检索结果-南通市图书馆

Soybean AP1 homologs control flowering time and plant height

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2020年第12期62卷 1868-1879页

作者： Liyu Chen Haiyang Nan lingping kong Lin Yue Hui Yang Qingsong Zhao Chao Fang Haiyang Li Qun Cheng Sijia Lu Fanjiang kong Baohui Liu Lidong Dong Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China The Key Laboratory of Crop Genetics and Breeding of Hebei Institute of Cereal and Oil CropsHebei Academy of Agricultural and ForestrySciencesShijiazhuang 050000China National Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern CropProductionNanjing Agricultural UniversityNanjing 210000China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography andAgroecologythe Chinese Academy of SciencesHarbin 150000China

Flowering time and plant height are key agronomic traits that directly affect soybean(Glycine max)***1(AP1)functions as a class A gene in the ABCE model for floral organ development,helping to specify carpel,stamen,petal,and sepal *** are four AP1 homologs in soybean,all of which are mainly expressed in the shoot ***,we used clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1,with loss-of-function mutations in all four GmAP1 *** short-day(SD)conditions,the gmap1 quadruple mutant exhibited delayed flowering,changes in flower morphology,and increased node number and internode length,resulting in plants that were taller than the wild ***,overexpression of GmAP1a resulted in early flowering and reduced plant height compared to the wild type under SD *** gmap1 mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism,thereby providing insight into the role of GmAP1 in the regulatory networks controlling flowering time and plant height in *** node number is the trait with the most promise for enhancing soybean pod number and grain ***,the mutant alleles of the four AP1 homologs described here will be invaluable for molecular breeding of improved soybean yield.

关键词： soybean breeding height

Vivid,Concise and Marvelous Paper Cutting--Exhibition of Paper-cutting Artwsorks by Zhang Yongzhong

在线全文

学校读者我要写书评

暂无评论

China & The World Cultural Exchange 2023年第5期 33-35页

作者： kong lingping China National Arts and Crafts Museum China Intangible Cultural Heritage Museum

Paper-cutting is a more common form of folk art expression in Shanxi *** often use scissors or carving knives to hollow out and cut patterns on the paper to decorate their own good *** Yongzhong's paper-cutting inherits the artistic creation techniques of the Northern School of paper-cutting,mostly using the theme of sacrifice to express the belief in harmonious coexistence between heaven and *** works not only reflect the creation of folk life,but also promote the storyline of traditional Shanxi *** artistic creation style is unique.

关键词： Province harmonious mostly

A recent retrotransposon insertion of J caused E6 locus facilitating soybean adaptation into low latitude

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2021年第6期63卷 995-1003页

作者： Chao Fang Jun Liu Ting Zhang Tong Su Shichen Li Qun Cheng lingping kong Xiaoming Li Tiantian Bu Haiyang Li Lidong Dong Sijia Lu Fanjiang kong Baohui Liu Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and Agroecologythe Chinese Academy of SciencesHarbin 150000China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical Gardenthe Chinese Academy of SciencesGuangzhou 510650China National Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China

Soybean(Glycine max) is an important legume crop that was domesticated in temperate *** varieties from these regions generally mature early and exhibit extremely low yield when grown under inductive short-day(SD) conditions at low latitudes. The long-juvenile(LJ) trait, which is characterized by delayed flowering and maturity,and improved yield under SD conditions, allowed the cultivation of soybean to expand to lower latitudes. Two major loci control the LJ trait: J and E6. In the current study, positional cloning, sequence analysis, and transgenic complementation confirmed that E6 is a novel allele of J, the ortholog of Arabidopsis thaliana EARLY FLOWERING 3(ELF3). The mutant allele e6^(PG), which carries a Ty1/Copia-like retrotransposon insertion, does not suppress the legume-specific flowering repressor E1, allowing E1 to inhibit Flowering Locus T(FT) expression and thus delaying flowering and increasing yields under SD conditions. The e6^(PG)allele is a rare allele that has not been incorporated into modern breeding *** dysfunction of J might have greatly facilitated the adaptation of soybean to low latitudes. Our findings increase our understanding of the molecular mechanisms underlying the LJ trait and provide valuable resources for soybean breeding.

