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Elevational and temporal patterns of pollination success in distylous and homostylous buckwheats(Fagopyrum)in the Hengduan Mountains

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Plant Diversity 2024年第5期46卷 661-670页

作者： ling-yun Wu Shuang-Quan Huang Ze-Yu Tong Faculty of Resources and Environmental Science Hubei UniversityWuhan 430062China Institute of Evolution and Ecology School of Life SciencesCentral China Normal UniversityWuhan 430079China

Reproductive strategies of sexually dimorphic plants vary in response to the ***,we ask whether the sexual systems of Fagopyrum species(i.e.,selfing homostylous and out-crossing distylous)represent distinct adaptive strategies to increase reproductive success in changing alpine *** answer this question,we determined how spatial and temporal factors(e.g.,elevation and peak flowering time)affect reproductive success(i.e.,stigmatic pollen load)in nine wild Fagopyrum species(seven distylous and two homostylous)among 28 populations along an elevation gradient of 1299-3315 m in the Hengduan Mountains,southwestern *** also observed pollinators and conducted hundreds of hand pollinations to investigate inter/intra-morph compatibility,self-compatibility and pollen limitation in four Fagopyrum species(two distylous and two homostylous).We found that Fagopyrum species at higher elevation generally had bigger flowers and more stigmatic pollen loads;lateflowering individuals had smaller flowers and lower pollen *** pollen deposition was more variable in distylous species than in homostylous *** seed set was not pollenlimited in all species,we found that fruit set was much lower in distylous species,which rely on frequent pollinator visits,than in homostylous species capable of autonomous *** findings that pollination success increases at high elevations and decreases during the flowering season suggest that distylous and homostylous species have spatially and temporally distinct reproductive strategies related to environment-dependent pollinator activity.

关键词： Biodiversity hotspot Elevation gradient Fagopyrum Stigmatic pollen load Temporal pattern

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Effect of liquid surface depression size on discharge characteristics and chemical distribution in the plasma-liquid anode system

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Plasma Science and Technology 2024年第9期26卷 17-31页

作者： yun ling Dong DAI Jiaxin CHANG Buang WANG School of Electric Power South China University of TechnologyGuangzhou 510641People’s Republic of China University of Richmond Richmond 23173United States of America

Atmospheric pressure plasma-liquid interactions exist in a variety of applications,including wastewater treatment,wound sterilization,and *** practice,the phenomenon of liquid surface depression will inevitably *** applied gas will cause a depression on the liquid surface,which will undoubtedly affect the plasma generation and further affect the application ***,the effect of liquid surface deformation on the plasma is still *** this work,numerical models are developed to reveal the mechanism of liquid surface depressions affecting plasma discharge characteristics and the consequential distribution of plasma species,and further study the influence of liquid surface depressions of different sizes generated by different helium flow rates on the *** show that the liquid surface deformation changes the initial spatial electric field,resulting in the rearrangement of electrons on the liquid *** charges deposited on the liquid surface further increase the degree of distortion of the electric ***,the electric field and electron distribution affected by the liquid surface depression significantly influence the generation and distribution of active species,which determines the practical effectiveness of the relevant *** work explores the phenomenon of liquid surface depression,which has been neglected in previous related work,and contributes to further understanding of plasma-liquid interactions,providing better theoretical guidance for related applications and technologies.

关键词： plasma-liquid interaction deformation of the liquid surface liquid anode species fluxes

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Construction of CDKN2A-related competitive endogenous RNA network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1514-1531页

作者： Yong Pan Yi-Ru Zhang ling-yun Wang Li-Na Wu Ying-Qiu Ma Zhou Fang Shi-Bo Li Department of Infectious Disease Zhoushan HospitalWenzhou Medical UniversityZhoushan 316021Zhejiang ProvinceChina State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of Zhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Infectious Disease The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035Zhejiang ProvinceChina

