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The Crop Journal 2023年第3期11卷 774-784页

作者： Jianyan Zeng Dan Yao Ming Luo Lingli Ding Yi Wang Xingying Yan Shu’e Ye Chuannan Wang Yiping Wu Jingyi Zhang Yaohua Li lingfang ran Yonglu Dai Yang Chen Fanlong Wang Hanyan Lai Nian Liu Nianjuan Fang Yan Pei Yuehua Xiao Biotechnology Research Center Southwest UniversityChongqing 400715China

Cotton fiber is a raw material for the global textile industry and fiber quality is essential to its industrial *** are plant secondary metabolites that may serve as dietary components,regulate light harvesting,and scavenge reactive oxygen *** carotenoids accumulate predominantly in rapidly elongating cotton fibers,their roles in cotton fiber development remain poorly *** this study,a fiber-specific promoter proSCFP was applied to drive the expression of GhOR1Del,a positive regulator of carotenoid accumulation,to upregulate the carotenoid level in cotton fiber in *** length,strength,and fineness were increased in proSCFP:GhOR1Del transgenic cotton and abscisic acid(ABA)and ethylene contents were increased in elongating *** ABA downstream regulator GhbZIP27a stimulated the expression of the ethylene synthase gene GhACO3 by binding to its promoter,suggesting that ABA promoted fiber elongation by increasing ethylene *** findings suggest the involvement of carotenoids and ABA signaling in promoting cotton fiber elongation and provide a strategy for improving cotton fiber quality.

关键词： Abscisic acid Carotenoid Cotton fiber elongation Ethylene OranGE gene

Upregulation of the glycine-rich protein-encoding gene GhGRPL enhances plant tolerance to abiotic and biotic stressors by promoting secondary cell wall development

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Journal of Integrative Agriculture 2024年第10期23卷 3311-3327页

作者： Wanting Yu Yonglu Dai Junmin Chen Aimin Liang Yiping Wu Qingwei Suo Zhong Chen Xingying Yan Chuannan Wang Hanyan Lai Fanlong Wang Jingyi Zhang Qinzhao Liu Yi Wang Yaohua Li lingfang ran Jie Xiang Zhiwu Pei Yuehua Xiao Jianyan Zeng College of Agronomy and Biotechnology Southwest UniversityChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Chongqing Key Laboratory of Crop Molecular Improvement Chongqing 400715China Academy of Agricultural Sciences Southwest UniversityChongqing 400715China

Abiotic and biotic stressors adversely affect plant survival,biomass generation,and crop *** the global availability of arable land declines and the impacts of global warming intensify,such stressors may have increasingly pronounced effects on agricultural ***,researchers face the overarching challenge of comprehensively enhancing plant resilience to abiotic and biotic *** secondary cell wall plays a crucial role in bolstering the stress resistance of *** increase plant resistance to stress through genetic manipulation of the secondary cell wall,we cloned a cell wall protein designated glycine-rich protein-like(GhGRPL)from cotton fibers,and found that it is specifically expressed during the period of secondary cell wall ***,this protein differs from its Arabidopsis homolog,AtGRP,since its glycine-rich domain is deficient in glycine *** is involved in secondary cell wall *** of GhGRPL enhances lignin accumulation and,consequently,the thickness of the secondary cell walls,thereby increasing the plant’s resistance to abiotic stressors,such as drought and salinity,and biotic threats,including Verticillium dahliae ***,interference with GhGRPL expression in cotton reduces lignin accumulation and compromises that *** together,our findings elucidate the role of GhGRPL in regulating secondary cell wall development through its influence on lignin deposition,which,in turn,reinforces cell wall robustness and *** findings highlight the promising near-future prospect of adopting GhGRPL as a viable,effective approach for enhancing plant resilience to abiotic and biotic stress factors.