关键词： E6 long-juvenile(LJ)trait J Ty1/copia-like retrotransposon

汉麻纤维TEMPO、4OH-TEMPO氧化作用对比分析

在线全文

学校读者我要写书评

暂无评论

上海纺织科技 2025年第1期53卷 11-14,47页

作者：杜霈宇孔令苹裴笠舟李松林常换达齐齐哈尔大学轻工与纺织学院黑龙江齐齐哈尔161006 齐齐哈尔生态环境监测中心黑龙江齐齐哈尔161000

为优选汉麻纤维联合脱胶方法中的氧化剂,采用TEMPO和4OH-TEMPO两种氧化剂分别对汉麻纤维进行氧化处理,评估处理前后汉麻纤维的失重率、分裂度、官能团、结晶度、形态结构和力学性能等理化性能差异。研究结果表明,4OH-TEMPO和TEMPO氧化后... 详细信息

为优选汉麻纤维联合脱胶方法中的氧化剂,采用TEMPO和4OH-TEMPO两种氧化剂分别对汉麻纤维进行氧化处理,评估处理前后汉麻纤维的失重率、分裂度、官能团、结晶度、形态结构和力学性能等理化性能差异。研究结果表明,4OH-TEMPO和TEMPO氧化后,汉麻纤维失重率分别为8.7%和5.7%,纤维强力损失率分别为29.5%和22.35%。结合FTIR图和XRD图发现,氧化后,汉麻纤维的晶型不变,强力损失差别不大。4OH-TEMPO和TEMPO氧化后汉麻纤维分裂度分别为166.41和141.17,未脱胶汉麻纤维分裂度为62.95。结合SEM图对比发现,4OH-TEMPO对汉麻纤维的氧化处理效果在纤维细度、手感和胶质去除程度方面优于TEMPO。

关键词： TEMPO 4OH-TEMPO 氧化汉麻纤维性能

Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第10期64卷 1866-1882页

作者： Lidong Dong Zhihong Hou Haiyang Li Zhaobo Li Chao Fang lingping kong Yongli Li Hao Du Tai Li Lingshuang Wang Milan He Xiaohui Zhao Qun Cheng Fanjiang kong Baohui Liu Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou Higher Education Mega Center230 Waihuanxi RoadGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqingChina National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China College of Agriculture Jilin Agricultural Science and Technology UniversityJilin 132101China

Salt stress and flowering time are major factors limiting geographic adaptation and yield productivity in soybean(Glycine max).Although improving crop salt tolerance and latitude adaptation are essential for efficient agricultural production,whether and how these two traits are integrated remains largely ***,we used a genome-wide association study to identify a major salt-tolerance locus controlled by E2,an ortholog of Arabidopsis thaliana GIGANTEA(GI).Loss of E2 function not only shortened flowering time and maturity,but also enhanced salt-tolerance in soybean.E2 delayed soybean flowering by enhancing the transcription of the core flowering suppressor gene E1,thereby repressing Flowering Locus T(FT)*** E2 knockout mutant e2^(CR) displayed reduced accumulation of reactive oxygen species(ROS)during the response to salt stress by releasing peroxidase,which functions in ROS scavenging to avoid *** and population genetic analyses also suggested that loss-of-function e2 alleles have been artificially selected during breeding for soybean adaptation to high-latitude regions with greater salt *** findings provide insights into the coupled selection for adaptation to both latitude and salt stress in soybean;and offer an ideal target for molecular breeding of early-maturing and salt-tolerant cultivars.

关键词： floweringtime GIGANTEA ROS saltstress soybean

Neuroprotective effects of human telomerase reverse transcriptase on beta-amyloid fragment 25-35-treated human embryonic cortical neurons

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2009年第6期4卷 405-412页

作者： lingping kong Lingzhi Wu Jie Zhang Yaping Liao Huaqiao Wang Department of Anatomy and Brain Research Zhongshan School of Medicine Sun Yat-sen University Guangzhou 510080 Guangdong Province China Meizhou People's Hospital Meizhou 514031 Guangdong Province China Department of Anatomy Preclinical College Guangdong University of Pharmacy Guangzhou 510006 Guangdong Province China Department of Bioscience Bengbu Medical College Bengbu 233030 Anhui Province China

