检索结果-南通市图书馆

The role of SLC12A family of cation-chloride cotransporters and drug discovery methodologies

Journal of Pharmaceutical Analysis 2023年第12期13卷 1471-1495页

作者： Shiyao Zhang Nur Farah Meor Azlan Sunday Solomon Josiah Jing Zhou Xiaoxia Zhou lingjun jie Yanhui Zhang Cuilian Dai Dong Liang Peifeng Li Zhengqiu Li Zhen Wang Yun Wang Ke Ding Yan Wang Jinwei Zhang Xiamen Cardiovascular Hospital of Xiamen University School of MedicineXiamen UniversityXiamenFujian363001China Institute of Biomedical and Clinical Sciences Medical SchoolFaculty of Health and Life SciencesUniversity of ExeterExeterEX44PSUK Department of Neurology Institutes of Brain ScienceState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain ScienceInstitute of Biological ScienceZhongshan HospitalFudan UniversityShanghai200032China Aurora Discovery Inc. FoshanGuangdong528300China Institute for Translational Medicine Qingdao UniversityQingdaoShandong266021China School of Pharmacy Jinan UniversityGuangzhou510632China State Key Laboratory of Chemical Biology Research Center of Chemical KinomicsShanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032China

The solute carrier family 12(SLC12)of cation-chloride cotransporters(CCCs)comprises potassium chloride cotransporters(KCCs,***1,KCC2,KCC3,and KCC4)-mediated Cl^(-)extrusion,and sodium potassium chloride cotransporters(N[K]CCs,NKCC1,NKCC2,and NCC)-mediated Cl^(-)*** CCCs play vital roles in cell volume regulation and ion ***-of-function or loss-of-function of these ion transporters can cause diseases in many *** recent years,there have been considerable advances in our understanding of CCCs'control mechanisms in cell volume regulations,with many techniques developed in studying the functions and activities of *** approaches to directly measure CCC activity involve assays that measure the transport of potassium substitutes through the *** techniques include the ammonium pulse technique,radioactive or nonradioactive rubidium ion uptakeassay,and thallium ion-uptake ***'activity can also be indirectly observed by measuring gaminobutyric acid(GABA)activity with patch-clamp electrophysiology and intracellular chloride concentration with sensitive microelectrodes,radiotracer^(36)Cl^(-),and fluorescent *** techniques include directly looking at kinase regulatory sites phosphorylation,flame photometry,22Nat uptake assay,structural biology,molecular modeling,and high-throughput drug *** review summarizes the role of CCCs in genetic disorders and cell volume regulation,current methods applied in studying CCCs biology,and compounds developed that directly or indirectly target the CCCs for disease treatments.

关键词： Cation-chloride cotransporters Chloride volume regulation Cotransporter assays Drug discovery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

施用锌肥和硼肥对玉米穗粒性状和品质的影响

引用

核农学报 2017年第2期31卷 371-378页

作者：张勇强宋航薛志伟牛平平介领军李潮海河南农业大学农学院河南郑州450002 安阳市农业科学院河南安阳455000 鄢陵县农业局河南许昌461200 郑州市农业技术推广中心河南郑州450002 河南粮食作物协同创新中心河南郑州450002

为了探究微量元素锌和硼对玉米果穗的影响,以豫单606为材料,采用盆栽试验,设置不施用锌肥和硼肥(对照,CK)、施用锌肥、施用硼肥、同时施用锌肥和硼肥4种处理,调查了玉米穗粒性状和品质的变化。结果表明,施用锌肥和硼肥,秃尖减少,行粒数... 详细信息

为了探究微量元素锌和硼对玉米果穗的影响,以豫单606为材料,采用盆栽试验,设置不施用锌肥和硼肥(对照,CK)、施用锌肥、施用硼肥、同时施用锌肥和硼肥4种处理,调查了玉米穗粒性状和品质的变化。结果表明,施用锌肥和硼肥,秃尖减少,行粒数增加,产量显著提高;施用锌肥籽粒长度增加,粒厚、百粒体积和百粒重降低,由于穗粒数提高,单穗产量增加;施用硼肥籽粒长度、百粒重、百粒体积和单穗产量均增加。与CK相比,单施锌肥、硼肥及锌肥和硼肥配施均能提高籽粒蛋白质含量;施用锌肥籽粒脂肪含量显著提高,施用硼肥籽粒脂肪含量降低;各施肥处理淀粉含量均无显著差异。与CK相比,施用锌肥或硼肥以及锌肥和硼肥配施显著提高了籽粒锌和硼的含量。本研究结果为锌肥和硼肥在玉米生产中的应用提供了参考。

关键词：锌肥硼肥玉米穗粒性状品质

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Constructing bifunctional Co/MoC@N-C catalyst via an in-situ encapsulation strategy for efficient oxygen electrocatalysis

