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DNA methylation changes detected by methylation-sensitive amplified polymorphism in two contrasting rice genotypes under salt stress

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Journal of Genetics and Genomics 2011年第9期38卷 419-424页

作者： Wensheng Wang Xiuqin Zhao Yajiao Pan linghua zhu Binying Fu Zhikang Li Institute of Crop Sciences National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China International Rice Research Institute DAPO Box 7777 Metro Manila Philippines

DNA methylation,one of the most important epigenetic phenomena,plays a vital role in tuning gene expression during plant development as well as in response to environmental stimuli.In the present study,a methylation-sensitive amplified polymorphism（MSAP） analysis was performed to profile DNA methylation changes in two contrasting rice genotypes under salt stress.Consistent with visibly different phenotypes in response to salt stress,epigenetic markers classified as stable inter-cultivar DNA methylation differences were determined between salt-tolerant FL478 and salt-sensitive IR29.In addition,most tissue-specific DNA methylation loci were conserved,while many of the growth stage-dependent DNA methylation loci were dynamic between the two genotypes.Strikingly,salt stress induced a decrease in DNA methylation specifically in roots at the seedling stage that was more profound in IR29 than in the FL478.This result may indicate that demethylation of genes is an active epigenetic response to salt stress in roots at the seedling stage,and helps to further elucidate the implications of DNA methylation in crop growth and development.

关键词： DNA methylation MSAP Salt tolerance Rice

Selection efficiencies for improving drought/salt tolerances and yield using introgression breeding in rice(Oryza sativa L.)

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The Crop Journal 2013年第2期1卷 134-142页

作者： Ying Wang Lubiao Zhang Afif Nafisah linghua zhu Jianlong Xu Zhikang Li Institute of Crop Science The National Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences

The backcross(BC) breeding strategy has been increasingly used for developing high yielding varieties with improved abiotic stress tolerances in rice. In this study, 189Huang-Hua-Zhan(HHZ) introgression lines(ILs) developed from three different selection schemes were evaluated for yield related traits under drought stress and non-stress conditions in the target and off-season winter nursery environments to assess the selection efficiency of BC breeding for improving different complex traits, and led us to five important results. The first result indicated that the primary target traits should be selected first in the target environments(TEs) in order to achieve the maximum genetic gain. Secondly, BC breeding for drought tolerance(DT) in rice was almost equally effective by strong phenotypic selection in the main target environments and in the winter-season of Hainan.Thirdly, exploiting genetic diversity in the subspecific gene pools is of great importance for future genetic improvement of complex traits in rice. Fourthly, considerable genetic gain can be effectively achieved by selection for secondary target traits among the ILs with the primary traits. Finally, the developed ILs provide useful materials for future genetic/genomic dissection and molecular breeding of complex traits.

关键词： Backcross breeding High yield Drought tolerance Salt tolerance Selection efficiency

一项基于全国多中心前瞻性登记队列研究(PACAGE)的中国胃癌和结直肠癌患者术后并发症现状分析

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中华胃肠外科杂志 2024年第3期27卷 247-260页

作者：张书勤吴舟桥霍博文徐惠宁赵康靖昌庆刘凤林余江李正荣张健臧潞蒿汉坤郑朝辉李勇樊林黄华梁品吴斌朱甲明牛兆建朱玲华宋武尤俊燕速李子禹青海大学医学部公共卫生系青海大学附属医院西宁810001 北京大学肿瘤医院胃肠肿瘤中心北京100142 青海大学附属医院胃肠肿瘤外科西宁810001 山东第一医科大学附属省立医院胃肠外科济南250021 复旦大学附属肿瘤医院胃外科上海200025 南方医科大学附属南方医院普通外科广州510515 南昌大学附属第一医院胃肠外科南昌330006 浙江大学附属第一医院胃肠外科杭州310003 上海交通大学医学院附属瑞金医院胃肠外科上海200025 复旦大学附属华山医院胃肠外科上海200040 福建医科大学附属协和医院胃外科福州350001 广东省人民医院胃肠外科广州510080 西安交通大学附属第一医院普通外科西安710061 大连医科大学附属第一医院胃肠外科大连116011 中国协和医科大学附属协和医院基本外科北京100032 中国医科大学第一附属医院胃肠肿瘤外科沈阳110002 青岛大学附属医院胃肠外科青岛266000 浙江大学附属邵逸夫医院胃肠外科杭州310009 中山大学第一附属医院胃肠外科广州510062 厦门大学第一附属医院胃肠肿瘤外科厦门361003 徐州医科大学附属宿迁医院感染管理科

