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不同浓度微量元素处理对斑兰叶组培过程中挥发性物质及角鲨烯相关酶活性的影响

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热带作物学报 2024年第7期45卷 1455-1466页

作者：林群原闫露吉训志马兆成郑日如秦晓威胡丽松华中农业大学园艺林学学院湖北武汉430070 中国热带农业科学院香料饮料研究所海南万宁571533 海南省热带香辛饮料作物遗传改良与品质调控重点实验室海南万宁571533 农业农村部香辛饮料作物遗传资源利用重点实验室海南万宁571533

为了研究不同浓度微量元素处理组培斑兰叶的挥发性物质及角鲨烯相关酶活性的变化,本研究以不同浓度锌(Zn)、镧(La)元素处理的组培斑兰叶为原料,采用GC-MS技术对其挥发性物质进行分析,并测定角鲨烯代谢关键酶活性。结果表明:不同浓度微... 详细信息

为了研究不同浓度微量元素处理组培斑兰叶的挥发性物质及角鲨烯相关酶活性的变化,本研究以不同浓度锌(Zn)、镧(La)元素处理的组培斑兰叶为原料,采用GC-MS技术对其挥发性物质进行分析,并测定角鲨烯代谢关键酶活性。结果表明:不同浓度微量元素处理的组培斑兰叶挥发性物质的数量、含量均存在差异,与未处理的组培斑兰叶相比,4 mg/LZn元素处理和1、2、4 mg/LLa元素处理的组培斑兰叶中的挥发性物质较多。Zn、La元素处理均使组培斑兰叶中的醇类物质含量增加、烯烃类物质含量减少;Zn、La元素处理均使组培斑兰叶中的角鲨烯含量明显减少,2 mg/LZn元素处理使组培斑兰叶中的β-谷甾醇、豆甾醇、棕榈酸乙酯、3-甲基-2-(5H)-呋喃酮、2,3-二氢-3,5二羟基-6-甲基-4(h)-吡喃-4-酮含量增加,角鲨烯和叶绿醇含量减少;8 mg/L La元素处理组培斑兰叶的叶绿醇含量最高。角鲨烯代谢相关酶活性在组培斑兰叶培养过程中有一定变化,羊毛甾醇合成酶(LSS)和鲨烯环氧酶(SE)在混合物(愈伤组织开始分化出芽)中活性较强,角鲨烯合成酶(SQS)在不定根中活性较强。不同浓度微量元素处理对角鲨烯相关酶活性有一定影响,不同浓度Zn元素处理的组培斑兰叶中角鲨烯含量与LSS、SE、SQS酶活性均呈正相关;La元素处理组培斑兰叶中角鲨烯含量与LSS、SQS酶活性呈负相关,与SE酶活性呈正相关。研究结果丰富了微量元素调控斑兰叶挥发性物质的相关理论研究,为挖掘提高斑兰叶香气品质的组培新技术提供理论依据。

关键词：斑兰叶锌元素镧元素挥发性物质酶活性

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区域性地质灾害综合防治系统研究

测绘与空间地理信息 2024年第8期47卷 68-72页

作者：王帅胡立嵩吴悠悠王美冷朝阳武汉工程大学资源与安全工程学院湖北武汉430073

为了进一步解决我国区域性地质灾害防治工作中,因环节信息化技术水平低而导致的区域性地质灾害信息不能发挥最大作用的问题,本文依托鄂州市“四位一体、网格化管理”的地质灾害防治体系,结合当前先进的物联网、大数据、可视化、无人机... 详细信息

为了进一步解决我国区域性地质灾害防治工作中,因环节信息化技术水平低而导致的区域性地质灾害信息不能发挥最大作用的问题,本文依托鄂州市“四位一体、网格化管理”的地质灾害防治体系,结合当前先进的物联网、大数据、可视化、无人机等技术,研究并建立了区域性地质灾害综合防治系统,明确了综合防治业务流程。结果表明该系统能实现对地质灾害各环节信息的数据收集、分析处理、可视化展示,能够提升自然资源、应急管理等部门的地灾防治工作效率,增强区域地质灾害防治能力。

关键词：区域性地质灾害综合防治

基于SPA的涂料企业重大危险源安全风险评价

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武汉工程大学学报 2024年第1期46卷 97-104页

作者：冷朝阳董万强胡立嵩王美王帅吴悠悠武汉工程大学资源与安全工程学院湖北武汉430074 武汉渠清工程咨询有限公司湖北武汉430074

提出了一种针对涂料企业重大危险源安全风险等级的评价模型,从固有危险因子和危险抵消因子两个方面,构建了重大危险源评价指标体系,采用组合赋权判断各级指标权重,通过集对分析法(SPA)建立联系数函数,求出各指标单项联系数和整体联系数... 详细信息

