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May Chronic Childhood Constipation Cause Oxidative Stress and Potential Free Radical Damage to Children?

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Biomedical and Environmental Sciences 2004年第3期17卷 266-272页

作者： JI-YUEWANG YE-lingwang SHENG-LIZHOU JUN-FUZHOU SecondAffiliatedHospitalCollegeofMedicine ZhejiangUniversity88JiefangRoadHangzhou310009ZhejiangChina AffiliatedChildren＇sHospital CollegeofMedicineZhejiangUniversity57ZhuganLaneHangzhou310003ZhejiangChina

Objective To investigate whether chronic childhood constipation (CCC) may cause oxidative stress and potential free radical damage to children, and to explore the mechanisms by which CCC may cause oxidative stress and potential free radical damage to chronic constipation patients (CCPs). Methods Sixty CCPs and sixty healthy child volunteers (HCVs) whose ages, gender and others were matched for the CCPs were enrolled in a randomized controlled study, in which levels of vitamin C (VC) and vitamin E (VE) in plasma as well as activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes were determined by spectrophotometric analytical methods. Results Compared with average values of the above biochemical parameters in the HCVs group, the average values of VC and VE in plasma as well as those of SOD and CAT in erythrocytes in the CCPs group were significantly decreased (P<0.0001). Linear regression and bivariate correlation analysis showed that with prolonged course of the CCPs, the levels of VC and VE in plasma as well as the activities of SOD and CAT in erythrocytes in the CCPs were decreased gradually (P<0.0001). Conclusion The findings in the present study suggest that chronic childhood constipation causes oxidative stress and potential free radical damage to children with chronic constipation.

关键词： Chronic childhood constipation Oxidative stress Free radicals Free radical damage Antioxidant Antioxidase

Purification, Biochemical and Immunological Characterization of Acid Invertases from Apple Fruit

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Journal of Integrative Plant Biology 2005年第1期47卷 50-59页

作者： Qiu-HongPAN Ke-QinZOU Chang-CaoPENG Xiu-lingwang Da-PengZHANG StateKeyLaboratoryofPlantPhysiologyandBiochemistry ChinaAgriculturalUniversityBeijing100094China

: The soluble acid invertase (SAI) and cell wall-bound invertase (CWI) were purified from apple fruit to apparent electrophoretic homogeneity. Based on sequencing, substrate specificity, and immunoblotting assay, the purified enzymes were identified to be two isoforms of acid invertase (β-fructosidase; EC 3.2.1. 26). The SAI and CWI have the same apparent molecular mass with a holoenzyme of molecular mass of 220 kDa composed of 50 kDa subunits. The SAI has a lower Km value for sucrose and higher Km for raffinose compared with CWI. These acid invertases differ from those in other plants in some of their biochemical properties, such as the extremely high Km value for raffinose, no hydrolytic activity for stachyose, and a mixed form of inhibition by fructose to their activity. The antibodies directed against the SAI and CWI recognized, from the crude extract, three polypeptides with a molecular mass of 50, 68, and 30 kDa, respectively. These results provide a substantial basis for the further studies of the acid invertases in apple fruit.

关键词： antiserum cell wall-bound acid invertase fruit Malus domestica purification soluble acid invertase

Effect of Microwave and He-Ne Laser on Enzyme Activity and Biophoton Emission of Isatis indigotica Fort

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Journal of Integrative Plant Biology 2005年第7期47卷 849-855页

作者： Yi-PingCHEN Yong-JunLIU Xun-lingwang Zhao-YuREN MingYUE CollegeofEnvironmentSciences Xi＇anUniversityofArchitectureandTechnologyXi＇an710048China CollegeofLifeSciences NorthwestUniversityXi＇an710069China InstituteofPhotonicsandPhoto-Technology NorthwestUniversityXi＇an710069China DepartmentofBiotechnology Xi＇anUniversityofEngineeringScienceandTechnologyXi＇an710048China／／InstituteofEarthEnvironmenttheChineseAcademyofSciencesXi＇an710075China

