检索结果-南通市图书馆

MreBCD-associated Cytoskeleton is Required for Proper Segregation of the Chromosomal Terminus during the Division Cycle of Escherichia Coli

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2015年第9期128卷 1209-1214页

作者： Yu-Jia huo ling Qiao Xiao-Wei Zheng Cheng Cui Yuan-Fang Ma Feng Lu Henan Engineering Laboratory of Antibody Medicine Medical School of Henan University Kaifeng Henan 475001 China

Background：In prokaryotic organisms,the mechanism responsible for the accurate partition of newly replicated chromosomes into daughter cells is incompletely *** of the replication terminus of the circular prokaryotic chromosome poses special problems that have not previously been *** aim of this study was to investigate the roles of several protein components （MreB,MreC,and MreD） of the prokaryotic cytoskeleton for the faithful transmission of the chromosomal terminus into daughter ***：Strain LQ1 （mreB：：cat）,LQ2 （mreC：：cat）,and LQ3 （mreD：：cat） were constructed using the Red recombination ***11/pLAU53,LQ12/pLAU53,LQ13/pLAU53,LQ14/pLAU53,and LQ15/pLAU53 strains were generated by Pltransduction of （tetO）240-Gm and （lacO）240-Km cassettes from strains IL2 and *** microscopy was performed to observe localization pattern of fluorescently-labeled origin and terminus foci in wild-type and mutant *** induction was monitored as gfp fluorescence from PsulA-gfp in log phase cells grown in Luria-Bertani medium at 37℃ by measurement of emission at 525 nm with excitation at 470 nm in a microplate fluorescence ***：Mutational deletion of the mreB,mreC,or mreD genes was associated with selective loss of the terminus region in approximately 40％ of the cells within growing *** was accompanied by significant induction of the SOS DNA damage response,suggesting that deletion of terminus sequences may have occurred by chromosomal cleavage,presumably caused by ingrowth of the division septum prior to segregation of the replicated ***：These results imply a role for the MreBCD cytoskeleton in the resolution of the final products of terminus replication and/ or in the specific movement of newly replicated termini away from midcell prior to completion of septal *** would identify a previously unrecognized stage in the overall process of chromosome segregation.

关键词： Cytoskeleton Escherichia Coli MreBCD Segregation of Chromosomal Terminus

Early prediction of pathological outcomes to neoadjuvant chemotherapy in breast cancer patients using automated breast ultrasound

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2016年第5期28卷 478-485页

作者： Xinguang Wang ling huo Yingjian He Zhaoqing Fan Tianfeng Wang Yuntao Xie Jinfeng Li Tao Ouyang Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Breast Center Peking University Cancer Hospital & Institute Beijing 100142 China

Objective： Early assessment of response to neoadjuvant chemotherapy （NAC） for breast cancer allows therapy to be individualized. The optimal assessment method has not been established. We investigated the accuracy of automated breast ultrasound （ABUS） to predict pathological outcomes after NAC. Methods： A total of 290 breast cancer patients were eligible for this study. Tumor response after 2 cycles of chemotherapy was assessed using the product change of two largest perpendicular diameters （PC） or the longest diameter change （LDC）. PC and LDC were analyzed on the axial and the coronal planes respectively. Receiver operating characteristic （ROC） curves were used to evaluate overall performance of the prediction methods. Youden＇s indexes were calculated to select the optimal cut-off value for each method. Sensitivity, specificity, positive and negative predictive values （PPV and NPV） and the area under the ROC curve （AUC） were calculated ***： ypT0/is was achieved in 42 patients （14.5%） while ypT0 was achieved in 30 patients （10.3%） after NAC. All four prediction methods （PC on axial planes, LDC on axial planes, PC on coronal planes and LDC on coronal planes） displayed high AUCs （all〉0.82）, with the highest of 0.89 [95% confidence interval （95% CI）, 0.83-0.95] when mid-treatment ＆BUS was used to predict final pathological complete remission （pCR）. High sensitivities （85.7%-88.1%） were observed across all four prediction methods while high specificities （81.5%-85.1%） were observed in two methods used PC. The optimal cut-off values defined by our data replicate the WHO and the RECIST criteria. Lower AUCs were observed when mid-treatment ABUS was used to predict poor pathological outcomes. Conclusions：ABUS is a useful tool in early evaluation of pCR after NAC while less reliable when predicting poor pathological outcomes.

