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Single Mutations in the Transmembrane domains of Maize Plasma Membrane Aquaporins Affect the Activity of Monomers within a Heterotetramer

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Molecular Plant 2016年第7期9卷 986-1003页

作者： marie C. Berny dimitri Gilis Marianne Rooman Francois Chaumont Institut des Sciences de la Vie University catholique de Louvain 1348 Louvain-la-Neuve Belgium Bioinformatique genomique et structurale Universite Libre de Bruxelles 1050 Brussels Belgium

Aquaporins are channels facilitating the diffusion of water and/or small uncharged solutes ecross biological membranes. They assemble as homotetramers but some of them also form heterotetramers, especially in plants. In Zea mays, aquaporins belonging to the plasma membrane intrinsic protein （PIP） subfamily are clustered into two groups, PIP1 and PIP2, which exhibit different water-channel activities when expressed in Xenopus oocytes. When PIP1 and PIP2 isoforms are co-expressed, they physically interact to modulate their subcellular localization and channel activity. Here, we demonstrated by affinity chromatography purification that, when co-expressed in Xenopus oocytes, the maize PIP1;2 and PIP2;5 isoforms assemble as homo- and heterodimers within heterotetramers. We built the 3d structure of such heterotetramers by comparative modeling on the basis of the spinach SoPIP2;1 X-ray structure and identified amino acid residues in the transmembrane domains which putatively interact at the interfaces between monomers. Their roles in the water-channel activity, subcellular localization, protein abundance, and physical interac- tion were investigated by mutagenesis. We highlighted single-residue substitutions that either inactivated PIP2;5 or activated PIP1;2 without affecting their interaction. Interestingly, the Phe220Ala mutation in the transmembrane domain 5 of PIP1 ;2 activated its water-channel activity and, at the same time, inactivated PIP2;5 within a heterotetramer. Altogether, these data contribute to a better understanding of the interaction mechanisms between PIP isoforms and the role of heterotetramerization on their water-channel activity.

关键词： aquaporin heterotetramer mutation oligomerization water-channel activity

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Tuning Intermolecular Interaction Between Lignin and Carbon Nanotubes in Fiber Composites–A Combined Experimental and Ab-Initio Modeling Study

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Journal of Renewable Materials 2018年第3期6卷 325-335页

作者： Jan Badorrek Michael Walter marie-Pierre Laborie Freiburger Materialforschungszentrum Stefan-Meier-Straße 21D-79104 Freiburg im BreisgauGermany Chair of Forest Biomaterials Werthmannstraße 6D-79085 Freiburg im BreisgauGermany Freiburg Center for Interactive Materials and Bioinspired Technologies(FIT) Georges-Köhler-Allee 105D-79110 Freiburg im BreisgauGermany Fraunhofer Institute for Mechanics of Materials(IWM) Wöhlerstraße 11D-79108 Freiburg im BreisgauGermany

doping lignin with carbon nanotubes is a promising strategy for cost-effective high-performance carbon *** investigate the intermolecular interaction potential of CNT and organosolv lignin with two main ***,oxidized purified multiwalled carbon nanotubes(MWCNTs)and beech organosolv lignins and derivatives are analyzed with their Hansen solubility parameters(HSPs)to assess their mutual ***,dispersion-corrected density functional theory simulations of the interaction between model molecules and single-walled carbon nanotubes reveal the source of *** find that oxidation enables and enhances the interaction between carbon nanotubes and organosolv lignin experimentally,which is in agreement with the enhanced polar interaction found in the simulations.

关键词： CNT dFT Hansen solubility parameters lignin oxidation

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L’hypnose en oncologie pédiatrique Hypnosis in Pediatric Oncology

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Psycho-Oncologie 2023年第4期17卷 225-233页

作者： Jennifer Marini Audrey Vanhaudenhuyse marie Lacasse Maud Jaspard marie-Elisabeth Faymonville Hadrien Tasset Christophe F.Chantrain Charlotte Grégoire Service d’hémato-oncologie pédiatrique CHC MontLégiaLiège4000Belgique Sensation&Perception Research Group(GIGA Consciousness Universitéde Liège)Liège4000Belgique Centre Interdisciplinaire d’Algologie CHU de LiègeLiège4000Belgique Institut de Cancérologie Arsène Burny CHU de LiègeLiège4000Belgique

Hypnosis is a modified state of consciousness which showed its benefits to decrease different symptoms,mainly *** the last decade,this technique has also been proposed to adults with cancer to improve other symptoms,such as emotional distress,fatigue,or sleep *** pediatric oncology,hypnosis is also used to decrease disease-,treatment-,and procedure-related pain and *** learning of self-hypnosis and self-care techniques also improves the well-being of adults or children with cancer,their parents,and their *** several clinical cases,we will illustrate how hypnosis and self-care techniques have been implemented in the pediatric haemato-oncology department of the CHC MontLégia(Liège,Belgium)and will describe their benefits.

