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Liquid chromatography–tandem mass spectrometry method for simultaneous determination of valproic acid and its ene-metabolites in epilepsy patient plasma

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Journal of Pharmaceutical Analysis 2016年第2期6卷 112-116页

作者： Huan Lu Chong Su Lei Yin Liqiang Gu Jingkai gu Xiaohui Chen School of Pharmacy Shenyang Pharmaceutical University Research Center for Drug Metabolism College of Life ScienceJilin University Clinical Pharmacology Center Research Institute of Translational Medicine the First Hospital of Jilin University

A simple and high throughput method was developed and validated for simultaneous determination of valproic acid and its two toxicant ene-metabolites, 2-enevalproic acid and 4-enevalproic acid in epilepsy patient plasma using liquid chromatography-tandem mass spectrometry. Probenecid was used as in- ternal standard and solid-phase extraction was selected for sample preparation. A chromatographic separation was performed on an Agilent Poroshell SB-C18 column （50 mm × 4.6 mm i.d., 2.7μm） by an optimized gradient elution at a flow rate of 0.9 mL/min. The total run time was 7 rain. Electrospray ionization was used in negative ion mode by multiple reaction monitoring of the precursor-to-product ion transitions at m/z 143.0→143.0 for valproic acid, m/z 140.9 →140.9 for 2-enevalproic acid and 4-enevalproic acid for their poor fragments, and m/z 283.9→239,9 for probenecid. The results showed good linearity ofvalproic acid, 2-enevalproic acid and 4-enevalproic acid in their respective linear ranges. The correlation coefficients were more than 0.998, The intra- and inter-day precision of the assay was less than 11.0% and the accuracy ranged from 2% to 12%. This analytical method was successfully applied to assay plasma concentrations of valproic acid and its two ene-metabolites in epilepsy patient plasma and used for therapeutic drug monitoring.

关键词： Liquid chromatography-tandem massspectrometry Valproic acid 2-enevalproic acid 4-enevalproic acid

从原位缝合到显微血管吻合——断指再植历史考证

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中华显微外科杂志 2023年第5期46卷 481-486页

作者：顾立强《中华显微外科杂志》编辑部广州510062 中山大学附属第一医院显微创伤手外科广州510080

1959年,Douglas报道完全离断指尖原位缝合-复合移植成活;1960年代初,Jacobson、Buncke分别行口径1.4 mm、0.8 mm实验性显微血管缝合及猴断指再植成功;1962年,Kleinert不完全离断拇指显微血管缝合再植成功;1965年,Tamai完全离断拇指显微... 详细信息

1959年,Douglas报道完全离断指尖原位缝合-复合移植成活;1960年代初,Jacobson、Buncke分别行口径1.4 mm、0.8 mm实验性显微血管缝合及猴断指再植成功;1962年,Kleinert不完全离断拇指显微血管缝合再植成功;1965年,Tamai完全离断拇指显微再植成功;1967年,陈中伟首次英文报道断指再植成功20例;1972年10月,O'Brien访华并报道中国断(指)肢再植技术、经验;1972年11月召开的广州断肢再植经验交流会上,总结完全断指260例398指,再植成功179指(占44.9%);1973年5月,北美断肢再植医师代表团访华并发布访华报道,中国断肢(指)再植技术与原则被国际同行认可。1974年1月,陈中伟率中国显微外科代表团访美,被邀作断肢(指)再植创始者报道,进一步扩大了中国断肢(指)再植的国际影响。

关键词：断指再植原位缝合显微血管吻合显微外科学科史

Effect of DOPO-containing Flame Retardants on Poly（lactic acid）： Non-flammability, Mechanical Properties and Thermal Behaviors

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Chemical Research in Chinese Universities 2017年第1期33卷 143-149页

作者： gu liqiang QIU Jianhui SAKAI Eiichi Department of Machine Intelligence and Systems Engineering Faculty of Systems Science and Technology Akita Prefectural University Yurihonjo 015-0055 Japan