引用

Journal of Energy Chemistry 2021年第8期30卷 538-546,I0011页

作者： Hui Huang lingjun Kong Ming Liu jie He Wei Shuang Yunhua Xu Xian-He Bu School of Materials Science and Engineering National Institute for Advanced MaterialsTKL of Metal and Molecule Based Material ChemistryNankai University300071 TianjinChina School of Materials Science and Engineering Key Laboratory of Advanced Ceramics and Machining Technology(Ministry of Education)and Tianjin Key Laboratory of Composite and Functional MaterialsTianjin University300072 TianjinChina Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai University300071 TianjinChina

The large-scale application of Zn-air battery requires the development of efficient,low-cost,and stable bifunctional electrocatalysts for the oxygen evolution reaction(OER)and oxygen reduction reaction(ORR).Herein,an electrocatalyst of a Co/Mo C-nanoparticles embedded N-doped carbon(Co/Mo C@N-C)was prepared via the in-situ encapsulation of Co_(2)(CO)_(8)and Mo(CO)6 in ZIF-8 using ball-milling,followed by pyrolysis under an Ar *** used as a bifunctional electrocatalyst,the as-prepared Co/Mo C@N-C showed an ORR half-wave potential of 0.824 V in an alkaline medium and an overpotential of only 290 m V for the OER at 10 m A cm^(-2),which was 70 m V lower than Ru O_(2).The Co/Mo C@N-C was also evaluated as a cathode in a solid-state Zn-air battery and delivered a higher peak discharge power density and better cycling stability(over 1600 min)than Pt/C-Ru O_(2).Most importantly,our work provides a new strategy to prepare bifunctional catalysts and promotes their applications in the energy conversion field.

关键词： In-situ ZIF-8 Bifunctional electrocatalyst New strategy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deciphering of advantageous electrocatalytic water oxidation behavior of metal-organic framework in alkaline media

引用

Nano Research 2021年第12期14卷 4680-4688页

作者： Ming Liu lingjun Kong Xuemin Wang jie He Jijie Zhang Jian Zhu Xian-He Bu School of Materials Science and Engineering National Institute for Advanced MaterialsTKL of Metal and Molecule Based Material ChemistryNankai UniversityTianjin300350China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin300071China

Carboxylic acid-based metal-organic frameworks(MOFs)are normally passed for the“pre-catalysts”for oxygen evolution reaction(OER)due to the hydroxides constructed in-situ during its alkaline hydrolysis process(AHP)in ***,it remains a mystery that they show advantageous activity over prototypical hydroxides when they are directly acted as OER ***,we propose for the first time that the steric hindrance effect of Nafion can induce enhanced catalytic activity of such *** from conventional catalysts without AHP,the Nafion with 3D structure weakens the AHP of Co-MOF nanoribbons,thus forming small size and low crystallinity species(cobalt hydroxide)with more active *** the existence of Nafion also optimizes its electronic structure,which is confirmed by transmission electron microscopy(TEM),in-situ UV absorption spectra,in-situ Raman spectroscopy and so *** with Co-MOF-K obtained by directly immersing the Co-MOF nanoribbons in 1.0 M KOH,the Co-MOF-NK obtained by AHP of Co-MOF mixed with Nafion shows better catalytic *** on the above inspiration,we realized the low overpotential of 268 mV at 10 mA·cm^(−2) by preparing *** work provides a new understanding of the structural reconstruction of MOFs in the field of electrocatalysis.

关键词： metal-organic frameworks structural reconstruction Nafion stereo hindrance water oxidation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Factors influencing the degree of participation in surgical decision-making among Chinese patients with prostate cancer:A qualitative research

引用

Asian Journal of Urology 2022年第2期9卷 177-185页

作者： jie Cao Chunxue Peng Xiaoying Lu lingjun Zhou Jing Wu Department of Urology Changhai HospitalNaval Medical UniversityShanghaiChina Department of Nursing Changhai HospitalNaval Medical UniversityShanghaiChina School of Nursing Naval Medical UniversityShanghaiChina

Objective:There was increasingly demand of participation in surgical decision-making among Chinese patients with prostate ***,due to the complex healthcare system and advanced care settings,it is quite challenging for the patients to gain sufficient support from the institute and the *** research aimed to investigate the factors that impact the degree of participation in surgical decision-making among Chinese prostate cancer ***:A phenomenological approach of qualitative research based on the results of semi-structured interviews was adopted,to explore the influencing factors which hinder the participation in surgical *** Criteria for Reporting Qualitative Research were *** to 160 post-operative patients who had undergone radical prostatectomy along with 68 medical and nursing staffs,were purposively recruited in this *** retrospective study was carried out from September 2018 to August *** recording and transcribing the interviews,the interview materials were evaluated via the Colaizzi's seven step approach and the NVivo Version 10 software to analyze the interview ***:According to the analysis and summary of the interviews,there were three factors affecting the degree of participation in surgical ***,insufficient information was provided by medical and nursing staffs because of their lack of time,proper communication skills,and career experience,as well as difficulties in the development of patient decision aid and inconsistent resource ***,the cognitive level of decision-making among patients was relatively low due to poor psychological endurance,insufficient amount of education,senility,and less knowledge and information ***,decisions were constantly made by family members with/without ***:The degree of participation of Chinese prostate cancer patients in the surgical decision-making had much space