关键词： Pediatric oncology hypnosis analgesia self-monitoring quality of life

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Impact of nanocrystallinity segregation on the growth and morphology of nanocrystal superlattices

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Nano Research 2013年第8期6卷 611-618页

作者： Yanfen Wan Herve Portals Nicolas Goubet Alain Mermet marie-Paule Pileni Université Pierre et marie Curie Paris 6 UMR 7070 LM2N 8P 52 4 place Jussieu 75005 Paris France Centre National de la Recherche Scientifique Unitd Mixte de Recherche 7070 LM2N 4 place Jussieu 75005 Paris France Université Lyon I Institut Lumiére Matiére Unité Mixte de Recherche 5306 CNRS Bāt. A. Kastler 10 rueAda Byron 69622 Villeurbanne France

A colloidal solution of 5 nm Au tetradecanethiol-coated nanoparticles is syn-thesized. After fast evaporation of one drop, ordered monolayers both composed of single domain and polycrystalline nanocrystals are obtained. On increasing the amount of materials and the evaporation time, nanocrystal films with irregular outlines are produced together with close-packed 3d superlattices exhibiting a truncated-tetrahedral shape. Using low-frequency micro-Raman scattering spectroscopy and electron microscopy the building block nanocrystallinity is characterized. Spontaneous nanocrystallinity segregation is revealed： the truncated-tetrahedral supracrystals are shown to mainly contain single domain building blocks while the supracrystalline films are composed of a mixture of single domain and polycrystalline nanocrystals. This observation points out the correlation between the nanocrystallinity segregation involved in the growth of the nanocrystal superlattices and their morphology.

关键词： supracrystals close-packed self-assembly metal nanoparticles Raman scatteringspectroscopy

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Environments favorable to healthy lifestyles:A systematic review of initiatives in Canada

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Journal of Sport and Health Science 2018年第1期7卷 7-18页

作者： Tegwen Gadais Maude Boulanger Francois Trudeau marie-Claude Rivard département des sciences de l’activité physique Universitédu Québec à Montréal département de psychologie Universitédu Québec à Trois Rivières département des sciences de l’activité physique Université du Québec à Trois Rivières

Background: In recent years, a number of initiatives aimed at promoting healthy lifestyles in health-friendly environments have been *** purpose of this review is to synthesize initiatives conducted in Canada and documented in publications for the period 1995–2015 in order to gain a better understanding of their objectives and ***: A systematic review of Canadian initiatives published over the past 20 years was conducted from multiple databases(i.e., Scopus,SPORTdiscus, Pub Med, Academic search complete, ***, Cairn, and Erudit). In total, 264 publications were identified and retained for the final analysis based on 5 criteria:(1) publication between 1995 and 2015,(2) online availability,(3) research conducted in Canada,(4) main topic related to environments favorable to healthy lifestyles(EFHL), and(5) publication in French or ***: A sharp increase in the number of studies on EFHL was observed between 2010 and 2015(57%). Two major lifestyle components—physical activity and nutrition—and 2 environmental aspects—neighborhood and built environment—were the elements most frequently examined regarding adults(48%), young people(34%), and seniors(9%), using quantitative(60%) and qualitative(18%) methods. Furthermore, the analysis reveals a greater focus on the municipal(53%) than the national or provincial levels(31%).Conclusion: This work is a first map of Canadian studies related to EFHL. It clarifies the definition of EFHL and classifies its components. As well,it documents the issues raised, the research methods employed, and the role of stakeholders, while outlining a new research agenda that includes dimensions of EFHL formerly neglected by researchers, namely, political and sociocultural spheres of action.? 2018 Production and hosting by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-Nd license(http://***/licenses/by-nc-nd/4.0/).

