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Scutellaria barbata attenuates diabetic retinopathy by preventing retinal inflammation and the decreased expression of tight junction protein

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 870-877页

作者： Xi-yu Mei ling-yu zhou Tian-yu Zhang Bin Lu Li-Li Ji The MOE Key Laboratory for Standardization of Chinese Medicines Shanghai Key Laboratory of Complex Prescription Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Shanghai 201203 China

AIM： To observe the attenuation of ethanol extract of Herba Scutellaria barbata （SE） against diabetic retinopathy （DR） and its engaged mechanism. METHODS： C57BL/6J mice were intraperitoneally injected with streptozotocin （STZ, 55 mg/kg） for 5 consecutive days to induce diabetes, The diabetic mice were orally given with SE （100, 200 mg/kg） for lmo at lmo after STZ injection. Blood-retinal barrier （BRB） breakdown was detected by using Evans blue permeation assay. Real-time polymerase chain reaction （RT-PCR）, Western blot and immunofiuorescence staining were used to detect mRNA and protein expression. Enzyme-linked immunosorbent assay （ELISA） was used to detect serum contents of tumor necrosis factor-e （TNF-a） and interleukin （IL）-II. RESULTS： SE （100, 200 mg/kg） reversed the breakdown of BRB in STZ-induced diabetic mice. The decreased expression of retinal claudin-1 and claudin-19, which are both tight junction （T J） proteins, was reversed by SE. SE decreased the increased serum contents and retinal mRNA expression of TNF-a and IL-113. SE also decreased the increased retinal expression of intercellular cell adhesion molecule-1 （ICAM-1）. SE reduced the increased phosphorylation of nuclear factor kappa B （NFKB） p65 and its subsequent nuclear translocation in retinas from STZ- induced diabetic mice. Results of Western blot and retinal immunofluorescence staining of ionized calcium-binding adapter molecule 1 （Ibal） demonstrated that SE abrogated the activation of microglia cells in STZ-induced diabetic mice. CONCLUSION： SE attenuates the development of DR by inhibiting retinal inflammation and restoring the decreased expression of TJ proteins including claudin-1 and claudin-19.

关键词： scutellaria barbata diabetic retinopathy tightjunction inflammation nuclear factor kappa B microglia

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CYP27A1 mutation in a case of cerebrotendinous xanthomatosis:A case report

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World Journal of Clinical Cases 2022年第18期10卷 6168-6174页

作者： Zhao-Ran Li yu-ling zhou Qi Jin Yin-Yin Xie Hong-Mei Meng Department of Neurology The First Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND Cerebrotendinous xanthomatosis(CTX)is a rare autosomal recessive metabolic disease caused by mutations in CYP27 *** has a low incidence rate,insidious onset,and diverse clinical *** can be easily misdiagnosed and can go unrecognized by clinicians,leading to delayed treatment and worsened patient *** SUMMARY A 38-year-old male was admitted to our hospital with a history of unabating unstable posture and difficulty in walking for more than 30 *** based on the patient’s medical history,clinical symptoms,magnetic resonance imaging and gene sequencing results,he was finally diagnosed with *** to the low incidence rate of the disease,clinicians have insufficient knowledge of it,which makes the diagnosis process more tortuous and prolongs the diagnosis *** Prompt diagnosis and treatment of CTX improve patient outcomes.

