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Antibacterial and antibiofi lm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags

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Food Science and Human Wellness 2022年第6期11卷 1607-1613页

作者： Shen Yang Zijin Yuan Jude Juventus Aweya Shanggui Deng Wuyin Weng Yueling Zhang guangming liu College of Food and Biological Engineering Xiamen Key Laboratory of Marine Functional FoodJimei UniversityXiamen 361021China Department of Biology Guangdong Provincial Key Laboratory of Marine BiotechnologyShantou UniversityShantou 515063China College of Food and Pharmacy Zhejiang Ocean UniversityZhoushan 316000China

Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food *** this study,5 species of bacteria,mainly gram-positive bacteria including three species of Bacillus spp.,Arthrobacter arilaitensis and Staphylococcus warneri,were isolated and identifi ed from bacteria-contaminated *** culture *** in silico predicted antimicrobial peptide from theβ-1,3-glucan-binding protein sequence of Penaeus vannamei,designated Pv GBP2(FLKLGRKSRYGMLKL),was screened and its antibacterial effect and mechanism of action on the isolated Bacillus *** minimal inhibitory concentrations(MIC)of Pv GBP2 on Bacillus *** 15.6–31.25μg/m *** Pv GBP2 could inhibit Bacillus subtilis in *** culture bags to maintain growth and yield of *** electron microscopy(TEM)revealed that Pv GBP2 kills bacteria by perforating the cell wall,destroying membrane integrity and resulting in the leakage of intracellular *** addition,Pv GBP2 inhibits biofi lm formation by *** by 90.6%at 1×***,peptide Pv GBP2 could be potentially applied as an antibacterial agent to control bacterial infection of *** cultivation and the spread of foodborne pathogens.

关键词： Auricularia auricular Antimicrobial peptide Penaeus vannamei β-1,3-Glucan-binding protein Bacillus spp.e

Mechanical properties and interfacial characteristics of 6061 Al alloy plates fabricated by hot-roll bonding

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International Journal of Minerals,Metallurgy and Materials 2024年第8期31卷 1890-1899页

作者： Zongan Luo Xin Zhang Zhaosong liu Hongyu Zhou Mingkun Wang guangming Xie State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China

This work aims to investigate the mechanical properties and interfacial characteristics of 6061 Al alloy plates fabricated by hotroll bonding(HRB)based on friction stir *** results showed that ultimate tensile strength and total elongation of the hot-rolled and aged joints increased with the packaging vacuum,and the tensile specimens fractured at the matrix after exceeding 1 ***-equilibrium grain boundaries were formed at the hot-rolled interface,and a large amount of Mg_(2)Si particles were linearly precipitated along the interfacial grain boundaries(IGBs).During subsequent heat treatment,Mg_(2)Si particles dissolved back into the matrix,and Al_(2)O_(3) film remaining at the interface eventually evolved into *** addition,the local IGBs underwent staged elimination during HRB,which facilitated the interface healing due to the fusion of grains at the *** process was achieved by the dissociation,emission,and annihilation of dislocations on the IGBs.

关键词： 6061 Al alloy hot-roll bonding vacuum mechanical properties interfacial grain boundaries

Cerium Oxide Film Prepared from Molten Salts

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Rare Metals 2001年第2期20卷 95-100页

作者： Xindong Wang, guangming liu, Yanfang liu, Shuzhen Duan Department of Physical Chemistry, University of Science and Technology Beliing, Beliing 100083. China) Univ Sci & Technol Beijing Dept Chem Phys Beijing 100083 Peoples R China

Cerium oxide film formed on the nickel electrode at 453 K in LiNO3-KNO3 molten salts. The molten salt background and the cathode process of Leeds on the nickel electrode were studied by using cyclic voltammetry. The cathodic reaction mechanism of the molten salt background was: O-2(-)+e O-2(-2); O-2+2NO(3)(-) --> 2NO(2)(-)+2O(2). According to the results of SEM and XPS, cerium oxide film, which is composed of CeO2, can form on the nickel electrode at - 1.850 V vs. Ag/AgNO3 (0.1 mol/L) reference electrode.

