检索结果-南通市图书馆

Journal of Environmental Sciences 2012年第12期24卷 2141-2148页

作者： Jing Ma Li Xu lingyun jia School of Life Science and Biotechnology Dalian University of TechnologyLiaoning 116023China

It is important to screen strains that can decompose polycyclic aromatic hydrocarbons （PAHs） completely and rapidly with good adaptability for bioremedlation in a local area. A bacterial strain JM2, which uses pbenanthrene as its sole carbon source, was isolated from the active sewage sludge from a chemical plant in Jilin, China and identified as Pseudomonas based on 16S rDNA gene sequence analysis. Although the optimal growth conditions were determined to be pH 6.0 and 37~C, JM2 showed a broad pH and temperature profile. At pH 4.5 and 9.3, JM2 could degrade more than 40% of fluorene and phenanthrene （50 mg/L each） within 4 days. In addition, when the temperature was as low as 4~C, JM2 could degrade up to 24% fluorene and 12% phenanthrene. This showed the potential for JM2 to be applied in bioremediation over winter or in cold regions. Moreover, a nutrient augmentation study showed that adding formate into media could promote PAH degradation, while the supplement of salicylate had an inhibitive effect. Furthermore, in a metabolic pathway study, salicylate, phthalic acid, and 9-fluorenone were detected during the degradation of fluorene or phenanthrene. In conclusion, Pseudomonas sp. JM2 is a high performance strain in the degradation of fluorene and phenanthrene under extreme pH and temperature conditions. It might be useful in the bioremediation of PAHs.

关键词： polycyclic aromatic hydrocarbons Pseudomonas sp. biodegradation formate salicylate

Cell viability in the cadmium-stressed cell suspension cultures of tobacco is regulated by extracellular ATP,possibly by a reactive oxygen species-associated mechanism

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2020年第1期44卷 89-99页

作者： Zhenzhen SHI jiaxin CAO Hailong PANG lingyun jia Kun SUN Hanqing FENG College of Life Science Northwest Normal UniversityLanzhou730070China

Cadmium(Cd)is one of the most widespread and toxic heavy metals to *** ATP(exATP)is thought to be an extracellular effector in regulating the physiological responses of plant cells to environmental ***,the function of exATP in Cd-stressed plant cells is much *** present work showed that treating tobacco(Nicotiana tabacum *** Yellow-2)cell-suspension cultures with exogenous CdCl2 reduced the cell viability,exATP level,and Mg ***,the production of reactive oxygen species(ROS),Cd content,and electrolyte leakage of the cells were enhanced by exogenous *** the Cd-induced accumulation of ROS was decreased by the supplement with DMTU(dimethylthiourea,a scavenger of ROS),the Cd-induced increases of the electrolyte leakage and Cd content were alleviated,and the Cd-induced reductions of cell viability were partly rescued,suggesting that Cd-induced reduction of cell viability could be related to the ROS *** the condition of Cd stress,when the reduction of exATP level was partly rescued by exogenous ATP(20μM),the increases of ROS production,electrolyte leakage,and Cd content were attenuated,and the reduction of cell viability was also *** observations indicate that exATP can regulate the cell viability in the Cd–stressed plant cells possibly by an ROS-associated mechanism.

关键词： Cell viability Cd ions Extracellular ATP Reactive oxygen species

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

亲和色谱仿生配基的筛选和设计

分析化学 2005年第9期33卷 1345-1349页

作者：任军贾凌云大连理工大学环境与生命学院生物科学与工程系大连理工大学环境与生命学院生物科学与工程系大连116023

利用选择性好、稳定性高、价格低廉的仿生配基作为亲和色谱的功能基团,实现对目的蛋白质的亲和分离,可以弥补常规的单克隆抗体和其他天然蛋白质配基在价格、稳定性方面的不足,从而拓展亲和色谱技术的应用范围。本文综述了亲和色谱仿生... 详细信息

利用选择性好、稳定性高、价格低廉的仿生配基作为亲和色谱的功能基团,实现对目的蛋白质的亲和分离,可以弥补常规的单克隆抗体和其他天然蛋白质配基在价格、稳定性方面的不足,从而拓展亲和色谱技术的应用范围。本文综述了亲和色谱仿生配基在筛选和设计方面的研究进展,重点介绍了组合方法和基于结构的仿生配基的设计及其应用。

关键词：亲和色谱仿生配基组合化学合理性配基设计

Activity of xanthones isolated from Lomatogonium rotatum

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Traditional Medicines 2020年第3期15卷 113-120页