The flame retardancies of three kinds of 9,10-dihydro-9-oxa-10-phosphaphenan-threne 10-oxide（DOPO）- containing flame retardant（A1, A2, A3）/poly（lactic acid）（PLA） composites[PA-n/（Ax-y）, n= 1--12; x= l, 2, 3, denoting three kinds of flame retardants; y= 10%, 20%, 30%, 40%, denoting the mass fraction of Ax] were greatly enhanced by melt blending of flame retardant Ax with PLA, including twin-screw extrusion and injection-molding processes. With only 10%（mass fraction） of Ax added to PLA, good flame retardancy with limiting oxygen index（LOI） values of more than 33% was achieved. As the Ax mass fraction was further increased to 20%, PA-n/（Ax-20%） composites showed much better flame retardancy（LOI~〉35% and UL-94 V-0 rating）. Moreover, the thermal degradation behaviors and mechanical properties of PA-n/（Ax-y） composites were investigated via thermogravimetric analysis（TGA）, differen- tial thermal analysis（DTA）, tensile testing, notched impact-bar testing, and dynamic mechanical analysis（DMA）. TGA results show that PA-n/（Ax-y） composites have slower rate of mass loss and much higher char yield, compared to neat PLA. With the addition of Ax to PLA, the DTA and DMA results indicate slight variations in glass transition tcmpe- ratures（Tg） of PA-n/（Ax-y） composites. Based on TGA results under nonisothermal conditions, the thermal degrada- tion kinetics of PA-n/（Ax-y） composites were studied by KAssinger＇s and Ozawa＇s methods. These thermal degrada- tion dynamic analyses show lower activation energies（EK or Eo） for PA-n/（Ax-y） composites, corresponding to higher mass fractions of Ax（from 10% to 40%）. The PA-n/（Ax-y） composites with good flame retardancy and good mecha- nical properties obtained in this study could be potential candidates for fire- and heat-resistant applications in auto- motive engineering and building fields with more safety and excellent performance.

关键词： Poly（lactic acid） composite Flame retardancy Thermal degradation kinetics

Biological fate and interaction with cytochromes P450 of the nanocarrier material,D-α-tocopheryl polyethylene glycol 1000 succinate

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Acta Pharmaceutica Sinica B 2022年第7期12卷 3156-3166页

作者： Tianming Ren Runzhi Li liqiang Zhao J.Paul Fawcett Dong Sun Jingkai gu Research Center for Drug Metabolism School of Life SciencesJilin UniversityChangchun 130012China State Key Laboratory of Supramolecular Structure and Materials Jilin UniversityChangchun 130012China Beijing Institute of Drug Metabolism Beijing 102209China

D-a-Tocopheryl polyethylene glycol 1000 succinate(TPGS,also known as vitamin E-TPGS)is a biodegradable amphiphilic polymer prepared by esterification of vitamin E with polyethylene glycol(PEG)1000.It is approved by the US Food and Drug Administration(FDA)and has found wide application in nanocarrier drug delivery systems(NDDS).Fully characterizing the in vivo fate and pharmacokinetic behavior of TPGS is important to promote the further development of TPGS-based NDDS.However,to date,a bioassay for the simultaneous quantitation of TPGS and its metabolite,PEG1000,has not been reported.In the present study,we developed such an innovative bioassay and used it to investigate the pharmacokinetics,tissue distribution and excretion of TPGS and PEG1000 in rat after oral and intravenous dosing.In addition,we evaluated the interaction of TPGS with cytochromes P450(CYP450s)in human liver microsomes.The results show that TPGS is poorly absorbed after oral administration with very low bioavailability and that,after intravenous administration,TPGS and PEG1000 are mainly distributed to the spleen,liver,lung and kidney before both being slowly eliminated in urine and feces as PEG1000.In vitro studies show the inhibition of human CYP450 enzymes by TPGS is limited to a weak inhibition of CYP3A4.Overall,our results provide a clear picture of the in vivo fate of TPGS which will be useful in evaluating the safety of TPGS-based NDDS in clinical use and in promoting their further development.

