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Quantification of microcystin-producing and non-microcystin producing Microcystis populations during the 2009 and 2010 blooms in Lake Taihu using quantitative real-time PCR

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Journal of Environmental Sciences 2012年第2期24卷 284-290页

作者： Daming Li Fanxiang Kong Xiaoli Shi linlin ye Yang Yu Zhen Yang State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Lake Taihu,a large,shallow hypertrophic freshwater lake in eastern China,has experienced lake-wide toxic cyanobacterial blooms annually during summer season in the past *** changes in the abundance of hepatotoxin microcystin-producing and nonmicrocystin producing Microcystis populations were investigated in the lake in August of 2009 and *** monitor the densities of the total Microcystis population and the potential microcystin-producing subpopulation,we used a quantitative real-time PCR assay targeting the phycocyanin intergenic spacer（PC-IGS） and the microcystin synthetase gene（mcyD）,*** the basis of quantification by real-time PCR analysis,the abundance of potential toxic Microcystis genotypes and the ratio of the mcyD subpopulation to the total Microcystis varied significantly,from 4.08×104 to 5.22×107 copies/mL,from 5.7% to 65.8%,*** analysis showed a strong positive relationship between chlorophyll-a,toxic Microcystis and total Microcystis;the abundance of toxic Microcystis correlated positively with total phosphorus and ortho-phosphate concentrations,but negatively with TN：TP ratio and nitrate *** the proportion of potential toxic genotypes within Microcystis population showed positive correlation with total phosphorus and ortho-phosphate *** data suggest that increased phosphorus loading may be a significant factor promoting the occurrence of toxic Microcystis bloom in Lake Taihu.

关键词： microcystin-producing Microcystis real-time PCR toxic cyanobacterial bloom microcystin synthetase gene cluster

Specific protein expression in a rat model of early focal cerebral ischemia:Fluorescent two-dimensional difference gel electrophoresis

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Neural Regeneration Research 2010年第3期5卷 209-213页

作者： Xueling Ma Wei Yang Xinmei Jiang Fuchun Li Xia Li linlin ye Kangding Liu Department of Neurology the Fourth Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China Department of Neurology the First Hospital of Jilin University Changchun 130021 Jilin Province China Department of Orthopedics the First Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China

BACKGROUND： The use of fluorescent two-dimensional difference gel electrophoresis （2D-DIGE） has been shown to compensate for the shortcomings of conventional two-dimensional gel electrophoresis, such as poor repeatability and large systematic errors. However, little information is presently available regarding the use of 2D-DIGE to investigate mechanisms of ischemic cerebrovascular diseases. Plasma and body fluids have been utilized in proteomic technology to study ischemic cerebrovascular diseases. OBJECTIVE： To perform proteomic analysis of fresh rat brain tissue in peripheral ischemic regions using 2D-DIGE 6 hours after middle cerebral artery occlusion （MCAO）, and to identify specific proteins closely associated with early ischemic cerebrovascular diseases. DESIGN, TIME AND SETTING： Proteomics-based, randomized, controlled, animal experiment was performed at the Laboratories of Neurology and Proteomics, Jilin University between January and April 2006. MATERIALS： 2, 3, 5-triphenyl tetrazolium chloride was purchased from Sigma, USA. Ettan DALTSix system, DeCyder DIA V5.0 differential analysis software, and Ettan matrix-assisted laser desorption/ionization time-of-flight mass spectrometer （MALDI-TOF-MS） were purchased from Amersham Bioscience, Sweden. METHODS： Eight healthy, male, Wistar rats were randomized to experimental and control groups, with four rats in each group. In the experimental group, rat models of focal cerebral ischemia were established by MCAO. In the control group, the internal and external carotid arteries were exposed and then immediately sutured, and the remaining procedures were identical to the experimental group. MAIN OUTCOME MEASURES： At 6 hours after cerebral ischemia, protein expression in the peripheral ischemia region of the experimental group was compared with the control group using 2D-DIGE. Protein spots that exhibited statistical differences between experimental and control groups with 〉 1.4 attributable risk were screened using DeC

关键词： fluorescent two-dimensional difference gel electrophoresis cerebral ischemia mass spectrometry rats proteomics neural regeneration

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Journal of Integrative Agriculture 2024年第11期23卷 3788-3800页

作者： Chengdong Yang Manfei Luo Xue Zhang linlin ye Ge Yu Yi Lü Yi Chen Taixu Chen Xuejian Wang Wanzhen Feng Qinghe Chen School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication) Hainan UniversitySanya 572025China Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education School of Tropical Agriculture and ForestryHainan UniversityHaikou 570228China Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Fuzhou 350013China

