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Evaluation of Drought Stress-Inducible W<i>si</i>18 Promoter in <i>Brachypodium distachyon</i>

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Advances in Bioscience and Biotechnology 2018年第11期9卷 596-612页

作者： Patrick Langille Wei Wei Jim Karagiannis Tim Xing lining tian Department of Biology University of Western Ontario London Canada London Research and Development Centre Agriculture and Agri-Food Canada London Canada State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control Institute of Quality and Standard for Agro-Products Zhejiang Academy of Agricultural Sciences Hangzhou China Department of Biology Carleton University Ottawa Canada

The rice Wsi18 promoter confers drought-inducible gene expression. This property makes it a useful candidate to drive relevant genes for developing drought resistant traits for different monocot crops. In this study, we showed that the Bradi2G47700 gene, the closest homologue to rice Wsi18, was upregulated in Brachypodium distachyon plants exposed to ABA and mannitol. Wsi18: uidA transgenic B. distachyon plants were produced and then subjected to ABA or mannitol treatment. The expression of uidA in three transgenic lines (line 10, 18 and 37) was significantly upregulated in plants exposed to ABA (fold increases of 5.61 ± 0.98, 2.88 ± 0.75 and 9.13 ± 1.96, respectively) compared to the same transgenic plant lines without treatment. The expression of uidA in two transgenic lines (lines 18 and 37) also showed upregulation when treated with mannitol (fold increases of 4.43 ± 1.07 and 8.47 ± 2.90, respectively) compared to the same transgenic plant lines without mannitol treatment. Moreover, GUS histochemical assay showed increased Wsi18 promoter activity in the leaves and stems of transgenic lines upon treatment with ABA or mannitol. This is the first report of the drought inducible rice Wsi18 promoter being active in B. distachyon which is a model plant for molecular biology research of various monocot plants. Taken together, the results indicate that the Wsi18 promoter and its homologue may be explored as a useful tool for drought stress-inducible gene expression in different monocot crops.

关键词： Wsi18 Promoter Drought Inducible Brachypodium distachyon ABA Mannitol

Recent Progress of Transgenic Technology Development for Alfalfa

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American Journal of Plant Sciences 2018年第3期9卷 467-482页

作者： Wenting Liu Banyar Aung Abdelali Hannoufa Tim Xing lining tian College of Life Science Northwest A&F University Yangling China London Research and Development Center Agriculture and Agri-Food Canada London Canada Department of Biology Carleton University Ottawa Canada

Alfalfa (Medicago sativa L.) is an important leguminous crop worldwide and it has important roles in different aspects of the agriculture system, including livestock feed, crop soil conservation, and improvement of soil nitrogen supply. As a perennial crop, alfalfa breeding for trait improvement takes longer periods of time compared to many other crops, therefore, genetic engineering is a faster route for alfalfa trait modification and improvement. Alfalfa was a first crop in which somatic embryogenesis was developed. Alfalfa was also a pioneering crop in which transgenic technology was developed and applied for trait improvement. Transgenic technology in alfalfa has since advanced steadily in various areas. In this review, we update the recent research progress in alfalfa genetic engineering, focusing on genetic transformation and use of transgenic technology for trait improvement and new trait development.

关键词： Medicago sativa Transformation Tissue Culture

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大通县老爷山植物资源及其保护利用

青海师范大学学报(自然科学版) 1987年第4期 63-66页

作者：田立宁青海师院大学生物系

老爷山地区植物资源较丰富。据初步调查,共有维管植物274种。目前,其植物资源受人为影响和干扰较大,利用强度也很低。对该地植物资凉应本着保护与利用相结合的原则,使其发挥更大效益。

关键词：老爷山植物资源生物资源山杨林珠芽鳞茎马先唐松草美蔷薇大通县

高分子有机膜亲水改性及光催化改性的研究进展

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环境科学与技术 2017年第6期40卷 114-119页

作者：詹巍张军左薇李宁孙志才田禹哈尔滨工业大学市政环境工程学院城市水资源与水环境国家重点实验室黑龙江哈尔滨150090 大庆市自来水有限公司黑龙江大庆163311

随着高分子有机膜材料在水处理领域中应用的不断加深,膜法水处理技术以其优越的性能,成为中国水质高效处理的主导技术。但膜污染却成为阻碍膜法水处理技术发展的关键性问题,如何有效抑制膜污染的发生关系着膜法水处理技术未来发展的前景... 详细信息

随着高分子有机膜材料在水处理领域中应用的不断加深,膜法水处理技术以其优越的性能,成为中国水质高效处理的主导技术。但膜污染却成为阻碍膜法水处理技术发展的关键性问题,如何有效抑制膜污染的发生关系着膜法水处理技术未来发展的前景,目前已成为众多学者开展研究的核心方向。膜改性技术能够赋予现有膜材料新的功能特性,满足膜材料适用于处理各种复杂水质的同时能够抑制膜污染的发生,成为推动膜法水处理发展的前沿方向。膜亲水改性通过将疏水性的膜材料转变为亲水性,提高其抗污染性能。膜光催化改性通过将光催化剂与膜材料相结合,实现膜分离与污染物光催化降解相耦合,可有效抑制膜污染的发生。文章阐述了目前高分子有机膜材料主要的亲水改性技术,并介绍了膜改性新方向-光催化改性的常用方法。