关键词： Built environment Canada Favorable environment Healthy lifestyle Initiatives Neighborhood Nutrition Physical activity

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Challenges of Chinese Soft Power Projection Overseas: A Media Narrative Perspective

Challenges of Chinese Soft Power Projection Overseas: A Medi...

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作者： Aisling marie Philippa 上海交通大学

学位级别：硕士

This research project explores how China’s soft power influence,or story,attempts to connect with domestic and overseas *** doing so,it answers the question,“Assess depictions of ‘China’s story’ from articles published by ABC News Australia and People’s daily”.A mixed methods approach was taken to conduct the study,with qualitative case study analysis supported by quantitative content *** two cases selected for the study focused on Australia’s decision to ban China’s leading telecommunications manufacturer,Huawei,from establishing a 5G network within Australia;and the Victorian state government’s decision to sign a Memorandum of Understanding（Mo U）with the Chinese Government and join the Belt and Road Initiative（BRI）.The online segments of two national print publications,China’s the People’s daily and Australia’s Australian Broadcasting Corporation（ABC）,were chosen as primary sources for this *** such,this thesis examines what traditional media shows about China’s soft power influence within *** project was conducted against the backdrop of the era of heightened globalisation where national publications are now accessible internationally,in addition to modern China’s exponential rise,which has simultaneously bolstered China’s economic ties in the Southeast Asia and Pacific regions and heightened the suspicions of China’s more politically liberal ***’s soft power influence has been identified as one tool that it may use to alleviate diplomatic tensions and strengthen its relationships with its geographical *** way that Chinese,and Australian,media publications depict ‘China’s story’ serves as a microcosm for understanding China’s larger quest to accumulate soft power influence internationally.

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Comparative Analysis of Meteorological drought Indices in Quantifying and Characterizing drought Events Over Burundi

Comparative Analysis of Meteorological Drought Indices in Qu...

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作者： Jean marie NdAYIRAGIJE 扬州大学

学位级别：硕士

1.绪论气候变化对与天气有关的自然灾害的发生产生越来越大的影响。本世纪极端天气比以往任何时候都多。气候变化,除了使人口脆弱性和暴露度迅速增加外,还增加了与极端天气相关灾害的风险,包括洪水、干旱、热浪和风暴。此外,自然灾害仍... 详细信息