BACKGROUND Competitive endogenous RNA(ceRNA)is an innovative way of gene expression modulation,which plays a crucial part in ***,the intricacy and behavioral characteristics of the ceRNA network in hepatocellular carcinoma(HCC)remain *** To establish a cyclin dependent kinase inhibitor 2A(CDKN2A)-related ceRNA network and recognize potential prognostic indicators for *** The mutation landscape of CDKN2A in HCC was first explored using the cBioPortal *** expression analysis was implemented between CDKN2Ahigh and CDKN2Alow expression HCC *** targeted microRNAs were predicted by lncBasev3.0,and the targeted mRNAs were predicted by miRDB,and Targetscan *** univariate and multivariate analysis were utilized to identify independent prognostic *** CDKN2A was frequently mutated and deleted in *** single-cell RNA-sequencing analysis revealed that CDKN2A participated in cell cycle *** CDKN2A-related ceRNA network-growth arrest specific 5(GAS5)/miR-25-3p/SRY-box transcription factor 11(SOX11)was successfully ***5 was recognized as an independent prognostic biomarker,whose overexpression was correlated with a poor prognosis in HCC *** association between GAS5 expression and methylation,immune infilt-ration was ***,traditional Chinese medicine effective components targeting GAS5 were *** This CDKN2A-related ceRNA network provides innovative insights into the molecular mechanism of HCC formation and ***,GAS5 might be a significant prognostic biomarker and therapeutic target in HCC.

关键词： Hepatocellular carcinoma Competitive endogenous RNA Molecular mechanism Prognosis Biomarker

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Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis

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Chinese Chemical Letters 2024年第8期35卷 413-417页

作者： Tian Feng yun-ling Gao Di Hu Ke-Yu Yuan Shu-Yi Gu Yao-Hua Gu Si-Yu Yu Jun Xiong Yu-Qi Feng Jie Wang Bi-Feng Yuan School of Public Health Research Center of Public HealthRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsWuhan 430071China University of Chinese Academy of Sciences Beijing 101408China Institute of Neuroscience and Brain Diseases Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441000China Phase I Clinical Trial Laboratory Zhongnan Hospital of Wuhan UniversityWuhan 430071China

Sleep deprivation(SD)is a widespread issue that disrupts the lives of millions of *** effects ini-tiate as changes within neurons,specifically at the DNA and RNA level,leading to disruptions in neuronal plasticity and the dysregulation of various cognitive functions,such as learning and *** acid epigenetic modifications that could regulate gene expression have been reported to play crucial roles in this ***,there is a lack of comprehensive research on the correlation of SD with nucleic acid epigenetic *** the current study,we aimed to systematically investigate the landscape of modifications in DNA as well as in small RNA molecules across multiple tissues,including the heart,liver,kidney,lung,hippocampus,and spleen,in response to chronic sleep deprivation(CSD).Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis,we characterized the dynamic changes in DNA and RNA modification profiles in different tissues of mice under CSD ***,we ob-served a significant decrease in the level of 5-methylcytosine(5mC)and a significant increase in the level of 5-hydroxymethylcytosine(5hmC)in the kidney in CSD *** RNA modifications,we observed an overall increased trend for most of these significantly changed modifications across six tis-sues in CSD *** study sheds light on the significance of DNA and RNA modifications as crucial epigenetic markers in the context of CSD-induced stress.

关键词： Sleep deprivation Epigenetics Mass spectrometry DNA modification RNA modification Small RNA

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含氮H13热作模具钢的热变形行为及热加工图

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材料热处理学报 2024年第1期45卷 185-192页

作者：原凌云山西太钢不锈钢股份有限公司技术中心山西太原030003

利用Gleeble-3800热模拟试验机,对含氮的H13热作模具钢开展了不同温度以及单道次的单向压缩试验,研究了其在应变速率为0.01~10 s^(-1)、变形温度为900~1250℃条件下的热变形行为,并根据高温应力-应变曲线,运用双曲正弦函数理论建立了该... 详细信息

利用Gleeble-3800热模拟试验机,对含氮的H13热作模具钢开展了不同温度以及单道次的单向压缩试验,研究了其在应变速率为0.01~10 s^(-1)、变形温度为900~1250℃条件下的热变形行为,并根据高温应力-应变曲线,运用双曲正弦函数理论建立了该钢的本构模型,并根据动态材料模型确定了其在不同应变下的热加工图。结果表明:高温热压缩变形状态下,含氮H13热作模具钢的流变应力随变形温度的升高而降低,随应变速率的降低而减小,当变形温度高于1100℃,应变速率小于0.1 s^(-1)时,含氮H13热作模具钢的流变应力曲线表现为动态再结晶特征;含氮H13热作模具钢的热变形激活能为610.23 kJ/mol,适合的热加工工艺范围为1100~1250℃,应变速率0.8~10 s^(-1)。