关键词： cerium oxide low-temperature molten salts film

Successive digestion of tilapia collagen by serine proteinase and proline specific endopeptidase to produce novel angiotensin l-converting enzyme inhibitory peptides

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Marine Life Science & Technology 2020年第3期2卷 268-278页

作者： Xin Hua Lechang Sun Chan Zhong Qiang Wu Panpan Xiao Asami Yoshida guangming liu Minjie Cao College of Food and Biological Engineering Jimei UniversityXiamen 361021China Collaborative Innovation Center of Marine Food Deep Processing Dalian Polytechnic UniversityDalian 116034China Department of Biological and Chemical Engineering Shaoyang UniversityShaoyang 422000China Faculty of Fisheries Nagasaki UniversityNagasaki 852-8521Japan

Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angiotensin I-converting enzyme(ACE)inhibitory peptides(ACEIPs).A series of column chromatography assays were used to separate the ACEIPs.A peptide,NPARTCR,was isolated as it exhibited high ACE inhibition *** digestion of this peptide by a proline specific endopeptidase(PSEP),produced a pentapeptide ARTCR with ACE inhibitory activity(IC_(50))of 77.0 pmol/*** NPARTCR and ARTCR inhibited ACE in a non-competitive *** in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats(SHRs).These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen,*** proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension.

关键词： Serine proteinase Purification Collagen ACE inhibitory peptides Hypertension Lowering blood pressure

Cerium-tannic acid passivation treatment on galvanized steel

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Rare Metals 2009年第3期28卷 284-288页

作者： liu guangming YU Fei YANG liu TIAN Jihong DU Nan Key Laboratory of Nondestructive Testing （Nanchang Hangkong University） Ministry of Education of China Nanchang 330063 China School of Aeronautic and Mechanical Engineering Nanchang Hangkong University Nanchang 330063 China

A novel cerium-tannic acid passivation treatment was performed on galvanized steel. The corrosion resistance of cerium-tannic passivated samples was tested by dropping test with 0.5 wt.% CuSO4 aqueous solution. The mass loss per unit area of passivated samples was measured after the corrosion in 0.5 mol/L NaCl ＋ 0.005 mol/L H2SO4 at room temperature for 96 h. The electrochemical behaviors of cerium, tannic acid, and cerium-tannic acid passivated samples on galvanized steel in 0.5 mol/L NaCl solution were investigated by polarization curves and electrochemical impendence spectra. The corrosion equivalent circuit was established according to the impedance characteristics. The results show that cerium-tannic acid treated samples exhibit better corrosion resistance than the sole cerium or tannic acid treated samples under the same condition. The mechanism of synergistic effect for cerium-tannic acid passivation on galvanized steel was discussed.

关键词： passivation cerium tannic acid galvanized steel corrosion

Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus ***1

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Acta Oceanologica Sinica 2011年第6期30卷 95-103页

作者： ZHU Yanbing LI Hebin ZHANG Xuqin ZHANG Chunyan XIANG Jionghua liu guangming School of Biotechnology Engineering Jimei University Xiamen 361021 China Xiamen Medical College Xiamen 361008 China

A thermostable superoxide dismutase （SOD） from the inshore thermophile Thermus sp. JM1 was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1 656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli （E. coli）. The prepared apo-enzyme of the purified recombinant SOD （rSOD） was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2＋ - reconstituted rSOD （Mn-rSOD） exhibited the specific activity of 1 598 U/mg. SOD from Thermus sp. JM1 was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 11.0.

