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Freezing-directed construction of enzyme/nano interfaces:Reagentless conjugation,superior activity,and better stability

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Chinese Chemical Letters 2024年第1期35卷 471-474页

作者： Ke Quan Jiajie Tong Lifang Chen Shuyao Fang Mengjiao Li linlin wu Zhihe Qing Hunan Provincial Key Laboratory of Cytochemistry School of Food and BioengineeringSchool of Chemistry and Chemical EngineeringChangsha University of Science and TechnologyChangsha 410114China Department of Oncology Tengzhou Central People’s Hospital Affiliated Xuzhou Medical UniversityTengzhou 277500China

Immobilizing enzyme to nano interfaces has demonstrated to be a favorable strategy for prompting the industrialized application of enzyme.Despite tremendous endeavor has been devoted to using gold nanoparticles(AuNPs)as conjugation matrix due to its fascinating physico-chemical properties,maintaining enzymatic activity while circumventing cumbersome modification remains a formidable challenge.Herein,the freezing-directed conjugation of enzyme/nano interfaces was constructed without extra reagent.As the proof of concept,glucose oxidase(GOx)was chosen as model enzyme.The one-pot conjugation process can be facilely completed at−20°C under aqueous solution.Moreover,with the loading of GOx on AuNP at freezing,the enzyme exhibited superior catalytic activity and stability upon thermal and pH perturbation.The mechanism of boosted activity was then discussed in detail.It was found that higher loading density under freezing condition and more enzyme tending to bind AuNPs via Au-S bond were the main factors for the superior activity.More importantly,this methodology was universal and can also be applied to other enzyme which contains natural cysteine,such as horseradish peroxidase(HRP)and papain.This facile conjugation strategy accompanied by remarkable bioactivity expand the possibilities for enzymatic biosensing,microdevice and even drug delivery.

关键词： Enzyme conjugation Freezing AuNPs Catalytic activity

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Dynamic Multi-Attribute Decision Analysis on Three-Parameter Interval Grey Number of Upper(Lower)Deviation Quasi-Normal Distribution

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Journal of Systems Science and Information 2022年第3期10卷 280-296页

作者： Fenyi DONG linlin wu Han SHEN College of Information and Management Science Henan Agricultural UniversityZhengzhou 450046China

Aiming at the dynamic multi-attribute decision making problem where the weight of each decision stage and attribute weight are completely unknown and the attribute value is unknown distributed three-parameter interval grey number,a threeparameter interval grey number dynamic multiattribute grey target decision making method with attribute value following quasi-normal distribution is proposed.Firstly,the position relationship between the“center of gravity”point and the kernel of the threeparameter interval grey number is discussed.According to the characteristicthat the attribute value obeys the quasi-normal distribution,anew weight isgiventothe“centerof gravity”point,and a new distance measure formula of the three-parameter interval grey number is defined.Secondly,according to the principle of maximum entropy,the objective programming model is constructed to determine the stage weight and attributeweight.Then,the schemes aresorted according to thesize of the comprehensive bull's-eye distance Finally an example is given to illustrate the effectiveness of the decision model.

关键词： three-parameter interval grey number grey target decision making quasi-normal distribution multiple attribute decision making

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Excessive gestational weight gain in early pregnancy and insufficient gestational weight gain in middle pregnancy increased risk of gestational diabetes mellitus

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Chinese Medical Journal 2022年第9期135卷 1057-1063页

作者： Aiqi Yin Fuying Tian Xiaoxia wu Yixuan Chen Kan Liu Jianing Tong Xiaonian Guan Huafan Zhang linlin wu Jianmin Niu Department of Obsetics Shenchen Matemity and Chid Helthare HoptalThe First School of Cinial MedicneSouthem Medcal UnierityShenzhenGuangdong 518028China

