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Influences of the tank liquid lateral sloshing and mass time-varying on high clearance self-propelled sprayer ride comfort

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International Journal of Agricultural and Biological Engineering 2024年第1期17卷 12-22页

作者： liquan Lu Junchang Zhang Cui Cui Jun Chen yu Chen Hongling Jing Shuo Zhang Sugirbay Adilet Chenwei Hu Jiayu Cao College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina Weifang Engineering Vocational College Weifang 261000ShandongChina Technical Faculty S.Seifullin Kazakh Agro Technical UniversityAstana 101400Kazakhstan

To explore the influence of the lateral sloshing and the time-varying mass of the liquid in the tank on the ride comfort of the high-clearance sprayer,a spring-mass-damping equivalent mechanics that can describe the lateral sloshing of the liquid under different filling ratios was constructed based on the equivalent *** Fluent was used to simulate the moment acting on the wall of the tank by the lateral sloshing of the liquid,and then the parameters of the equivalent mechanical model are obtained by fitting and *** analysis of Fluent simulation and bench test on lateral sloshing of tank liquid under different filling *** results show that the lateral sloshing trend of the tank liquid level obtained from the Fluent simulation and the bench test was consistent,which proved the accuracy of the Fluent fluid simulation process and the correctness of the required equivalent mechanical model *** a liquid sloshing equivalent model,a four-degree-of-freedom vertical dynamic model of the sprayer half-car was ***,the performance of the sprayer was systematically analyzed and compared under the excitation of a bump road and a random E-level *** investigation took into account varying liquid filling ratios of 10%,50%,and 90%.The focus lay on evaluating the vertical acceleration of the sprayer body,dynamic deflection of the suspension,and dynamic load on the tires in response to these road *** analysis is conducted independently of the liquid sloshing *** results show that the lateral sloshing of the liquid medicine significantly reduces the ride smoothness of the machine,and makes the vibration response of the machine produce a certain hysteresis *** the reduction of the quality of the liquid medicine in the spray tank,the vibration amplitude of the sprayer body gradually decreases,the hysteresis effect is also gradually *** results presented in this study offer a

关键词： high clearance self-propelled sprayer liquid lateral sloshing ride comfort system dynamics simulation

Als3-Th-cell-epitope plus the novel combined adjuvants of CpG,MDP and FIA synergistically enhanced the immune response of recombinant TRAP derived from Staphylococcus aureus in mice

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Als3-Th-cell-epitope plus the novel combined adjuvants of Cp...

BVDV非结构蛋白NS4B的结构分析及其蛋白表达与鉴定

第十四届全国免疫学学术大会

作者： Jinzhu Ma Wei Liu Simiao yu Baifen Song Yongzhong yu Chunyu Tong liquan yu yudong Cui Heilongjiang Bayi Agricultural University

Introduction:Staphylococcus aureus(***) is a gram-positive opportunistic pathogen,there are currently no high effective vaccine against *** in humans and animals,the development of an efficient vaccine remains an important challenge to prevent *** ***,we prepared Als3-Th-cell-epitope-TRAP(ATT) proteins plus the novel combined adjuvants to develop a promising vaccine candidate against ***:The recombinant pET-28 a(+)-att plasmids were constructed,and the ATT proteins were expressed and obtained,then,ATT plus Freund’s adjuvant or the novel combined adjuvants of CpG,MDP and FIA were immunized in *** booster immunization,the levels of IFN-γ,IL-4,IL-10 and IL-17 A cytokine were evaluated,the humoral immune responses against TRAP were detected in mice,and the survival rate of mice was confirmed by challenge ***:The mice immunized with ATT plus Freund’s adjuvant exhibited significantly higher level of IFN-γ,IL-4,IL-1 0 and IL-17 A,and displayed the stronger humoral immune response against TRAP than control groups,importantly,the survival rate of these mice was significantly higher than control *** addition,compared with the control groups,ATT+CpG+MDP+FIA group was elicited significantly higher level of IFN-γ,IL-4,IL-10 and IL-17 A,and was triggered the stronger humoral immune responses against TRAP,moreover,generated the higher survival rate of ***:Als3 epitopes significantly enhanced TRAP *** plus the novel combined adjuvants of CpG,MDP and FIA induced the strong immune response and protection against ***,revealing the combination of CpG,MDP and FIA adjuvant acts the synergistic effect.