关键词： Prostate cancer Participation Decision-making Surgery Influence factors Qualitative research

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Controlled synthesis of core-shell Fe_(2)O_(3)@N-C with ultralong cycle life for lithium-ion batteries

引用

Chinese Chemical Letters 2022年第2期33卷 1037-1041页

作者： Hui Huang lingjun Kong Wei Shuang Wei Xu jie He Xian-He Bu School of Materials Science and Engineering TKL of Metal and Molecule Based Material ChemistryNankai UniversityTianjin 300350China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin 300071China

Development of low-cost electrode materials with long cycle life and high volumetric capacity is im-portant for large-scale applications of lithium-ion batteries(LIBs).Here,an electrode made from Fe_(2)O_(3) encapsulated with N-doped carbon(Fe_(2)O_(3)@N-C)via ZIF-8 coating and carbonization process is reported.A cavity was generated between the Fe_(2)O_(3)and N-C material during the carbonization process that is conducive to alleviating the volume expansion of Fe_(2)O_(3).As a result,the Fe_(2)O_(3)@N-C composite exhibits a high specific capacity(1064 mAh/g at 0.1 A/g)and cycle stability(803.6 mAh/g at 1.0 A/g after 1100 cycles)when used as the LIB *** addition,the influence of carbonization under air on the LIB per-formance was investigated by controllably changing the crystal phase of Fe_(2)O_(3)and the thickness of the carbon *** work provides a new method for the design and fabrication of yolk-shell composite electrodes for LIBs and other applications.

关键词： Lithium-ion batteries Anode Core-shell structure Fe_(2)O_(3) N-doped carbon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Production of transgenic mice by random recombination of targeted genes in female germline stem cells

引用

Journal of Molecular Cell Biology 2011年第2期3卷 132-141页

作者： Yong Zhang Zhaojuan Yang Yunze Yang Shuzeng Wang lingjun Shi Wenhai Xie Kejing Sun Kang Zou Lei Wang Ji Xiong jie Xiang Ji Wu School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityNo.800 Dongchuan RoadMinhang DistrictShanghai 200240China

Oocyte production in most mammalian species is believed to cease before ***,this idea has been challenged with the finding that postnatal mouse ovaries possess mitotically active germ cells.A recent study showed that female germline stem cells(FGSCs)from adult mice were isolated,cultured long term and produced oocytes and progeny after transplantation into infertile ***,we demonstrate the successful generation of transgenic or gene knock-down mice using *** FGSCs from ovaries of 5-day-old and adult mice were isolated and either infected with recombinant viruses carrying green fluorescent protein,Oocyte-G1 or the mouse dynein axonemal intermediate chain 2 gene,or transfected with the Oocyte-G1 specific shRNA expression vector(pRS shOocyte-G1 vector),and then transplanted into infertile *** cells in the ovaries underwent oogenesis and produced heterozygous offspring after mating with wild-type male *** offspring were genetically characterized and the biological functions of the transferred or knock-down genes were *** of genetransfer or gene knock-down was 29%–37%and it took 2 months to produce transgenic *** manipulation of FGSCs is a rapid and efficient method of animal transgenesis and may serve as a powerful tool for biomedical science and biotechnology.

关键词： female germline stem cells gene-transfer knock-down Oocyte-G1 mouse dynein axonemal intermediate chain 2(Dnaic2)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Glucocorticoid receptor modulates myeloid-derived suppressor cell function via mitochondrial metabolism in immune thrombocytopenia

引用

Cellular & Molecular Immunology 2022年第7期19卷 764-776页

作者： Yu Hou jie Xie Shuwen Wang Daqi Li lingjun Wang Haoyi Wang Xiaofei Ni Shaoqiu Leng Guosheng Li Ming Hou Jun Peng Department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Shandong Provincial Key Laboratory of Immunohematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Jinan Central Hospital Cheeloo College of MedicineShandong UniversityJinanChina Leading Research Group of Scientific Innovation Department of Science and Technology of Shandong ProvinceQilu HospitalCheeloo College of MedicineShandong UniversityJinanChina Advanced Medical Research Institute Shandong UniversityJinanChina