关键词： glycine-rich protein-like secondary cell wall abiotic stress biotic stress stress resistance

IMCI: an efficient fingerprint retrieval approach based on 3D stacked memory

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Science China(Information Sciences) 2020年第7期63卷 233-235页

作者： Wen CHENG ran CAI lingfang ZENG Dan FENG André BRINKMANN Yang WANG Wuhan National Laboratory for Optoelectronics School of Computer Science and TechnologyHuazhong University of Science and Technology Department of Computer Science Johannes Gutenberg-University Mainz Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

Dear editor,Data deduplication (Dedup for short) as a type of redundant data elimination technology, can effectively reduce the impact of redundant data on storage costs, which consequently alleviates the problems and... 详细信息

关键词： data deduplication fingerprint retrieval process in memory 3D stacked memory storage design

Ball Milling Synthesis and Property of Nd_(0.7)Sr_(0.3)MnO_3 Manganites

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Journal of Materials Science & Technology 2010年第8期26卷 721-724页

作者： Shunsheng Chen Changping Yang lingfang Xu Shaolong Tang The Provincial Key Laboratory of Piezoelectric Ceramics Materials and Apparatus Faculty of Physics and Electronic Technology Hubei University Wuhan 430062 China Department of Physics Nanjing University Nanjing 210093 China

Strontium doped perovskite-type Nd0.7Sr0.3MnO3 ceramics were synthesized completely by high-energy ball milling raw oxides of Nd2O3, SrCO3 and MnO2. The optimal ball milling time and mass ratio of milling balls to raw materials are 4 h and 10：1, respectively. The grain size of as-milled Nd0.7Sr0.3MnO3 ceramics ranges from 51 to 93 nm, and the fine particles contain two phases of crystalline phase and amorphous phase. For the Nd0.7r0.3MnO3 synthesized by ball milling and sequent heat treatment, a remarkable colossal electroresistance （CER） effect is observed and the CER ratio reaches 900% at Curie temperature Tc when the load voltage increases from 0.1 to 0.8 V.

关键词： High-energy ball milling Perovskite structure Colossal electroresistance (CER) Microstructure defect

Heat contribution of the Indonesian throughflow to the Indian Ocean

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Acta Oceanologica Sinica 2019年第4期38卷 72-79页

作者： Tiecheng Zhang Weiqiang Wang Qiang Xie lingfang Chen Institute of Deep-sea Science and Engineering Chinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of Sciences Laboratory for Regional Oceanography and Numerical Modeling Pilot National Laboratory for Marine Science and Technology (Qingdao)

Based on the high-resolution Eulerian fields of an ocean general circulation model simulation, the heat contribution of the Indonesian throughflow(ITF) to the Indian Ocean is estimated by Lagrangian tracing *** heat transport of each particle of ITF waters is calculated by tracing temperature change along the trajectory until the particle exits the Indian Ocean. The simulation reveals that the ITF waters flow westward and branch near Madagascar, further showing the ITF waters are redistributed in both northern and southern Indian *** budget analysis indicates that the ITF waters gain 0.41 PW(Petawatts, 1015 W) in the northern Indian Ocean and lose 0.56 PW in the southern Indian Ocean, respectively. As a result, the ITF waters warm the whole Indian Ocean basin with only 0.15 PW, which shows an "insignificant" role of ITF on the Indian Ocean because of the heat exchange compensation between northern and southern Indian Ocean. Furthermore, the tracing pathways show that the ITF waters mainly flow out the Indian Ocean at both sides of the basin via Agulhas Current and Leeuwin Current. About 89% of the ITF waters leave along western boundary and the rest 11% along eastern boundary. Compared to seeding section, 0.10 PW and 0.05 PW are released to the Indian Ocean, respectively.

