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Molecular Detection of Extended-Spectrum Beta Lactamase and Carbapenemase Genes in Escherichia coli and Salmonella spp. Isolated from Chickens Consumed Outdoors in Ouagadougou, Burkina Faso

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Journal of Biosciences and Medicines 2024年第11期12卷 221-232页

作者： Amana Mètuor Dabiré Stéphanie Lynseh Carine Sita Bénao Nicolas Ouédraogo Rahimatou Yasmine Wendkouni Tiemtoré Rhaina Olivia Badini lionel eliada benoit bambara Jacques Simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center Department of Biochemistry-Microbiology Joseph Ki-Zerbo University Ouagadougou Burkina Faso Department of Biochemistry-Microbiology University of Ddougou Ddougou Burkina Faso Centre National de Recherche et de Formation sur le Paludisme Institut National de Sant Publique Ouagadougou Burkina Faso Laboratoire de biochimie et immunologie applique (LABIA) Joseph Ki-Zerbo University Ouagadougou Burkina Faso

This study was carried out to assess the prevalence of resistance genes in strains of Escherichia coli and Salmonella spp. isolated from free-range chickens in Ouagadougou, where resistant bacteria can be transmitted to humans via faeces or contaminated meat. A total of 280 strains of Escherichia coli and 129 strains of Salmonella spp. resistant to at least one beta-lactam or carbapenem antibiotic were used in this study. PCR analyses revealed the presence of ESBL (extended spectrum beta lactamase) resistance genes in Escherichia coli isolates, with 3.21% (9/280) possessing the CTX-M (Cefotaximase) gene, 15.35% (43/280) had the SHV (Sulfhydril Variable) gene, and 11.42% (32/280) had carbapenemase resistance genes, more specifically IMP (Imipenemase metallo-beta-lactamase). As regards Salmonella spp. strains, only the presence of the SHV (Sulfhydril Variable) gene was identified in 2.32% (3/129) of isolates belonging to the ESBL family, while 26.35% (34/129) and 13.95% (18/129) of isolates respectively possessed the IMP (Imipenemase metallo-beta-lactamase) and NDM (New Delhi metallo-β-lactamase) genes, both of the carbapenemase type. The significant prevalence of resistance genes in bacterial strains isolated from chickens sold outdoors in Ouagadougou raises major public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Bacteria Resistance Extended-Spectrum Beta Lactamases Carbapenemases Ouagadougou

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Multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 881-892页

作者： Stéphanie Lynseh Carine Sita Bénao Dabiré Amana Métuor Abdoul Karim Ouattara Rahimatou Yasmine Wendkouni Tiemtoré Nicolas Ouédraogo Blandine Ouédraogo Rhaina Olivia Badini lionel eliada benoit bambara Serge Sougué Jacques Simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center (CERBA) Department of Biochemistry-Microbiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso Department of Biochemistry-Microbiology University of D&#233dougou D&#233dougou Burkina Faso National Malaria Research and Training Centre (CNRFP) Ouagadougou Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Multidrug-Resistant Chicken Ouagadougou Escherichia coli Salmonella spp. Antibiotic

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