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Eucommia ulmoides *** H_2O_2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3,6,7,and 9

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第1期12卷 47-54页

作者： Jun LIN Yi-jing FAN Christian MEHL Jia-jun ZHU Hong CHEN ling-yan jin jing-hong XU Hui-ming WANG Department of Stomatology the First Affiliated HospitaL School of Medicine Zhejiang University Hangzhou 310003 China Department of Prosthodontics Propaedeutics and Dental Materials School of Dentistry Christian-Albrechts University Kie124105 Germany Department of Plastic Surgery the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Eucommia ulmoides Oliv.(EuO),also known as Duzhong,native to China,has been reported to have antioxidative function,but its cellular mechanism is not fully examined *** investigated inhibitory effects of EuO leaf ethanol extracts on H2O2-induced apoptosis in rat osteoblastic MC3T3-E1 cells and underlying ***-grown Duzhong leaves were extracted with ***3T3-E1 cells were treated with EuO (6.25,12.5,25,50,and 100 μg/ml) for 24 h,and then H2O2 (800 μmol/L) for an additional 24 *** survival rate,percentage of apoptosis,and expressions of caspases 3,6,7,and 9 were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,microscopic analysis,Western blotting,and reverse transcription polymerase chain reaction (RT-PCR).The final EuO leaf ethanol extract powder was detected to contain caffeotannic acid at 58 mg/g and geniposide at 3.45 mg/g by high performance liquid chromatography (HPLC).EuO remarkably restrained cell oxidative damage and increased cell survival rate in a dose-dependent manner: 0 μg/ml,0.21;6.25 μg/ml,0.28;12.5 μg/ml,0.31;25 μg/ml,0.48;50 μg/ml,0.54;and 100 μg/ml,0.66 (P<0.05),with the half-effective concentration being around 25 μg/*** results were confirmed by microscopic *** blotting and RT-PCR analyses showed that the expressions of caspases 3,6,7,and 9 were significantly decreased in the EuO-treated cells compared with the control (EuOand H2O2-free) (P<0.05),with the half-effective concentration of EuO ranging from 12.5 to 25 μg/*** conclude that the ethanol-extracted EuO leaf extracts promoted the growth of MC3T3-E1 cells,and suppressed the H2O2-induced apoptosis in a rat MC3T3-E1 osteogenic cell model,likely due to the inhibition of caspases’ *** results indicate that EuO is a potent antioxidant,which may contribute to its many cellular protective functions,including the promotion of bone growth.

关键词： Eucommia ulmoides Oliv. Duzhong Oxidative damage H2O2 MC3T3-E1 cells Free radicals Apoptosis

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BDNF–TrkB signaling pathway is involved in pentylenetetrazoleevoked progression of epileptiform activity in hippocampal neurons in anesthetized rats

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Neuroscience Bulletin 2013年第5期29卷 565-575页

作者： Xu Liu Jia Liu Juan Liu Xiao-ling Liu ling-yan jin Wei Fan jin Ding Li-Chao Peng Yun Wang Xin Wang Neurology Department Zhongshan Hospital Institutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan University Department of Anaesthesia The Fifth People's Hospital of Shanghai Fudan University

Pentylenetetrazole（PTZ）is a widely-used convulsant used in studies of epilepsy;its subcutaneous injection generates an animal model with stable ***,we compared the ability of PTZ via the intravenous and subcutaneous routes to evoke progressive epileptiform activity in the hippocampal CA1 neurons of anesthetized *** involvement of the BDNF-TrkB pathway was then *** PTZ was given intravenously,it induced epileptiform bursting activity at a short latency in a dose-dependent ***,when PTZ was given subcutaneously,it induced a slowly-developing pattern of epileptogenesis;first,generating multiple population-spike peaks,then spontaneous interictal discharge-like spike,leading to the final ictal discharge-like,highly synchronized bursting firing in the CA1 pyramidal layer of the hippocampus.K252a,a TrkB receptor antagonist,when given by intracerebroventricular injection,significantly reduced the probability of multiple population spike peaks induced by subcutaneous injection of PTZ,delayed the latency of spontaneous spikes,and reduced the burst *** results indicate that PTZ induces a progressive change of neuronal epileptiform activity in the hippocampus,and the BDNF-TrkB signaling pathway is mainly involved in the early phases of epileptogenesis,but not the synchronized neuronal burst activity associated with epileptic seizure in the PTZ animal *** results provide basic insights into the changing pattern of hippocampal neuronal activity during the development of the PTZ seizure model,and establish an in vivo seizure model useful for future electrophysiological studies of epilepsy.