关键词：含氮H13钢热压缩变形本构方程热加工图

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2022年泸定M_(S)6.8地震震前区域变形背景及同震形变特征

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地震地质 2024年第3期46卷 645-664页

作者：徐晶季灵运刘传金中国地震局第二监测中心西安710054 地震动力学国家重点实验室中国地震局地质研究所北京100029

文中利用Sentinel-1 SAR影像获取了2022年泸定6.8级地震的震间形变场(2014—2020年)和同震形变场,估算了泸定地震的震间断层滑动速率、闭锁深度和同震滑动分布,分析了此次地震对周边断裂的影响,并进一步探讨泸定地震的发震构造和磨西断... 详细信息

文中利用Sentinel-1 SAR影像获取了2022年泸定6.8级地震的震间形变场(2014—2020年)和同震形变场,估算了泸定地震的震间断层滑动速率、闭锁深度和同震滑动分布,分析了此次地震对周边断裂的影响,并进一步探讨泸定地震的发震构造和磨西断裂及其周边断裂的未来地震发生趋势。震间InSAR形变场及InSAR和GNSS融合的三维形变场结果显示了2022年泸定地震发震断层的长期滑动特征,滑动速率为(5.9±1.8)mm/a,主要表现为剪切变形明显且应变集中的特征,震前磨西断裂存在明显闭锁,具有强震发生背景。同震滑动分布反演结果表明,此次泸定地震是以左旋走滑为主的高倾角走滑地震,最大滑动量可达1.71m,最大滑动位于10km深处。基于文中获得的同震滑动分布计算了同震位错效应引起的断层面上的库仑应力变化,结果显示磨西断裂南段的地震破裂段形成应力影区,而在此次地震中未破裂的北段的库仑应力显著升高,同时,折多塘断裂南东部分、安宁河断裂带石棉—冕宁段北西部分、大凉山断裂竹马段南东部分和公益海段南东部分的断层面库仑应力显著增加。2022年泸定地震的发震构造为鲜水河断裂带的磨西断裂,此次泸定地震的发生并没有完全降低鲜水河断裂带的地震风险,仍需重点关注磨西断裂未破裂段及其周边具有大地震发生背景的强震破裂空段。

关键词： 2022年泸定地震鲜水河断裂带震间形变同震滑动分布库仑应力变化地震危险性

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Function and Application Progress of Probiotics in Food

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Agricultural Biotechnology 2024年第4期13卷 63-64,69页

作者： Junpeng ZHAO Richard Avoi Azman Bin Atil@Azmi Jiao CHEN ling yun Tangshan Food and Drug Comprehensive Inspection and Testing Center Tangshan 063000China Hebei Agricultural Products Quality and Safety Testing Technology Innovation Center Tangshan 063000China Tangshan Institute of Industrial Technology for Functional Agricultural Products Tangshan 063000China Universiti Malaysia Sabah Faculty of Medicine and Health SciencesMalaysia Guizhou Forerunner College Guiyang 550000China Tangshan Center for Disease Prevention and Control Tangshan 063000China

Probiotics have a long history of use in human production and *** the gradual deepening of the research on the functions of probiotics,their various nutritional and health benefits for the human body have also become increasingly *** research shows that probiotics can regulate intestinal flora,and improve immunity and even the symptoms of some ***,in the food industry,the application of probiotics from traditional fermented foods to functional foods has become more and more *** this paper,the research progress of nutritional function and application of probiotics in food at home and abroad was reviewed,in order to provide some reference for the safe application of probiotics in food.

关键词： Probiotics Physiological function Food Development and application

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Determination of 14 Organophosphorus Pesticide Residues in Mutton by Gel Permeation Chromatography-Gas Chromatography-Mass Spectrometry(GPC-GC-MS)

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Agricultural Biotechnology 2024年第3期13卷 28-30,33页

[Objectives]This study was conducted to purify mutton samples by gel permeation chromatography(GPC).[Methods]Fourteen organophosphorus pesticide residues in samples were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS)in selective ion scanning mode(SIM).[Results]The organophosphorus pesticide standard solutions showed good linearity in the mass concentration range of 0.1-10.0μg/ml with correlation coefficients(r)not lower than 0.999,and the detection limits(S=3 N)ranged from 0.01 to 0.05 mg/*** average recovery values were in the range of 80.2%-99.7%,with relative standard deviations(RSDs,n=3)in the range of 1.8%-6.3%,at the addition levels of 0.5,1.0 and 2.0 mg/kg.[Conclusions]The method is simple,sensitive and accurate,and can be used for the determination of organophosphorus pesticide residues in mutton.