Background:Gestational weight gain(GWG)is associated with the risk of gestational diabetes mellitus(GDM).However,the effect of weight gain in different trimesters on the risk of GDM is unclear.This study aimed to evaluate the effect of GWG on GDM during different trimesters.Methods:A birth cohort study was conducted from 2017 to 2020 in Shenzhen,China.In total,51,205 participants were included comprising two models(early pregnancy model and middle pregnancy model).Gestational weight(kg)was measured at each prenatal clinical visit using a standardized weight scale.Logistic regression analysis was used to assess the risk of GDM.Interaction analysis and mediation effect analysis were performed in the middle pregnancy model.Results:In the early pregnancy model,the risk of GDM was 0.858 times lower(95%confidence interval[CI]:0.786,0.937)with insufficient GWG(iGWG)and 1.201 times higher(95%CI:1.097,1.316)with excessive GWG after adjustment.In the middle pregnancy model,the risk of GDM associated with iGWG increased 1.595 times(95%CI:1.418,1.794)after adjustment;for excessive GWG,no significant difference was found(P=0.223).Interaction analysis showed no interaction between GWG in early pregnancy(GWG-E)and GWG in middle pregnancy(GWG-M)(F=1.268;P=0.280).The mediation effect analysis indicated that GWG-M plays a partial mediating role,with an effect proportion of 14.9%.Conclusions:eGWG-E and iGWG-M are associated with an increased risk of GDM.Strict control of weight gain in early pregnancy is needed,and sufficient nutrition should be provided in middle pregnancy.

关键词： Gestational diabetes mellitus Gestational weight gain Early pregnancy Middle pregnancy

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QpefBD:A Benchmark Dataset Applied to Machine Learning for Minute-Scale Quantitative Precipitation Estimation and Forecasting

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Journal of Meteorological Research 2022年第1期36卷 93-106页

作者： Anyuan XIONG Na LIU Yujia LIU Shulin ZHI linlin wu Yongjian XIN Yan SHI Yunjian ZHAN National Meteorological Information Center China Meteorological AdministrationBeijing 100081 Jiangxi Meteorological Observatory Nanchang 330096 Anhui Weather Modification Office Hefei 230061

Nowcasts of strong convective precipitation and radar-based quantitative precipitation estimations have always been hot yet challenging issues in meteorological sciences.Data-driven machine learning,especially deep learning,provides a new technical approach for the quantitative estimation and forecasting of precipitation.A high-quality,large-sample,and labeled training dataset is critical for the successful application of machine-learning technology to a specific field.The present study develops a benchmark dataset that can be applied to machine learning for minutescale quantitative precipitation estimation and forecasting(QpefBD),containing 231,978 samples of 3185 heavy precipitation events that occurred in 6 provinces of central and eastern China from April to October 2016-2018.Each individual sample consists of 8 products of weather radars at 6-min intervals within the time window of the corresponding event and products of 27 physical quantities at hourly intervals that describe the atmospheric dynamic and thermodynamic conditions.Two data labels,i.e.,ground precipitation intensity and areal coverage of heavy precipitation at 6-min intervals,are also included.The present study describes the basic components of the dataset and data processing and provides metrics for the evaluation of model performance on precipitation estimation and forecasting.Based on these evaluation metrics,some simple and commonly used methods are applied to evaluate precipitation estimates and forecasts.The results can serve as the benchmark reference for the performance evaluation of machine learning models using this dataset.This paper also gives some suggestions and scenarios of the QpefBD application.We believe that the application of this benchmark dataset will promote interdisciplinary collaboration between meteorological sciences and artificial intelligence sciences,providing a new way for the identification and forecast of heavy precipitation.

关键词： machine learning benchmark dataset quantitative precipitation estimation precipitation forecast

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Which fetal growth charts should be used?A retrospective observational study in China

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Chinese Medical Journal 2022年第16期135卷 1969-1977页

