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Combinations of Hd2 and Hd4 genes determine rice adaptability to Heilongjiang Province, northern limit of China

Journal of Integrative Plant Biology 2015年第8期57卷 698-707页

作者： Xiufeng Li Huazhao liu Maoqing Wang hualong liu Xiaojie Tian Wenjia Zhou Tianxiao Lü Zhenyu Wang Chengcai Chu Jun Fang Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breeding the Chinese Academy of Sciences University of Chinese Academy of Sciences Rice Research Institute Heilongjiang Academy of Land Reclamation Sciences Beidahuang Kenfeng Seed Co.Ltd. College of Agriculture Northeast Agricultural University State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Heading date is a key trait in rice domestication and adaption, and a number of quantitative trait loci（QTLs）have been identified. The rice（Oryza sativa L.） cultivars in the Heilongjiang Province, the northernmost region of China,have to flower extremely early to fulfill their life ***, the critical genes or different gene combinations controlling early flowering in this region have not been determined. QTL and candidate gene analysis revealed that Hd2/Ghd7.1/Os PRR37 plays a major role in controlling rice distribution in Heilongjiang. Further association analysis with a collection of rice cultivars demonstrated that another three major QTL genes（Hd4/Ghd7, Hd5/DTH8/Ghd8, and Hd1）also participate in regulating heading date under natural long day（LD） conditions. Hd2/Ghd7.1/Os PRR37 and Hd4/Ghd7 are two major QTLs and function additively. With the northward rice cultivation, the Hd2/Ghd7.1/Os PRR37 and Hd4/Ghd7 haplotypes became non-functional alleles. Hd1 might be non-functional in most Heilongjiang rice varieties,implying that recessive hd1 were selected during local rice breeding. Non-functional Hd5/DTH8/Ghd8 is very rare, but constitutes a potential target for breeding extremely early flowering cultivars. Our results indicated that diverse genetic combinations of Hd1, Hd2, Hd4, and Hd5 determined the different distribution of rice varieties in this northernmost province of China.

关键词： Rice heading date Hdl Hd2 Hd4 Hd5

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Elite sd1 alleles in japonica rice and their breeding applications in northeast China

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The Crop Journal 2022年第1期10卷 224-233页

作者： Hanjing Sha hualong liu Guangxin Zhao Zhongmin Han Huilin Chang Jingguo Wang Hongliang Zheng Jifeng Zhang Yang Yu Yuqiang liu Detang Zou Shoujun Nie Jun Fang Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina Innovation Center Suihua Branch of Heilongjiang Academy of Agricultural SciencesSuihua 152052HeilongjiangChina University of Chinese Academy of Sciences Beijing 100049China College of Life Science and Engineering Shenyang UniversityShenyang 110044LiaoningChina The Innovative Academy for Seed Design Chinese Academy of SciencesBeijing 100101China

The Green Revolution gene sd1 has been used extensively in modern rice breeding,especially in indica ***,elite sd1 alleles and related germplasm resources used for japonica rice breeding have not been identified,and extensive efforts are needed for japonica rice breeding to obtain new dwarfing *** from MBKbase-Rice revealed seven sd1 haplotypes in indica and four in japonica *** new sd1 alleles were identified in indica *** 295 japonica accessions from northeast Asia,except for the weak functional allele SD1-EQ,sd1-r was the major allele,reducing plant height in comparison with *** germplasm resources carrying reported sd1 alleles were identified by genotype searching and further verified by literature search,genealogical analysis,and d Caps *** and geographic distribution showed that sd1-r is an excellent allele widely used in northern China and Tohoku in Japan,and sd1-j is commonly used in east China and Kyushu in ***-and Xiushui-series cultivars carrying sd1-r and sd1-j,respectively,are essential branches of the backbone parents of Chinese japonica rice,Akihikari and Ce21,with the largest number of descendants and derived *** semi-dwarf japonica rice breeding,sd1-d was introgressed into Daohuaxiang 2(DHX2).Dwarf and semi-dwarf lines carrying sd1-d were selected and designated as 1279 and 1280,respectively,after withstanding typhoon-induced strong winds and heavy rains in 2020,and are anticipated to become useful intermediate materials for future genetic research and *** work will facilitate the introduction,parental selection,and marker-assisted breeding,and provide a material basis for the next step in identifying favorable genes that selected together with the sd1 alleles in japonica backbone parents.

