检索结果-南通市图书馆

Very Short-Term Probabilistic Prediction Method for Wind Speed Based on ALASSO-Nonlinear Quantile Regression and Integrated Criterion

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CSEE Journal of Power and Energy Systems 2023年第6期9卷 2121-2129页

作者： Yan Zhou Yonghui Sun Sen Wang linquan bai Dongchen Hou Rabea Jamil Mahfoud Peng Wang the College of Energy and Electrical Engineering Hohai UniversityNanjing 210098China Department of Systems Engineering and Engineering Management the University of North CarolinaCharlotte NC 28223USA

To enhance the performance of the prediction intervals (PIs), a novel very short-term probabilistic prediction method for wind speed via nonlinear quantile regression (NQR) based on adaptive least absolute shrinkage and selection operator (ALASSO) and integrated criterion (IC) is proposed. The ALASSO method is studied for shrinkage of output weights and selection of variables. Furthermore, for the better performance of PIs, composite weighted linear programming (CWLP) is proposed to modify the conventional linear programming cost function of quantile regression (QR), by combining it with Bayesian information criterion (BIC) as an IC to optimize the coefficients of PIs. Then, the multiple fold cross model (MFCM) is utilized to improve the PIs performance. Multistep probabilistic prediction of 15-minute wind speed is performed based on the real wind farm data from the northeast of China. The effectiveness of the proposed approach is validated through the performances' comparisons with conventional methods.

关键词： Composite weighted linear programming least absolute shrinkage and selection operator integrated criterion wind speed

Identification of multiple regulatory genes involved in TGase production in Streptomyces mobaraensis DSM 40587

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering Microbiology 2023年第4期3卷 20-28页

作者： Xian Liu Dan Wang Yuru Zhang Xiaoxin Zhuang linquan bai State Key Laboratory of Microbial Metabolism School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Joint International Research Laboratory of Metabolic&Developmental Sciences Shanghai Jiao Tong UniversityShanghai 200240China Taixing Dongsheng Bio-Tech Co. Ltd.Taixing 225411China

Microbial transglutaminase(TGase)is a protein that is secreted in a mature form and finds wide applications in meat products,tissue scaffold crosslinking,and textile *** mobaraensis is the only licensed producer of ***,increasing the production of TGase using metabolic engineering and heterologous expression approaches has encountered challenges in meeting industrial ***,it is necessary to identify the regulatory networks involved in TGase biosynthesis to establish a stable and highly efficient TGase cell *** this study,we employed a DNA-affinity capture assay and mass spectrometry analysis to discover several transcription *** the candidates,eight were selected and found to impact TGase ***,SMDS_4150,an AdpA-family regulator,exhibited a significant influence and was hence named *** electrophoretic mobility shift assays,we determined that AdpASm regulates TGase biosynthesis by directly repressing the transcription of tg and indirectly inhibiting the transcription of SMDS_*** latter gene encodes a LytR-family positive regulator of TGase ***,AdpASm exhibited negative regulation of its own *** further enhance TGase production,we combined the overexpression of SMDS_3961 with the repression of SMDS_4150,resulting in a remarkable improvement in TGase titer from 28.67 to 52.0 U/mL,representing an 81.37%*** study establishes AdpA as a versatile regulator involved in coordinating enzyme biosynthesis in Streptomyces ***,we elucidated a cascaded regulatory network governing TGase production.

关键词： Transglutaminase Streptomyces mobaraensis AdpA Transcription Regulatory network

Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2017年第9期60卷 1000-1009页

作者： Xiaojie Zhang Chenyang Lu linquan bai State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology Shanghai Jiao Tong University School of Marine Sciences Ningbo University

An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization,fast growth,abundant precursors and energy supply,and a pronounced gene *** albus BK3-25 is a high-yield industrial strain producing type-Ⅰ polyketide sahnomycin,with a unique ability of bean oil *** potential of being a surrogate host for heterologous production of PKS was engineered and evaluated ***,introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and sahnomycin,and subsequent deletion of the sahnomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors,including malonyl-CoA,methylmalonyl-CoA,and ***,the energy and reducing force were measured,and the improved accumulation of ATP and NADPH was observed in the ***,the strength of a series of selected endogenous promoters based on microarray data was assessed at different growth phases,and a strong constitutive promoter was identified,providing a useful tool for further engineered gene ***,the potential of the BK3-25 derived host ZXJ-6 was evaluated with the introduction of the actinorhodin biosynthetic gene cluster from Streptomyces coelicolor,and the heterologous production of actinorhodin was *** work clearly indicated the potential of the high-yield sahnomycin producer as a surrogate host for heterologous production of polyketides,although more genetic manipulation should be conducted to streamline its performance.