BACKGROUND： Numerous current studies have suggested that human telomerase reverse transcriptase （hTERT） gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses; however, the mechanism of action remains unknown. OBJECTIVE： To evaluate the neuroprotective effects and possible mechanism of action of hTERT gene transfection in human embryonic cortical neurons treated with beta-amyloid fragment 25-35 （AI325-35）. DESIGN, TIME AND SETTING： The randomized, controlled and molecular biological studies were performed at the Department of Anatomy and Brain Research, Zhongshan School of Medicine, Sun Yat-sen University, China, from September 2005 to June 2008. MATERIALS： AdEasy-1 Expression System was gifted by Professor Guoquan Gao from Sun Yat-Sen University, China. Human cortical neurons were derived from 12-20 week old aborted fetuses, obtained from the Guangzhou Maternal and Child Health Hospital, China. Mouse anti-Odk5 and mouse anti-p16 monoclonal antibodies （Lab Vision, USA）, and mouse anti-hTERT monoclonal antibody （Epitomics, USA）, were used in this study. METHODS：（1） Recombinant adenovirus vectors, encoding hTERT （Ad-hTERT） and green fluorescent protein （Ad-GFP）, were constructed using the AdEasy-1 Expression System. Human embryonic cortical neurons in the Ad-hTERT group were transfected with Ad-hTERT for 1-21 days. Likewise, human embryonic cortical neurons in the Ad-GFP group were transfected with Ad-GFP for 1-21 days. Human embryonic cortical neurons in the control group were cultured as normal. （2） Human embryonic cortical neurons in the Ad-hTERT group were treated with 10 pmol/L Aβ25-35 for 24 hours. Normal human embryonic cortical neurons treated with 10 pmol/Lβ25.35 for 24 hours served as a model group. Human embryonic cortical neurons in the Ad-GFP and control groups were not treated with Aβ25-35. MAIN OUTCOME MEASURES： Expression of hTERT in human embryonic cortical neurons was evaluated by immunocytochemical staining

关键词： human telomerase reverse transcriptase cortical neuron human embryo Alzheimer's disease beta-amyloid fragment 25-35 Cdk5 p16

Dt1 inhibits SWEET-mediated sucrose transport to regulate photoperiod-dependent seed weight in soybean

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2024年第3期17卷 496-508页

作者： Xiaoming Li Zhonghui Chen Haiyang Li Lin Yue Cuirong Tan Hongjie Liu Yilong Hu Yuhua Yang Xiani Yao lingping kong Xiang Huangi Bin Yu Chunyu Zhang Yuefeng Guan Baohui Liu Fanjiang kong Xingliang Hou Guangdong Provincial Key Laboratory of Applied Botany&State Key Laboratory of Plant Diversity and Specialty Crops South China Botanical GardenChinese Academyof SciencesGuangzhou510650China Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China University of Chinese Academy of Sciences Beijing 100049China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length *** weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly *** this study,we identify SwW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 *** showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in *** analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long ***,Dt1 does not function in seed development under short days due to its very low ***,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of ***,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weightandgrowth habit.

关键词： soybean seed weight photoperiod dt1 sucrose transport

Development and validation of InDel markers for identification of QTL underlying flowering time in soybean

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2018年第2期6卷 126-135页

作者： Jialin Wang lingping kong Kanchao Yu Fengge Zhang Xinyi Shi Yanping Wang Haiyang Nan Xiaohui Zhao Sijia Lu Dong Cao Xiaoming Li Chao Fang Feifei Wang Tong Su Shichen Li Xiaohui Yuan Baohui Liu Fanjiang kong The Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina School of Life Sciences Guangzhou UniversityGuangzhou 510006GuangdongChina Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences Qiqihar 161006HeilongjiangChina Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157041HeilongjiangChina University of Chinese Academy of Sciences Beijing 100049China College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina The Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina.

Soybean [Glycine max(L.) Merrill] is a major plant source of protein and oil. An accurate and well-saturated molecular linkage map is a prerequisite for forward genetic studies of gene function and for modern breeding for many useful agronomic traits. Next-generation sequence data available in public databases provides valuable information and offers new insights for rapid and efficient development of molecular markers. In this study, we attempted to show the feasibility and facility of using genomic resequencing data as raw material for identifying putative In Del markers. First, we identified 17,613 In Del sites among 56 soybean accessions and obtained 12,619 primer pairs. Second, we constructed a genetic map with a random subset of 2841 primer pairs and aligned 300 polymorphic markers with the 20 consensus linkage groups(LG). The total genetic distance was 2347.3 c M and the number of mapped markers per LG ranged from 10 to 23 with an average of 15 markers. The largest and smallest genetic distances between adjacent markers were 52.3 c M and 0.1 cM, respectively. Finally, we validated the genetic map constructed by newly developed In Del markers by QTL analysis of days to flowering(DTF) under different environments. One major QTL(qDTF4) and four minor QTL(qDTF20, qDTF13, qDTF12,and q DTF11) on 5 LGs were detected. These results demonstrate the utility of the In Del markers developed in this work for map-based cloning and molecular breeding in soybean.

关键词： Soybean Resequencing data InDel markers Genetic map QTL analysis

在线全文

学校读者我要写书评

暂无评论

中国电信管理网的建设及展望

电信科学 1998年第7期14卷 17-22页

作者：孔令萍中国邮电电信总局

本文论述了建设电信管理网的意义和目的，比较全面地介绍了我国电信网络管理系统的开发、建设和应用的基本情况，对电信网络管理系统的实现技术做了简要的展望。

关键词：电信网电信管理网建设中国