Abstract: The seed embryos of Isatis indigotica Fort were exposed to He-Ne laser (5.23 mW/mm2, radiated for 5 min) and microwave (1.26 mW/mm2, radiated for 8 s) irradiation to determine the effects of microwave and He-Ne laser pretreatment on enzyme activities, and biophoton emission of cotyledon. Then: (i) changes in the activities of enzymes in I. indigotica cotyledon (such as amylase, transaminase, and proteinase) were measured to investigate the effects of He-Ne laser and microwave pretreatment; and (ii) biophoton emission was measured to determine the speed of cell division and metabolism. Results from these experiments indicated that: (i) the activities of amylase, transaminase, and proteinase of the cotyledon pretreated by He-Ne laser and microwave were significantly increased; and (ii) the intensity of biophoton emission was enhanced significantly by He-Ne laser and microwave irradiation. These changes suggest that He-Ne laser and microwave pretreatment can improve the inner energy of seeds, lead to an enhancement of cotyledon enzymes, and speed up the metabolism of the cell, resulting in significantly increased biophoton emission. Moreover, the mechanism of action of the effects of laser and microwave radiation on the microcalorimetric parameters, enzyme activities, and biophoton emission of seeds is discussed on the basis of the results obtained.

关键词： biophoton emission enzymes activities Isatis indigotica laser microcalorimetric effect microwave

Modified Multispectral Space for Color Representation

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Geo-Spatial Information Science 2005年第4期8卷 306-310页

作者： PEI Xiandeng KONG lingwang ZHU Yuanhong HUANG Hao School of Computer Science and Technology Huazhong University of Science and Technology Guanshan Wuhan 430074 China 不详

Conventional multispectral space for color representation is modified by algorithm of cluster analysis and principal component analysis. Results show that the newer modified multispectral space not only keeping the structure of the original space but eliminating zero crosses as well its accuracy of color representation is higher than the conventional.

关键词： multispectral space color representation cluster analysis principal component analysis

Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis-9,trans-11-conjugated linoleic acid

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World Journal of Gastroenterology 2002年第6期8卷 999-1004页

作者： Jia-RenLiu Bing-QingChen Yan-MeiYang Xuan-lingwang Ying-BenXue FoodScienceandToxicology DepartmentofFoodScienceComellUniversityIthacaNY14853-7201USA PublicHealthColleng HarbinMedicalUniversityHarbin150001HeilongjiangProvinceChina

AIM: To determine the effect of apoptosis on gastric cancer cells (SGC-7901) induced by cis-9, trans-11-conjugated linoleic acid (c9, t11-CLA) and its possible mechanism in the inhibition of cancer cells ***: Using cell culture, flow cytometery and immunocytochemical techniques, we examined the cell growth, frequency of apoptosis and distribution of cell cycle,expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25,50,100 and 200 μmol@L-1) of c9, t11-CLA for 24h and 48 h,with a negative control (0.1% ethanol).RESULTS: The growth of SGC-7901 cells was inhibited by c9,t11-CLA. Eight days after treatment with various concentrations of c9,t11-CLA, as mentioned above, the inhibition rates were 5.9 %, 20.2 %,75.6 % and 82.4 %, respectively. The frequency of apoptosis on SGC-7901 cells induced by different concentrations of c9, t11-CLA (except for 25 μmol@L-1, 24 h) was significantly greater than that in the negative control (P＜0.01). To further investigate the influence of the cell cycle progression, we found that apoptosis induced by c9, t11-CLA may be involved in blocking the cell cycle of SGC-7901 cells. Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations for various time periods significantly decreased the expressions of ki67 (the expression rates were 18.70-3.20 %, at 24 h and 8.10-0.20 % at 48 h, respectively), bd-2 (4.30-0.15 % at 24 h and 8.05 %-0 at 48 h),and c-myc(4.85-2.20 % at 24 h and 4.75-0.30 % at 48 h) as compared with those in the controls (the expressions of ki67, bcl-2, and c-mycwere 15.1% at 24 h and 13.5 % at 48 h, 6.80 % at 24 h and 8.00 % at 48 h,5.50 % at 24 h and 5.30 % at 48 h, respectively) (P＜0.01),whereas the expressions of Fas were increased (0.60-2.75 %,24 h and 0.45-5.95 %, 48 h).CONCLUSION: The growth and proliferation of SGC-7901 cells are inhibited by cg, t11-CLA via blocking

关键词：胃腺癌细胞系 SGC-7901 亚麻酸衍生物 cis-9 trans-11 诱发凋亡

Components of traditional Chinese medicine inhibit platelet aggregation by blocking ADP receptor subtypes P2Y1 and P2Y12

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中国药理学与毒理学杂志 2015年第S1期29卷 28-29页

