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Chinese Journal of Natural Medicines 2017年第3期15卷 234-240页

作者： CHEN Qian-Qian XI Hai-ling WANG Cai-yun XU Feng-guo ZHANG Wei State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and Technology School of Public Health Baotou Medical College Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education) China Pharmaceutical University

The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 *** extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C_(18) column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring *** mobile phase was consisted of methanol-water(0.1% formic acid)(55:45,V/V).The total run time was 5.0 *** method was linear in the concentration range of 0.25-250.0 ng·mL^(-1).The lower limit of quantification was 0.25 ng·mL^(-1).The intra-and inter-day relative standard deviations of entire concentration range were less than 9.3%.The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.

关键词： Camellianin A HepG2 cells HPLC-MS/MS

High-throughput genotyping of single-nucleotide polymorphisms in ace-1 gene of mosquitoes using MALDI-TOF mass spectrometry

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Insect Science 2013年第2期20卷 167-174页

作者： yun Mao Feng Tan Shuai-guo Yan guo-Xing Wu Chuan-ling Qiao Wen-Xue Zhang Feng Cui College of Life Sciences Henan Normal University Xinxiang Henan Province State Key Laboratory of Integrated Management of Pest Insects & Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Plant Protection Yunnan Agricultural University Kunming China

Acetylcholinesterase （ACHE） plays a vital role in the nervous system of insects and other animal species and serves as the target for many chemical agents such as organophosphate and carbamate insecticides. The mosquito, Culex pipiens complex, a vector of human disease, has evolved to be resistant to insecticides by a limited number of amino acid substitutions in ACHE1, which is encoded by the ace-1 gene. The aims of this study are to identify single nucleotide polymorphism （SNP） sites in the ace-1 gene of the C. pipiens complex and explore an economical high-throughput method to differentiate the genotypes of these sites in mosquitoes collected in the field. We identified 22 SNP sites in exon regions of the ace-1 gene. Four of them led to non-synonymous mutations, that is, Y163C, G247S, C677S and T682A. We used matrix-assisted laser desorption ionization - time-of-flight mass spectrometry for genotyping at these four sites and another site F416V, which was relevant to insecticide resistance, in 150 mosquitoes collected from 15 field populations. We were able to synchronize analysis of the five SNP sites in each well of a 384-well plate for each individual mosquito, thus decreasing the cost to one-fifth of the routine analysis. Heterozygous genotypes at Y163C and G247S sites were observed in one mosquito. The possible influence of the five SNP sites on the activity or function of the enzyme is discussed based on the predicted tertiary structure of the enzyme.

关键词： acetylcholinesterase genotype insecticide resistance MassArray SNP

AN EXPANDED CHARACTERISTIC-MIXED FINITE ELEMENT METHOD FOR A CONVECTION-DOMINATED TRANSPORT PROBLEM

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Journal of Computational Mathematics 2005年第5期23卷 479-490页

作者： ling guo Huan-zhen Chen Department of Mathematics Shanghai Jiao Tong UniversityShanghai 200030China Department of Mathematics Shandong Normal UniversityJinan 250014China

In this paper, we propose an Expanded Characteristic-mixed Finite Element Method for approximating the solution to a convection dominated transport problem. The method is a combination of characteristic approximation to handle the convection part in time and an expanded mixed finite element spatial approximation to deal with the diffusion part. The scheme is stable since fluid is transported along the approximate characteristics on the discrete level. At the same time it expands the standard mixed finite element method in the sense that three variables are explicitly treated： the scalar unknown, its gradient, and its flux. Our analysis shows the method approximates the scalar unknown, its gradient, and its flux optimally and simultaneously. We also show this scheme has much smaller time-truncation errors than those of standard methods. A numerical example is presented to show that the scheme is of high performance.