关键词： epilepsy pentamethylenetetrazole hippocampus BDNF TrkB

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ERK signaling mediates enhanced angiotensin II-induced rat aortic constriction following chronic intermittent hypoxia

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Chinese Medical Journal 2013年第17期126卷 3251-3258页

作者： GUO Xue-ling DENG yan SHANG jin LIU Kui XUYong-jian LIU Hui-guo Department of Respiratory and Critical Care Medicine Tongji Hospital Huazhong University of Science and Technology Key Laboratory of Respiratory Disease of the Ministry of Health Wuhan Hubei 430030 China

Background Obstructive sleep apnea （OSA） has been recognized as an independent risk factor for systemic hypertension. The study investigated the functional consequences of chronic intermittent hypoxia （CIH） on aortic constriction induced by angiotensin II （Ang II） and the possible signaling involving ERK1/2 and contractile proteins such as myosin light chain kinase （MLCK）, myosin phosphatase targeting subunit （MYPT1） and myosin light chain （MLC）. Methods Male Wistar rats were randomly divided into CIH group and normoxia group and exposed to either CIH procedure or air-air cycles. Phosphorylation of ERK1/2, MYPT1 and MLC was assessed by Western blotting following constrictor studies in the presence or absence of PD98059 （10 μmol/L）. Results CIH-exposure resulted in more body weight gain and elevated blood pressure, which could be attenuated by pretreatment with PD98059. Endothelium-removed aortic rings from CIH rats exhibited higher constrictor sensitivity to Ang II （Emax：（138.56±5.78）% versus （98.45±5.31）% of KCI; pD2：7.98±0.14 versus 8.14±0.05, respectively）. CIH procedure exerted complex effects on ERK expressions （total ERK1/2 decreased whereas the ratio of phosphorylated to total ERK1/2 increased）. CIH aortas had higher MLCK mRNA and basal phosphorylation of MYPT1 and MLC. In parallel to greater increases in phosphorylation of ERK1/2, MYPT1 and MLC, Ang II-induced aortic constriction was significantly enhanced in CIH rats, which was largely reversed by PD98059. However vascular constriction of normoxia rats remained unchanged despite similar but smaller changing tendency of proteins phosphorylation. Conclusion These data suggest that CIH exposure results in aortic hyperresponsiveness to Ang II, presumably owing to more activated ERK1/2 signaling pathway.

关键词： chronic intermittent hypoxia ERK1/2 myosin phosphatase targeting subunit myosin light chain vasoconstriction

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Ion-Velocity Map Imaging Study of Photodissociation Dynamics of Acetaldehyde

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Chinese Journal of Chemical Physics 2014年第3期27卷 249-255页

作者： Zhi-guo Zhang Zhi-chao Chen Cui-mei Zhang yan-ling jin Qun Zhang yang Chen Cun-shun Huang Xue-ming yang Hefei National Laboratory for Physical Sciences at the Microscale and Department of Chemical Physics University of Science and Technology of China Hefei 230026 China State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical Physics Dalian 116023 China

The photodissociation dynamics of acetaldehyde in the radical channel CH3＋HCO has been reinvestigated using time-sliced velocity map imaging technique in the photolysis wavelength range of 275-321 nm. The CH3 fragments have been probed via （2＋1） resonance-enhanced multiphoton ionization. Images are measured for CH3 formed in the ground and excited states （v2=0 and 1） of the umbrella vibrational mode. For acetaldehyde dissociation on T1 state after intersystem crossing from S1 state, the products are formed with high translational energy release and low internal excitation. The rotational and vibrational energy of both fragments increases with increasing photodissociation energy. The triplet barrier height is estimated at 3.8814-0.006 eV above the ground state of acetaldehyde.