Litchi downy blight,caused by the plant pathogenic oomycete Peronophythora litchii,is one of the most devastating diseases on litchi and resulted in huge economic *** plays an essential role in the development and pathogenicity of the filamentous ***,the function of autophagy in oomycetes remain ***,an autophagy-related protein Atg3 homolog PlAtg3 was identified and characterized in *** absence of PlATG3 through the CRISPR/Cas9 gene replacement strategy compromised vegetative growth and sexual/asexual *** analyses revealed that the deletion of PlATG3 impaired autophagosome formation with monodansylcadaverine(MDC)staining and significantly disrupted zoospore release due to defects of sporangial cleavage with FM4-64 ***8 is considered to be an autophagy marker protein in various *** blot analysis indicated that PlAtg3 is involved in degradation of ***,PlAtg3 was unable to interact with PlAtg8 in yeast two hybrid(Y2H)assays,possibly due to the absence of the Atg8 family interacting motif(AIM)in ***,pathogenicity assays revealed that the deletion of PlATG3 considerably reduced the virulence of *** together,our data reveal that PlAtg3 plays an important role in radial growth,asexual/sexual development,sporangial cleavage and zoospore release,autophagosome formation,and pathogenicity in *** study contributes to a better understanding of the pathogenicity mechanisms of *** and provides insights for the development of more effective strategies to control oomycete diseases.

关键词： PlAtg3 sporangial cleavage autophagy pathogenicity Peronophythora litchii

Temporal and spatial variation of cloud cover in arid regions of Central Asia in the last 40 years

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Research in Cold and Arid Regions 2023年第2期15卷 66-72页

作者： KaiLiang Zhao GuoFeng Zhu LiYuan Sang JiaWei Liu Lei Wang YuWei Liu YuanXiao Xu XinRui Lin WenHao Zhang linlin ye College of Geography and Environmental Science Northwest Normal UniversityLanzhouGansu 730070China Shiyang River Ecological Environment Observation Station Northwest Normal UniversityLanzhouGansu 730070China Lanzhou Sub-Center Remote Sensing Application CenterMinistry of AgricultureLanzhouGansu 730000China

Water resources are one of the key factors restricting the development of arid areas,and cloud water resources is an important part of water *** arid region of central Asia is the core region of the current national green silk road construction,and is the largest arid region in the *** on cloud cover data of ECMWF,the current study analyzed temporal and spatial characteristics of cloud properties in arid regions of Central Asia between 1980 and *** findings show that:(1)From the point of view of spatial distribution,total cloudiness in arid regions of Central Asia was low in the south and high in the *** distribution of high cloud frequency and medium cloud frequency was higher in the south and lower in the north,while low cloud frequency distribution was low in the south and high in the north.(2)In terms of time,the variation of cloud cover and cloud type frequency had obvious seasonal *** winter to spring,cloud cover increased,and the change of cloud type frequency *** spring to summer,cloud cover continued to increase and the change of cloud type frequency increased *** cover began to decrease from summer to autumn,and the change of cloud type frequency also decreased.(3)Generally,average total cloud cover decreased in most of central Asia,and high and medium cloud cover increased while low cloud cover *** study provides a reference for the rational development of cloud resources in the region.

关键词： Central Asia Cloud cover Temporal and spatial changes Cloud type frequency

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中国城市化与居民储蓄研究

经济问题探索 2009年第2期 7-12页

作者：叶琳琳中央财经大学中国经济与管理研究院北京100081

本文利用1992年-2004年的数据对我国居民储蓄进行了实证研究,力求在居民储蓄率、城市化程度的测量两个方面体现出新意。本文研究表明,目前我国城市化的程度有所高估。在城市化过程中,居民储蓄率受影响较大,进而影响全国国民储蓄和投资,... 详细信息

本文利用1992年-2004年的数据对我国居民储蓄进行了实证研究,力求在居民储蓄率、城市化程度的测量两个方面体现出新意。本文研究表明,目前我国城市化的程度有所高估。在城市化过程中,居民储蓄率受影响较大,进而影响全国国民储蓄和投资,减缓我国经济的增速。但随着城市化的不断深入,这种影响将逐渐削弱。

关键词：生命周期理论居民储蓄率城市化

基于集合经验分解与改进阈值函数的小波变换心电信号去噪方法研究

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生物医学工程学杂志 2014年第3期31卷 567-571页

作者：叶琳琳杨丹王旭东北大学信息科学与工程学院沈阳110819

针对心电图(ECG)信号去噪问题,提出了一种基于集合经验分解(EEMD)和改进阈值函数的小波变换去噪方法。首先利用EEMD对含噪的ECG信号进行分解,选取固有模态函数(IMF),重构ECG信号,实现ECG信号的一次去噪;再利用改进阈值函数的小波变换方... 详细信息

针对心电图(ECG)信号去噪问题,提出了一种基于集合经验分解(EEMD)和改进阈值函数的小波变换去噪方法。首先利用EEMD对含噪的ECG信号进行分解,选取固有模态函数(IMF),重构ECG信号,实现ECG信号的一次去噪;再利用改进阈值函数的小波变换方法对ECG信号进一步去噪。实验中,利用MIT-BIH心电图数据库对提出的方法进行评估,用参数信噪比(SNR)和均方误差(MSE)比较EEMD、改进阈值函数的小波变换方法以及本文提出的方法的去噪效果。实验结果表明:本文提出的方法去噪后的ECG信号波形平滑,特征点幅值无衰减,在去噪的同时更好地保留了原始ECG信号的特征。