1.绪论气候变化对与天气有关的自然灾害的发生产生越来越大的影响。本世纪极端天气比以往任何时候都多。气候变化,除了使人口脆弱性和暴露度迅速增加外,还增加了与极端天气相关灾害的风险,包括洪水、干旱、热浪和风暴。此外,自然灾害仍然是政策制定者和管理者的当务之急,尤其是在高风险地区。当这些极端灾难发生的强度高或持续时间长时,可能会对生命安全和社会经济活动造成破坏。在气候变化的情况下,天气变化对水资源的利用和管理产生了巨大影响。世界上有很多人正在遭受水资源短缺危机,或者因为降雨量超过正常平均水平而产生洪涝灾害。干旱是一种自然灾害,由于其对自然环境、生态、水文,特别是农业的有害影响,严重影响人类的生存和经济财产安全。在布隆迪北部,干旱是一种持续时间长且反复发生的自然灾害。尽管该地区容易发生干旱,而且经常遭受干旱,但依然缺乏对该地区旱事件的系统调查与研究。为了量化和表征干旱事件,本研究使用了两个干旱指数,即标准化降水蒸发蒸腾指数(SPEI)和标准化降水指数(SPI),在2个月、6个月和24个月的时间尺度上进行分析。由于布隆迪的大多数种植植物分别需要2至6个月才能完全生长成熟并收获,因此为了与农业生产相对应,采用了以上时间尺度。根据计算的SPEI和SPI值,利用Mann-Kendall(MK)检验和Sen的斜率估计器来分析干旱趋势。本文比较了这两个干旱指数,并定量地阐明SPEI和SPI之间的差异。调查结果显示,1993-2000年和2002-2009年期间,不同类型的干旱威胁着布隆迪北部。这两个指数都表明,2005年、2006年和2007年是极端干旱的年份。SPEI指数检测到的干旱事件分为中度和重度两类,其特征是持续时间长、规模大。相比之下,SPI检测到的干旱事件被归类为“极度干旱”类别,其特征是持续时间有限,烈度较低,但强度较高。这项研究强调,SPEI在量化和表征干旱方面与SPI不同,并建议在评估干旱时同时使用SPEI和SPI。这项研究的结果将有助于布隆迪各地的干旱预防和缓解,特别是有助于农业生产。此外,强烈建议采取积极的缓解干旱的措施、缓解气候变化和完善水资源管理政策,以减少干旱风险及其对人口和自然环境的影响。1.1研究背景干旱事件是发生在全球各地的自然灾害,但其频率、持续时间、严重程度和强度等特征因地形和地理条件而异,因地区和国家而异。根据干旱的严重程度和持续时间,干旱可能对人类和环境产生危险和深远的影响,特别是影响社会、经济和生态系统。气候变化和天气多变等自然现象是造成干旱的主要原因。此外,采矿和砍伐森林等人类活动会加剧干旱的频率和严重程度。干旱事件比较难定义,因为它取决于地区和气候条件、水资源的需求和学科观点的差异。尽管干旱以多种方式影响自然环境和人类生命和财富,但干旱的概念本身是不确定的。直到今天,还没有普遍的干旱定义,但科学家和研究人员就干旱的非常普遍和广为人知的定义达成了一致。一般而言,干旱始于特定地区长时间的降水不足,导致某些农牧业活动、学校和医院等人群或环境部门缺水(***/whatis/***)。另一方面,政府间气候变化专门委员会(Intergovernmental Panel on Climate Change,IPCC)将干旱定义为异常干燥的天气,持续时间长到足以导致严重的水文失衡并导致河流、池塘和湖泊等水体枯竭,世界气象组织(WMO)将干旱定义为持续和长期的降水短缺。1.2研究目的与意义论文的研究目的包括:1.定义不同类型的干旱、其原因和影响;2.干旱事件的量化、表征和分析;3.干旱指数SPEI和SPI的比较分析;4.说明气象干旱指数在干旱监测中的有效性;5.为研究区提出可能的减轻干旱影响的缓解措施。这项研究旨在量化、识别和描述布隆迪的干旱事件。该研究将使人们更好地了解干旱的发生、风险和影响。此外,这项研究的成果将使决策者、规划者和水资源管理者能够在抗旱准备和实施抗旱措施方面做出更多选择。另外,目前很少有关于布隆迪干旱事件的研究。它将作为进一步研究的参考,并提供政策制定和决策制定所需的理论支持,以改善水资源管理和环境保护,特别是减轻干旱。1.3研究内容与技术路线技术路线见图1.1。研究主要包括以下内容:(1)气象数据采集:八个气象站月降水量、最高、最低气温;(2)采用两项气象干旱指数;(3)使用R中的SPEI包生成SPEI和SPI值;(4)干旱量化、干旱事件识别与表征。#12图1-1技术路线图2.研究区域与方法2.1研究区简介布隆迪是一个内陆国家,北部与卢旺达接壤,南部和东部与坦桑尼亚接壤,西部与刚果民主共和国接壤。由于位于赤道带,该国属热带气候。布隆迪的地形以五个不同的微气候区为特征:以洼地和平原为特征的北部和东部,米尔瓦的悬崖,在西部,刚果-尼罗河洋脊将布隆迪的水

关键词：干旱特征 SPEI和SPI指数干旱发生率气候变化 Mann-Kendall检验布隆迪

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Study on the Antecedents of Large-scale Companies' Involvement in Corruption

Study on the Antecedents of Large-scale Companies' Involveme...

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作者： marie-Celine dohle 南京大学

学位级别：硕士

Corruption is dishonest and morally questionable behavior,that probably still exists in every sector and every part of the *** paper discusses corruption in *** aim of this study is to identify antecedents of a large-scale company's engagement in ***,the possible influence of two aspects,home country-level and firm-level characteristics of large-scale companies,is *** country-level characteristics are the corruption level and the cultural values in a company's home ***-level characteristics are the international orientation and the industry of a *** corruption level of a company was calculated by searching and categorizing reported corruption cases in the last ten years of the 500 largest companies in the *** approach distinguishes this study from other studies on corporate corruption,as most of them work with the perceived level of companies' involvement in *** carrying out an OLS regression,the results indicate that the firm-level characteristics international orientation and industry both have an influence on a large-scale company's corruption ***,it was shown that home country characteristics of companies have no such influence.