提出了一种针对涂料企业重大危险源安全风险等级的评价模型,从固有危险因子和危险抵消因子两个方面,构建了重大危险源评价指标体系,采用组合赋权判断各级指标权重,通过集对分析法(SPA)建立联系数函数,求出各指标单项联系数和整体联系数,以此为依据判定重大危险源的安全风险等级。结果表明:此模型可有效地解决评价中存在的重大危险源定量困难的难题,评价结果既能对企业安全状况作出分析,又能对企业未来的安全趋势作出合理的预测,可以为重大危险源的安全监管和事故预防工作提供理论支持。

关键词：集对分析组合赋权重大危险源联系数

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42份海南咖啡种质资源品质性状鉴定分析

分子植物育种 2024年第3期22卷 871-880页

作者：何安乐黄丽芳胡丽松庞永青王晓阳伍宝朵董云萍闫林李学俊中国热带农业科学院香料饮料研究所农业部香辛饮料作物遗传资源利用重点实验室海南省热带香辛饮料作物遗传改良与品质调控重点实验室海南省院士工作站万宁571533 云南农业大学热带作物学院普洱665000

为解决中国优质咖啡资源缺少的问题,本研究以海南42份咖啡资源为试验材料,通过超声超离法、杜马斯定氮仪燃烧法和UPLC法测定11个品质性状的含量,并进行多样性、相关性、主成分和层次聚类分析。研究结果显示,11个品质性状的变异系数范围... 详细信息

为解决中国优质咖啡资源缺少的问题,本研究以海南42份咖啡资源为试验材料,通过超声超离法、杜马斯定氮仪燃烧法和UPLC法测定11个品质性状的含量,并进行多样性、相关性、主成分和层次聚类分析。研究结果显示,11个品质性状的变异系数范围为7.42%~29.63%,4-阿魏酰奎宁酸(29.63%)最大,蛋白质(7.42%)最小。遗传多样性指数范围为1.27~2.05,最高的是蛋白质和葫芦巴碱(2.05),最低的是异绿原酸B(1.27),遗传多样性丰富。42份资源间品质性状存在相关性,脂肪与咖啡因和异绿原酸A呈显著负相关(P<0.05),咖啡因与7个性状呈显著正相关(P<0.001),7个绿原酸间均呈显著正相关(P<0.01)。主成分分析结果显示,前3个主成分贡献率达75.32%。综合表现排名前5的特异性种质分别为32号(5.63)、16号(2.94)、30号(1.10)、6号(0.80)和10号(0.74)。层次聚类分析中42份种质聚成4个类群,32号种质独占一类。42份咖啡种质资源品质存在差异,在选育种的时候需通过多种技术手段结合不同种质的优异性状,从而得到优异的咖啡种质资源。可优先选择综合排名靠前的咖啡种质作为选育和改良的基础材料。本研究对42份咖啡种质资源的11个品质性状进行鉴定评价,筛选特异的种质资源,为咖啡选育种提供试验材料,也为咖啡鉴定评价提供理论基础。

关键词：咖啡品质性状咖啡因绿原酸

A near-complete genome assembly of Catharanthus roseus and insights into its vinblastine biosynthesis and high susceptibility to the huanglongbing pathogen

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Plant Communications 2023年第6期4卷 216-220页

作者： Zhongping Xu Guanying Wang Qiongqiong Wang Xiaoting Li Guangyu Zhang Ali Qurban Can Zhang Yi Zhou huan Si lisong hu Fuqiu Wang Ying Wang Zhitao Tian Wei Chen Shuangxia Jin Fang Ding hubei Key Laboratory of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubeiChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanHubeiChina Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdaoShandongChina Spice and Beverage Research Institute Chinese Academy of Tropical Agricultural SciencesWanningHainanChina National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhanHubeiChina Key Laboratory of Horticultural Plant Biology of MOE(Ministry of Education) Huazhong Agricultural UniversityWuhanHubeiChina

Dear Editor,Catharanthus roseus,an important medicinal plant native to Madagascar,is the sole source of the two important anticancer drugs vinblastine and vincristine,which are synthesized by the monoterpene-derived indole alkaloid(MIA)pathway(Das et al.,2020).It is also an important model plant for the study of huanglongbing(HLB),a devastating citrus disease that causes severe economic losses worldwide(Ma et al.,2022).Vincristine is an FDA-approved drug that has been used for acute lymphocytic leukemia,lymphoid blast crisis of chronic myeloid leukemia,and Hodgkin’s/non-Hodgkin’s lymphoma(Below and M Das,2021).