关键词： Convection diffusion problems Expanded characteristic mixed finite elementmethod Optimal error estimates Numerical test

Vertebral body infection due to esophageal perforation caused by ingestion of fishbone:a case report

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Frontiers of Nursing 2020年第2期7卷 77-79页

作者： ling-yun Zhang Hong Liang Xue-guo Sun Department of Gastroenterology The Affiliated Hospital of Qingdao UniversityQingdaoShandong 266000China The Community Healthcare Center of Yan’an’er Road QingdaoShandong 266000China

Background:Esophageal injury is a common complication of foreign bodies in the upper gastrointestinal tract,but bilateral pleural effusion or vertebral infection is a rare condition due to a swallowed *** is considerably difficult for a physician to diagnose quickly because of incomplete patient history of foreign bodies ingestion and/or insufficient *** concerns:We describe the case of a 56-year-old man who was admitted to an emergency medical department owing to a low to moderate fever for 7 *** a series of examinations,the patient was diagnosed with esophageal perforation(EP)caused by a fishbone that was swallowed half a month ***:About 12 days after the onset of fever,he was diagnosed with EP based on the gastric endoscopic images combined with histological section and sufficient history of the *** 2 months later,the patient has obvious back pain and lack of strength in two legs and was diagnosed with vertebral body ***:Antibiotic therapy,multi-disciplinary team(MDT),and surgical intervention had been ***:It is very fortunate for this patient to have a good prognosis due to a timely diagnosis and proper *** power has attained level ***:Several lessons can be learned from this case;for example,physicians should be alerted to the EP,endoscopic intervention should be prompt,antibiotics should be used regularly,and so on.

关键词： esophageal perforation spinal infection foreign body operation

Observer-based multivariable fixed-time formation control of mobile robots

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Journal of Systems Engineering and Electronics 2020年第2期31卷 403-414页

作者： LI Yandong ZHU ling and guo Yuan College of Computer and Control Engineering Qiqihar UniversityQiqihar 161006China School of Mechanical and Electronic Engineering Qiqihar UniversityQiqihar 161006China

This paper proposes a multivariable fixed-time leaderfollower formation control method for a group of nonholonomic mobile robots, which has the ability to estimate multiple uncertainties. Firstly, based on the state space model of the leader-follower formation, a multivariable fixed-time formation kinematics controller is designed. Secondly, to overcome uncertainties existing in the nonholonomic mobile robot system, such as load change,friction, external disturbance, a multivariable fixed-time torque controller based on the fixed-time disturbance observer at the dynamic level is designed. The designed torque controller is cascaded with the formation controller and finally realizes accurate estimation of the uncertain part of the system, the follower tracking of reference velocity and the desired formation of the leader and the follower in a fixed-time. The fixed-time upper bound is completely determined by the controller parameters, which is independent of the initial state of the system. The multivariable fixed-time control theory and the Lyapunov method are adopted to ensure the system ***, the effectiveness of the proposed algorithm is verified by the experimental simulation.

关键词： multivariable fixed-time control formation control uncertainty fixed time observer nonholonomic mobile robot

Methodology development and application of X-ray imaging beamline at SSRF

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Nuclear Science and Techniques 2020年第10期31卷 74-94页

作者： Hong-Lan Xie Biao Deng guo-Hao Du Ya-Nan Fu Han guo Yan-ling Xue Guan-yun Peng Fen Tao ling Zhang Ti-Qiao Xiao Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Shanghai Synchrotron Radiation Facility(SSRF) Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China

This paper introduces some latest developments regarding the X-ray imaging methodology and applications of the X-ray imaging and biomedical application beamline(BL13W1)at Shanghai Synchrotron Radiation Facility in the past 5 *** photon energy range of the beamline is 8–72.5 *** sets of X-ray imaging detectors with different pixel sizes(0.19–24 lm)are used to realize X-ray microcomputed tomography(X-ray micro-CT)and X-ray in-line phase-contrast *** satisfy the requirements of user experiments,new X-ray imaging methods and image processing techniques are *** vivo dynamic micro-CT experiments with living insects are performed in 0.5 s(sampling rate of 2 Hz,2 tomograms/s)with a monochromatic beam from a wiggler source and in 40 ms(sampling rate of 25 Hz,25 tomograms/s)with a white beam from a bending magnet source.A new X-ray imaging method known as move contrast X-ray imaging is proposed,with which blood flow and moving tissues in raw images can be distinguished according to their moving frequencies in the time ***,X-ray speckle-tracking imaging with twice exposures to eliminate the edge enhancement effect is developed.A high-precision quantification method is realized to measure complex three-dimensional blood vessels obtained via X-ray micro-CT.X-ray imaging methods such as three-dimensional X-ray diffraction microscopy,small-angle X-ray scattering CT,and X-ray fluorescence CT are developed,in which the X-ray micro-CT imaging method is combined with other contrast mechanisms such as diffraction,scattering,and fluorescence contrasts ***,an X-ray nano-CT experiment is performed with a 100 nm spatial *** user experimental results from the fields of material science,biomedicine,paleontology,physics,chemistry,and environmental science obtained on the beamline are provided.

