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New insights into gibberellin signaling in regulating flowering in Arabidopsis

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Journal of Integrative Plant Biology 2020年第1期62卷 118-131页

作者： shengjie Bao Changmei Hua lisha shen Hao Yu Department of Biological Sciences National University of SingaporeSingapore 117543Singapore Temasek Life Sciences Laboratory National University of SingaporeSingapore 117604Singapore

In angiosperms,floral transition is a key developmental transition from the vegetative to reproductive growth,and requires precise regulation to maximize the reproductive success.A complex regulatory network governs this transition through integrating flowering pathways in response to multiple exogenous and endogenous *** are essential for proper plant developmental regulation and have been extensively studied for their involvement in the floral *** various phytohormones,gibberellin(GA)plays a major role in affecting flowering in the model plant Arabidopsis *** GA pathway interact with other flowering genetic pathways and phytohormone signaling pathways through either DELLA proteins or mediating GA *** this review,we summarize the recent advances in understanding the mechanisms of DELLA-mediated GA pathway in flowering time control in Arabidopsis,and discuss its possible link with other phytohormone pathways during the floral transition.

关键词： Arabidopsis integrating flowering

Resistant starch formation in rice:Genetic regulation and beyond

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Plant Communications 2022年第3期3卷 41-58页

作者： lisha shen Jiayang Li Yunhai Li State Key Laboratory of Plant Cell and Chromosome Engineering CAS Centre for Excellence in Molecular Plant BiologyInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China The Innovative Academy of Seed Design Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of ScienceBeijing 100039China

Resistant starch(RS),a healthy dietary fiber,is a particular type of starch that has attracted much research attention in recent *** has important roles in reducing glycemic index,postprandial blood glucose levels,and serum cholesterol levels,thereby improving and preventing many diseases,such as diabetes,obesity,and cardiovascular *** formation of RS is influenced by intrinsic properties of starch(e.g.,starch granule structure,starch crystal structure,and amylose-to-amylopectin ratio)and non-starch components(e.g.,proteins,lipids,and sugars),aswell as storage and processing *** studies have revealed that several starch-synthesis-related genes(SSRGs)are crucial for the formation of RS during seed *** transcription factors and mRNA splicing factors have been shown to affect the expression or splicing of SSRGs that regulate RS content,suggesting their potential roles in RS *** review focuses mainly on recent research progress on the genetic regulation of RS content and discusses the emerging genetic and molecular mechanisms of RS formation in rice.

关键词： rice resistant starch resistant starch formation genetic regulation starch-synthesis-related

Plant RNA-binding proteins:Phase separation dynamics and functional mechanisms underlying plant development and stress responses

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Molecular Plant 2024年第4期17卷 531-551页

作者： sheng Fan Yu Zhang Shaobo Zhu lisha shen Temasek Life Sciences Laboratory 1 Research LinkNational University of Singapore1 Research LinkSingapore 117604Singapore Department of Biological Sciences National University of Singapore14 Science Drive 4Singapore 117543Singapore

RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in *** RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA *** evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress ***,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA *** also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.

关键词： RNA-binding proteins phase separation biomolecular condensates RNA metabolism development stress resilience

A dephosphorylation-dependentmolecular switch for FT repression mediates flowering in Arabidopsis

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Plant Communications 2024年第3期5卷 167-178页

作者： Yu Zhang Changmei Hua Jian Xuan Kiang lisha shen Temasek Life Sciences Laboratory 1 Research LinkNational University of SingaporeSingapore 117604Singapore Department of Biological Sciences Faculty of ScienceNational University of SingaporeSingapore 117543Singapore

The reproductive success offlowering plants relies greatly on precise timing of thefloral transition,which isnely modulated by a complex network offloral *** a mainfloral integrator,FLOWERING LOCUS T(FT)is also an essential constituent of theflorigen that is transported from leaves to shoot apices to *** is specically transcribed in leaf vascular tissues,where its production is suppressed by manyflowering repressors,including the MYB transcription factor EARLY FLOWERING MYB PROTEIN(EFM).Here,we show that a plant CTD phosphatase,C-TERMINAL DOMAIN PHOSPHATASE-LIKE 2(CPL2),suppresses FT expression in leaf vascular tissues by modulating the binding activity of ***2 interacts with and dephosphorylates EFM to facilitate the binding of dephosphorylated EFM to FT chromatin,thereby *** results suggest that CPL2-mediated dephosphorylation of thefloral repressor EFM serves as a molecular switch,adding another layer of regulation tone-tune FT transcription and ensure thatflowering occurs at an appropriate time.