Myeloid-derived suppressor cells(MDSCs)are a heterogeneous population of immature cells and natural inhibitors of adaptive *** metabolic changes in MDSCs exert a direct immunological influence on their suppressive *** previous study demonstrated that high-dose dexamethasone(HD-DXM)corrected the functional impairment of MDSCs in immune thrombocytopenia(ITP);however,the MDSC population was not restored in nonresponders,and the mechanism remained *** this study,altered mitochondrial physiology and reduced mitochondrial gene transcription were detected in MDSCs from HD-DXM nonresponders,accompanied by decreased levels of carnitine palmitoyltransferase-1(CPT-1),a rate-limiting enzyme in fatty acid oxidation(FAO).Blockade of FAO with a CPT-1 inhibitor abolished the immunosuppressive function of MDSCs in HD-DXM *** also report that MDSCs from ITP patients had lower expression of the glucocorticoid receptor(GR),which can translocate into mitochondria to regulate the transcription of mitochondrial DNA(mtDNA)as well as the level of oxidative *** was confirmed that the expression of CPT-1 and mtDNA-encoded genes was downregulated in GR-siRNA-treated murine ***,by establishing murine models of active and passive ITP via adoptive transfer of DXM-modulated MDSCs,we confirmed that GR-silenced MDSCs failed to alleviate thrombocytopenia in mice with *** conclusion,our study indicated that impaired aerobic metabolism in MDSCs participates in the pathogenesis of glucocorticoid resistance in ITP and that intact control of MDSC metabolism by GR contributes to the homeostatic regulation of immunosuppressive cell function.

关键词： immune thrombocytopenia myeloid-derived suppressor cell HD-DXM glucocorticoid receptor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The correlation of drug resistance and virulence in Mycobacterium tuberculosis

引用

Biosafety and Health 2020年第1期2卷 18-24页

作者： Zhan lingjun Wang jie Wang Liang Qin Chuan Institute of Laboratory Animal Science Chinese Academy of Medical Sciences(CAMS)Beijing 100021China Key Laboratory of Human Diseases Animal Model State Administration of Traditional Chinese MedicineBeijing 100021China Key Laboratory of Human Disease Comparative Medicine Ministry of HealthBeijing Key Laboratory for Animal Models of Emerging and Reemerging InfectiousBeijing 100021China Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing 100021China Tuberculosis(TB)Center Chinese Academy of Medical SciencesBeijing 100021China Department of Bioinformatics School of Medical Informatics and EngineeringXuzhou Medical UniversityXuzhou 221000China

The low success rates in the treatment of multidrug-resistant tuberculosis(MDR-TB)and extensively drug-resistant TB(XDR-TB),which account for 55%and 34%respectively,led the WHO to conclude that MDR/XDR-TB is a serious public health ***,the virulence of MDR/XDR-Mycobacterium Tuberculosis(Mtb)has not been analyzed in details,which could provide a specific guidance for the control and *** this review,we discuss different aspects of MDR/XDR-Mtb virulence and its relationship to fitness cost by probing the following questions:(1)what mediates the virulence of MDR/XDR-Mtb?(What is the relationship between fitness and virulence of Mtb?(2)Is it possible that drug-resistant Mtb(DR Mtb)can show higher fitness?(3)What is the definite effect on fitness of each drug-resistant mutant?(4)What other important factors affecting fitness in the mutant strain?(5)How to study the virulence of a large number of DR Mtb?And what prevention and control measures will be taken in the future,especially for the high virulent DR Mtb?We therefore summarized the congruent relationship between drug resistance and fitness from the global response-related genes to antibiotic resistance-contributing mutation,provided methods to explore the virulence of DR *** review may offer some critical information and concise guide to creating strategies for the prevention and control of drug-resistant Mtb.

关键词： Tuberculosis Drug resistance Mutation Global response-related gene Virulence Fitness Correlation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

旋转机械同源数据全信息倒频谱分析与应用

引用

机械强度 2005年第4期27卷 452-455页

作者：韩捷董辛旻郝伟李凌均郑州大学振动工程研究所郑州450002

提出一种基于旋转机械同源数据融合的全信息倒频谱(fullinformationcepstrum,FIC)分析方法。研究旋转机械同源信息融合的转子回转能量谱,并在此基础上研究全信息倒频谱分析方法。成功地将全信息分析理念拓展到频域分析以外的分析领域,... 详细信息

提出一种基于旋转机械同源数据融合的全信息倒频谱(fullinformationcepstrum,FIC)分析方法。研究旋转机械同源信息融合的转子回转能量谱,并在此基础上研究全信息倒频谱分析方法。成功地将全信息分析理念拓展到频域分析以外的分析领域,将其应用于高速旋转动力齿轮的故障诊断,取得明显的效果。

关键词：同源信息全信息倒谱数据融合故障诊断

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：