目的探讨我国胃癌和结直肠癌患者术后并发症的发生率,并评估其危险因素。方法本研究采用全国多中心前瞻性数据登记队列研究方法,数据来自中国胃肠肿瘤外科联盟发起的胃肠外科手术后腹部并发症现状研究数据库(PACAGE)。PACAGE数据库自201... 详细信息

目的探讨我国胃癌和结直肠癌患者术后并发症的发生率,并评估其危险因素。方法本研究采用全国多中心前瞻性数据登记队列研究方法,数据来自中国胃肠肿瘤外科联盟发起的胃肠外科手术后腹部并发症现状研究数据库(PACAGE)。PACAGE数据库自2018年12月至2020年12月期间,前瞻性收集了20家医学中心胃癌和结直肠癌患者一般人口统计学数据、围手术期治疗及术后并发症相关数据,根据是否发生术后并发症进行分组,参照胃肠肿瘤外科术后并发症相关专家共识和Clavien-Dindo分级标准,对并发症进行分类及分级。通过绘制热图,将不同分级的术后并发症发生率可视化;采用多因素非条件logistic回归模型,分析发生术后并发症的独立危险因素。结果共纳入3926例胃癌和结直肠癌患者,657例患者共发生876例次术后并发症,总体并发症发生率16.7%(657/3926),Ⅲ级及以上严重并发症发生率4.0%(156/3926),Ⅴ级并发症发生率0.2%(7/3926)。胃癌患者2271例,术后并发症发生率18.1%(412/2271),严重并发症发生率4.7%(106/2271);结直肠癌患者1655例,术后并发症发生率14.8%(245/1655),严重并发症发生率3.0%(50/1655)。胃癌和结直肠癌患者吻合口漏的发生率分别是3.3%(74/2271)和3.4%(56/1655)。腹腔感染在各类并发症中占比最高,在胃癌和结直肠癌患者中分别为28.7%(164/572)和39.5%(120/304)。在所有术后并发症严重程度分级中,Ⅱ级并发症占比最高,胃癌和结直肠癌分别占65.4%(374/572)和56.6%(172/304)。多因素分析显示:(1)胃癌组患者发生术后并发症的独立影响因素包括:有术前合并症(OR=2.54,95%CI:1.51~4.28,P180 min(OR=1.81,95%CI:1.42~2.31,P50 ml(OR=1.29,95%CI:1.01~1.63,P=0.038)和相比于全胃切除术的远端胃切除(OR=0.65,95%CI:0.51~0.83,P<0.001);(2)肠癌组患者发生术后并发症的独立影响因素包括:女性(OR=0.60,95%CI:0.44~0.80,P<0.001)、有术前合并症(OR=2.73,95%CI:1.25~5.99,P=0.030)、接受新辅助治疗(OR=1.83,95%CI:1.23~2.72,P=0.008)、腹腔镜手术(OR=0.47,95%CI:0.30~0.72,P=0.022)和相比于低位前切术的腹会阴联合切术(OR=2.74,95%CI:1.71~4.41,P<0.001)。结论胃癌和结直肠癌患者术后各类感染相关的并发症最为常见,虽然两者术后发生并发症的危险因素各有不同,但有术前合并症、行新辅助治疗以及手术切除范围,是胃癌和结直肠癌患者术后发生并发症的共同影响因素。

关键词：胃肿瘤结直肠肿瘤术后并发症风险因素

Broadband and polarization-independent arbitrary ratio integrated optical power splitter built on thick silicon nitride platform

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Optoelectronics Letters 2024年

作者： ZHENG Langteng CHEN Yiqiang XUE Zhengqun HUANG Jiwei zhu Minmin WANG linghua College of Physics and Information Engineering Fuzhou University

Integrated optical power splitters are basic but indispensable on-chip devices in silicon photonics. They can be used either for power distribution or monitoring， or as the building blocks for more complex devices or circuits. Although different types of optical power splitters with different architectures have been proposed and demonstrated， devices that could work with arbitrary power splitting ratio in a large bandwidth without polarization dependence， are still rare to be seen. In this paper， we propose and investigate an optical power splitter with adiabatically tapered waveguide structures on a thick silicon nitride platform， which could meet the requirement mentioned above. With optimized structural parameters obtained by three-dimensional finite-difference time-domain simulation， the polarization dependence of different power splitting ratio gets almost eliminated for each specific working wavelength. In a broad wavelength range (1340-1800nm)， the IL of the device is below 1dB， and the variation of the PSR can be controlled within ～±5% if compared with the targeted design value for 1550nm centered wavelength. Simple structure， relaxed critical dimensions， and good fabrication tolerance make this device compatible with the standard fabrication process in commercial silicon photonic foundries.

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Deep transcriptome sequencing of rhizome and shoot in Sorghum propinquum

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Deep transcriptome sequencing of rhizome and shoot in Sorghu...

Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance at Anthesis in Rice (Oryza sativa L.)

第十四届全国植物基因组学大会

作者： Ting Zhang Xiuqin Zhao Wensheng Wang Liyu Huang Xiaoyue Liu Ying Zong linghua zhu Daichang Yang Binying Fu Zhikang Li Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

Transcriptomic data for Sorghum propinquum,the wild-type sorghum,are limited in public databases.S.propinquum has a subterranean rhizome and transcriptome data will help in understanding the molecular mechanisms under... 详细信息

关键词： Sorghum propinquum transcriptome Illumina sequencing shoot rhizome

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Journal of Integrative Agriculture 2012年第3期11卷 359-367页

作者： CHENG Li-rui WANG Jun-min Veronica Uzokwe MENG Li-jun WANG Yun SUN Yong zhu linghua XU Jian-long LI Zhi-kang National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Zhejiang Academy of Agricultural Sciences Hangzhou 310021P.R.China International Rice Research Institute DAPO Box 7777Metro ManilaPhilippines

A set of 240 introgression lines derived from the advanced backcross population of a cross between a japonica cultivar,Xiushui 09,and an indica breeding line,IR2061,was developed to dissect QTLs affecting cold tolerance （CT） at seedling stage and heat tolerance （HT） at anthesis.Survival rate of seedlings （SRS） and spikelet fertility （SF）,the index traits of CT and HT,showed significant differences between the two parents under stresses.A total of four QTLs （qSRS1,qSRS7,qSRS11a and qSRS11b） for CT were identified on chromosomes 1,7,11,and the Xiushui 09 alleles increased SRS at all loci except qSRS7.Four QTLs for SF were identified on chromosomes 4,5,6,and 11.These QTLs could be classified into two major types based on their behaviors under normal and stress conditions.The first was QTL expressed only under normal condition;and the second QTL was apparently stress induced and only expressed under stress.Among them,two QTLs （qSF4 and qSF6） which reduced the trait difference between heat stress and normal conditions must have contributed to HT because of their obvious contribution to trait stability,and the IR2061 allele at the qSF6 and the Xiushui 09 allele at the qSF4 improved HT,respectively.No similar QTL was found between CT at seedling stage and HT at anthesis.Therefore,it is possible to breed a new variety with CT and HT by pyramiding the favorable CT-and HT-improved alleles at above loci from Xiushui 09 and IR2061,respectively,through marker-assisted selection （MAS）.

关键词： cold tolerance heat tolerance advanced backcross population QTL mapping rice

三亚河水体中甲烷和氧化亚氮的分布、释放及影响因素研究

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中国环境监测 2023年第2期39卷 117-124页

作者：韩玉李霞郑忠陆陈贤伟郭雨昂朱柃桦石晓然海南热带海洋学院海洋科学技术学院海南三亚572000 海南热带海洋学院生态环境学院海南三亚572000

甲烷(CH_(4))和氧化亚氮(N_(2)O)是大气中重要的温室气体,对全球变暖和大气化学有重要作用。受人为活动影响较大的河流、河口是大气中CH_(4)和N_(2)O的重要源。于2019年春季、2020年秋季台风前后沿三亚河从下游到上游采集了11个站位的样... 详细信息

甲烷(CH_(4))和氧化亚氮(N_(2)O)是大气中重要的温室气体,对全球变暖和大气化学有重要作用。受人为活动影响较大的河流、河口是大气中CH_(4)和N_(2)O的重要源。于2019年春季、2020年秋季台风前后沿三亚河从下游到上游采集了11个站位的样品,测定三亚河水体中CH_(4)、N_(2)O浓度及相关参数,并估算了水-气界面释放通量。结果表明:春季、秋季以及台风后CH_(4)平均浓度分别为(15.92±23.04)、(364.43±265.41)、(666.02±502.60) nmol/L,N_(2)O平均浓度分别为(24.37±19.88)、(8.47±5.19)、(47.48±33.47) nmol/L,台风后大于台风前。水体中CH_(4)、N_(2)O主要受溶解氧(DO)和降雨影响,下游河段主要受潮汐作用影响。现场产生和沉积物释放是水体中CH_(4)的主要源,硝化作用是水体中N_(2)O的主要源。三亚河水体中溶存的CH_(4)和N_(2)O均处于过饱和状态,每年向大气释放的CH_(4)和N_(2)O的量分别约为0.040~58.04 Gg和-0.065~3.8 Gg,是大气中N_(2)O和CH_(4)的净源。

关键词：甲烷氧化亚氮水-气交换通量影响因素

右侧微创三孔法腹腔镜袖状胃切除术治疗肥胖症的应用价值

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中华消化外科杂志 2022年第11期21卷 1461-1466页