关键词： CTD phosphatase MYB protein florigen FT flowering Arabidopsis

Nucleoporin 160 Regulates Flowering through Anchoring HOS1 for Destabilizing CO in Arabidopsis

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Plant Communications 2020年第2期1卷 66-76页

作者： Chunying Li Lu Liu Zhi Wei Norman Teo lisha shen Hao Yu Department of Biological Sciences Faculty of ScienceNational University of SingaporeSingapore 117543Singapore Temasek Life Sciences Laboratory National University of SingaporeSingapore 117604Singapore

Nuclear pore complexes(NPCs),which comprise multiple copies of nucleoporins(Nups),are large protein assemblies embedded in the nuclear envelope connecting the nucleus and *** it has been known that Nups affect flowering in Arabidopsis,the underlying mechanisms are poorly ***,we show that loss of function of Nucleoporin 160(Nup160)leads to increased abundance of CONSTANS(CO)protein and the resulting upregulation of FLOWERING LOCUS T(FT)specifically in the *** demonstrate that Nup160 regulates CO protein stability through affecting NPC localization of an E3-ubiquitin ligase,HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1(HOS1),which destabilizes CO protein in the morning *** together,these results provide a mechanistic understanding of Nup function in the transition from vegetative to reproductive growth,suggesting that deposition of HOS1 at NPCs by Nup160 is essential for preventing precocious flowering in response to photoperiod in Arabidopsis.

关键词： nuclear pore complexes HOS1 CO flowering time

Nuclear translocation of OsMADS25 facilitated by OsNAR2.1 in reponse to nitrate signals promotes rice root growth by targeting OsMADS27 and OsARF7

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Plant Communications 2023年第6期4卷 329-341页

作者： Junyu Wu Shuaiqi Yang Nana Chen Qining Jiang Linli Huang Jiaxuan Qi Guohua Xu lisha shen Hao Yu Xiaorong Fan Yinbo Gan Zhejiang Key Laboratory of Crop Germplasm Department of AgronomyCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310000China State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China Temasek Life Sciences Laboratory and Department of Biological Sciences National University of Singapore1 Research LinkSingapore 117604Singapore

Nitrate is an important nitrogen source and signaling molecule that regulates plant growth and *** several components of the nitrate signaling pathway have been identified,the detailed mechanisms are still *** previous results showed that OsMADS25 can regulate root development in response to nitrate signals,but the mechanism is still ***,we try to answer two key questions:how does OsMADS25 move from the cytoplasm to the nucleus,and what are the direct target genes activated by OsMADS25 to regulate root growth after it moves to the nucleus in response to nitrate?Our results demonstrated that OsMADS25 moves from the cytoplasm to the nucleus in the presence of nitrate in an *** immunoprecipitation sequencing,chromatin immunoprecipitation qPCR,yeast one-hybrid,and luciferase experiments showed that OsMADS25 directly activates the expression of OsMADS27 and OsARF7,which are reported to be associated with root ***,OsMADS25-RNAi lines,the Osnar2.1 mutant,and OsMADS25-RNAi Osnar2.1 lines exhibited significantly reduced root growth compared with the wild type in response to nitrate supply,and expression of OsMADS27 and OsARF7 was significantly suppressed in these ***,these results reveal a new mechanismby which OsMADS25 interacts with *** interaction is required for nuclear accumulation of OsMADS25,which promotes OsMADS27 and OsARF7 expression and root growth in a nitratedependent manner.