关键词：高分子有机膜膜污染亲水改性光催化改性

基于移动GIS的地下管线三维浏览系统设计与实现

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测绘与空间地理信息 2019年第6期42卷 114-117页

作者：樊静刘康田骊宁中煤航测遥感集团有限公司

随着移动GIS、3DGIS的飞速发展,移动GIS和3DGIS技术的结合使得在移动端上就可以随时随地查看地下管线三维信息,全面掌握其空间分布。笔者从系统需求分析、系统设计、数据组织和功能实现4个方面进行研究,实现了基于移动GIS的地下管线三... 详细信息

随着移动GIS、3DGIS的飞速发展,移动GIS和3DGIS技术的结合使得在移动端上就可以随时随地查看地下管线三维信息,全面掌握其空间分布。笔者从系统需求分析、系统设计、数据组织和功能实现4个方面进行研究,实现了基于移动GIS的地下管线三维浏览系统。该系统实现了地上建筑和地下管线的同时显示,同时支持多种定位方式,能够查询地下管线的属性信息并进行标注,为移动化管理地下管线信息提供了新思路。

关键词：移动GIS 地下管线三维浏览

不同血液净化方式对肝功能衰竭患者微量元素的影响

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临床血液学杂志（输血与检验） 2020年第2期33卷 122-125页

作者：王淑红张陕宁田丽宁宝鸡市康复医院检验科陕西宝鸡721001 宝鸡市第二中医院内科

目的:探讨不同血液净化方式对肝功能衰竭患者微量元素的影响。方法:选取2017-04—2018-05收治的肝功能衰竭患者108例作为研究对象,按就诊顺序单双号分为对照组(54例)和观察组(54例)。对照组患者在内科综合治疗的基础上,予以血浆置换治疗... 详细信息

目的:探讨不同血液净化方式对肝功能衰竭患者微量元素的影响。方法:选取2017-04—2018-05收治的肝功能衰竭患者108例作为研究对象,按就诊顺序单双号分为对照组(54例)和观察组(54例)。对照组患者在内科综合治疗的基础上,予以血浆置换治疗;观察组患者在内科综合治疗的基础上,予以双重血浆分子吸附术治疗。对比患者治疗前后的丙氨酸氨基转移酶(ALT)、凝血酶原时间(PT)、血氨(BA)、铁、镁、锌、铜、硒、白细胞介素-10(IL-10)。结果:2组患者治疗后的ALT、PT、BA、IL-10水平均显著低于治疗前,同时,治疗后观察组患者的ALT、PT、BA、IL-10水平均显著低于对照组;观察组治疗后的铁、镁、锌、硒等微量元素水平显著高于治疗前,且铜元素水平显著低于治疗前;同时,治疗后观察组的铁、镁、锌、硒等微量元素水平显著高于对照组,且铜元素水平显著低于对照组;差异有统计学意义(P<0.05)。结论:相对于血浆置换术,双重血浆分子吸附术能够有效改善患者肝功能,有助于患者体内铜元素的排出,同时有利于镁、铁、锌、硒等微量元素的吸收、储存,从而有效改善患者微量元素的平衡,值得推广应用。

关键词：血浆置换术双重血浆分子吸附术肝功能衰竭微量元素

Polarographic Studies on Associations of Midecamycin and Chloroquine with Hydrogen Peroxide

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Chemical Research in Chinese Universities 2005年第1期21卷 32-35页

作者： lining SONGJun-feng XUMao-tian ZHANGYa InstituteofAnalyticalScience NorthwestUniversityXi＇an710069P.R.China DepartmentofChemistry SuideTeachers′SchoolSuide718000P.R.China

The associations of each of midecamycin and chloroquine phosphate to hydrogen peroxide were studied by using linear-potential scan polarography. In a 0.15 mol/L KH 2PO 4-NaOH(pH 7.4) buffer, the association ratio of the association complex of midecamycin to hydrogen peroxide is 1∶1, and the apparent association constant is 7.18. While in a 0.04 mol/L NH 3·H 2O-NH 4Cl(pH 9.5) buffer, the association ratio and the apparent association constant of the association complex of chloroquine phosphate to hydrogen peroxide are 1∶1 and 45.4, respectively. The formation of the association complexes stabilizes H 2O 2 and results in the accumulation of H 2O 2, which is baneful to human body.