关键词： Semi-dwarf breeding Elite allele Pedigree analysis Geographic distribution Regional adaptability

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High-Efficiency Reduction of Rice Amylose Content via CRISPR/Cas9-Mediated Base Editing

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Rice science 2020年第6期27卷 445-448页

作者： Li He Li Xiufeng Xu Yang liu hualong He Mingliang Tian Xiaojie Wang Zhenyu Wu Xiuju Bu Qingyun Yang Jie College of Life Science Northeast Agricultural UniversityHarbin 150030China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingChinese Academy of SciencesHarbin 150081China Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014China Rice Research Institute College of AgricultureNortheast Agricultural UniversityHarbin 150030China

Rice is a staple food for more than half of the human *** has been estimated that by 2030,rice production must increase by 40%to meet the growing demand(Khush,2005).In addition,with the improvement of people's living standards,the demand for elite rice with better eating and cooking quality(ECQ)is *** is determined by several factors,including amylose content(AC),gel consistency(GC),gelatinization temperature(GT)and viscosity,where AC is the predominant factor(Juliano,1998).

关键词： CRISPR/Cas9 elite cooking

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Study on Difference in Cold Tolerance Between Weedy Rice and Cultivated Rice in Heilongjiang Province of China

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Journal of Northeast Agricultural University(English Edition) 2008年第1期15卷 1-7页

作者： ZOU Detang GUO Junxiang liu hualong WANG Jingguo College of Agriculture Northeast Agricultural University Harbin 150030 China

A long-red awn weedy rice and rice cultivars named Ken99004（ZCI）, Crossing-503（ZC2）, Ken Sticky Rice（ZC3）, Shashani（ZC4）, Long-Grain Aromatic Rice（ZC5） were used to study typical wild traits of weedy rice and to identify the cold resistance of all genotypes. The results showed that the stem of long-red awn weedy rice was the weakest. No difference was found in the early stage of rice growth, e.g. the vegetable growth stage, between long-red awn weedy rice and other rice cultivars in the stem rigidity, but in the later stage, or reproductive growth stage, their leaves and stems were senescent rapidly because of the speedy supply of nutrients for panicle growth, meanwhile the rigidity of stem was reduced sharply just as withered weeds. The germination rate of long- red awn weedy rice was the highest in cold condition, and in turn were ZCI, ZC2, ZC3, ZC4, ZC5, respectively. The performances of genotypes in cold tolerance were identical, those with a high ability of germination in low temperature also showed a strong cold tolerance in main traits in whole growth period, the order from strong to weak in the extent of cold tolerance were long-red awn weedy rice, ZCl, ZC2, ZC3, ZC4, ZC5, respectively

关键词： weedy rice genotype cold tolerance

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序列二次规划-遗传算法及其在变压器局部放电超声定位中的应用

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电网技术 2015年第1期39卷 130-135页

作者：刘化龙胡钋武汉大学电气工程学院湖北省武汉市430072

针对基本遗传算法(genetic algorithm,GA)局部寻优能力较差和易出现早熟收敛现象,提出了一种改进的遗传算法,即序列二次规划-遗传算法(sequential quadratic programminggenetic algorithm,SQP-GA)。该混合优化算法SQP-GA在基本GA中引... 详细信息

