅表淋巴结活检确诊为恶性淋巴瘤．胃恶性淋巴瘤常误诊为胃癌、胃溃疡等疾病,临床医生应提高警惕．

关键词：消化性溃疡恶性淋巴瘤

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Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) supports adhesion and migration of mesenchymal stem cells and tenocytes

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World Journal of Stem Cells 2012年第9期4卷 94-100页

作者： Alex J Lomas George gq Chen Alicia J El Haj Nicholas R Forsyth Guy Hilton Research Centre Keele University Stoke on Trent ST4 7QB United Kingdom Department Biological Sciences and Biotechnology School of Life Science Tsinghua University

AIM: To establish the potential of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) as a material for tendon repair. METHODS: The biocompatibility of PHBHHx with both rat tenocytes (rT) and human mesenchymal stem cells (hMSC) was explored by monitoring adhesive characteristics on films of varying weight/volume ratios coupled to a culture atmosphere of either 21% O2 (air) or 2% O2 (physiological normoxia). The diameter and stiffness of PHBHHx films was established using optical coherence tomography and mechanical testing, respectively. RESULTS: Film thickness correlated directly with weight/volume PHBHHx (r2 = 0.9473) ranging from 0.1 mm (0.8% weight/volume) to 0.19 mm (2.4% weight/volume). Film stiffness on the other hand displayed a biphasic response which increased rapidly at values > 1.6% weight/volume. Optimal cell attachment of rT required films of ≥ 1.6% and ≥ 2.0% weight/volume PHBHHx in 2% O2 and 21% O2 respectively. A qualitative adhesion increase was noted for hMSC in films ≥ 1.2% weight/volume, becoming significant at 2% weight/volume in 2% O2. An increase in cell adhesion was also noted with ≥ 2% weight/volume PHBHHx in 21% O2. Cell migration into films was not observed. CONCLUSION: This evaluation demonstrates that PHBHHx is a suitable polymer for future cell/polymer replacement strategies in tendon repair.

关键词： Mesenchymal stem cell Tenocytes Polyhydroxyalkanoates Hypoxia Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)

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Decreased Pollen Viability and Thicken Pollen Intine in Antisense Silenced Brassica campestris Mutant of BcMF19

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Journal of Integrative Agriculture 2014年第5期13卷 954-962页

作者： liu Jin-long GAO Ming-hui liu Ying CAO Jia-shu Laboratory of Cell and Molecular Biology Institute of Vegetable Science Zhejiang University

Brassica campestris male fertility 19 （BcMF19; GenBank accession number gq902048.1）, a gene that is specially expressed in tapetum cells and microspores during anther development in B. campestris ssp. chinensis, which is learned from the previous in situ hybridization study. In the present study, we constructed antisense-silenced plants of BcMF19 using B. campestris ssp. chinensis to validate this prediction. The morphology of the pistils, long anthers, and short anthers was significantly affected in 35sbcmf19 compared with the control samples. 4＇-6-Diamidino-2-phenylindole staining revealed that two generative nuclei and one large vegetative nucleus were not affected in the mutant compared with control. Statistical analysis of Alexander＇s staining results showed that 96% of the control pollen grains had vitality, whereas only 86% of the mutant pollen grains did. Under scanning electron microscopy, the mutant demonstrated numerous abnormal pollen grains and resembled dried persimmon. The frequency of normal pollen grains was approximately 18%. Under transmission electron microscopy, the pollen intine during the binucleate and mature pollen stages in 35sbcmf19 exhibited abnormal thickening, especially at the germinal furrows, compared with control. In vitro pollen germination test showed that the tips of the mutant pollen tubes transformed into globular alveoli and stopped growing compared with control. On the other hand, in vivo pollen germination test suggested that BcMF19 affected the pollen tube extension in the pistil. These findings indicate that BcMF19 is essential to the pollen development and pollen tube extension orB. campestris ssp. chinensis.

关键词： Brassica rapa ssp. chinensis PGIP pollen polygalacturonase-inhibiting protein tapetum pollen germination

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FINITE ELEMENT MODELLING OF COMPLEX 3D STATIC AND DYNAMIC CRACK PROPAGATION BY EMBEDDING COHESIVE ELEMENTS IN ABAQUS

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Acta Mechanica Solida Sinica 2010年第3期23卷 271-282页

作者： Xiangting Su Zhenjun Yang Guohua liu College of Civil Engineering and Architecture Zhejiang University Hangzhou 310027 China Department of Engineering the University of Liverpool L69 3GQ UK

This study proposes an algorithm of embedding cohesive elements in Abaqus and develops the computer code to model 3D complex cragk propagation in quasi-brittle materials in a relatively easy and efficient manner. The cohesive elements with softening traction-separation relations and damage initiation and evolution laws are embedded between solid elements in regions of interest in the initial mesh to model potential cracks. The initial mesh can consist of tetrahedrons, wedges, bricks or a mixture of these elements. Neither remeshing nor objective crack propagation criteria are needed. Four examples of concrete specimens, including a wedgesplitting test, a notched beam under torsion, a pull-out test of an anchored cylinder and a notched beam under impact, were modelled and analysed. The simulated crack propagation processes and load-displacement curves agreed well with test results or other numerical simulations for all the examples using initial meshes with reasonable densities. Making use of Abaqus＇s rich pre/post- processing functionalities and powerful standard/explicit solvers, the developed method offers a practical tool for engineering analysts to model complex 3D fracture problems.