关键词： Indonesian throughflow Indian Ocean heat contribution

Exploiting flash memory characteristics to improve performance of RAIS storage systems

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Frontiers of Computer Science 2019年第5期13卷 913-928页

作者： Linjun MEI Dan FENG lingfang ZENG Jianxi CHEN Jingning LIU Wuhan National Laboratory for Optoelectronics School of Computer Huazhong University of Science and Technology Wuhan 430074 China

Redundant array of independent SSDs (RAIS) is generally based on the traditional RAID design and implementation. The random small write problem is a serious challenge of RAIS. random small writes in parity-based RAIS systems generate significantly more pre-reads and writes which can degrade RAIS performance and shorten SSD lifetime. In order to overcome the well-known write-penalty problem in the parity-based RAID5 storage systems, several logging techniques such as Parity Logging and Data Logging have been put forward. However, these techniques are originally based on mechanical characteristics of the HDDs, which ignore the properties of the flash memory. In this article, we firstly propose RAISL, a flash-aware logging method that improves the small write performance of RAIS storage systems. RAISL writes new data instead of new data and pre-read data to the log SSD by making full use of the invalid pages on the SSD of RAIS. RAISL does not need to perform the pre-read operations so that the original characteristics of workloads are kept. Secondly, we propose AGCRL on the basis of RAISL to further boost performance. AGCRL combines RAISL with access characteristic to guide read and write cost regulation to improve the performance of RAIS storage systems. Our experiments demonstrate that the RAISL significantly improves write performance and AGCRL improves both of write performance and read performance. AGCRL on average outperforms RAIS5 and RAISL by 39.15% and 16.59% respectively.

关键词： solid state drives RAIS properties performance

Effects of all-trans retinoic acid on proliferation of human aorta smooth muscle cells

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Chinese Science Bulletin 1999年第5期44卷 448-451页

作者： Guoxia Liu Cunxi Li lingfang Zeng Yongchao Wang Beijing Normal Univ Key Lab Cell Proliferat & Regulat Biol Beijing 100875 Peoples R China

The primary cultured human aorta smooth muscle cells(SMCs)were characterized and theeffects of all—trans retinoic acid(atRA)on these cells were *** is found that atRA could slowdown the growth rate of SMCs,and decrease the expression Ievels of cyclinDl,CDK4,c-myc at39％。38％and 43.6％respectively,while atRA had no effects on the expression of c^-actin which isspecific for *** demonstrated that atRA inhibited cell growth of SMCs but did not induce *** effects may be clue to the decreased translation Ievels of ***4 andtranscription Ievel of *** fact that ati tA inhibited SMCs proIiferation may become one of thetheoretic basis for the cIinicaI use of atRA in the trea㈣t of SMCs proliferative diseases such asatherosclerosis.

关键词： SMC RA proliferation inhibition.

Interaction between Rice stripe virus Disease- Specific Protein and Host PsbP Enhances Virus Symptoms

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Molecular Plant 2014年第4期7卷 691-708页

作者： lingfang Kong Jianxiang Wu Lina Lu Yi Xu Xueping Zhou State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 People's Republic of China

Rice stripe virus （RSV） causes severe diseases in Oryza sativa （rice） in many Eastern Asian countries. Diseasespecific protein （SP） of RSV is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of RSV symptoms. Here, we present evidence that expression of RSV SP alone in rice or Nicotiana benthamiana did not produce visible symptoms. Expression of SP in these two plants, however, enhanced RSV- or Potato virus X （PVX）- induced symptoms. Through yeast two-hybrid screening, GST pull-down, and bimolecular fluorescence complementation assays, we demonstrated that RSV SP interacted with PsbP, a 23-kDa oxygen-evolving complex protein, in both rice and N. benthamiana. Furthermore, our investigation showed that silencing of PsbP expression in both plants increased disease symptom severity and virus accumulation. Confocal microscopy using N, benthamiana protoplast showed that PsbP accu- mulated predominantly in chloroplast in wild-type N. benthamiana cells. In the presence of RSV SP, most PsbP was recruited into cytoplasm of the assayed cells. In addition, accumulation of SP during RSV infection resulted in alterations of chloroplast structure and function. Our findings shed light on the molecular mechanism underlying RSV disease symptom development.

关键词： Rice stripe virus disease-specific protein interaction PsbP symptom.