针对基本遗传算法(genetic algorithm,GA)局部寻优能力较差和易出现早熟收敛现象,提出了一种改进的遗传算法,即序列二次规划-遗传算法(sequential quadratic programminggenetic algorithm,SQP-GA)。该混合优化算法SQP-GA在基本GA中引入序列二次规划(sequential quadratic programming,SQP)算法,经典算法SQP作为一个元算子有效地提高了基本GA的局部搜索能力,并克服了早熟收敛现象。函数仿真测试结果表明,SQP-GA混合优化算法在收敛速度和寻优精度上都优于基本GA,表明所提出的算法的有效性。同时,利用提出的SQP-GA求解变压器局部放电超声波定位问题,并将其定位结果与GA和非智能算法的定位结果进行比较;算例结果表明,基于SQP-GA的变压器局部放电超声定位法能有效地防止结果陷入局部最优,该方法的定位效果理想。

关键词：遗传算法序列二次规划-遗传算法变压器局部放电超声波定位非智能算法

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OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice(Oryza sativa L.)

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The Crop Journal 2021年第5期9卷 1003-1017页

作者： Jiaming Li Minghui Zhang Luomiao Yang Xinrui Mao Jinjie Li Lu Li Jingguo Wang hualong liu Hongliang Zheng Zichao Li Hongwei Zhao Xianwei Li Lei Lei Jian Sun Detang Zou Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina College of Life Science Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement China Agricultural UniversityBeijing 100094China

The C (Cys) 2H (His) 2-type transcription factor is one of the most important transcription factors in plants and plays a regulatory role in the physiological responses of rice to abiotic stresses.A novel rice C2H2-type zinc finger protein,abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3),was found to confer drought stress tolerance by enhancing antioxidant defense and regulating Os *** of OsADR3 in rice increased tolerance to drought stress by increasing ROS scavenging ability and ABA *** contrast,CRISPR/Cas9-mediated knockout of osadr3 increased the sensitivity of rice to drought and oxidative *** exogenous ROS-scavenging reagent restored the droughtstress tolerance of osadr3-CRISPR *** transcriptome analysis suggested that OsADR3 increased the expression of Os GPX1 under drought *** mobility shift,yeast one-hybrid,and dualluciferase reporter assays revealed that OsADR3 modified the expression of Os GPX1 by directly binding to its *** of Os GPX1 repressed ROS scavenging ability under drought stress in OsADR3-overexpression *** findings suggest that OsADR3 plays a positive regulatory role in droughtstress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice.

关键词： Drought stress Rice ROS scavenging Glutathione peroxidase Abscisic acid

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Identification of Cold Tolerance of Rice Germplasm Resource at Germinating Stage in Cold Region

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Journal of Northeast Agricultural University(English Edition) 2008年第2期15卷 1-4页

作者： liu hualong SUN Shiche WANG Jingguo ZOU Detang College of Agriclture Northeast Agricultural University Harbin 150030 China Heilongjiang Academy of Agricultural Sciences Harbin 150086 China

Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance. The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant （HT）, tolerant （T）, moderately tolerant （MT）, susceptible （S）, highly susceptible （HS）.

关键词： rice cold tolerance germplasm resource identification

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贵州某高硫铝土矿焙烧脱硫后的预脱硅和沉降性能研究

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湿法冶金 2015年第4期34卷 308-311页

作者：刘华龙中电投贵州遵义产业发展有限公司务川氧化铝分公司贵州遵义563100

研究了贵州某高硫铝土矿焙烧后的预脱硅和矿浆沉降性能。结果表明:随焙烧时间延长和温度升高,预脱硅率升高;随矿浆固含量增加,预脱硅率降低;石灰添加量对预脱硅影响不大;随苛性碱质量浓度增大,预脱硅率升高;在苛性碱质量浓度248g/L、石... 详细信息