关键词： finite element method cohesive elements three-dimensional crack propagation discrete crack model concrete structures Abaqus

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想去摸摸大海

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家教世界·创新阅读 2022年第10期 42-43页

作者： gq情感研究所

我是小马,天生全盲.十几年前,我从河北唐山的一片庄稼地走出来,到北京学了推拿,吃住在店里,不用出门.店里总共80平方米,员工宿舍8平方米,只能放下一张折叠床,走来走去都是墙,我不喜欢,老想走出店,呼吸新鲜空气,晒晒太阳.

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骨龄评估及其与运动的相关性

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中国组织工程研究与临床康复 2007年第45期11卷 9182-9185页

作者：李古强郭成吉徐州师范大学体育学院江苏省徐州市221116

目的:对骨龄的评价方法,运动对骨龄的作用及影响进行综述。资料来源:应用计算机检索维普资讯、CNKI数据库及手工检索1989-01/2007-02期间的相关文献。中文检索词包括"骨龄","运动","体育锻炼","青少年";英文检索词有"skeletal age","mov... 详细信息

目的:对骨龄的评价方法,运动对骨龄的作用及影响进行综述。资料来源:应用计算机检索维普资讯、CNKI数据库及手工检索1989-01/2007-02期间的相关文献。中文检索词包括"骨龄","运动","体育锻炼","青少年";英文检索词有"skeletal age","movement","physical training","bone age"。资料选择:共收集到相关文献397篇,阅读全部文章的文题和大部分文章的摘要。选择文献所述内容与运动对骨龄的作用及影响相关的文献。排除重复性研究。资料提炼:共得到符合纳入条件的文献129篇,排除268篇。选择其中32篇进行分析,英文17篇,中文15篇。资料综合:目前,国际上普遍采用G-P图谱法和TW2计分法评价骨龄,但这两种方法都不完全适合中国儿童青少年。在20世纪90年代,国家体委在TW2法的基础上提出了一套中国人的骨发育等级标准——CHN计分法。2006年,张绍岩等参照TW3方法,以百分位数法分别制订了TW3-C RUS、TW3-C腕骨、RUS-CHN骨龄标准,称为《中国人手腕骨发育标准-中华05》,在这种评价法中,虽然考虑到一些因素的影响,但其调查对象均为城市汉族正常青少儿,然而,诸多因素如种族、遗传、性别、疾病、营养、体育锻炼、情绪波动、生活习惯等,均可影响全身发育和内分泌器官机能,从而影响骨骼发育。此外,地理环境不同,居民饮食结构、质量及生活习惯等亦不同,进而会引起各地区人群发育的差异。结论:由于骨的发育会受到多因素的影响,有些因素还互相影响、互相干扰,因此,在实际工作中,应充分考虑到诸多因素对骨龄评价的影响;同时,由于骨龄标准的时限性,一般认为骨龄标准最好每10～15年修正一次;并制定本地区、本民族人群的骨龄标准。

关键词：运动骨龄年龄青少年体育锻炼

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维持性血液透析患者抑郁与社会支持、应对方式及心理防御机制的相关性

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中国组织工程研究与临床康复 2007年第52期11卷 10654-10657页

作者：翟永莉张桂青石河子大学医学院护理系石河子大学第一附属医院康复心理科新疆维吾尔自治区石河子市832008

目的:介绍维持性血液透析患者抑郁的发生状况,进一步了解维持性血液透析患者抑郁的发生与社会支持、应对方式、心理防御机制之间的关系。资料来源:应用计算机检索Medline数据库1993-02/2006-09与血液透析患者抑郁相关的文章,检索词为"ha... 详细信息