关键词： Cerebrotendinous xanthomatosis CYP27A1 Sterol 27-hydroxylase c.380G> c.1563dup A Case report

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Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer

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中国病理生理杂志 2012年第11期 2100-2101页

作者： ZHI Xiu-ling,yu Jian-gang,zhou Ping(Fudan University,Shanghai 200032)

AIM:CD73 is an ectoenzyme overexpressed in breast cancer cells,catalyzing the dephosphorylation of adenosine monophosphates into *** aim of this research is to explore the relation between CD73 and epidermal growth fa... 详细信息

关键词： CD EGFR

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THE STUDY OF PHOTOELECTROCHEHICAL CELLS BASED ON CHLOROPHYLL AND ORGANIC DYES

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Chinese Chemical Letters 1993年第8期4卷 721-724页

作者： Rui ling zhou yu Guo YANG yun yu HAN Department of Chemistry,Shandong Teachers’ University,Jinan 250014

Tetraiodofluorescein(TIF)and safranine T(ST)had great effects on the photovoltaic parameters of the *** Voc of the cells was about 3-5 times higher than that of the cells without TIK and ST,Isc increased 1 to 2 orders of *** Voc and Isc could be increased greatly only when Voc and Isc of the cell with Pt as WE properly combined with the Voc and Isc produced by chla in the original *** to absorption spectra and output characters,the results were elucidated.

关键词： TEOA THE STUDY OF PHOTOELECTROCHEHICAL CELLS BASED ON CHLOROPHYLL AND ORGANIC DYES ST

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Blockades of angiotensin and aldosterone reduce osteopontin expression and interstitial fibrosis infiltration in rats with myocardial infarction

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Chinese Medical Journal 2008年第21期121卷 2192-2196页

作者： ZHANG yu-ling zhou Shu-xian LEI Juan yuAN Gui-yi WANG Jing-feng Department of Cardiology Second Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510120 China

Background It has been reported that osteopontin has an important role in cardiac fibrosis and remodeling. However, its direct mechanisms remain unclear. The purpose of this study was to investigate the role of angiotensin and aldosterone blockades in cardiac osteopontin expression associated with cardiac remodeling in myocardial infarcted （MI） rats. Methods Fifty SD rats that survived 24 hours after ligating left anterior descending coronary artery were randomly divided into three groups： MI-saline group （n=15, 5 ml/d）, MI-perindopril group （n=18, perindopril 2 mg·kg^-1·d^-1） and MI-spironolacton （n=-17, spironolacton 20 mg·kg^-1·d^-1）. A sham operation group （n=15） was selected as non-infarcted control. At 6 weeks after treatment, hemodynamic pararmeters and left ventricular function were measured with catheterization, interstitial fibrosis infiltration and cardiomyocyte diameters were evaluated histologically. Myocardium osteopontin protein expression level in the non-infarcted myocardium was detected by Western blotting. Results No osteopontin protein was detected in the myocardium of sham-operation rats. High levels of osteopontin protein expression were detected in the MI-saline rats, but the levels were suppressed in the MI-perindopril and MI-spironolacton rats at 6 weeks following MI （P 〈0.01, respectively）. Compared with the sham operation group, all rats in the MI group showed marked interstitial fibrosis infiltration in the non-infarction area, higher ventricular weight/body weight ratio, significantly increased cardiomyocyte diameter （P 〈0.01, respectively）, and developed significant systolic and diastolic dysfunction as indicated by decreased left ventricular systolic pressure （LVSP） and ±dp/dt, as well as increased left ventricular end-diastolic pressure （LVEDP）（P 〈0.01, respectively）. Angiotensin and aldosterone blockades partly prevented cardiac fibrosis and systolic and diastolic dysfunction （P 〈0.01, respectively）. Conclusion Treatment with an

关键词： osteopontin myocardial infarction renin-angiotensin system

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Expression of tissue factor in rabbit pulmonary artery in an acute pulmonary embolism model

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World Journal of Emergency Medicine 2014年第2期5卷 144-147页

作者： Jing-xia Zhang Yong-li Chen yu-ling zhou Qian-yu Guo Xian-pei Wang Department of Cardiology Tianjin Chest Hospital