关键词： Als3 epitopes ATT,CpG MDP FIA Combined adjuvants

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黑龙江八一农垦大学学报 2024年第1期36卷 69-76页

作者：柳凯月董亚尊吴双双马一瑄刘鸿雁王北艳于立权宋佰芬马金柱黑龙江八一农垦大学生命科学技术学院大庆163319 中国农业大学动物医学院

NS4B是牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的非结构蛋白,为研究BVDV在病毒侵染宿主细胞过程中的作用,对ns4b进行了结构分析、表达载体构建及蛋白表达。采用Prot-Param、TExpasy和SignalP-4.1等软件分析NS4B蛋白的氨基... 详细信息

NS4B是牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的非结构蛋白,为研究BVDV在病毒侵染宿主细胞过程中的作用,对ns4b进行了结构分析、表达载体构建及蛋白表达。采用Prot-Param、TExpasy和SignalP-4.1等软件分析NS4B蛋白的氨基酸组成、疏水性和空间结构。根据Genebank上公布的BVDVns4b基因序列设计引物,利用PCR方法获得ns4b基因片段,连接到p3×Flag-CMV10表达载体上,构建重组质粒p3×Flag-CMV10-ns4b,经双酶切与测序鉴定正确后,将p3×FlagCMV10-ns4b转染到HEK-293T细胞中,利用Western blot检测NS4B蛋白的表达。结果表明BVDV NS4B蛋白分子量为38.4 kDa,不存在信号肽,有49个磷酸化位点和6个糖基化位点,是一种主要由α-螺旋和无规则卷曲结构组成的疏水性稳定蛋白。成功构建了重组质粒p3×Flag-CMV10-ns4b,并且p3×Flag-CMV10-ns4b在HEK-293T细胞中成功表达,为今后研究NS4B蛋白在BVDV感染宿主细胞及其免疫逃逸机制奠定了基础。

关键词：牛病毒性腹泻病毒 ns4b基因载体构建蛋白表达

Interface and mechanical degradation mechanisms of the silicon anode in sulfide-based solid-state batteries at high temperatures

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Chinese Physics B 2024年第8期33卷 117-126页

作者：王秋辰黄昱力许晶禹习谦李泓陈立泉 Key Laboratory for Renewable Energy Beijing Key Laboratory for New Energy Materials and DevicesBeijing National Laboratory for Condensed Matter PhysicsInstitute of PhysicsChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Huairou Division Institute of PhysicsChinese Academy of SciencesBeijing 101408China

Silicon(Si)is a competitive anode material owing to its high theoretical capacity and low electrochemical ***,the prospect of Si anodes in solid-state batteries(SSBs)has been proposed due to less solid electrolyte interphase(SEI)formation and particle ***,major challenges arise for Si anodes in SSBs at elevated *** this work,the failure mechanisms of Si-Li_(6)PS_(5)Cl(LPSC)composite anodes above 80℃are thoroughly investigated from the perspectives of interface stability and(electro)chemo-mechanical *** chemistry and growth kinetics of Lix Si|LPSC interphase are demonstrated by combining electrochemical,chemical and computational *** and/or Si–P compound formed at Lix Si|LPSC interface prove to be detrimental to interface stability at high *** the other hand,excessive volume expansion and local stress caused by Si lithiation at high temperatures damage the mechanical structure of Si-LPSC composite *** work elucidates the behavior and failure mechanisms of Si-based anodes in SSBs at high temperatures and provides insights into upgrading Si-based anodes for application in SSBs.

关键词： sulfide electrolytes silicon anodes interface stability degradation kinetics all-solid-state batteries

老年癌痛患者数字健康技术焦虑现状及影响因素

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中国实用护理杂志 2023年第28期39卷 2224-2229页

作者：李利潘树波余立权安徽医科大学第二附属医院普外科合肥230601

目的调查老年癌痛患者使用数字健康技术中技术焦虑情况,并分析其影响因素,为实施技术焦虑的相关干预和个性化数字健康服务提供有价值的参考。方法采用便利抽样法选取2018年3月至2022年10月安徽医科大学第二附属医院普外科接受治疗的老... 详细信息

目的调查老年癌痛患者使用数字健康技术中技术焦虑情况,并分析其影响因素,为实施技术焦虑的相关干预和个性化数字健康服务提供有价值的参考。方法采用便利抽样法选取2018年3月至2022年10月安徽医科大学第二附属医院普外科接受治疗的老年癌痛患者160例为研究对象。采用一般资料调查表、技术焦虑量表、家庭亲密度与适应量表和老年人社交网络量表对患者进行横断面调查。采用多元线性回归法分析老年癌痛患者院外移动医疗技术焦虑得分的影响因素。结果最终有效回收问卷153份。153例老年癌痛患者技术焦虑量表总分为(45.24±9.67)分,多元线性回归分析显示,家庭人均月收入(t=-2.89,P=0.004)、居住情况(t=-2.04,P=0.043)、家庭支持(t=-2.42,P=0.017)和社交网络(t=-7.81,P<0.001)是老年癌痛患者技术焦虑得分的主要影响因素。结论家庭收入较低、独居、家庭支持得分较低、社交网络得分较低的老年癌痛患者在出院后数字健康技术焦虑得分较高,对于此类患者应提供个体化干预措施,以降低其数字健康技术焦虑水平。

关键词：老年人癌痛数字健康技术焦虑影响因素

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新型海底管道连接器密封性能的优化

华中科技大学学报（自然科学版） 2017年第3期45卷 40-45页

作者：魏宗亮王立权关雨李绍凯哈尔滨工程大学机电工程学院黑龙江哈尔滨150001

为了改善新型海底管道连接器的密封性能,给出了一种连接器关键结构参数的优化方法.论述了连接器的结构形式和工作原理,建立了以连接器接触压力方差最小化和最大塑性应变最小化为目标的多目标优化模型,运用径向基函数法建立了目标函数、... 详细信息