Alleviated photoinhibition on nitrification in the Indian Sector of the Southern Ocean

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Acta Oceanologica Sinica 2024年第7期43卷 52-69页

作者： lingfang Fan Min Chen Zifei Yang Minfang Zheng Yusheng Qiu College of Ocean and Earth Sciences Xiamen UniversityXiamen 361102China

Nitrification,a central process in the marine nitrogen cycle,produces regenerated nitrate in the euphotic zone and emits N_(2)O,a potent greenhouse gas as a *** regulatory mechanisms of nitrification in the Southern Ocean,which is a critical region for CO_(2)sequestration and radiative benefits,remain poorly ***,we investigated the in situ and dark nitrification rates in the upper 500 m and conducted substrate kinetics experiments across the Indian Sector in the Cosmonaut and Cooperation seas in the late austral *** findings indicate that light inhibition of nitrification decreases exponentially with depth,exhibiting a light threshold of 0.53%photosynthetically active radiation.A positive relationship between dark nitrification and apparent oxygen utilization suggests a dependence on substrate availability from primary ***,an increased NH_(4)^(+) supply can act as a buffer against photo-inhibitory ***,substrate affinity(α)increases with depth and transitions from light to dark,decreases with increasing ambient NH_(4)^(+)and exhibits a latitudinal distribution,reflecting substrate utilization *** also reveal that upwelling in Circumpolar Deep Water(CDW)stimulates nitrification through the introduction of potentially higher iron and deep diverse nitrifying microorganisms with higherα.We conclude that although light is the primary limiting factor for nitrification in summer,coupling between substrate availability and CDW upwelling can overcome this limitation,thereby alleviating photoinhibition by up to 45%±5.3%.

关键词： nitrification light inhibition substrate affinity circumpolar deep water(CDW)upwelling the Southern Ocean

Multi-scale variability of the tropical Indian Ocean circulation system revealed by recent observations

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Science China Earth Sciences 2018年第6期61卷 668-680页

作者： Ke HUANG Dongxiao WANG Weiqiang WANG Qiang XIE Ju CHEN lingfang CHEN Gengxin CHEN State Key Laboratory of Tropical Oceanography South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Institute of Deep Sea Science and Engineering Chinese Academy of Sciences Sanya 572000 China Laboratory for Regional Oceanography and Numerical Modeling Qingdao National Laboratory for Marine Science and Technology Qingdao 266237 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

The tropical Indian Ocean circulation system includes the equatorial and near-equatorial circulations, the marginal sea circulation, and eddies. The dynamic processes of these circulation systems show significant multi-scale variability associated with the Indian Monsoon and the Indian Ocean dipole. This paper summarizes the research progress over recent years on the tropical Indian Ocean circulation system based on the large-scale hydrological observations and numerical simulations by the South China Sea Institute of Oceanology(SCSIO), Chinese Academy of Sciences. Results show that:(1) the wind-driven Kelvin and Rossby waves and eastern boundary-reflected Rossby waves regulate the formation and evolution of the Equatorial Undercurrent and the Equatorial Intermediate Current;(2) the equatorial wind-driven dynamics are the main factor controlling the inter-annual variability of the thermocline in the eastern Indian Ocean upwelling;(3) the equatorial waves transport large amounts of energy into the Bay of Bengal in forms of coastal Kelvin and reflected free Rossby waves. Several unresolved issues within the tropical Indian Ocean are discussed:(i) the potential effects of the momentum balance and the basin resonance on the variability of the equatorial circulation system, and(ii) the potential contribution of wind-driven dynamics to the life cycle of the eastern Indian Ocean upwelling. This paper also briefly introduces the international Indian Ocean investigation project of the SCSIO, which will advance the study of the multi-scale variability of the tropical Indian Ocean circulation system, and provide a theoretical and data basis to support marine environmental security for the countries around the Maritime Silk Road.

关键词： Equatorial undercurrent Equatorial intermediate current Eastern Indian Ocean upwelling Bay of Bengal circu lation Multi-scale variability Equatorial wave dynamics Observation and simulation