BACKGROUND: Tissue factor(TF) is the initiation factor of the extrinsic coagulation pathway, and plays a critical role in the process of thrombosis. This study aimed to investigate the expression of TF and to explore their clinical effect on the pulmonary artery after acute pulmonary ***: Thirty-four Japanese white rabbits(Level II animals) supplied by Tianjin Medical University were randomly assigned into: group A, specimens of the pulmonary artery taken 3 hours after pulmonary embolism(n=8); group B, specimens of the pulmonary artery taken 8 hours after pulmonary embolism(n=8); group C, specimens of the pulmonary artery taken 24 hours after pulmonary embolism(n=8); and control group, pseudo-operations performed without injection of autologous blood clots(n=10). The animal model of pulmonary thrombo-embolism was established by injection of autologous blood clots into the jugular vein through a 5F catheter, and was conf irmed by digital subtraction angiography. The mRNA expression of TF in different parts of the pulmonary artery was accessed by RT-PCR. The q test was used if there was a signif icant difference in a given continuous variable among the three groups assessed by ANOVA. The experiment equipment was supplied by the State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, the Chinese Academy of Medical Sciences and Peking Union Medical ***: The TF expression in the specimen adjacent to emboli was stable at 3, 8 or 24 hours after embolism. The mRNA expression of TF at 3 and 8 hours after embolism was lower in the specimens taken from the distal end of the morbid pulmonary artery than those adjacent to emboli. While at 24 hours after embolism, there were similar mRNA levels in specimens either adjacent or distal to ***: The high level of TF expression in pulmonary artery tissue adjacent to emboli could lead to locally increased coagulation activity, indicating the nece

关键词： Pulmonary embolism Tissue factor Pulmonary Gene expression Thrombus Anticoagulation Thrombolysis Rabbits

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Circ-Foxo3 positively regulates the Foxo3 gene and makes it a suppressor of acute myeloid leukemia

Circ-Foxo3 positively regulates the Foxo3 gene and makes it ...

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2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Jiao zhou Xi Tang Jing Zhang ling-yu zhou ling-ling Zhai Minse Evola-Deniz Vanessa yun yun Yi Jing Yi Jiang Lin Jun Qian Zhao-Qun Deng Department of Laboratory Center The Affiliated People's Hospital of Jiangsu University The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Department of Hematology The Affiliated People's Hospital of Jiangsu University

Objective:The Foxo3 gene, belonging to the forkhead family, is one of the classes of transcription factors characterized by a forkhead DNA-binding domain, which usually considered being a cancer suppressor ***-Foxo3 is a circular structurethat connects the 3’ endtothe5’end. Scholarsdetectedthat ectopicexpressed circ-Foxo3 could compete with Foxo3 for binding to some miRNAs.

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乌司他丁下调LPS诱导的小鼠巨噬细胞MiR-21表达及初步机制研究

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重庆医科大学学报 2017年第5期42卷 487-493页

