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Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis

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Frontiers of Medicine 2024年第1期18卷 147-168页

作者： Weiwei Xian Mohammad Asad Shuai Wu Zhixin Bai Fengjiao Li Junfeng Lu Gaoyu zu Erin Brintnell Hong Chen Ying Mao Guomin Zhou Bo Liao Jinsong Wu Edwin Wang Linya You Department of Human Anatomy&Histoembryology School of Basic Medical SciencesFudan UniversityShanghai200032China Cumming School of Medicine University of CalgaryCalgaryAlbertaT2N 4N1Canada Glioma Surgery Division Department of NeurosurgeryHuashan HospitalFudan UniversityShanghai200040China Department of Pathology Huashan HospitalFudan UniversityShanghai200040China Department of Neurosurgery Huashan HospitalFudan UniversityShanghai200040China Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention Fudan UniversityShanghai200040China School of Mathematics and Statistics Hainan Normal UniversityHaikou570100China

The association of neurogenesis and gliogenesis with glioma remains *** conducting single-cell RNA-seq analyses on 26 gliomas,we reported their classification into primitive oligodendrocyte precursor cell(pri-OPC)-like and radial glia(RG)-like tumors and validated it in a public cohort and TCGA *** RG-like tumors exhibited wild-type isocitrate dehydrogenase and tended to carry EGFR mutations,and the pri-OPC-like ones were prone to carrying TP53 *** subclones only in pri-OPC-like tumors showed substantially down-regulated MHC-I genes,suggesting their distinct immune evasion ***,the two subgroups appeared to extensively modulate glioma-infiltrating lymphocytes in distinct *** specific genes not expressed in normal immune cells were found in glioma-infiltrating *** example,glial/glioma stem cell markers OLIG1/PTPRZ1 and B cell-specific receptors IGLC2/IGKC were expressed in pri-OPC-like and RG-like glioma-infiltrating lymphocytes,*** expression was positively correlated with those of immune checkpoint genes(e.g.,LGALS33)and poor survivals as validated by the increased expression of LGALS3 upon IGKC overexpression in Jurkat *** finding indicated a potential inhibitory role in tumor-infiltrating lymphocytes and could provide a new way of cancer immune evasion.

关键词： single-cell RNA-seq glioma radial glia primitive oligodendrocyte precursor cell immune escape

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Washed microbiota transplantation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabolomic-based study

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World Journal of Gastroenterology 2024年第11期30卷 1572-1587页

作者： Shi-Ju Chen Da-Ya Zhang Xia Wu Fa-Ming Zhang Bo-Ta Cui Yi-Hao Huang zu-Lun Zhang Rui Wang Fei-Hu Bai Graduate School Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Microbiota Medicine&Medical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of Gastroenterology The Second Affiliated Hospital of Hainan Medical UniversityHaikou 570216Hainan ProvinceChina

BACKGROUND Fecal microbiota transplantation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transplantation(WMT).Most existing studies have focused on observing the clinical ***,the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome,metatranscriptome,and metabolome-has not yet been *** To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to *** We conducted a prospective,open-label,single-center clinical *** CD patients underwent *** clinical responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabolome profiles were evaluated three months after the *** Seven of the 11 patients(63.6%)showed an optimal clinical response three months *** microbiome diversity significantly increased after WMT,consistent with improved clinical *** of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains,such as Faecalibac-terium prausnitzii,Roseburia intestinalis,and Escherichia *** addition,metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment compared to the ***,levels of vital amino acids that may be associated with disease progression(e.g.,L-glutamic acid,gamma-glutamyl-leucine,and prolyl-glutamine)were reduced after *** WMT demonstrated therapeutic efficacy in CD treatment,likely due to the effective reconstruction of the patient’s ***-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

关键词： Crohn’s disease Clinical trials Fecal microbiota transplant Metagenome Metatranscriptome Metabolome