为了改善新型海底管道连接器的密封性能,给出了一种连接器关键结构参数的优化方法.论述了连接器的结构形式和工作原理,建立了以连接器接触压力方差最小化和最大塑性应变最小化为目标的多目标优化模型,运用径向基函数法建立了目标函数、约束变量与设计变量之间的数学模型,给出了连接器关键设计变量的确定方法,推导了临界接触压力均值的表达式.以某连接器为例,计算得到临界接触压力均值为370MPa,给出了影响密封性能的重要结构参数偏距与过盈量的优化方法,优化结果与有限元结果基本相同.最后对优化前后的连接器进行了对比实验,结果表明:优化后的连接器具有较高的承受内压与外载荷的能力,连接器的密封性能得到明显提高.

关键词：连接器密封接触压力均值接触压力方差径向基函数多目标优化

酿酒酵母丝氨酸/苏氨酸蛋白磷酸酯酶的功能与调控

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生物技术通报 2009年第12期25卷 17-21页

作者：蒋伶活于立权天津医科大学天津市基础医学研究中心天津300070 天津大学药物科学与技术学院天津300072

从酿酒酵母蛋白磷酸酯酶的分类和结构特征入手,阐述了该蛋白家族中的亚家族成员丝氨酸/苏氨酸蛋白磷酸酯酶的功能和表达调控。深入研究酿酒酵母丝氨酸/苏氨酸蛋白磷酸酯酶,特别是PP2C蛋白磷酸酯酶的细胞功能及其调控,将对新药研发和疾... 详细信息

从酿酒酵母蛋白磷酸酯酶的分类和结构特征入手,阐述了该蛋白家族中的亚家族成员丝氨酸/苏氨酸蛋白磷酸酯酶的功能和表达调控。深入研究酿酒酵母丝氨酸/苏氨酸蛋白磷酸酯酶,特别是PP2C蛋白磷酸酯酶的细胞功能及其调控,将对新药研发和疾病干预治疗提供重要基础。

关键词：酿酒酵母丝氨酸/苏氨酸蛋白磷酸酯酶调控机制

Identification of a conserved linear B-cell epitope of Streptococcus GapC protein by phage-displayed peptide library

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Identification of a conserved linear B-cell epitope of Strep...

动物生态养殖、疫病防控、食品安全与人类健康——中国畜牧兽医学会家畜传染病学分会第八届全国会员代表大会暨第十五次学术研讨会

作者： Limeng Zhang Xue Zhou Ziyao Fan Wei Tang Hunan Sun liquan yu Zhanbo Zhu Fanze Piao yudong Cui College of Life Science and Technology Heilongjiang Bayi Agricultural University

Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis are three common pathogens causing bovine mastitis. The GapC of Streptococcus is a highly conserved surface layer protein that is responsible for inducing protective immune response in mice. To identify protective epitope of GapC, a monoclonal antibody (mAb1F2) against the recombinant GapC of S. dysgalactiae was generated by screening a phage-displayed random 12-peptide library. After three rounds of biopanning, 25 phage clones were randomly selected and their specificity to the mAb was verified by sandwich ELISA. Their amino acid of 13 among total 25 phage colones were sequenced and deduced. It was found that their amino aicd sequences can match amino acids 30TRINDLT36 of GapC. It showed that mAb1F2 could react with the motif 30TRINDLT36 fused GST in Western bloting. So it proposed that the motif 30TRINDLT36 represented a linear B-cell epitope of the GapC. A single replacement of amino acid for the epitope 30TRINDLT36 demonstrated that residues R31A, I32A, N33A, D34A and L35A constitute the core of the epitope. The high homology was found after alignment of the epitope sequences from different streptococcus species. The identified epitope would be useful in development of epitope-based marker vaccines against Streptococcus infection.

关键词： S. dysgalactiae GapC monoclonal antibody epitope mapping phage display

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公安应用写作课堂教学模式研究

吉林公安高等专科学校学报 2003年第6期18卷 63-65页

作者：于力泉吉林公安高等专科学校吉林长春130117

从公安应用写作课程的特点出发,构建符合公安工作实际需要的课堂教学模式和教学方法,推行教育创新,提高教学质量,是一个在教学实践和理论研究中亟待解决的问题。构建科学的公安应用写作课堂教学模式,就要不断优化教学结构,提高课堂效益... 详细信息

从公安应用写作课程的特点出发,构建符合公安工作实际需要的课堂教学模式和教学方法,推行教育创新,提高教学质量,是一个在教学实践和理论研究中亟待解决的问题。构建科学的公安应用写作课堂教学模式,就要不断优化教学结构,提高课堂效益;不断充实更新教学内容,挖掘创新因素;并应注重从学生入手,开发他们的潜能,进一步完善课程建设。

关键词：公安应用写作课堂教学模式构建