关键词： Corporate Governance:Bribery/Corruption Logistic Regression Institutional Theory Neo-institutional Theory

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Linking changes in the soil microbial community to C and N dynamics during crop residue decomposition

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Journal of Integrative Agriculture 2021年第11期20卷 3039-3059页

作者： Cyrine REZGUI Isabelle TRINSOUTROT-GATTIN marie BENOIT Karine LAVAL Wassila RIAH-ANGLET AGHYLE Research Unit UniLaSalleRouen-TeamMont-Saint Aignan 76134France Agroecology and Environment Research Unit IsaraAgroSchool for LifeLyon 69007France

Crop residues are among the main inputs that allow the organic carbon(C)and nutrients to be maintained in agricultural *** is an important management strategy that can improve soil fertility and enhance agricultural *** work aims to evaluate the extent of the changes that may occur in the soil heterotrophic microbial communities involved in organic matter decomposition and C and nitrogen(N)mineralization after the addition of crop *** microcosm experiments were performed at 28℃ for 90 days with the addition of three crop residues with contrasting biochemical qualities:pea(Pisum sativum L.),rapeseed(Brassica napus L.),and wheat(Triticum aestivum L.).Enzyme activities,C and N mineralization,and bacterial and fungal biomasses were monitored,along with the bacterial and fungal community composition,by the highthroughput sequencing of 16 S rRNA and ITS *** addition of crop residues caused decreases in β-glucosidase and arylamidase activities and simultaneous enhancement of the C mineralization and net N immobilization,which were linked to changes in the soil microbial *** addition of crop residues decreased the bacterial and fungal biomasses 90 days after treatment and there were shifts in bacterial and fungal diversity at the phyla,order,and genera *** specific orders and genera were dependent on crop residue *** example,Chloroflexales,Inquilinus,Rubricoccus,Clitocybe,and Verticillium were identified in soils with pea residues;whereas Thermoanaerobacterales,Thermacetogenum,and Hypoxylon were enriched in soils with rapeseed residues,and Halanaerobiales,Rubrobacter,and Volutella were only present in soils with wheat *** findings of this study suggest that soil C and N dynamics in the presence of the crop residues were driven by the selection of specific bacterial and fungal decomposers linked to the biochemical qualities of the crop *** crop residue decomposition processes showed specific bacteria

关键词： crop residues C and N mineralization enzyme activities bacterial and fungal diversity

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Interface Characteristics of Silicon Carbide Particles Reinforced Aluminum Composites:Experimental Investigation and Finite Element Simulation

Interface Characteristics of Silicon Carbide Particles Reinf...

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作者： Yves-marie Plard 上海交通大学

学位级别：硕士

随着对金属基复合材料的需求不断增加,以及高力学性能和各向同性的要求,许多研究人员对铝基复合材料进行了实验。本研究建立了模型来了解碳化硅（SiC）颗粒增强铝基复合材料的界面行为。碳化硅颗粒对碳化硅/铝复合材料力学性能的影响取... 详细信息

随着对金属基复合材料的需求不断增加,以及高力学性能和各向同性的要求,许多研究人员对铝基复合材料进行了实验。本研究建立了模型来了解碳化硅（SiC）颗粒增强铝基复合材料的界面行为。碳化硅颗粒对碳化硅/铝复合材料力学性能的影响取决于颗粒的体积分数,分布和大小,同时也取决于显著影响复合材料特性的增强体/基体界面。因此,提取界面特征是至关重要的,它让我们能够预测复合材料的失效机理。本研究的目的是探讨碳化硅颗粒和铝基体之间不同的界面特性。我们将特别关注颗粒形状对复合材料性能的影响。运用纳米压痕实验来获取复合材料的机械性能,并用有限元数值模拟对实验结果进行验证。在构建颗粒/基体界面压痕模型的过程中,涉及到不同复合界面的几何学以及不同铝基体材料的模拟。在实验和模拟部分,分别对4种具有不同屈服强度的基体进行测试:纯Al,Al6061,Al2A14和Al2024。针对每一种复合材料,提取铝和碳化硅界面附近的硬度和模量,结果显示复合材料的硬度和模量对颗粒形状和基体性质具有强烈依赖。最后,数值模型与纳米压痕实验结果的拟实更证明上述结果的精确性。

关键词：金属基复合材料 SiC颗粒纳米压痕界面特征有限元模拟

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