目的:介绍维持性血液透析患者抑郁的发生状况,进一步了解维持性血液透析患者抑郁的发生与社会支持、应对方式、心理防御机制之间的关系。资料来源:应用计算机检索Medline数据库1993-02/2006-09与血液透析患者抑郁相关的文章,检索词为"hameodialysis,mentalstate",限定文章语种为English。同时应用计算机检索中国期刊全文数据库1980/2006期间的相关文章,检索词为"血液透析,抑郁","血液透析,心理状况","血液透析,社会支持","抑郁,心理防御机制"。资料选择:纳入标准是血液透析患者与抑郁相关的文章,以实验研究类文章为主,同时包括综述性文章。资料提炼:共收集到92篇关于血液透析患者抑郁方面的研究文献,根据纳入标准,排除血液透析患者与抑郁关系不大的文章,以及与心理干预有关的文章,最后30篇文献纳入分析。资料综合:维持性血液透析患者抑郁的产生与社会支持呈显著负相关;维持性血液透析患者多采用消极的应对方式,抑郁的产生与负性应对方式高度正相关;抑郁患者的心理防御机制具有更多的不成熟防御方式和较少的成熟防御方式。结论:大部分维持性血液透析患者都存在不同程度的抑郁状态。良好的社会支持、积极的应对方式、成熟的心理防御机制对血液透析患者的身心健康具有直接的保护作用。

关键词：维持性血液透析抑郁社会支持应对方式心理防御机制

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Histopathological changes of Macaca mulatta infected with Plasmodium knowlesi

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Chinese Medical Journal 2001年第10期114卷 65-69,108页

作者：陈林李国桥卢耀增罗灼玲广州中医药大学热带医学研究所广州510405 广州中医药大学组织胚胎学教研室广州510405

Objective To study the histopathological changes of relevant intemal organs of Macaca mulatta infected with Plasmodium knowlesi ( P. knowlesi).Methods Histopathological examination of 3 monkeys who died of P. knowlesi infection, 2 P. knowlesi infected monkeys who died of treatment failure with artesunate suppository and 1 P. knowlesi infected monkey that was cured by piperaquine phosphate (PQP) but died of trauma and necrosis of the fore *** The heart, liver, spleen, lung, kidney, brain, pancreas, parathyriod, pituitary and lymph nodes showed severe pathological changes in 3 monkeys (No. 1, 7 and 12) who died of P. knowlesi infection and 1 infected monkey (No. 72) who died of treatment failure with artesunate suppository. Red blood cells containing rmalarial parasites and pigments were concentrated in the capillaries of these organs. Malarial pigments were deposited in many organs or phagocytized by macrophages in 1 monkey (No. 131 ), it was cured by piperaquine phosphate but died of trauma and necrosis of the fore limb; cellular atrophy and disappearance of pancreatic islets, parathyroid and pituitary cells were also observed. One monkey (No.33) treated with artesunate suppository, showed that blood parasites became negative but recrudesced and pituiary later died from a gavage accident. Its organs showed a significant difference to those of the infected monkeys receiving no treatment. Only the liver Kupffer cells and cerebral matrix contained malarial parasites and pigments; many relevant intemal organs showed *** The pathological changes of relevant internal organs of Macaca mulatta infected with P. knowlesi were examined in detail, especially cellular atrophy and the disappearance of pancreatic islets, parathyoid and pituitary cells and myolysis of cardiac muscles. These changes have not previously been reported elsewhere.

关键词： Plasmodium knowlesi · Macaca mulatta · necropsy · histopathology

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Efects of idle time on biological phosphorus removal by sequencing batch reactors

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Journal of Environmental Sciences 2013年第12期25卷 2396-2402页

作者： Dawen Gao Hang Yin Lin liu Xing Li Hong Liang School of Forestry Northeast Forestry University State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Institute of Urban Environment Chinese Academy of Sciences

Three identical sequencing batch reactors （SBRs） were operated to investigate the effects of various idle times on the biological phosphorus （P） removal. The idle times were set to 3 hr （R1）, 10 hr （R2） and 17 hr （R3）. The results showed that the idle time of a SBR had potential impact on biological phosphorus removal, especially when the influent phosphorus concentration increased. The phosphorus removal efficiencies of the R2 and R3 systems declined dramatically compared with the stable R1 system, and the Prelease and P-uptake rates of the R3 system in particular decreased dramatically. The PCR-DGGE analysis showed that uncultured Pseudomonas sp. （gq183242.1） and β-Proteobacteria （AY823971） were the dominant phosphorus removal bacteria for the R1 and R2 systems, while uncultured γ-Proteobacteria were the dominant phosphorus removal bacteria for the R3 system. Glycogen-accumulating organisms （GAOs）, such as uncultured Sphingomonas sp. （AM889077）, were found in the R2 and R3 systems. Overall, the R1 system was the most stable and exhibited the best phosphorus removal efficiency. It was found that although the idle time can be prolonged to allow the formation of intracellular polymers when the phosphorus concentration of the influent is low, systems with a long idle time can become unstable when the influent phosphorus concentration is increased.

关键词： biological phosphorus removal polyphosphate-accumulating organisms idle time microbial community

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