关键词： Midecamycin Chloroquine phosphate Hydrogen peroxide Association Linear-potential scan polarography Reactive oxygen species

温肾通络止痛方通过SENP1-SIRT3通路调控巨噬细胞极化改善骨髓间充质干细胞成骨分化和去睾丸小鼠的骨丢失

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中华中医药杂志 2024年第9期39卷 4619-4626页

作者：李沐哲牛园园张天驰田霖坤孙杰王礼宁郭杨马勇南京中医药大学骨伤修复与重建新技术实验室南京210023 南京中医药大学附属医院南京210029 南京中医药大学无锡附属医院江苏省中医退行性骨关节病临床医学创新中心无锡214071

目的:探讨温肾通络止痛方是否通过巨噬细胞极化促进骨髓间充质干细胞(BMSCs)成骨分化从而发挥抗骨质疏松效应。方法:Micro-CT分析小鼠股骨的骨微结构,HE染色检测小鼠股骨病理变化,免疫组化检测小鼠股骨中OCN、CD86、CD206、SIRT3蛋白表... 详细信息

目的:探讨温肾通络止痛方是否通过巨噬细胞极化促进骨髓间充质干细胞(BMSCs)成骨分化从而发挥抗骨质疏松效应。方法:Micro-CT分析小鼠股骨的骨微结构,HE染色检测小鼠股骨病理变化,免疫组化检测小鼠股骨中OCN、CD86、CD206、SIRT3蛋白表达量,免疫荧光检测小鼠股骨中CD206、F4/80蛋白的表达量。免疫荧光检测RAW264.7细胞iNOS、CD206的荧光强度。qPCR检测RAW264.7细胞CD206、IL-6、iNOS mRNA表达水平。Western Blot检测SENP1、SIRT3蛋白表达量。ALP染色和茜素红染色评估含药血清处理的巨噬细胞条件培养基对BMSCs成骨分化的影响。免疫荧光和Real-time qPCR检测巨噬细胞极化水平。结果:与模型组比较,复方和阿仑膦酸钠处理后BMD、BV/TV增加(P<0.01),OCN阳性细胞数量增加(P<0.05),而脂多糖(LPS)激活M1巨噬细胞后可逆转以上作用(P<0.05,P<0.01)。与对照组比较,模型组CD86阳性细胞数目增加,CD206阳性细胞数目减少(P<0.01);复方和阿仑膦酸钠干预之后,CD86表达减少,CD206表达增加(P<0.01),而LPS干预逆转了复方对巨噬细胞极化的影响(P<0.01)。与模型组比较,含药血清组iNOS、IL-6表达下调(P<0.05,P<0.01),CD206表达上调(P<0.05,P<0.01)。含药血清处理的巨噬细胞条件培养基可增加ALP阳性细胞数、钙化结节数量。复方及其含药血清可促进巨噬细胞SENP1、SIRT3蛋白表达(P<0.05,P<0.01)、小鼠股骨SIRT3的荧光强度(P<0.05)。SENP1抑制剂Momordin Ic可阻断复方对M2标志分子CD206的影响(P<0.05)。结论:温肾通络止痛方可能通过SENP1-SIRT3通路调控巨噬细胞极化改善BMSCs成骨分化从而发挥抗骨质疏松效应。

关键词：骨质疏松症温肾通络止痛方巨噬细胞极化骨髓间充质干细胞 SENP1-SIRT3通路

Inactivity of YGL082W in vitro due to impairment of conformational change in the catalytic center loop

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Science China Chemistry 2020年第2期63卷 237-243页

作者： lining Lu Yu Guo tian Wang Lujun Liang Suwen Zhao Feng Wang Lei Liu Tsinghua-Peking Center for Life Sciences Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology(Ministry of Education)Center for Synthetic and Systems BiologyState Key Laboratory of Chemical Oncogenomics(Shenzhen)Department of ChemistryTsinghua UniversityBeijing 100084China School of Life Science Beijing Institute of TechnologyBeijing 100081China iHuman Institute School of Life Science and TechnologyShanghaiTech UniversityShanghai 201210China

MINDY-1 is a recently discovered new family of deubiquitinating enzymes(DUB),but one of its yeast homologs,YGL082 W,does not show any DUB activity in *** alignment shows that YGL082 W possesses the correct catalytic triad,and yet did not catalyze either the hydrolysis of di-ubiquitin,crosslinking with C-terminally propargylated ubiquitin,or hydrolysis of *** obtaining a crystal structure of the catalytic domain of YGL082 W,we identified an interesting difference between the catalytic center loop of YGL082 W and that of its human homolog *** the conformation of the catalytic center loop was previously reported to be important for the deubiquitination activity of MINDY-1,we hypothesized that Glu27(instead of the corresponding Pro136 in MINDY-1) of the catalytic center loop of YGL082 W may impair the conformational change and account for the lack of *** hypothesis was supported by homology modeling and molecular dynamics simulations,which showed that the Pro-to-Glu mutation(P136 E mutation for MINDY-1) creates a hydrogen bond that inhibits the conformation change of the catalytic center loop of *** experiments through site-directed mutation validated this hypothesis,showing that the P27 E mutation caused MIY1(a homologous active DUB from yeast) to lose activity.

关键词： deubiquitinating enzymes(DUB) enzymology X-ray crystal structure molecular dynamics