作者： Jianxin Zhao Ying Yuan Jing Tao Chunyi Chen Xiaoxia wu Yimei Liao linlin wu Qing Zeng Yin Chen Ke Wang Xiaohong Li Zheng Liu Jiayuan Zhou Yangwen Zhou Shengli Li Jun Zhu National fficefor Maternal and Child HealthSurvillance of China West China Second University HospitalSichuan UniversityChengduSichuan 610041China Department of Ultrasound Shenzhen Maternity and Chid Healthcare HospitalSouthern Medical UniversityShenzhenGuangdong 518028China Department of Obstetics Shenzhen Maternity and Child Healthcare HospitalSouthern Medical UniversityShenzhenGuangdong 518028China key Laboratoryof Birth Defects and Related Diseases of Women and Chidren(Sichuan University) Ministry of EducationWest China Second University HospitalSichuan UniversityChengduSichuan 610041China sichuan Birth Defects Clinical Research Center West China Second University HospitalSichuan UniversityChengduSichuan 610041China

Background:The fetal growth charts in widest use in China were published by Hadlock>35 years ago and were established on data from several hundred of American pregnant women.After that,>100 fetal growth charts were published around the world.We attempted to assess the impact of applying the long-standing Hadlock charts and other charts in a Chinese population and to compare their ability to predict newborn small for gestational age(SGA).Methods:For this retrospective observational study,we reviewed all pregnant women(n=106,455)who booked prenatal care with ultrasound measurements for fetal biometry at the Shenzhen Maternity and Child Healthcare Hospital between 2012 and 2019.A fractional polynomial regression model was applied to generate Shenzhen fetal growth chart ranges for head circumference(HC),biparietal diameter(BPD),abdominal circumference(AC),and femur length(FL).The differences between Shenzhen charts and published charts were quantified by calculating the Z-score.The impact of applying these published charts was quantifed by calculating the proportions of fetuses with biometric measurements below the 3rd centile of these charts.The sensitivity and area under the receiver operating characteristic curves of published charts to predict neonatal SGA(birthweight<10th centile)were assessed..Results:Following selection,169,980 scans of fetal biometry contributed by 41,032 pregnancies with reliable gestational age were analyzed.When using Hadlock references(<3rd centile),the proportions of small heads and short femurs were as high as 8.9%and 6.6%in late gestation,respectively.The INTERGROWTH-21st standards matched those of our observed curves better than other charts,in particular for fat-free biometry(HC and FL).When using AC<10th centile,all of these references were poor at predicting neonatal SGA.Conclusions:Applying long-standing Hadlock references could misclassify a large proportion of fetuses as SGA.INTERGROWTH-21st standard appears to be a safe option in China.For fat-based biometry,AC,a reference base

关键词： Infant newborn Pregnancy Growth chart Gestational age Birth weight Prenatal care Biometry Fetal growth reference Ultrasound measurement Hadlock charts INTERGROWTH-21st charts China

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Schisandra lignans ameliorate nonalcoholic steatohepatitis by regulating aberrant metabolism of phosphatidylethanolamines

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Acta Pharmaceutica Sinica B 2023年第8期13卷 3545-3560页

作者： Lijuan Xue Keanqi Liu Caixia Yan Junling Dun Yexin Xu linlin wu Huizhu Yang Huafang Liu Lin Xie Guangji Wang Yan Liang Key Lab of Drug Metabolism&Pharmacokinetics State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China Zhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research Department of Clinical Pharmacythe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Analytical Applications Center Shimadzu(China)Co.Ltd.Shanghai 200233China

Nonalcoholic steatohepatitis(NASH)is a spectrum of chronic liver disease characterized by hepatic lipid metabolism disorder.Recent reports emphasized the contribution of triglyceride and diglyceride accumulation to NASH,while the other lipids associated with the NASH pathogenesis remained unexplored.The specific purpose of our study was to explore a novel pathogenesis and treatment strategy of NASH via profiling the metabolic characteristics of lipids.Herein,multi-omics techniques based on LC—Q-TOF/MS,LC—MS/MS and MS imaging were developed and used to screen the action targets related to NASH progress and treatment.A methionine and choline deficient(MCD)diet-induced mouse model of NASH was then constructed,and Schisandra lignans extract(SLE)was applied to alleviate hepatic damage by regulating the lipid metabolism-related enzymes CES2A and CYP4A14.Hepatic lipidomics indicated that MCD-diet led to aberrant accumulation of phosphatidylethanolamines(PEs),and SLE could significantly reduce the accumulation of intrahepatic PEs.Notably,exogenous PE(18:0/18:1)was proved to significantly aggravate the mitochondrial damage and hepatocyte apoptosis.Supplementing PE(18:0/18:1)also deteriorated the NASH progress by up regulating intrahepatic proinflammatory and fibrotic factors,while PE synthase inhibitor exerted a prominent hepatoprotective role.The current work provides new insights into the relationship between PE metabolism and the pathogenesis of NASH.