作者： Xiao-liangWANG Yi-yingLI Hong-yanYANG ShaofengXU lingwang Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences Beijing 100050 China

Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular *** develop new generative,potent and safe agents for inhibiting platelet aggregation and preventing above diseases are urgently *** traditional Chinese medicines of″Houxue Huayu″have been shown to inhibit platelet aggregation *** the present study the mechanisms and the molecular targets of puerarin,salvianolic acid B and the analogue of 3-n-butylphthalide,dl-PHPB were investigated and compared with *** platelet aggregation inducers,ADP,arachidonic acid,collagen and thrombin were used in the *** was found that puerarin and dl-PHPB specifically inhibited ADP induced platelet aggregation like ticlopidine ***,salvianolic acid B inhibited both ADP and collagen induced platelet aggregations with similar *** to existing two ADP receptor subtypes on platelets,P2Y1 and P2Y12,we studied the action of above compounds on the receptors and the signaling *** was found that dl-PHPB decreased IP1 accumulation produced by ADP,but had no effect on IP1 level induced by m-3M3 FBS,an activator of PLC.M-3M3 FBS might attenuate the inhibitory effect of dl-PHPB on ADP-induced platelet *** addition,dl-PHPB did not affect cyclic AMP formation in platelets by ADP,which is different from P2Y12 antagonist *** showed the similar effects of ***,the actions of dl-PHPB and puerarin might be through P2Y1receptor-PLC-β*** acid B did not reduce the IP1 accumulation stimulated by *** might act on the receptor subtype *** results suggest that components of Chinese herb medicine might be a resource for development of novel anti-platelet drugs.

关键词： platelet aggregation ADP receptors Houxue Huayu

Migration and population genetics of the grain aphid Macrosiphum miscanti (Takahashi) in relation to the geographic distance and gene flow

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Progress in Natural Science:Materials International 2005年第11期15卷 1000-1004页

作者： GUO Wei, SHEN Zuorui, LI Zhihong and GAO lingwang (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China) College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

The population genetics of the grain aphid Macrosiphum miscanti (Takahashi) is analyzed by microsatellite markers. Samples collected from 15 locations in China have been examined at 5 polymorphic microsatellite loci. Overall, genetic diversity displays a relation between the migration and gene flow in the grain aphid; a free and frequent gene flow is found in the eastern populations, and gene isolation occurs in the two western populations, especially Datong population and Guiyang population. The natural barriers may present an insurmountable obstacle preventing gene flow and aphid migration. However, a spatial genetic differentiation between populations is correlated with their geographical separation, indicating the geographic differentiation may play an important role in shaping the genetic structure of M. miscanti populations. In addition, most populations of grain aphids are out of Hardy-Weinberg equilibrium and there is het-erozygote deficit. Based on F statistics, the average genetic differentiation among different geographical populations is relatively low. It seems that the long distance migration of the grain aphid may enhance gene flow and decrease genetic differentiation among different populations .

关键词： Macrosiphum miscanti microsatellite gene flow migration.

Comparative Analysis of Capital Productivity in China’s High-Tech Industries

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China & World Economy 2005年第2期13卷 93-105页

作者： lingwang AdamSzirmai Institute of World Economics and Politics Chinese Academy of Social ScienceChina Eindhoven Centre for Innovation Studies Eindhoventhe Netherlands.

While labor productivityis a topicof constant debate and hasbeen studied extensively, far less attention has been devoted to the question of capital productivity. Productive use of physical capital is an important source of economic growth and investment return. This paper presents a comparative study of capital productivity in China’s high-tech industry. Using a version of theperpetualinventory method (PIM), new estimates have been madeof the physical capital stock by sector. Capital productivity in China’s high-tech industry is higherthan intotalmanufacturing,butthegapbetweenthem hasbeen *** with high-tech industries in the US., highlightsthat China’shigh-tech industries could play a moreimportant rolein the growth of manufacturing and the whole economy.

关键词： capital stock productivity high-tech industries

Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B

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Progress in Natural Science:Materials International 2005年第7期15卷 665-668页

作者： ZHAO Wenran, ZHONG Xuekuan and ZHOU lingwang (Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China) Institute of Keshan Disease Chinese Research Center for Endemic Disease Control Harbin Medical University Harbin 150086 China

To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro, PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3. I/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 cells has antiviral effect against CVB3m in vitro.

关键词： PHGPx gene expression coxsackievirus group B antiviral effect.