关键词：聚酮化合物白色链霉菌生产者主机代理生物合成基因簇盐霉素组成型启动子

Locational Marginal Pricing Mechanism for Uncertainty Management Based on Improved Multi-ellipsoidal Uncertainty Set

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2021年第4期9卷 734-750页

作者： Zongzheng Zhao Yixin Liu Li Guo linquan bai Chengshan Wang Key Laboratory of Smart Grid of Ministry of Education Tianjin UniversityTianjin 300072China Department of Systems Engineering and Engineering Man agement university of North Carolina at CharlotteCharlotteNC 28223USA

The large-scale integration of renewable energy sources (RESs) brings huge challenges to the power system. A cost-effective reserve deployment and uncertainty pricing mechanism are critical to deal with the uncertainty and variability of RES. To this end, this paper proposes a novel locational marginal pricing mechanism in day-ahead market for managing uncertainties from RES. Firstly, an improved multi-ellipsoidal uncertainty set (IMEUS) considering the temporal correlation and conditional correlation of wind power forecasting is formulated to better capture the uncertainty of wind power. The dimension of each ellipsoidal subset is optimized based on a comprehensive evaluation index to reduce the invalid region without large loss of modeling accuracy, so as to reduce the conservatism. Then, an IMEUS-based robust unit commitment (RUC) model and a robust economic dispatch (RED) model are established for the day-ahead market clearing. Both the reserve cost and ramping constraints are considered in the overall dispatch process. Furthermore, based on the Langrangian function of the RED model, a new locational marginal pricing mechanism is developed. The uncertainty locational marginal price (ULMP) is introduced to charge the RES for its uncertainties and reward the generators who provide reserve to mitigate uncertainties. The new pricing mechanism can provide effective price signals to incentivize the uncertainty management in the day-ahead market. Finally, the effectiveness of the proposed mechanism is verified via numerous simulations on the PJM 5-bus system and IEEE 118-bus system.

关键词： Day-ahead market ellipsoidal uncertainty set locational marginal pricing reserve robust unit commitment

Enhanced validamycin production and gene expression at elevated temperature in Streptomyces hygroscopicus subsp. jingangensis 5008

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第7期54卷 1204-1209页

作者： LI Lei bai linquan ZHOU XiuFen DENG ZiXin School of Life Science & Biotechnology and Laboratory of Microbial Metabolism Shanghai Jiao Tong University Shanghai 200030 China

Cultivation shift from 30℃ to 37℃ significantly enhanced validamycin (VAL) production. Analyzed by reverse-transcription PCR, the transcription of three val genes, valA, valK and valG, representing the three operons of the cluster was simultaneously increased at elevated temperature. Furthermore, the transcription of valP and valQ, a pair of two-component regulators in validamycin biosynthetic gene cluster, was also increased at 37℃. Inactivation of valP and valQ reduced validamycin production at 37℃ to the yield level of wild type strain at 30℃, and the val genes showed reduced expression in the mutant LL-8 at 37℃. These results revealed that the two-component regulator valP and valQ contribute to the elevated validamycin production.

关键词：有效霉素吸水链霉菌生产基因表达反转录聚合酶链反应高温生物合成基因簇转录基因

Inactivation of the positive LuxR-type oligomycin biosynthesis regulators OlmRI and OlmRII increases avermectin production in Streptomyces avermitilis

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2012年第8期57卷 869-876页

作者： YU Qing bai linquan ZHOU XiuFen DENG ZiXin State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200030China

Oligomycins are a group of 26 macrocyclic lactones that exhibit broad biological activities,including antifungal,anticancer and nematocidal *** of the oligomycin biosynthetic gene cluster (olm) in *** revealed 2 tandem LuxR-type regulators,OlmRI (931 aa) and OlmRII (941 aa),with shared identity of 38%.Gene replacement of olmRI or olmRII abolished oligomycin production,and this production could be partially restored in the disruptants by introducing cloned olmRI and olmRII with their native promoters,demonstrating the essential role of OlmRI and OlmRII for oligomycin *** real-time RT-PCR analysis revealed that transcription of 14 olm genes was differentially affected by the deletion of olmRI and ***,avermectin production in both mutants was enhanced at least *** identification of the positive cluster-situated regulators,OlmRI and OlmRII,paves the way for the transcriptional analysis of oligomycin biosynthesis and for the enhancement of oligomycin and avermectin production through regulator engineering.