关键词： TPGS LC-MS/MS Nanocarrier materials Pharmacokinetics Tissue distribution Metabolism Excretion Cytochrome P450

断肢再植成功与显微外科发展——纪念陈中伟中国首例断肢再植成功60周年

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中华显微外科杂志 2023年第6期46卷 601-604页

作者：顾立强《中华显微外科杂志》编辑部广州510062 中山大学附属第一医院显微创伤手外科广州510080

1963年1月2日,上海市第六人民医院陈中伟、鲍约瑟和钱允庆用血管套接法成功实施世界首例完全离断前臂断肢再植,随访7个月获得良好功能,患者已能用伤肢举杯饮水、持重6 kg、写字和打乒乓球。1963年9月在罗马召开的第20届国际外科会议上... 详细信息

1963年1月2日,上海市第六人民医院陈中伟、鲍约瑟和钱允庆用血管套接法成功实施世界首例完全离断前臂断肢再植,随访7个月获得良好功能,患者已能用伤肢举杯饮水、持重6 kg、写字和打乒乓球。1963年9月在罗马召开的第20届国际外科会议上首次报告(崔之义代表发言),1963年10月中华医学会主办的Chinese Medical Journal等3本杂志同时中、英文报道了陈中伟团队的创新工作,这是国际科技期刊首次报道人类完全性离断肢体再植手术成功(图1)[1]。

关键词：断肢再植中华医学会陈中伟打乒乓球显微外科钱允庆完全离断

Electrochemical detection of methyl-paraoxon based on bifunctional cerium oxide nanozyme with catalytic activity and signal amplification effect

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Journal of Pharmaceutical Analysis 2021年第5期11卷 653-660页

作者： Yuzhou Sun Jinchao Wei Jian Zou Zehua Cheng Zhongming Huang Liqiang Gu Zhangfeng Zhong Shengliang Li Yitao Wang Peng Li State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao999078China Institute of Traditional Chinese Medicine&Natural Products College of PharmacyJinan UniversityGuangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs ResearchGuangzhou510632China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou510632China Center of Super-Diamond and Advanced Films(COSDAF)and Department of Chemistry City University of Hong KongHong Kong999077China

A new electrochemical sensor for organophosphate pesticide(methyl-paraoxon)detection based on bifunctional cerium oxide(CeO_(2))nanozyme is here reported for the first time.Methyl-paraoxon was degraded into p-nitrophenol by using CeO_(2) with phosphatase mimicking activity.The CeO_(2) nanozymemodified electrode was then synthesized to detect p-nitrophenol.Cyclic voltammetry was applied to investigate the electrochemical behavior of the modified electrode,which indicates that the signal enhancement effect may attribute to the coating of CeO_(2) nanozyme.The current research also studied and discussed the main parameters affecting the analytical signal,including accumulation potential,accumulation time,and pH.Under the optimum conditions,the present method provided a wider linear range from 0.1 to 100 mmol/L for methyl-paraoxon with a detection limit of 0.06 mmol/L.To validate the proof of concept,the electrochemical sensor was then successfully applied for the determination of methyl-paraoxon in three herb samples,i.e.,Coix lacryma-jobi,Adenophora stricta and Semen nelumbinis.Our findings may provide new insights into the application of bifunctional nanozyme in electrochemical detection of organophosphorus pesticide.

关键词： Chinese medicine Nanozyme Organophosphorus Pesticide Methyl-paraoxon Electroanalysis

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周围神经学组建设与学术进展

中国修复重建外科杂志 2018年第7期32卷 786-791页

作者：顾立强中山大学附属第一医院骨科-显微外科医学部显微创伤手外科广州510080

中国康复医学会修复重建外科专业委员会周围神经学组成立于1995年,在老一辈专家带领下,周围神经损伤修复、再生、重建取得了重大研究进展。1986年顾玉东教授首创了健侧C_7神经移位术治疗臂丛根性撕脱伤,目前该术式临床应用已三十余年,... 详细信息

中国康复医学会修复重建外科专业委员会周围神经学组成立于1995年,在老一辈专家带领下,周围神经损伤修复、再生、重建取得了重大研究进展。1986年顾玉东教授首创了健侧C_7神经移位术治疗臂丛根性撕脱伤,目前该术式临床应用已三十余年,安全、有效,近十年来也获得了改良、发展。除此以外,健侧C_7移位治疗上肢痉挛瘫、健侧C_7椎体前移位修复全臂丛根性撕脱伤、脊髓损伤后膀胱功能重建、去细胞同种异体神经修复材料的研制与开发、小间隙套接缝合技术、股薄肌功能性移植重建、健侧S_1神经根移位术,均已在临床推广应用,并取得良好疗效。随着周围神经修复材料的生物制造、臂丛损伤后中枢神经系统的重塑规律研究取得进展,神经科学的研究范围也得到进一步拓展。坚持周围神经显微修复、重建技术的普及与提高,努力使周围神经研究成果向临床转化,提高我国周围神经外科的学术水平,仍是周围神经外科医生与学者的艰巨任务。