研究了贵州某高硫铝土矿焙烧后的预脱硅和矿浆沉降性能。结果表明:随焙烧时间延长和温度升高,预脱硅率升高;随矿浆固含量增加,预脱硅率降低;石灰添加量对预脱硅影响不大;随苛性碱质量浓度增大,预脱硅率升高;在苛性碱质量浓度248g/L、石灰添加量9%、反应时间8h、温度100℃、矿浆固含量300g/L适宜条件下,预脱硅率为64.5%左右;稀释矿浆苛性碱质量浓度为160g/L,矿浆沉降速度10m/h,矿浆固含量以70g/L为宜,絮凝剂添加量为80g/t左右。

关键词：铝土矿硫预脱硅焙烧沉降性能

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抗病基因Pi-ta在粳稻骨干亲本及其衍生品种中的传递

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东北农业大学学报 2013年第7期44卷 27-32页

作者：刘化龙刘华招东北农业大学农学院哈尔滨150030 黑龙江省农垦科学院水稻研究所黑龙江佳木斯154007

利用Pi-ta显性分子标记对寒地稻区水稻骨干亲本合江20号及其衍生品种进行Pi-ta抗性基因传递分析。结果表明,抗病基因Pi-ta在亲本合江20号衍生一代出现频率为63%,衍生二代出现频率为33%,衍生三代出现频率为9%,抗病基因Pi-ta传递与合江20... 详细信息

利用Pi-ta显性分子标记对寒地稻区水稻骨干亲本合江20号及其衍生品种进行Pi-ta抗性基因传递分析。结果表明,抗病基因Pi-ta在亲本合江20号衍生一代出现频率为63%,衍生二代出现频率为33%,衍生三代出现频率为9%,抗病基因Pi-ta传递与合江20号衍生系谱一致。抗谱分析表明,Pi-ta抗病基因在衍生代中出现与衍生品种的抗谱呈正相关,与后代选择过程中抗病基因丢失有关,是决定不同水稻品种稻瘟病发生程度的主要原因之一。

关键词： Pi-ta基因骨干亲本合江20号基因传递

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自分级挤压式核桃破壳机关键部件作业参数优化与试验

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中国农机化学报 2024年第4期45卷 85-92页

作者：刘华龙曾勇张宏塔里木大学机械电气化工程学院新疆阿拉尔843300 新疆维吾尔自治区教育厅普通高等学校现代农业工程重点实验室新疆阿拉尔843300

通过分析不同作业参数对自分级挤压式核桃破壳机破壳性能的影响规律,优化关键部件的作业参数,提高破壳性能。以核桃在辊板间的运动学分析为基础,确定影响核桃破壳取仁的关键因素为破壳角度和轧辊转速,并以破壳率、整仁率和能耗为试验指... 详细信息

通过分析不同作业参数对自分级挤压式核桃破壳机破壳性能的影响规律,优化关键部件的作业参数,提高破壳性能。以核桃在辊板间的运动学分析为基础,确定影响核桃破壳取仁的关键因素为破壳角度和轧辊转速,并以破壳率、整仁率和能耗为试验指标,开展两因素五水平二次正交旋转中心组合试验。利用Design-Expert软件进行数据分析,建立各因素和试验指标之间的回归模型并进行方差分析,得到影响破壳率、整仁率和能耗的主次因素。利用响应面法分析两个因素的交互作用,根据优化目标的重要程度对回归模型进行多目标优化。试验结果表明:最优工作参数组合为破壳角度0.47°,轧辊转速108 r/min,在此条件下,得出破壳率为97.24%,整仁率为92.03%,能耗为1.88 kJ/kg,验证试验结果与模型预测值相对误差小于5%。品种适应性试验结果表明:最优工作参数对3个品种核桃(温185、新温179、扎343)的适应性较佳,表现为破壳率均大于96%,整仁率均大于90%,能耗均小于2 kJ/kg。为相关破壳取仁设备改进与优化以及不同品种核桃破壳加工参数的选取提供理论参考。

关键词：核桃破壳机参数优化响应面法

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