关键词：生物合成基因簇阿维菌素生产监管 R型链霉菌 RT-PCR分析灭活

Day-ahead optimal scheduling method for grid-connected microgrid based on energy storage control strategy

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2016年第4期4卷 648-658页

作者： Xiangyu KONG linquan bai Qinran HU Fangxing LI Chengshan WANG Key Laboratory of Smart Grid of Ministry of Education Tianjin UniversityTianjin 300072China Department of Electrical Engineering and Computer Science University of TennesseeKnoxvilleTN 37996USA School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA

A day-ahead optimal scheduling method for a grid-connected microgrid based on energy storage(ES)control strategy is proposed in this *** proposed method optimally schedules ES devices to minimize the total operating costs while satisfying the load requirements of cold,heat,and electricity in *** modeling the operating cost function of each stage,the proposed method is able to adapt to different types of electricity markets and pricing *** technical characteristics of ES,such as self-discharge and round-trip efficiency,are considered in the control strategy with a multistage process *** improved dynamic programing method is used to solve the optimization ***,case studies are provided to illustrate the application process and verify the proposed method.

关键词： Energy storage Microgrid Optimal scheduling Market structure Price mechanism

Hybrid component and configuration model for combined-cycle units in unit commitment problem

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2018年第6期6卷 1332-1337页

作者： Xin FANG linquan bai Fangxing LI Bri-Mathias HODGE National Renewable Energy Laboratory ABB Inc. Department of EECS The University of Tennessee

This letter proposes a novel hybrid component and configuration model for combined-cycle gas turbines(CCGTs) participating in independent system operator(ISO) markets. The proposed model overcomes the inaccuracy issues in the current configuration-based model while retaining its simple and flexible bidding framework of configuration-based models. The physical limitations—such as minimum online/offline time and ramping rates—are modeled for each component separately, and the cost is calculated with the bidding curves from the configuration modes. This hybrid mode can represent the current dominant bidding model in the unit commitment problem of ISOs while treating the individual components in CCGTs accurately. The commitment status of the individual components is mapped to the unique configuration mode of the CCGTs. The transitions from one configuration mode to another are also modeled. No additional binary variables are added, and numerical case studies demonstrate the effectiveness of this model for CCGT units in the unit commitment problem.

关键词： Combined-cycle gas turbines(CCGTs) Unit commitment Component-based model Configurationbased model

A Dispatching Method for Integrated Energy System Based on Dynamic Time-interval of Model Predictive Control

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2020年第5期8卷 841-852页

作者： Xun Dou Jun Wang Zhen Wang Lijuan Li linquan bai Shuhui Ren Min Gao IEEE College of Electrical Engineering and Control Science Nanjing Tech UniversityNanjingChina Department of Systems Engineering and Engineering Management University of North Carolina at CharlotteCharlotteUSA Falcon Computing Solutions 10880 Wilshire Blvd Suite 1132Los AngelesCA 90024USA

In integrated energy systems(IESs),traditional fixed time-interval dispatching scheme is unable to adapt to the need of dynamic properties of the transient network,demand response characteristics,dispatching time scales in energy subsystems and renewable power *** scheme may easily result in uneconomic source-grid-load-storage operations in *** this paper,we propose a dispatching method for IES based on dynamic time-interval of model predictive control(MPC).We firstly build models for energy sub-systems and multi-energy loads in the power-gas-heat ***,we develop an innovative optimization method leveraging trajectory deviation control,energy control,and cost control frameworks in MPC to handle the requirements and constraints over the timeinterval of ***,a dynamic programming algorithm is introduced to efficiently solve the proposed *** and simulation results prove the effectiveness of the method.

关键词： Dispatching scheme dynamic time-interval integrated energy system model predictive control