关键词：周围神经神经损伤神经修复

Lactoferrin improves hepatic pyroptosis in mice after irradiation

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Radiation Medicine and Protection 2022年第1期3卷 16-21页

作者： Ru Zhang Jia gu Yulu Wei Yaxing guo liqiang Qin Jiaying Xu Department of Nutrition and Food Hygiene School of Public HealthSoochow UniversitySuzhou 215123China Suzhou National High-tech District Center for Disease Prevention and Control Suzhou 215011China State Key Laboratory of Radiation Medicine and Protection Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsSchool of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China

Objective:To investigate the effects of lactoferrin(Lf)on hepatic pyroptosis(an inflammatory form of programmed cell death)in mice exposed to radiation.Methods:A total of thirty-six BALB/c male mice were randomly divided into four groups,namely the control group,5 Gy group,5 Gyþ2 mg Lf group,and 5 Gyþ4 mg Lf group.The mice were administered whole-body ionizing radiation using a PRIMUS accelerator,with a single dose of 5 Gy and an absorbed dose rate of 2.0 Gy/min at a source-skin distance of 100 cm.Lf solution was intraperitoneally injected into the mice 2 h before and per day after radiation.The mice were sacrificed 1,3,and 9 d after radiation,and their livers were used for histopathologic examination,immunohistochemistry analysis,and Western blot analysis for absent in melanoma 2(AIM2)inflammasome pathway.Results:Histopathologic examination showed the disorder of the hepatocellular structure and the accumulation of inflammatory cells after radiation.Lf intervention inhibited hepatocellular proliferation and decreased the infiltration of inflammatory cells.Immunohistochemistry analysis indicated that AIM2 overexpression was significantly attenuated by 4 mg of Lf(t=3.065,Pgulated the hepatic overexpression of AIM2,apoptosis-associated speck-like protein containing a caspase activation and recruitment domain(ASC),IL-1β,and IL-18.2 mg of Lf significantly downregulated the abovementioned protein expression 3 d(t=7.934,4.092,5.193,2.916,Pgulating the protein expression of AIM2 inflammasome.

关键词： Lactoferrin Irradiation Liver Pyroptosis Absent in melanoma 2(AIM2)

中国显微外科史考证:艰难起步(1960-1973)

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中华显微外科杂志 2021年第5期44卷 584-590页

作者：顾立强《中华显微外科杂志》编辑部广州510065 中山大学附属第一医院显微创伤手外科广州510080

目的以文献为准、以时间为序,梳理中国显微外科1960-1973年这个阶段的历史。方法检索收集公开及内部出版中、英文科技期刊、学术专著等,精读分析中、英文"显微外科"及"显微外科相关"文献,重点记录确认手术、交流、报道时间等。结果中国... 详细信息

目的以文献为准、以时间为序,梳理中国显微外科1960-1973年这个阶段的历史。方法检索收集公开及内部出版中、英文科技期刊、学术专著等,精读分析中、英文"显微外科"及"显微外科相关"文献,重点记录确认手术、交流、报道时间等。结果中国显微外科起步,得益于1963年初上海市第六人民医院世界首例断肢再植成功报道;此后3年,多个单位开展显微血管技术实验研究、探索断指再植,于1964年世界首例断腿再植,于1966年世界首例足趾移植再造拇指、世界首批完全离断手指显微血管缝合再植成功;1972年广州全国断肢再植经验交流会,完全断指260例,再植存活率44.9%;首次使用"显微外科"术语;1973年3月,完成世界第2例腹股沟皮瓣游离移植;1973年5月,北美再植代表团访华,开启中、外显微外科学术交流先河。近十年临床实践、原创性、先进的中国断肢(指)再植技术与原则被国际同行认可。结论中国显微外科史考证:艰难起步(1960-1973)——中国显微外科发展基本与世界同步。

关键词：中国显微外科断肢再植断指再植足趾移植拇指再造学科史