作者： Qingdi Mu Qin He Mengyuan Tan Ruoning Wang Jing Wang Chongning Lv Hang He Jincai Lu lingyun jia School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityBox 79Shenyang 110016Liaoning ProvincePR China

Thirteen xanthones were isolated from ***,which were established as l,8-dihydroxy-3,4,5-trimethoxyxanthone(1);l-hydroxy-3异,8-trimethoxyxanthone(2);8-hydroxy-l,3?5-trimethoxyxaiithone(3);l-hydroxy-3,4,5-trimethoxyxanthone(4);l-hydroxy-3,5,8-trimethoxyxanthone(5);l,4,8-trihydroxy-5,6-dimethoxyxanthone(6);l,3,8-trihydroxy-4,7-dimethoxyxanthone(;7)l,8-dihydroxy-3,7-dimethoxyxanthone(8);l-hydroxy-3,5-dimethoxyxanthone(9);l-hydroxy-2,3,4,5-tetramethoxyxanthone(;10)l-hydroxy-2,3,4,7-tetramethoxyxanthone(11);l,7-dihydroxy-3,8-dimethoxyxanthone(12);rel-l,3,5,8-tetrahydroxy-2,4,6,7-tetrahydroxanthen-9-one(13).Compounds 4,6,7,9,10,11,12 and 13 are reported for the first time from ***,the activity of xanthones was assessed.

关键词： Lomatogonium rotatum,xanthones activity

Antimicrobial activities and analysis of the crude extract,fractions and compounds from the Lomatogonium rotatum

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Traditional Medicines 2020年第4期15卷 145-152页

作者： Ruoning Wang Qin He Qingdi Mu Mengyuan Tan Jing Wang Chongning Lv Hang He Jincai Lu lingyun jia School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityBox 79Shenyang 110016Liaoning ProvincePR China

This study was evaluated the antimicrobial activities of the crude extract,different fractions,and compounds from the plant of Lomatogonium *** fractions of chloroform and compounds 5,2 and 6 exhibited stronger antimicrobial *** 5 was the most active while Staphylococcus aureus and Candida albicans were sensitive to all the tested *** existence of a relationship between the xanthone structures and inhibition of microorganism *** antimicrobial activity of this plant was reported here *** results provided evidence that the studied plant extract,fractions as well as some of the isolated compounds might be potential sources of new antimicrobial drug.

关键词： Lom atogonium carinthiacum fractions compounds antimicrobial activities HPLC-EIS-MS/MS

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

外商投资企业是否有更高的退出风险

世界经济 2017年第7期40卷 52-77页

作者：高凌云屈小博贾朋中国社会科学院世界经济与政治研究所中国社会科学院人口与劳动经济研究所

本文基于1998-2008年中国工业企业数据,在严格界定企业退出的前提下,利用能控制多重生存期和不可观测异质性的离散时间生存分析方法,检验了中国以不同方式定义的外商投资企业是否存在"自由流动"特性。核心结论有:中国外商投资企业的无... 详细信息

本文基于1998-2008年中国工业企业数据,在严格界定企业退出的前提下,利用能控制多重生存期和不可观测异质性的离散时间生存分析方法,检验了中国以不同方式定义的外商投资企业是否存在"自由流动"特性。核心结论有:中国外商投资企业的无条件和条件退出风险均低于内资企业;这一所有权溢价主要源于"干中学"和合资、合作方的"相互学"。另外,企业和产业层面的特征也会对外商投资企业退出风险产生显著影响。上述结论从退出风险的角度为中国坚持扩大外资准入、放松外资股权比例限制等政策提供了经验支撑。

关键词：外资溢价自由流动生存分析

Analysis of the chemical composition of Angelicae Pubescentis Radix by ultra-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry

在线全文

学校读者我要写书评

暂无评论

Journal of Chinese Pharmaceutical Sciences 2019年第3期28卷 145-159页

作者： Meiqi Wan Youbo Zhangl Yanfang Yang Xiaoyan Liu lingyun jia Xiuwei Yang Slate Key Laboratory of Natural and Biomimetic Drugs Department of Natural Medicines School of Pharmaceutical SciencesPeking University Health Science Center Beijing 100191 China A school of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China

Angelicae Pubescentis Radix (APR, Duhuo) is a commonly used traditional Chinese medicine and usually used for the treatments of rheumatic diseases. To assess the chemical composition of APR extract, a sensitive and reliable UPLC-Q?TOF-MS method was used for qualitative analysis. The separation was achieved on an Agilent SB-Ci8 column (1.8 pm, 2.1 mm><50 mm) with a gradient elution system consisting of acetonitrile and water containing 0.1% formic acid. An electrospray ionization (ESI) was used for mass spectrometer, and the data were collected in the positive ion mode, which was operated in a full-scan mode at m/z 100-800. A total of 49 compounds including 46 coumarins were identified according to the MS and MS/MS data. Among them, two were new compounds, and isoangenomalin, scoparone, 4-methyl-umbelliferyl acetate, suberenol,/raw5-dehydroosthol, and oroselone were first reported in APR.