关键词： Nonalcoholic steatohepatitis Multi-omics Schisandra lignans extract Phosphatidyle thanolamine PE(18:0/18:1)

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Prediction of the Maturity of Greenhouse Grapes Based on Imaging Technology

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Plant Phenomics 2022年第1期4卷 357-370页

作者： Xinguang Wei linlin wu Dong Ge Mingze Yao Yikui Bai College of Water Conservancy Shenyang Agricultural UniversityShenyang 110866China Institute of Soil and Water Conservation Northwest A&F University712100YanglingShaanxi ProvinceChina

To predict grape maturity in solar greenhouses,a plant phenotype-monitoring platform(Phenofix,France)was used to obtain RGB images of grapes from expansion to maturity.Horizontal and longitudinal diameters,compactness,soluble solid content(SSC),titratable acid content,and the SSC/acid of grapes were measured and evaluated.The color values(R,G,B,H,S,andI)of the grape skin were determined and subjected to a back-propagation neural network algorithm(BPNN)to predict grape maturity.

关键词： maturity greenhouse content

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Multi-Branch Cable Harness Layout Design Based on Genetic Algorithm with Probabilistic Roadmap Method

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Chinese Journal of Mechanical Engineering 2021年第2期34卷 68-78页

作者： Yingfeng Zhao Jianhua Liu Jiangtao Ma linlin wu School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China

Current studies on cable harness layouts have mainly focused on cable harness route planning.However,the topological structure of a cable harness is also extremely complex,and the branch structure of the cable harness can affect the route of the cable harness layout.The topological structure design of the cable harness is a key to such a layout.In this paper,a novel multi-branch cable harness layout design method is presented,which unites the probabilistic roadmap method(PRM)and the genetic algorithm.First,the engineering constraints of the cable harness layout are presented.An obstacle-based PRM used to construct non-interference and near to the surface roadmap is then described.In addition,a new genetic algorithm is proposed,and the algorithm structure of which is redesigned.In addition,the operation probability formula related to fitness is proposed to promote the efficiency of the branch structure design of the cable harness.A prototype system of a cable harness layout design was developed based on the method described in this study,and the method is applied to two scenarios to verify that a quality cable harness layout can be efficiently obtained using the proposed method.In summary,the cable harness layout design method described in this study can be used to quickly design a reasonable topological structure of a cable harness and to search for the corresponding routes of such a harness.

关键词： Cable harness layout Probabilistic roadmap method Genetic algorithm Hybrid fuzzy control

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共同体视域下两岸融合发展话语体系分析

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厦门大学学报（哲学社会科学版） 2024年第2期74卷 95-106页

作者：吴琳琳文常莹厦门大学新闻传播学院福建厦门361005

两岸融合发展话语不仅是表现两岸命运共同体的政治话语实践,而且是在意义方面说明、组成和建构两岸命运共同体理念。通过共词分析的方法,共同体视域下两岸融合发展话语体系呈现出从抽象到具体的话语发展逻辑,以及从中央到地方的话语演... 详细信息

两岸融合发展话语不仅是表现两岸命运共同体的政治话语实践,而且是在意义方面说明、组成和建构两岸命运共同体理念。通过共词分析的方法,共同体视域下两岸融合发展话语体系呈现出从抽象到具体的话语发展逻辑,以及从中央到地方的话语演进路径。整体来看,两岸融合发展话语的建构以马克思共同体理论为指导,以习近平总书记系列讲话为指引,结合中央政策与地方政策两个话语层次,进而立体、全面地建构两岸命运共同体。两岸融合发展话语作为共同体理论在实现国家完全统一方面的话语应用与实践,不仅进一步延伸与发展了两岸融合发展实践,同时也为人类命运共同体提供有益的借鉴。