关键词： OsMADS25 OsNAR2.1 OsMADS27 OsARF7 nitrate signaling rice root

5-Methylcytosine RNA Methylation in Arabidopsis Thaliana

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Molecular Plant 2017年第11期10卷 1387-1399页

作者： Xuean Cui Zhe Liang lisha shen Qian Zhang shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China Department of Biological Sciences and Temasek Life Sciences Laboratory National University of Singapore 117543 Singapore Singapore Institute of Medical Biology Agency for Science Technology and Research (A^+STAR) 8A Biomedical Grove #06-06 Immunos 138648 Singapore Singapore

5-Methylcytosine （m^5C） is a well-characterized DNA modification, and is also predominantly reported in abundant non-coding RNAs in both prokaryotes and eukaryotes. However, the distribution and biological functions of m^5C in plant mRNAs remain largely unknown. Here, we report transcriptome-wide profiling of RNA m^5C in Arabidopsis thaliana by applying m^5C RNA immunoprecipitation followed by a deep- sequencing approach （m^5C-RIP-seq）. LC-MS/MS and dot blot analyses reveal a dynamic pattern of m^5C mRNA modification in various tissues and at different developmental stages, m^5C-RIP-seq analysis identified 6045 m^5C peaks in 4465 expressed genes in young seedlings. We found that m^5C is enriched in coding sequences with two peaks located immediately after start codons and before stop codons, and is associated with mRNAs with low translation activity. We further demonstrated that an RNA （cytosine-5）-methyl- transferase, tRNA-specific methyltransferase 4B （TRM4B）, exhibits m^5C RNA methyltransferase activity. Mutations in TRM4B display defects in root development and decreased m^5C peaks. TRM4B affects the transcript levels of the genes involved in root development, which is positively correlated with their mRNA stability and m^5C levels. Our results suggest that m^5C in mRNA is a new epitranscriptome marker inArabidopsis, and that regulation of this modification is an integral part of gene regulatory networks underlying plant development.

关键词： 5-methylcytosine (m^5C) Arabidopsis RNA methylation TRM4B root development

Regional Brain Metabolic Alterations and Correlation between Clinical Scale Scores and Endocrine Disorders in Anorexia Nervosa Patients

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Regional Brain Metabolic Alterations and Correlation between...

Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence

2014浙江省医学会精神病学分会学术年会、浙江省医师协会精神科医师分会第七届年会

作者： Zheng Lin Yunrong Lu lisha shen Lei Xu Department of Psychiatry The Second Affiliated HospitalMedical School of Zhejiang University Department of Geriatric Diseases The Second Affiliated HospitalMedical School of Zhejiang University

Objectives:Anorexia nervosa(AN) is a psychiatric disorder with a high prevalence and mortality *** brain metabolic changes in AN patients have not been analyzed yet,and study focusing on specific correlation between functional changes in brain and the clinical states is *** study is to investigate these ***:Brian glucose metabolism in 11 AN patients and 11 age- and gender-matched healthy subjects was *** parametric mapping 2(SPM2) was used to compare brain metabolism in the two groups.9 of 11 patients completed the clinical scales.10 of 11 patients were selected to measure hormones in *** significant correlation was found between metabolic changes and clinical ***:Using the SPM2 analysis,higher metabolic changes were found in bilateral frontal lobe,bilateral parietal lobe,left occipital lobe,left temporal lobe,left anterior cingulate,right subcallosal gyrus and right *** broad correlation was found between metabolic changes and clinical indexes,both negatively and ***:This study confirmed that body image disturbance is the core of AN,indicated several brain regions involved in endocrine disorders in AN,and revealed that cerebellum tonsil is also participated in AN.

关键词： anorexia nervosa glucose metabolism clinical scales endocrine disorder

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Molecular Plant 2019年第1期12卷 30-43页

作者： Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub lisha shen Hao Yu Lixi Jiang institute of Crop Science Zhejiang UniversityHangzhou 310058China Temasek Life Sciences Laboratory and Department of Biological Science National University of SingaporeSingapore 117543Singapore Biomarker Technologies Corporation Beijing 101300China Department of Genebank Leibniz institute of Plant Genetics and Crop Plant Research06466 Stadt SeelandGermany Institute of Crop and Nuclear Agricultural Sciences Zhejiang Academy of Agricultural SciencesHangzhou 310021China Office of Research Innovation & CommercializationPMAS-Arid Agricultural University Rawalpindi46300 RawalpindiPakistan Institute of Crop Science Jinhua Academy of Agncultural SciencesJinhua 321017China

Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of "Darmor-bzh"and "Tapidor,"*** generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of *** by calculating the genetic diversity and linkage disequilibrium ***-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding.

关键词： Brassica napus genome resequencing selective sweep ecotype divergence GWAS floweringtime trait