关键词： Angelicae Pubescentis Radix UPLC/Q-TOF-MS Chemical composition

胞外ATP参与调控拟南芥对过量强度光照的部分生理学响应

在线全文

学校读者我要写书评

暂无评论

植物生理学报 2022年第8期58卷 1528-1536页

作者：王馨李丽李颖贾凌云冯汉青西北师范大学生命科学学院兰州730070

植物在自然环境中往往受到过量强度光照的影响。作为植物中较新鉴定的信号分子,胞外ATP (extracellular ATP,eATP)是否参与了植物对过量强度光照的生理学响应尚不清楚。本研究发现,过量强度光照使得拟南芥(Arabidopsis thaliana)植株叶... 详细信息

植物在自然环境中往往受到过量强度光照的影响。作为植物中较新鉴定的信号分子,胞外ATP (extracellular ATP,eATP)是否参与了植物对过量强度光照的生理学响应尚不清楚。本研究发现,过量强度光照使得拟南芥(Arabidopsis thaliana)植株叶片胞外ATP水平上升、光系统II (photosystem II,PSII)受到抑制、气孔开度下降,而过氧化氢(HO)含量、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性升高。通过野生型(Col)和植物胞外ATP受体突变型(dorn1-3)拟南芥对比发现,在过量强度光照下2种植株叶片PSII均受到损伤,但较之野生型植株,dorn1-3植株受损伤程度较低。过量强度光照引起了野生型植株叶片中APX和POD活性的升高,但并未引起dorn1-3植株内2种酶活性显著性变化。同时,在过量强度光照下dorn1-3植株叶片气孔开度显著高于野生型,但过量强度光照下dorn1-3植株叶片H_(2)O_(2)含量和野生型相比无显著差异。因此,上述结果表明,在过量强度光照下,拟南芥eATP水平的上升通过刺激其受体DORN,参与了植物PSII的抑制和气孔的关闭,并同时调节了抗氧化酶APX和POD的活性。

关键词：过量强度光照胞外ATP 叶绿素荧光 H2O2 抗氧化酶

在线全文

学校读者我要写书评

暂无评论

智慧教育背景下大学英语听说教学设计

安徽工业大学学报（社会科学版） 2023年第5期40卷 56-58,66页

作者：贾玲云安徽工业大学外国语学院安徽马鞍山243002

以有效教学理论为指导,将BOPPPS模型与超星平台教学工具相结合,构建了基于BOPPPS模型结合超星平台的大学英语听说教学模式,并以“大学英语”课程为依托,进行了教学创新与实践。结果显示,新教学模式可以有效提高学生的英语听说水平。

关键词： BOPPPS模型超星大学英语听说教学设计

在线全文

学校读者我要写书评

暂无评论

微生物降解重油的初步研究

化工环保 2005年第6期25卷 427-430页

作者：史继诚贾凌云大连理工大学生物科学与工程系辽宁大连116024

从石油污染的土壤中筛选出一株假单胞菌,考察了其对重油的降解效果。降解18d,重油的降解率达到42.8%,饱和烃、芳香烃、胶质、沥青质的降解率分别为42.95%,43.85%,44.5%,5.55%;质量浓度2.5g/L的表面活性剂Tw een-80可使重油的18d降解率达... 详细信息

从石油污染的土壤中筛选出一株假单胞菌,考察了其对重油的降解效果。降解18d,重油的降解率达到42.8%,饱和烃、芳香烃、胶质、沥青质的降解率分别为42.95%,43.85%,44.5%,5.55%;质量浓度2.5g/L的表面活性剂Tw een-80可使重油的18d降解率达到51.0%;弱碱性条件有利于重油的生物降解;生物泥浆法处理重油污染的土壤,1kg土壤中重油质量100g,降解45d,重油的降解率达到38.85%。

关键词：微生物降解重油土壤污染