关键词： manganese superoxide dismutase thermostability purification reconstitution

一流课程建设背景下塑性力学与轧制原理课程教学改革与实践

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高教学刊 2024年第14期10卷 27-30,34页

作者：杨霞马立峰刘光明牛勇李华英太原科技大学太原030024

在“双万计划”一流课程建设背景下,该文从塑性力学与轧制原理的课程特点和教学现状出发,提出两向贯通、三位一体、四维联动、五步递进和多元融合的教学理念。即以学生为主体,将塑性力学与轧制原理两部分知识内容进行融会贯通,并依据教... 详细信息

在“双万计划”一流课程建设背景下,该文从塑性力学与轧制原理的课程特点和教学现状出发,提出两向贯通、三位一体、四维联动、五步递进和多元融合的教学理念。即以学生为主体,将塑性力学与轧制原理两部分知识内容进行融会贯通,并依据教学目标定位,进行课程教学内容重构,促进教学方法创新,完善教学评价体系。通过四个维度的相互支撑,将知识传授、能力培养和价值塑造三个教学目标融为一体,在教学实践过程中采用五步递进式的教学方法,实行政教融合、科教融合、产教融合及赛教融合,形成教育合力,实现对学生各项能力的培养。新的教学模式实践表明,教学效果明显提高,可以为塑性力学与轧制原理课程教学改革提供新思路。

关键词：一流课程塑性力学与轧制原理教学理念教学改革教学实践

生物防治与化学防治协同处理防治柑橘全爪螨的温室试验

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植物医学 2024年第2期3卷 20-29页

作者：安若飞余金珂王进军豆威汪昭军刘光铭西南大学植物保护学院重庆400715 重庆潼南柠檬科技小院重庆402660 重庆市潼南区农业科技推广中心重庆402660

通过对在重庆市潼南区柏梓镇潼南柠檬脱毒种苗繁育中心的4号大棚中设置的6个处理,在化学防治的基础上选择用药后的不同时间释放捕食螨,以探究生-化协同防治柑橘全爪螨的效果.试验结果表明,施用阿维菌素后第6 d释放捕食螨对柑橘全爪螨的... 详细信息

通过对在重庆市潼南区柏梓镇潼南柠檬脱毒种苗繁育中心的4号大棚中设置的6个处理,在化学防治的基础上选择用药后的不同时间释放捕食螨,以探究生-化协同防治柑橘全爪螨的效果.试验结果表明,施用阿维菌素后第6 d释放捕食螨对柑橘全爪螨的防效最好,虫口减退率和防治效果均可以达到98%以上;且相较于单一施用化学农药持效期更长,约为1.5月,而单一施用化学农药持效期只能维持在10 d左右.由气象因子数据和柑橘全爪螨发生动态相关性分析结果可知,施用化学农药后可提高柑橘全爪螨种群对高温的抗逆性;施用阿维菌素后第6 d释放捕食螨天气积分指数对柑橘全爪螨的影响最大,说明微气候也会对柑橘全爪螨的防治产生影响.研究结果为柠檬产业害螨绿色防治提供了更精细化的方案.

关键词：柑橘全爪螨化学防治生物防治捕食螨气象因子天气积分指数

渤海油田新近系地层剖面智能化建立方法及应用

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录井工程 2024年第1期35卷 22-26页

作者：李战奎张恒张学斌温海涛刘广明中海油能源发展股份有限公司工程技术分公司

随着钻井作业优快发展,新近系浅层探井钻井作业周期大幅缩短,导致砂泥岩难区分,岩性分层不准确,录井剖面符合率低。为解决这项难题,实现新近系地层岩性剖面的准确构建,提出基于钻压、扭矩、钻时的组合参数SI指数与扭矩曲线交会方法实时... 详细信息

随着钻井作业优快发展,新近系浅层探井钻井作业周期大幅缩短,导致砂泥岩难区分,岩性分层不准确,录井剖面符合率低。为解决这项难题,实现新近系地层岩性剖面的准确构建,提出基于钻压、扭矩、钻时的组合参数SI指数与扭矩曲线交会方法实时构建录井剖面,使录井剖面绘制可以随钻实时进行,做到了录井剖面绘制及时、高效,实现了精细化岩性分层。该方法在渤海油田应用超100口井,地质录井剖面符合率提升15%~23%,为智能化录井奠定了基础。

关键词：新近系智能化地层剖面 SI指数符合率岩性识别