关键词： Mutton Gas chromatography-mass spectrometry Gel permeation chromatography Organophosphorus Pesticide residue

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Coca-Cola consumption vs fragmentation in the management of patients with phytobezoars:A prospective randomized controlled trial

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World Journal of Gastrointestinal Endoscopy 2024年第2期16卷 83-90页

作者： Fu-Guo Liu De-Feng Meng Xia Shen Dan Meng Ying Liu ling-yun Zhang Department of Gastroenterology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Endoscopy Center The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Gastric phytobezoars(GPBs)are very common in northern *** therapy involving carbonated beverage consumption and endoscopic lithotripsy has been shown to be effective and *** studies on this subject are often case reports highlighting the successful dissolution of phytobezoars through Coca-Cola ***,large-scale prospective investigations in this domain remain ***,we conducted a randomized controlled trial to examine the effects of Coca-Cola consumption on *** To evaluate the impact of Coca-Cola on GPBs,including the dissolution rate,medical expenses,ulcer rate,and operation *** A total of 160 consecutive patients diagnosed with GPBs were allocated into two groups(a control group and an intervention group)through computer-generated *** in the intervention group received a Coca-Cola-based regimen(Coca-Cola 2000-4000 mL per day for 7 d),while those in the control group underwent emergency *** Complete dissolution of GPBs was achieved in 100% of the patients in the intervention *** disparity in expenses between the control group and intervention group(t=25.791,P=0.000)was statistically significant,and the difference in gastric ulcer occurrence between the control group and intervention group(χ^(2)=6.181,P=0.013)was also statistically *** Timely ingestion of Coca-Cola yields significant benefits,including a complete dissolution rate of 100%,a low incidence of gastric ulcers,no need for fragmentation and reduced expenses.

关键词： Coca-Cola Bezoars Solubility Lithotripsy Gastrosco

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The potential role of Gegen Qinlian decoction in the treatment of coronavirus disease 2019 based on network pharmacology and validation of lipopolysaccharide-induced macrophages

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Integrative Medicine Discovery 2024年第24期8卷 1-8页

作者： Lei Wang ling-yun Wang Li Zhong Jian-Feng Shi Xiao-Ming Yao Wan-Wei Yang Department of Clinical Laboratory Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese medicineJiangsu Province Academy of Traditional Chinese MedicineNanjing 210028China

Background:Coronavirus disease 2019(COVID-19)is caused by severe acute respiratory syndrome coronavirus 2,which has led to deaths and currently lacks an efficient *** studies suggesting the potential of the Gegen Qinlian decoction(GQD)in preventing COVID-19,comprehensive analyses of its anti-COVID-19 potential are still ***:GQD treatment was evaluated for its efficacy in ameliorating the early stage(24 hours)of lipopolysaccharide(LPS)-induced cytokine storm in ***,target genes of GQD were co-analyzed with COVID-19 signature genes to identify key ingredients and their *** was also conducted using an LPS-induced macrophage ***:GQD treatment effectively ameliorated the early stage of LPS-induced cytokine storm in *** ingredients such as quercetin were found to be involved in multiple pathways,including inflammation,immunity,oxidative stress,cell proliferation,and apoptosis,through the AGE-RAGE signaling pathway and IL-17 signaling *** the LPS-induced macrophage model,quercetin inhibited macrophage polarization(M1)and the secretion of inflammatory factors(IL-6,TNF-α,IL-17A).Conclusions:Our results indicate that GQD can be utilized in the treatment of cytokine storm induced by COVID-19 and has the potential to treat COVID-19 by suppressing the COVID-19 signature genes and macrophage polarization.

关键词： Gegen Qinlian decoction coronavirus disease 2019 network pharmacology macrophage polarization

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