关键词： drugs lymphocytic

Coating of Mineral Acids with Niobic Solid Acid for Preparing Furfural from Nut Shell of Camellia oleifera Abel

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Open Journal of Forestry 2016年第5期6卷 486-497页

作者： lisong hu Menghao Du Jingping Zhang Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang China

Nut shell of Camellia oleifera Abel which has large scale of plantation in mountainous region of southern China is abundant renewable resource. The nut shell is suitable for preparation of furfural, as the content of which is as much as 16% (based the dried nut shell). In early time, mineral acids were employed as typically catalyst for preparing of furfural from the nut shells. These mineral acids could pollute water and corrode equipment. In this paper we used various mineral acids coating with niobic acid as catalysts to investigate reactions for preparation of furfural. Among these catalysts, the catalyst of sulfuric acid coating with niobic acid was found to be very effective, which had higher hammett acidity and better effect of hydrolysis of the nut shells;The catalysts of sulfuric acid coating with niobic acid was characterized, and the conditions of preparation of the catalyst were investigated. The optimum conditions were: sulfuric acid as coating acid, the concentration of sulfuric 1.1 mol/L, impregnation time 8 h, calcination time 8 h and calcination temperature 450°C. Then hydrolysis of the nut shells was explored, the optimum conditions were as follows: dose of catalyst 20%, ratio of solid to liquid 1:15, reaction temperature 100°C, reaction time 4 h;Under this condition, the yield of furfural was 8.7%.

关键词： Furfural Niobic Acid Nut Shell of Camellia oleifera Abel Hydroliysis

Physicochemical Properties and Nutritional Ingredients of Kernel Oil of <i>Carya cathayensis</i>Sarg

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American Journal of Plant Sciences 2018年第12期9卷 2494-2503页

作者： lisong hu Menghao Du Jingping Zhang Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang China

Carya cathayensis Sarg is distributed near Tianmu mountain areas in Zhejiang Province and Anhui Province, China. Kernel of Carya cathayensis Sarg, which is well-known for the daintiness and nutritional content, is popular and very delicious food in eastern China. In this paper, we investigated the physiochemical properties and nutrient gradients of the kernel oil of Carya cathayensis Sarg. The results showed that the kernel oil of Carya cathayensis Sarg contained 63% of fat, 9.1% of proteins, 3.6% of starch and 4.6% of soluble proteins (based ration of weight). The compositions of the fatty acids were very similar to olive oil, and the total unsaturated fatty acids were 94%. Meanwhile, the kernel oil of Carya cathayensis Sarg also contained some polyphenols such as vanillic acid, ferulic acid, 3-methoxybenzoic acid and quercetin. The content of total polyphenols was 9.7 μg/g. Furthermore, the kernel oil of Carya cathayensis Sarg also contained some trace amount of squalene, β-Sitosterol, Vitamin E.

关键词： Carya cathayensis Sarg Kernel Oil Compositions Fatty Acids

Study on the Composition and Super Critical Carbon Dioxide Extraction of Chinese Hickory (<i>Carya cathayensis</i>Sarg.) Kernel Oil

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American Journal of Analytical Chemistry 2019年第11期10卷 579-589页

作者： lisong hu Menghao Du Shaohai Guo Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang China

Chinese hickory (Carya cathayensis Sarg.) is one of the most productive woody oil-bearing plant in China. Four different extraction methods were explored and supercritical CO2 was selected as green and non-toxic solvent to extract Chinese kernel oil. Four experiment factors, particles size, extraction time, extraction temperature and extraction pressure, were selected to carry out the single factor experiments. According to the results of orthogonal experiments design, the condition of B3C2A2D2 was the optimum reaction parameters. When the experiments were carried out at the optimum parameters, the yield of Chinese hickory kernel oil was 74.5%. The oil fatty acids profiles were analyzed, the results showed that total unsaturated fatty acids were 93.05%. Among them, oleic acid was 66.5 ± 0.44 as the main component. Saturated fatty acids were 6.92 ± 0.21.

关键词： Chinese Hickory Extraction Supercritical CO2 Fatty Acids

Hemicellulose-Based Hydrogels Present Status and Application Prospects: A Brief Review

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Open Journal of Forestry 2018年第1期8卷 15-28页

作者： lisong hu Menghao Du Jinping Zhang Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang China

Hemicellulose is one of the most abundant natural resources, which extensively exist in the pant tissues as the compositions of the cell wall filling between lignin and cellulose. In this review, the hemicellulose based hydrogels was illustrated in details including the hemicellulose derivatives (such as precursor of hemicellulose based hydrogel), hydrogels fabrication methods, origin of hemicellulose, composite hydrogels, and so on. In addition, the authors as well overviewed the new methods for fabrication of hemicellulose based hydrogels and special functional hemicellulose based hydrogels.

关键词： Hemicellulose Hydrogels Graft polymers Swelling Conductivity