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创新水平视角下数字经济对环境污染的影响——以长江经济带40个沿线城市为例

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长江流域资源与环境 2024年第1期33卷 126-138页

作者：陈祖海符斌中南民族大学经济学院湖北武汉430074 中国武陵山乡村发展研究院湖北武汉430074

数字经济发展迅速,探究数字经济对环境污染的影响和作用机制,对于推动长江经济带高质量发展具有重要意义。基于2011~2019年长江经济带沿线40个地级及以上城市的面板数据,探讨创新水平在数字经济与环境污染之间的作用机制,利用门槛效应... 详细信息

数字经济发展迅速,探究数字经济对环境污染的影响和作用机制,对于推动长江经济带高质量发展具有重要意义。基于2011~2019年长江经济带沿线40个地级及以上城市的面板数据,探讨创新水平在数字经济与环境污染之间的作用机制,利用门槛效应模型分析其非线性关系及作用区间,进一步借助空间杜宾模型考察数字经济产生的空间溢出效应。结果表明:(1)数字经济对于本地的环境污染具有显著的抑制作用,且经过多种稳健性检验,结论依然成立。(2)创新水平是数字经济影响环境污染的重要路径,且当创新水平超过门槛值6.41后,数字经济对于环境污染的影响系数将从-0.024变为-0.042,存在边际递增的治污效果。(3)在抑制本地环境污染的同时数字经济的空间溢出效应会加剧周边地区的污染排放;产量规模的扩张可能成为污染加剧的原因,数字经济对环境污染的抑制效果被生产规模扩大带来的污染增长所覆盖;为此,需要加强地区间协同治理,避免生产规模的不合理稀释数字经济的治污效果。

关键词：数字经济创新水平环境污染门槛效应空间溢出长江经济带

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张奠宙数学教育思想概述

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数学教育学报 2024年第3期33卷 89-95页

作者：刘祖希华东师范大学出版社上海200062

张奠宙是中国著名数学教育家,他的数学教育思想值得研究与传承.张奠宙数学教育思想包括以下7个方面:数学的学术形态与教育形态思想;数学文化就是要“文而化之”的思想;数学美与数学欣赏的思想;重视数学应用的思想;数学史融入数学教育的... 详细信息

张奠宙是中国著名数学教育家,他的数学教育思想值得研究与传承.张奠宙数学教育思想包括以下7个方面:数学的学术形态与教育形态思想;数学文化就是要“文而化之”的思想;数学美与数学欣赏的思想;重视数学应用的思想;数学史融入数学教育的思想;数学“双基”“四基”教学模型的思想;数学教育的“中国道路”思想.7个方面构成知行合一、教学相长、辩证统一的有机整体.

关键词：张奠宙数学的教育形态数学文化数学美数学应用数学史双基中国道路

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基于broccoli的RNA荧光适体设计与筛选

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四川大学学报（自然科学版） 2024年第4期61卷 310-319页

作者：何祖洋白九元赵云四川大学生命科学学院生物资源与生态环境教育部重点实验室成都610065

为了在活细胞RNA成像中提供更加精准的工具,本文旨在基于broccoli的RNA荧光标记创建荧光强度高、稳定性好的绿色荧光适体.采用理性设计对broccoli进行优化,并通过测定光谱特征和理化性质对broccoli变体进行多轮筛选.结果发现基于对RNA... 详细信息

为了在活细胞RNA成像中提供更加精准的工具,本文旨在基于broccoli的RNA荧光标记创建荧光强度高、稳定性好的绿色荧光适体.采用理性设计对broccoli进行优化,并通过测定光谱特征和理化性质对broccoli变体进行多轮筛选.结果发现基于对RNA荧光适配体broccoli的三轮突变筛选,获得了荧光强度提升3.3倍的broccoli四位点突变体(命名为Bro);再利用设计的3WJ支架,组建了3WJ-n×Bro RNA荧光适配体家族;采用嵌入串联重复Bro的方式可以显著提升适体的荧光强度,其中3WJ-4×Bro的荧光强度超过3WJ-Bro的2.7倍,且表现出更低的盐离子依赖性和更高的热稳定性、光稳定性和监测灵敏度.结果表明本文设计出的3WJ-n×Bro家族并从中筛选出优异的核酸适配体3WJ-4×Bro,可为在RNA水平上实现活细胞RNA成像提供可视化工具.