作者：骆鹏陈国俊陈炯煌齐永强童一凡朱玲华于卫华浙江大学医学院附属邵逸夫医院普通外科杭州310020

目的探讨右侧微创三孔法腹腔镜袖状胃切除术(RMIT‑LSG)治疗肥胖症的应用价值。方法采用回顾性描述性研究方法。收集2021年1—10月浙江大学医学院附属邵逸夫医院收治的66例肥胖症行RMIT‑LSG患者的临床资料;男15例,女51例;年龄为28.5(16.0... 详细信息

目的探讨右侧微创三孔法腹腔镜袖状胃切除术(RMIT‑LSG)治疗肥胖症的应用价值。方法采用回顾性描述性研究方法。收集2021年1—10月浙江大学医学院附属邵逸夫医院收治的66例肥胖症行RMIT‑LSG患者的临床资料;男15例,女51例;年龄为28.5(16.0~54.0)岁,体质量指数(BMI)为(36.9±4.3)kg/m^(2);其中20例患者合并2型糖尿病。观察指标:(1)手术情况。(2)术后情况。(3)随访情况。通过门诊或微信方式进行随访,了解患者术后恢复情况,术后6个月随访患者体质量变化情况、BMI和并发症情况。随访时间截至2021年12月。正态分布的计量资料以x±s表示,偏态分布的计量资料以M(范围)表示,计数资料以绝对数表示。结果(1)手术情况。66例患者均完成RMIT‑LSG,无中转开腹或更改手术方式;手术时间为(132±22)min,术中出血量为(14±8)mL。(2)术后情况。66例患者术后首次下床活动时间为(15±6)h,术后首次肛门排气时间为(1.80±0.60)d,术后首次饮水时间为(1.00±0.20)d,术后首次进食流质食物时间为(2.00±0.20)d,术后住院时间为(3.40±0.60)d。66例患者中,1例术后第1天出现腹腔出血,予二次手术止血,恢复顺利;其余均恢复良好,无胃瘫、胃瘘、腹腔感染等并发症。(3)随访情况。66例患者术后均获得随访,随访时间为6(1~11)个月。术后1个月随访时,66例患者常规行术后瘢痕摄像,瘢痕隐蔽具有较好美容效果。27例患者随访时间6个月,体质量降低(42±7)kg,减重比例达34.8%±2.9%,BMI降低(14.2±1.9)kg/m^(2);66例患者随访期间未发现营养不良。结论RMIT‑LSG治疗肥胖症安全、有效,切口美容效果较好。

关键词：肥胖症减重手术减重代谢外科右侧微创三孔法袖状胃切除术腹腔镜检查

小檗胺对糖尿病肾病大鼠肾损伤和炎性细胞因子产生的影响

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免疫学杂志 2020年第7期36卷 599-605页

作者：石磊向海燕朱玲华湖北民族学院附属民大医院肾内科恩施445000

目的研究小檗胺(berbamine,BBM)对糖尿病肾病(diabetic nephropathy,DN)大鼠的保护作用及其机制。方法将大鼠分为对照组组、模型组、DN+BBM组(50、100、200 mg/kg),采用高脂喂食结合化学诱导法建立DN大鼠模型。检测24 h尿蛋白含量,检测... 详细信息

目的研究小檗胺(berbamine,BBM)对糖尿病肾病(diabetic nephropathy,DN)大鼠的保护作用及其机制。方法将大鼠分为对照组组、模型组、DN+BBM组(50、100、200 mg/kg),采用高脂喂食结合化学诱导法建立DN大鼠模型。检测24 h尿蛋白含量,检测血清肌酸酐(SCr)和尿素氮(BUN);苏木精-伊红(HE)和TUNEL染色观察肾脏病理情况;Western blot检测凋亡、炎症通路相关蛋白表达;ELISA检测血清中炎症细胞因子表达。建立LPS+DN大鼠模型,检测指标同上。结果与模型组相比,DN+BBM组大鼠尿蛋白、血清肌酐和尿素氮含量、肾组织病理损伤、凋亡率、cl-caspase-3、cl-caspase-9和Bax蛋白表达、炎症细胞因子IL-1β、IL-6和TNF-α含量、炎症通路蛋白p-P65、MIP-2表达均降低;Bcl-2表达增加。此外,BBM显著减弱LPS对DN大鼠肾脏功能、凋亡率及相关蛋白表达、血清炎性细胞因子及炎症通路蛋白表达的调节作用。结论小檗胺能有效缓解糖尿病肾病大鼠肾损伤和炎症反应,其机制与抑制NF-κB p65活化有关。

关键词：小檗胺糖尿病肾病炎症通路