关键词： Ion-velocity map imaging Photodissociation dynamics Acetaldehyde

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CsI(Tl)闪烁体探测器能量分辨率的研究

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大学物理 2017年第10期36卷 33-37页

作者：楼建玲许金艳黄学骏北京大学物理学院核物理与核技术国家重点实验室北京100871

为提高高年级学生的实验动手能力和创新能力,激发他们对实验的兴趣和热情,我们开设了研究型的教学实验:Cs I(Tl)闪烁体探测器能量分辨率的研究.该实验需要学生自己包装制作Cs I(Tl)探头,自己动手搭建测试平台并测量Cs I(Tl)探头的能量... 详细信息

为提高高年级学生的实验动手能力和创新能力,激发他们对实验的兴趣和热情,我们开设了研究型的教学实验:Cs I(Tl)闪烁体探测器能量分辨率的研究.该实验需要学生自己包装制作Cs I(Tl)探头,自己动手搭建测试平台并测量Cs I(Tl)探头的能量分辨率.整个实验最大限度地发挥了学生对实验的参与度及动手实验的机会.经过2013—2015年3年的教学实践表明,该实验是一个适合高年级本科生的研究型实验.

关键词： CsI(Tl)闪烁体能量分辨率包装材料耦合材料

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胰腺原发性去分化脂肪肉瘤:案例报道和文献综述

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世界华人消化杂志 2023年第18期31卷 791-796页

作者：杨家立金延玲孙元水浙江中医药大学第二临床医学院浙江省杭州市310053 浙江省人民医院病理科杭州医学院附属人民医院浙江省杭州市310014 浙江省立同德医院普通外科浙江省杭州市310012

背景去分化脂肪肉瘤(dedifferentiated liposarcoma,DDLPS)是一种高级别侵袭性疾病,好发于腹膜后,其次为大腿和腹股沟处,而发生在胰腺的DDLPS非常罕见.病例简介本例患者因上腹部隐痛而行CT检查提示胰腺占位性病变,性质不明.根据患者术... 详细信息

背景去分化脂肪肉瘤(dedifferentiated liposarcoma,DDLPS)是一种高级别侵袭性疾病,好发于腹膜后,其次为大腿和腹股沟处,而发生在胰腺的DDLPS非常罕见.病例简介本例患者因上腹部隐痛而行CT检查提示胰腺占位性病变,性质不明.根据患者术前病史、查体、辅助检查,考虑胰腺头颈部巨大占位性病变,边界尚清,存在手术指征,于我院接受胰十二指肠切除术,术后病理提示胰腺DDLPS,术后3 mo患者复查提示肿瘤复发,予达卡巴嗪联合表柔比星化学治疗后效果不佳,18 mo后去世.结论由于胰腺DDLPS通常对放化疗不明显,因此,目前治疗胰腺DDLPS最有效的治疗方法为显微镜下的阴性切除.但由于发生于胰腺的脂肪肉瘤位置较深,且临近周围许多器官,这使得肿瘤很难完全被切除,许多患者因此会发展为区域性复发性疾病.

关键词：胰腺脂肪肉瘤手术治疗

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古蓬种源马尾松优树子代遗传测定及选择

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西部林业科学 2023年第2期52卷 8-16页

作者：凌金燕杨章旗唐生森王玉玲胡鑫广西师范大学生命科学学院广西桂林541006 广西壮族自治区林业科学研究院广西马尾松工程技术研究中心/广西优良用材林资源培育重点实验室广西南宁530000 广西百色市百林林场广西百色533000

为探索古蓬种源马尾松优树子代生长性状变异规律,筛选品系优良的家系和单株,为古蓬种源马尾松天然群体保护和遗传改良提供参考。以54个古蓬种源马尾松优树子代半同胞家系为材料,通过对4 a生和8 a生马尾松的生长性状变异分析和遗传参数估... 详细信息

为探索古蓬种源马尾松优树子代生长性状变异规律,筛选品系优良的家系和单株,为古蓬种源马尾松天然群体保护和遗传改良提供参考。以54个古蓬种源马尾松优树子代半同胞家系为材料,通过对4 a生和8 a生马尾松的生长性状变异分析和遗传参数估算,并基于主成分估计法的因子分析、聚类分析与遗传增益相结合筛选优良家系及单株。结果显示:(1)4 a生和8 a生马尾松家系间胸径、树高、单株材积分化明显且均存在显著差异(P<0.05);遗传参数估算发现4 a生时马尾松半同胞家系受低等遗传力控制,8 a生时则为中等水平。(2)4 a生和8 a生两个年龄的马尾松分别入选的优良家系14个和11个,均入选的优良家系4个,入选的优良家系各性状(胸径、树高和单株材积)的平均遗传增益4 a生分别为0.95%、1.04%和3.57%,8 a生时为4.06%、1.91%和10.17%,8 a生更宜作为马尾松优良家系选择林龄;8 a生时从11个优良家系中筛选出26株优良单株,其胸径、树高和单株材积平均现实增益分别为46.24%、26.34%和137.50%,平均遗传增益分别为11.44%、5.15%和33.49%,选择效果显著。结果表明:古蓬种源马尾松生长性状家系间具有很大的选育潜力,所选出的优良家系及单株可为古蓬种源马尾松天然种群的保护和遗传改良提供极佳的种质资料。