关键词：共同体两岸融合发展话语体系

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非酒精性脂肪性肝病伴肥胖患者血清25羟维生素D的表达及其临床意义

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临床肝胆病杂志 2024年第7期40卷 1349-1353页

作者：武琳琳张帆山西医科大学微生物学与免疫学教研室太原030001 山西白求恩医院(山西医学科学院) 山西医科大学第三医院同济山西医院检验科太原030032

目的检测非酒精性脂肪性肝病(NAFLD)伴肥胖患者血清25羟维生素D的表达水平,分析25羟维生素D与机体肝功能、血脂及炎症指标的关系。方法选取2022年1月—2023年3月于山西白求恩医院就诊的NAFLD患者90例,根据BMI分为NAFLD伴肥胖组60例(BMI... 详细信息

目的检测非酒精性脂肪性肝病(NAFLD)伴肥胖患者血清25羟维生素D的表达水平,分析25羟维生素D与机体肝功能、血脂及炎症指标的关系。方法选取2022年1月—2023年3月于山西白求恩医院就诊的NAFLD患者90例,根据BMI分为NAFLD伴肥胖组60例(BMI≥28 kg/m^(2))和NAFLD组30例(BMI<28 kg/m^(2));另选取同期健康体检者30例作为对照组。检测3组人群血清25羟维生素D、肝功能指标(ALT、AST、ALP、GGT、TBil、DBil)、血脂指标(HDL、LDL、TC、TG)、炎症指标(H-CRP、GP-73)以及细胞因子水平(IL-2、IL-4、IL-6、IL-10、IL-17、IL-1β、TNF-α、IFN-γ),测量肝脾体积比值。正态分布的计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验或Tamhane’s T2法;非正态分布的计量资料多组间比较及进一步两两比较均采用Kruskal-Wallis H检验。相关性采用Pearson或Spearman相关性分析。结果与对照组相比,NAFLD伴肥胖组25羟维生素D,HDL,细胞因子IL-2、IL-4、IL-10、IFN-γ和肝脾体积比值均显著降低(P值均<0.05),而肝功能指标ALT、AST、ALP、GGT、TBil、DBil,血脂指标LDL、TC、TG,炎症指标H-CRP、GP-73及细胞因子IL-1β、IL-17、TNF-α均明显升高(P值均<0.05)。NAFLD伴肥胖组与NAFLD组比较,除肝脾体积比值和H-CRP水平外,上述指标差异均有统计学意义(P值均<0.05)。相关性分析结果显示,25羟维生素D水平与ALT(r=-0.324,P=0.012)、AST(r=-0.421,P=0.001)、ALP(r=-0.435,P=0.001)、GGT(r=-0.343,P=0.007)、TBil(r=-0.532,P<0.001)、DBil(r=-0.521,P<0.001)、LDL(r=-0.405,P=0.001)、TC(r=-0.466,P<0.001)、TG(r=-0.551,P<0.001)、H-CRP(r=-0.434,P=0.014)、GP-73(r=-0.421,P=0.001)、IL-1β(r=-0.433,P=0.001)、IL-17(r=-0.465,P<0.001)和TNF-α(r=-0.533,P<0.001)呈负相关;与HDL(r=0.632,P<0.001)、IL-2(r=0.546,P<0.001)、IL-4(r=0.533,P<0.001)、IL-10(r=0.456,P<0.001)及肝脾体积比值(r=0.543,P<0.001)呈正相关。结论NAFLD伴肥胖患者血清25羟维生素D与肝功能、血脂及炎症指标均存在显著相关性,25羟维生素D可能通过减轻炎症反应缓解NAFLD伴肥胖患者症状,为NAFLD治疗提供新的干预策略。

关键词：非酒精性脂肪性肝病肥胖症 25羟维生素D

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