作者：肖玲马渝周巧周发春重庆医科大学附属第一医院重症医学科重庆400016 重庆市急救医疗中心ICU 重庆400014

目的:观察在脂多糖(lipopolysaccharide,LPS)诱导的小鼠巨噬细胞株RAW264.7产生炎症反应中微小RNA-21(Micro RNA-21,mi R-21)的表达情况,以及乌司他丁(Ulinastatin,UTI)在炎症反应中对mi R-21的干预作用。方法:设置正常组、模型组(LPS 500 ng/m L剌激组)、实验组(LPS 500 ng/m L+UTI 1 000 U/m L组)和阴性实验对照组(UTI 1 000 U/m L),观察RAW264.7细胞生长状态;四甲基偶氮唑(methyl thiazolyl tetrazolium,MTT)法检测不同浓度UTI对RAW264.7细胞活性的影响;用不同浓度UTI预处理2 h后,LPS刺激小鼠RAW264.7细胞诱导炎症模型,RT-PCR检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)m RNA、白介素-6(interleukin-6,IL-6)m RNA、mi R-21的表达。结果:(1)UTI对RAW264.7细胞的毒性作用:UTI浓度小于1 000 U/m L时对RAW264.7细胞无毒性作用,比较差异无统计学意义(P=0.117)。(2)与正常组相比,在不同浓度(100,250,500 ng/m L)LPS刺激RAW264.7细胞后,TNF-αm RNA及IL-6 m RNA的分泌(1.311±0.037,1.549±0.039,1.667±0.059;1.167±0.019,1.518±0.058,1.740±0.071)与基础分泌量(1.000±0.000)相比明显升高(P=0.000),且mi R-21表达(1.082±0.114,1.671±0.087,2.789±0.107)明显升高,并随LPS浓度升高而增加(P=0.000);(3)不同浓度UTI(10,100,1 000 U/m L)能有效降低TNF-αm RNA及IL-6 m RNA(1.000±0.000,1.998±0.212,1.421±0.100,1.122±0.154,0.991±0.139;1.000±0.000,1.880±0.045,1.179±0.108,1.053±0.070,0.960±0.088)的表达,并降低mi R-21(1.000±0.000,2.789±0.107,2.675±0.135,2.258±0.170,1.369±0.279)的表达,差异有统计学意义(P1=0.000,P2=0.000,P3=0.000)。结论:LPS刺激小鼠RAW264.7细胞可促进mi R-21表达升高,其表达水平在一定程度上反映了细胞炎症反应状态;UTI可通过下调mi R-21抑制相关信号通路从而在免疫炎症反应中发挥保护作用。

关键词：微小RNA-21 乌司他丁炎性反应

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Low expression of FUS1 is negatively correlated with miR-378 and may predicts adverse prognosis in acute myeloid leukemia

Low expression of FUS1 is negatively correlated with miR-378...

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2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Xi Tang Jiao zhou Jing Zhang ling-yu zhou ling-ling Zhai Minse Evola-Deniz Vanessa yun yun Yi Jing Yi Jiang Lin Jun Qian Zhao-Qun Deng Department of Laboratory Center The Affiliated People's Hospital of Jiangsu University The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Department of Hematology The Affiliated People's Hospital of Jiangsu University

Introduction: FUS1 is a tumor suppressor gene that has been found frequently lost in a variety of solid tumors. In this study, we aimed to investigate the expression status of the FUS1 gene in acute myeloid leukemia(AML) and its clinical significances. At the same time to further explore the correlation between FUS1 and MiR-378 expression in AML. Methods: We detected the expression of FUS1 transcript in bone marrow mononuclear cells from 23 controls and 158 newly diagnosed AML patients by RQ-PCR. Results: Down-regulated FUS1 expression was found in 139/158(87.97%) AML cases, significantly lower than that in all 23 controls(P=0.012). ROC curve analysis revealed that the FUS1 transcript level could discriminate AML patients from controls effectively(AUC=0.663). Kaplan–Meier demonstrated that non-M3-AML patients with low FUS1 expression showed significantly shorter overall survival(OS, P=0.049) and leukemia free survival(LFS, P=0.051) than those with high FUS1 expression. However, Multivariate analysis showed that FUS1 overexpression was not an independent favorable prognostic factor for OS in non-M3 cohort of AML patients(HR = 0.731, 95% CI = 0.370–1.444, P=0.367). Furthermore, We studied the association of FUS1 and miR-378 expression in 53 newly diagnosed AML patients and found that the correlation coefficient was-0.346, showed FUS1 and miR-378 are negatively correlated in AML patients(P= 0.011). Conclusion: These results indicated that the low expression of FUS1 gene is a common molecular event in AML and its regulation of AML prognosis may be affected by miR-378.

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High expression of NANOG may predict a favorable outcome in acute myeloid leukemia

High expression of NANOG may predict a favorable outcome in ...

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2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

Objective:NANOG is an important transcription regulator that related to the self-renewal of embryonic stem cells (ESCs). Overexpression of NANOG has been proved to be associated with adverse prognosis in many solid tumors. In this present research, the point that we study on is how the NANOG gene affect the prognosis of AML patients.

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