关键词：马尾松古蓬种源子代测定遗传增益因子分析

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A prospective randomized antiplatelet trial of cilostazol versus clopidogrel in patients with bare metal stent

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Chinese Medical Journal 2006年第5期119卷 360-366页

作者： CHEN Yun-dai, LU yan-ling, jin Ze-ning, YUAN Fei and Lü Shu-zhengDepartment of Cardiology, Beijing Anzhen Hospital, Capital University of Medical Sciences Beijing Institute of Heart, Lung and Blood Vessel, Beijing 100029, China Department of Cardiology Beijing Anzhen Hospital Capital University of Medical Sciences

Background Cilostazol is a newly developed antiplatelet drug that has been widely applied for clinical use. Its antiplatelet action appears to be mainly related to inhibition of intracellular phosphodiesterase activity. Recently, cilostazol has been used for antiplatelet therapy after coronary bare metal stent implantation for thrombosis and restenosis prevention. This prospective randomized and double blind trial was designed to investigate the safety and efficacy of cilostazol for the prevention of late restenosis and acute or subacute stent thrombosis. Methods One hundred and twenty patients who underwent elective stent were randomly assigned to treatment group with cilostazol 200 mg/d （n = 60）, clopidogrel 75 mg/d and aspirin 100 mg/d or to control group with clopidogrel treatment 75 mg/d （n = 60） and aspirin 100 mg/d. Follow-up coronary angiography was performed 6--9 months later. Results Nine months major adverse cardio-cerebral event （MACCE） were lower in treatment groups （P〈0.05）. The quantitative coronary angiography （QCA） at 6 months follow-up showed that minimum lumen diameter （MLD） was higher in treatment group than that of control group [（2.14 ±　0.52）mm vs （1.82 ± 0.36）mm, P〈0.05]. Late lumen loss （LL） [（0.82 ± 0.42）mm vs （1.31 ± 0.58）mm; P〈0.01 ], restenosis rate （RR）（14% vs 32%; P〈0.05） and target lesion revascularizaion （TLR） rate （5% vs 17%; P〈0.05） were lower in treatment group than in control group. Conclusion Cilostazol therapy is an effective regimen for prevention not only stent thrombosis but also RR and TLR through reducing MLD without the risk of increasing bleeding.

关键词： cilostazol clopidogrel restenosis platelet aggregation inhibitors

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Copper（Ⅱ）-assisted DNA-cleaving activities of berberine carboxylate

Copper（Ⅱ）-assisted DNA-cleaving activities of berberine c...

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2011年全国药物化学学术会议——药物的源头创新

作者： Wei-Er Lin,Chun-Qiong Zhou,Ming-Zhen Chen,yan-ling Lin,jin-Xiang Chen~*,and Wen-Hua Chen~* School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515.

＜正＞Many biologically active molecules function in the living cells by recognizing,modifying, cleaving or crosslinking ***,considerable attentions have been paid to synthetic agents,in particular the ones that are capable of recognizing and cleaving ***,we describe the synthesis of berberine carboxylate 1（Figure 1（a）） in which the berberine unit serves

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镉对大肠杆菌生长代谢作用的微量热研究

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武汉理工大学学报 2005年第1期27卷 19-21页

作者：李曦丁玲靳艳巧武汉理工大学理学院武汉430070

以大肠杆菌为模型生物,用微量热法研究了镉对大肠杆菌生长代谢作用的影响,并与比浊法进行了比较。实验表明2种方法结果相近,镉可抑制大肠杆菌的生长,而且镉的浓度越高,对大肠杆菌生长代谢的抑制作用越强。微量热法较传统的比浊法具有独... 详细信息

以大肠杆菌为模型生物,用微量热法研究了镉对大肠杆菌生长代谢作用的影响,并与比浊法进行了比较。实验表明2种方法结果相近,镉可抑制大肠杆菌的生长,而且镉的浓度越高,对大肠杆菌生长代谢的抑制作用越强。微量热法较传统的比浊法具有独到之处,对于了解镉的生物效应及其对细胞作用的生理特征具有重要的意义。

关键词：微量热法比浊法镉大肠杆菌生长代谢

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