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OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice

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Journal of Genetics and Genomics 2022年第8期49卷 766-775页

作者： Meiru Jia Nan Luo Xiangbing Meng Xiaoguang Song Yanhui Jing liquan kou Guifu Liu Xiahe Huang Yingchun Wang Jiayang Li Bing Wang Hong Yu State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China

Salt stress adversely affects plant growth,development,and crop yield.Rice(Oryza sativa L.)is one of the most salt-sensitive cereal crops,especially at the early seedling stage.Mitogen-activated protein kinase(MAPK/MPK)cascades have been shown to play critical roles in salt response in Arabidopsis.However,the roles of the MPK cascade signaling in rice salt response and substrates of Os MPK remain largely unknown.Here,we report that the salt-induced Os MPK4-Ideal Plant Architecture 1(IPA1)signaling pathway regulates the salt tolerance in rice.Under salt stress,Os MPK4 could interact with IPA1 and phosphorylate IPA1 at Thr180,leading to degradation of IPA1.Genetic evidence shows that IPA1 is a negative regulator of salt tolerance in rice,whereas Os MPK4 promotes salt response in an IPA1-dependent manner.Taken together,our results uncover an Os MPK4-IPA1 signal cascade that modulates the salt stress response in rice and sheds new light on the breeding of salt-tolerant rice varieties.

关键词： Oryza sativa MPK OsMPK4 IPA1 Salt tolerance

SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana

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Science China(Life Sciences) 2023年第1期66卷 2-11页

作者： Xilong Li Zongyun Yan Meiling Zhang Jiayin Wang Peiyong Xin Shujing Cheng liquan kou Xiaoting Zhang Songlin Wu Jinfang Chu Chengqi Yi Keqiong Ye Bing Wang Jiayang Li State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China Key Laboratory of RNA Biology CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100039China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

Polyamines have been discovered for hundreds of years and once considered as a class of phytohormones.Polyamines play critical roles in a range of developmental processes.However,the molecular mechanisms of polyamine signaling pathways remain poorly understood.Here,we measured the contents of main types of polyamines,and found that endogenous level of thermospermine(T-Spm)in Arabidopsis thaliana is comparable to those of classic phytohormones and is significantly lower than those of putrescine(Put),spermidine(Spd),and spermine(Spm).We further found a nodule-like structure around the junction area connecting the shoot and root of the T-Spm biosynthetic mutant acl5 and obtained more than 50 suppressors of acl5 nodule structure(san)through suppressor screening.An in-depth study of two san suppressors revealed that NAP57 and NOP56,core components of box H/ACA and C/D snoRNPs,were essential for T-Spm-mediated nodule-like structure formation and plant height.Furthermore,analyses of rRNA modifications showed that the overall levels of pseudouridylation and 2′-O-methylation were compromised in san1 and san2 respectively.Taken together,these results establish a strong genetic relationship between rRNA modification and T-Spm-mediated growth and development,which was previously undiscovered in all organisms.

关键词： polyamines thermospermine snoRNP pseudouridylation 2’-O-methylation Arabidopsis thaliana

Extensive sequence divergence between the reference genomes of Taraxacum kok-saghyz and Taraxacum mongolicum

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Science China(Life Sciences) 2022年第3期65卷 515-528页

作者： Tao Lin Xia Xu Huilong Du Xiuli Fan Qingwen Chen Chunyan Hai Zijian Zhou Xiao Su liquan kou Qiang Gao Lingwei Deng Jinsheng Jiang Hanli You Yihua Ma Zhukuan Cheng Guodong Wang Chengzhi Liang Guomin Zhang Hong Yu Jiayang Li State Key Laboratory of Plant Genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Agrobiotechnology College of HorticultureChina Agricultural UniversityBeijing 100193China University of Chinese Academy of Sciences Beijing 100049China Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Biotechnology Research Institute Heilongjiang Academy of Agricultural SciencesHarbin 150086China Guangdong Laboratory of Lingnan Modern Agriculture Guangzhou 510642China School of Life Sciences Institute of Life Sciences and Green DevelopmentHebei UniversityBaoding 071002China

Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs more investigation.Here,we presented high-quality genome assemblies of rubber-producing T.kok-saghyz TK1151 and non-rubber-producing T.mongolicum TM5.Comparative analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two species.Two gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene amplification.In genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to TM5.This study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.

关键词： reference genome Taraxacum kok-saghyz Taraxacum mongolicum comparative genomics natural rubber biosynthesis pathway

Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice

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Molecular Plant 2023年第11期16卷 1811-1831页

作者： Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang liquan kou Xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Life Sciences Shandong Agriculture UniversityTai’anShandong 271018China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China Yazhouwan National Laboratory SanyaHainan 572024China

Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(Pi)starvation.However,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of Pi and nitrogen remain largely unclear.Here,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental responses.We found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-Pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in rice.Interestingly,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under Pi deficiency.We also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote Pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in rice.Importantly,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus conditions.Taken together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to Pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-Pi environments.

关键词： rice Pi deficiency strigolactone NSP1 and NSP2 CRL1 nitrogen and Pi absorption

硫代磷酸酯类杀虫剂ScFv抗体库的构建与初步筛选

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西北农业学报 2014年第4期23卷 202-206页

作者：寇立泉刘爽王东方祁志军西北农林科技大学农药研究所陕西杨凌712100

旨在构建硫代磷酸酯类杀虫剂单链抗体(ScFv)库,并对其进行初步筛选。采用RT-PCR法从牛血清蛋白(BSA)人工抗原免疫的Balb/C小鼠脾细胞中克隆出VH和VL基因,通过重叠延伸PCR方法将VH和VL基因拼接在一起,构建ScFv基因,与表达载体pCANTAB 5E... 详细信息

旨在构建硫代磷酸酯类杀虫剂单链抗体(ScFv)库,并对其进行初步筛选。采用RT-PCR法从牛血清蛋白(BSA)人工抗原免疫的Balb/C小鼠脾细胞中克隆出VH和VL基因,通过重叠延伸PCR方法将VH和VL基因拼接在一起,构建ScFv基因,与表达载体pCANTAB 5E连接后,在大肠杆菌中诱导表达。采用亲和富集法,经6轮淘筛后用酶联免疫吸附分析(ELISA)测定其活性。得到滴度为2.2×106 pfu/mL噬菌体库,ELISA检测呈阳性。说明成功表达并筛选得到鼠源抗硫代磷酸酯类杀虫剂ScFv抗体库。

关键词：硫代磷酸酯类杀虫剂单链抗体库 ELISA