关键词： RNA荧光适体 Broccoli适体 RNA成像

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信江底泥重金属污染异位修复处理技术

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科学技术与工程 2024年第21期24卷 9244-9251页

作者：莫子奋祖凌鑫沈应龙江西地质局第六地质大队南昌330000

为整治城市河道底泥重金属污染,以江西鹰潭信江河道污染底泥为处理对象。采用化学淋洗和固化稳定化技术相结合治理河道底泥重金属污染,设计化学淋洗模型,分析淋洗效果和固化稳定化参数。结果表明,理想淋洗剂为草酸,淋洗时间8 h,固液比1... 详细信息

为整治城市河道底泥重金属污染,以江西鹰潭信江河道污染底泥为处理对象。采用化学淋洗和固化稳定化技术相结合治理河道底泥重金属污染,设计化学淋洗模型,分析淋洗效果和固化稳定化参数。结果表明,理想淋洗剂为草酸,淋洗时间8 h,固液比1∶5,浓度0.2 mol/L。复合稳定剂最优配比为FeSO_(4)∶MgO∶高岭土=9∶11∶6,投加量为100%。固化材料配比选择底泥∶水泥∶石灰∶砂子=50∶6∶4∶5。处理后的底泥浸出重金属浓度低于标准限值,固化后无侧限抗压强度达到3.48 MPa。

关键词：河道底泥重金属化学淋洗固化稳定化

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Theoretical Research on a D-band Traveling Wave Extended Interaction Amplifier

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Chinese Journal of Electronics 2024年

作者： Zhongtao CUI Xuesong YUAN Xiaotao XU Dongrui CHEN Yifan zu Matthew Thomas Cole Qingyun CHEN Yang YAN Terahertz Science and Technology Key Laboratory of Sichuan Province School of Electronic Science and EngineeringUniversity of Electronic Science and Technology of China Department of Electronic and Electrical Engineering University of Bath

A traveling-wave, extended interaction amplifier is herein investigated for use in millimeter-wave and terahertz amplification sources. By placing engineered extended interaction cavities between the traveling wave structures, higher gain is obtained with a shorter high frequency circuit, compared with conventional traveling wave tubes architectures. The bandwidth of the device is significantly increased relative to extended interaction klystrons. A Dband beam wave interaction circuit of 26 mm long has been designed. Particle in cell simulations at 21.5 kV operating voltage, 0.3 A beam current, and 5 mW input power show that the maximum output power reaches 351 W, with a gain of 48.4 dB and 3-dB bandwidth of 1.42 GHz.

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基于CIPP评价模式的中医专业床边教学质量评价体系的构建与探索

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创新创业理论研究与实践 2024年第10期7卷 5-9+29页

作者：杨杰祖强南京中医药大学

中医专业床边教学在培养学生临床实践能力和医学素养方面起着重要作用。然而，当前对于中医专业床边教学的评价体系仍然不完善，缺乏相应的指标和方法。该文基于CIPP评价模式，通过分析中医专业床边教学的特点和需求，构建了床边教学质... 详细信息

中医专业床边教学在培养学生临床实践能力和医学素养方面起着重要作用。然而，当前对于中医专业床边教学的评价体系仍然不完善，缺乏相应的指标和方法。该文基于CIPP评价模式，通过分析中医专业床边教学的特点和需求，构建了床边教学质量评价体系，并对其进行了探索和验证。实践结果表明：该评价体系可以有效地评估中医专业床边教学的质量，为教学改进和学生学习提供指导和支持。