关键词：心电图集合经验分解固有模态函数改进阈值函数 MIT—BIH

灰色MGM(1,m,N)模型的构建及其在雾霾预测中的应用

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系统工程理论与实践 2020年第3期40卷 771-782页

作者：熊萍萍袁玮莹叶琳琳邹俊秀南京信息工程大学管理工程学院南京210044 南京信息工程大学数学与统计学院南京210044

针对传统MGM(1,m)模型和GM(1,N)模型均未能反映多个系统行为变量在多个因素变量影响下的模拟预测问题,本文根据两个模型各自特点对传统MGM(1,m)模型和GM(1,N)模型进行拓展,构建了灰色MGM(1,m,N)模型.研究该模型的建模机理及过程,并解决... 详细信息

针对传统MGM(1,m)模型和GM(1,N)模型均未能反映多个系统行为变量在多个因素变量影响下的模拟预测问题,本文根据两个模型各自特点对传统MGM(1,m)模型和GM(1,N)模型进行拓展,构建了灰色MGM(1,m,N)模型.研究该模型的建模机理及过程,并解决在多个因素变量的影响下多个系统行为变量的模拟预测问题.最后,将三种模型应用于雾霾的模拟预测中,结果表明,MGM(1,m,N)模型预测精度高于传统的MGM(1,m)模型和GM(1,N)模型,这主要是由于该模型能够较好地描述和反映多个系统行为变量受多个因素变量的影响.

关键词：灰色MGM(1,m,N)模型行为变量因素变量雾霾预测

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基于蛇优化算法的Otsu图像分割方法

国外电子测量技术 2023年第2期42卷 30-37页

作者：李圣涵叶琳琳海南大学儋州571700

Otsu算法是图像处理中运用广泛的图像分割方法,尽管有着计算简单、准确的特性,但因为需要进行穷举运算,所以计算效率不高。为提高图像分割的实时性,引入了蛇优化算法(SO)对Otsu进行了优化,创建了基于蛇优化算法的Otsu图像分割方法(SO-Ot... 详细信息

Otsu算法是图像处理中运用广泛的图像分割方法,尽管有着计算简单、准确的特性,但因为需要进行穷举运算,所以计算效率不高。为提高图像分割的实时性,引入了蛇优化算法(SO)对Otsu进行了优化,创建了基于蛇优化算法的Otsu图像分割方法(SO-Otsu)。在该算法中,利用蛇优化算法来模拟蛇的特性进行最佳阈值的寻找,以降低迭代时间,提升计算速度。在仿真实验中,利用经典的Lena、Peppers、Goldhill、Cameraman图片进行测试,与基于果蝇优化算法的Otsu方法(FOA-Otsu)和基于麻雀搜索算法的Otsu方法(SSA-Otsu)进行对比。并通过计算峰值信噪比(PSNR)、结构相似性(SSIM)、特征相似性(FSIM)和计算时间作为评价指标结果进行评估。结果表明,与其他算法相比,算法计算效率高、分割细节效果好且综合分割性能优异,为提高图像分割的计算效率提供了一种理想的工具。

关键词： Otsu 蛇优化算法图像分割

竹叶发酵液的美白、增强皮肤屏障和抗炎护肤功效研究

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高校化学工程学报 2023年第4期37卷 591-598页

作者：叶琳琳陈志雄阮建成钱超杭州雅妍化妆品有限公司浙江杭州311123 浙江大学化学工程与生物工程学院浙江杭州310058

为研究竹叶发酵液(FBL)的护肤功效,选取角质形成细胞(HaCaT)和黑色素瘤细胞(B16)作为细胞模型,利用细胞计数试剂(CCK-8)评估竹叶发酵液的细胞毒性;并通过检测黑色素含量、十二烷基硫酸钠(SLS)损伤模型下丝聚蛋白和炎症因子的基因表达,... 详细信息

为研究竹叶发酵液(FBL)的护肤功效,选取角质形成细胞(HaCaT)和黑色素瘤细胞(B16)作为细胞模型,利用细胞计数试剂(CCK-8)评估竹叶发酵液的细胞毒性;并通过检测黑色素含量、十二烷基硫酸钠(SLS)损伤模型下丝聚蛋白和炎症因子的基因表达,分别考察FBL的美白、增强皮肤屏障和抗炎的护肤功效。结果表明,FBL对上述细胞均没有毒性,同时能够显著降低B16细胞内黑色素的含量(显著性p<0.001),并能促进丝聚蛋白的基因表达(p<0.001)、抑制肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的基因表达(p<0.001,p<0.01)。因此,FBL具有潜在的护肤功效。

关键词：竹叶发酵液美白皮肤屏障抗炎