关键词： X-ray imaging X-ray in-line phase-contrast imaging X-ray micro-CT Dynamic micro-CT X-ray speckle-tracking imaging 3DXRD SAXS-CT X-ray fluorescence CT X-ray nano-CT Move contrast X-ray imaging

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li Weiwei guo Shuolong Yuan Liangwei Xu Chang Lin Xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

MoS2 synaptic transistor with one-step manufacture

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Science China(Information Sciences) 2025年第1期68卷 357-365页

作者： Yihao guo Yang WANG Wenjie DENG Yi WU Jingtao LI Kexin LI Yuehui ZHAO Songlin YU Xiaoting WANG Yongzhe ZHANG Hui YAN Key Laboratory of Optoelectronics Technology of Education Ministry of China Faculty of Information TechnologyBeijing University of Technology Key Laboratory of Advanced Functional Materials Ministry of Education Faculty of Materials and ManufacturingBeijing University of Technology The 11th Research Institute of Electronics Technology Group Corporation

The synaptic transistor,which is a neuromorphic device with brain-like functions,has garnered significant attention due to characteristics such as low energy consumption,high integration,multiple functions,and high computational ***,the current synaptic transistor possesses a complicated structure and involves complex manufacturing steps,which is not conducive to low-cost,large-scale bionic ***,we introduce a simple MoS2synaptic transistor exhibiting robust synaptic *** transistor utilizes magnetron sputtering manufacture to combine the process of device metal electrode deposition and the introduction of defect states,which helps streamline the manufacturing process into a single *** long-term potentiation and long-term depression in photoelectric coordination are initially ***,biological functions,such as double pulse facilitation and the transformation behavior of short-term memory to long-term memory,are thoroughly evaluated,along with learning-forgetting-relearning ***,the device exhibits robust synaptic plasticity until incident light angles down to 30° to imitate the multi-to-one axon-cellular ***,a classical simulation of Pavlov's dog conditioned reflex is demonstrated along with the multiview extraction *** experimental results demonstrate a promising synaptic transistor with a simple structure,manufacturing technique,and excellent *** further indicates the potential for large-scale integration of bionic neuromorphic systems.

关键词： process optimization artificial synaptic device one-stepmanufacture synaptic plasticity bionic simulation

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Recent Progress in Donor-Acceptor Type Conjugated Polymers for Organic Field-effect Transistors

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Chinese Journal of Polymer Science 2023年第5期41卷 652-670,I0006页

作者： Yi-Fan Li yun-Long guo yun-Qi Liu Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic solidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

The recent progress in the design and synthesis of high-performance donor-acceptor conjugated polymeric semiconducting materials is reviewed from the perspective of multiscale *** multiscale of conjugated polymers is from the primary one-dimensional polymer molecular scale to the secondary polymer-chains interaction scale,and then to the tertiary polymer aggregate *** review focuses on the design and synthesis of polymer molecules,proposes new classification rules,and rationally summarizes the design strategies and modulation methods of *** describe the recent progress from these three aspects:(1)the modification ofπ-conjugated backbone,(2)the evolution of the polymerization methods,and(3)the regulation of aggregate-state structure.

关键词： Conjugated polymers Donor-acceptor Organic field-effect transistors Multiscale optimization

Study on serum cytokine levels in posttraumatic stress disorder patients

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Asian Pacific Journal of Tropical Medicine 2012年第4期5卷 323-325页

作者： Min guo Tao Liu Jun-Cheng guo Xiang-ling Jiang Feng Chen yun-Suo Gao People's Hospital of Hainan Province Hainan Medical College

Objective:To evaluate serum IL-2,IL-4,***-8,TL-10 and TNF-αlevels in posttraumatic stress disorder(PTSD) ***:We utilized ELISA technology to examine cytokines such as IL-2,***-8,IL-10 and TNF-αin serum from 50 well-characterized individuals with a primary DSM-1V PTSD diagnosis,and 50 age- and gender-matched healthy *** conservatively employed a Mann-Whitney U ***:Individuals with primary PTSD had significantly elevated peripheral cytokine levels for all 6 different cytokines compared to age- and gender-matched healthy controls(allP<0.01).Conclusions:These findings suggest that a generalized inflammatory state may be present in individuals with PTSD.

关键词： Cytokines Posttraumatic stress disorder