关键词： CIPP评价模式中医专业床边教学质量评价体系构建评估指标

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The association and underlying mechanism of the digit ratio(2D:4D)in hypospadias

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Asian Journal of Andrology 2024年第4期26卷 356-365页

作者： Yu Ding zu-Quan Chen Wen-Feng Pan Hao-Jie Chen Min Wu Yi-Qing Lyu Hua Xie Yi-Chen Huang Zhong-Zhong Chen Fang Chen Department of Urology Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200062China Urogenital Development Research Center Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200062China Clinical Research Center for Hypospadias Pediatric CollegeShanghai Jiao Tong University School of MedicineShanghai 200062China.

The second-to-fourth digit(2D:4D)ratio is thought to be associated with prenatal androgen ***,the relationship between the 2D:4D ratio and hypospadias is poorly understood,and its molecular mechanism is not *** this study,by analyzing the hand digit length of 142 boys with hypospadias(23 distal,68 middle,and 51 proximal)and 196 controls enrolled in Shanghai Children’s Hospital(Shanghai,China)from December 2020 to December 2021,we found that the 2D:4D ratio was significantly increased in boys with hypospadias(P<0.001)and it was positively correlated with the severity of the *** was further verified by the comparison of control mice and prenatal low testosterone mice model obtained by knocking out the risk gene(dynein axonemal heavy chain 8[DNAH8])associated with ***,the discrepancy was mainly caused by a shift in *** characterization of a mouse model validated that low testosterone levels during pregnancy can impair the growth and development of *** mechanistic explorations revealed that during the androgen-sensitive window,the downregulation of the androgen receptor(AR)caused by low testosterone levels,as well as the suppressed expression of chondrocyte proliferation-related genes such as Wnt family member 5a(Wnt5a),Wnt5b,Smad family member 2(Smad2),and Smad3;mitochondrial function-related genes in cartilage such as AMP-activated protein kinase(AMPK)and nuclear respiratory factor 1(Nrf-1);and vascular development-related genes such as myosin light chain(MLC),notch receptor 3(Notch3),and sphingosine kinase 1(Sphk1),are responsible for the limitation of 4D growth,which results in a higher 2D:4D ratio in boys with hypospadias via decreased endochondral *** study indicates that the ratio of 2D:4D is a risk marker of hypospadias and provides a potential molecular mechanism.

关键词： androgen receptor digit ratio(2D:4D) endochondral ossification hypospadias testosterone level

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Broadband bidirectional Brillouin–Raman random fiber laser with ultra-narrow linewidth

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Chinese Physics B 2024年第2期33卷 371-376页

作者：杨茜李阳邹辉梅杰徐恩明张祖兴 Advanced Photonic Technology Laboratory College of Electronic and Optical Engineering&College of MicroelectronicsNanjing University of Posts and TelecommunicationsNanjing 210023China

We present a Brillouin–Raman random fiber laser(BRRFL)with full-open linear cavity structure to generate broadband Brillouin frequency comb(BFC)with double Brillouin-frequency-shift *** incorporation of a regeneration portion consisting of an erbium-doped fiber and a single-mode fiber enables the generation of broadband *** dynamics of broadband BFC generation changing with the pump power(EDF and Raman)and Brillouin pump(BP)wavelength are investigated in detail,*** suitable conditions,the bidirectional BRRFL proposed can produce a flatamplitude BFC with 40.7-nm bandwidth ranging from 1531 nm to 1571.7 nm,and built-in 242-order Brillouin Stokes lines(BSLs)with double Brillouin-frequency-shift ***,the linewidth of single BSL is experimentally measured to be about 2.5 *** broadband bidirectional narrow-linewidth BRRFL has great potential applications in optical communication,optical sensing,spectral measurement,and so on.

关键词： random laser fiber laser stimulated Brillouin scattering(SBS) stimulated Raman scattering(SRS)

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