关键词： Automated breast ultrasound breast neoplasms drug monitoring neoadjuvant therapy pathologicalcomplete remission ultrasonography

在线全文

学校读者我要写书评

暂无评论

红枣规模化生产地区机械化现状及发展对策

中国农机装备 2024年第5期 110-112页

作者：马木提江草鲁孟霍玲阿孜古力孙涛新疆巴音郭楞蒙古自治州农业农村机械化发展中心库尔勒841000 不详

红枣作为重要的农作物之一,对于农业经济的发展具有重要意义。红枣的机械化生产作业在节省人工劳作量的情况下,可以显著提升作业效率,提升红枣的产量与品质。但是,随着红枣种植业规模的逐渐扩大,出现了管理力度不足导致机械化效率降低... 详细信息

红枣作为重要的农作物之一,对于农业经济的发展具有重要意义。红枣的机械化生产作业在节省人工劳作量的情况下,可以显著提升作业效率,提升红枣的产量与品质。但是,随着红枣种植业规模的逐渐扩大,出现了管理力度不足导致机械化效率降低的问题。本文在阐述红枣规模化生产地区机械化现状的基础上,提出了发展红枣生产机械化的对策,以促进红枣种植业的可持续发展。

关键词：红枣机械化生产现状对策

Transgenic Rape with hrf2 Gene Encoding Harpin_(Xooc) Resistant to Sclerotinia sclerotinorium

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第4期7卷 455-461页

作者： MA ling-li huo Rong GAO Xue-wen HE Dan SHAO Min WANG Qi Key Laboratory of Monitoring and Management of Plant Diseases and Pests Ministry of Agriculture/Department of Plant PathologyNanjing Agricultural University Nanjing 210095 P.R.China

The objective of this study was to increase the resistance of rape using the method of transformation. The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinxooc protein was constructed into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. Hygromycin-resistant green shoots were obtained. Successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4 was confirmed by PCR, RT-PCR, and β-glucuronidase analyses. Disease bioassays of transgenic plants revealed an improved resistance of transgenic plants to Rape sclerotiniose. In brief, the hrf2 gene can be transferred into rape using the method of Agrobacterium-mediated transformation, which increased the resistance to Sclerotinia sclerotinorium in the transgenic plant.

关键词： hrf2 gene Agrobacterium tumefaciens-mediated transformation transgenic oilseed rape resistance

Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2024年第2期26卷 165-174页

作者： Hai-Wei Feng Yu Zhao Yan-ling Gao Dong-Teng Liu Li-Jun huo Key Laboratory of Agricultural Animal Genetics Breeding and ReproductionEducation Ministry of ChinaCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Shenzhen Key Laboratory of Fertility Regulation Reproductive Medicine CenterThe University of Hong Kong-Shenzhen HospitalShenzhen 518053China Maternal-Fetal Medicine Institute Department of Obstetrics and GynaecologyShenzhen Baoan Women's and Children's HospitalJinan UniversityShenzhen 518100China Department of Obstetrics and Gynaecology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina

Meiosis is the process of producing haploid gametes through a series of complex chromosomal events and the coordinated action of various *** mitochondrial protease complex(ClpXP),which consists of caseinolytic mitochondrial matrix peptidase X(ClpX)and caseinolytic protease P(ClpP)and mediates the degradation of misfolded,damaged,and oxidized proteins,is essential for maintaining mitochondrial *** has been implicated in meiosis regulation,but its precise role is currently *** this study,we engineered an inducible male germ cell-specific knockout caseinolytic mitochondrial matrix peptidase X(Clpx^(cKO))mouse model to investigate the function of ClpX in *** found that disrupting Clpx in male mice induced germ cell apoptosis and led to an absence of sperm in the ***,it caused asynapsis of homologous chromosomes and impaired meiotic recombination,resulting in meiotic arrest in the zygotene-to-pachytene transition *** loss of ClpX compromised the double-strand break(DSB)repair machinery by markedly reducing the recruitment of DNA repair protein RAD51 homolog 1(RAD51)to DSB *** dysfunction may be due to an insufficient supply of energy from the aberrant mitochondria in Clpx^(cKO) spermatocytes,as discerned by electron ***,ubiquitination signals on chromosomes and the expression of oxidative phosphorylation subunits were both significantly attenuated in Clpx^(cKO) *** together,we propose that ClpX is essential for maintaining mitochondrial protein homeostasis and ensuring homologous chromosome pairing,synapsis,and recombination in spermatocytes during meiotic prophase I.

关键词： ClpX homologous chromosome meiosis mitochondrial recombination synapsis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

设施蔬菜育苗机械化精准播种技术

中国农机装备 2024年第6期 68-70页

作者：孙涛赵黎炜买买提江·艾克木霍玲阿孜古力马木提江新疆巴音郭楞蒙古自治州农业农村机械化发展中心库尔勒841000 不详

采用机械化蔬菜播种技术以及育苗技术能够提升蔬菜后续的生长能力,提升产量以及品质。在阐述设施蔬菜机械化育苗技术的基础上,对设施蔬菜机械化育苗的前景以及设施蔬菜播种技术进行分析,旨在为进一步提升设施蔬菜育苗机械化水平以及精... 详细信息

采用机械化蔬菜播种技术以及育苗技术能够提升蔬菜后续的生长能力,提升产量以及品质。在阐述设施蔬菜机械化育苗技术的基础上,对设施蔬菜机械化育苗的前景以及设施蔬菜播种技术进行分析,旨在为进一步提升设施蔬菜育苗机械化水平以及精准播种技术水平提供参考。

关键词：蔬菜育苗机械化科学播种技术分析

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

推动腐屑资源利用促进循环经济发展

生态经济 2005年第9期21卷 72-74页

作者：霍玲石家庄经济学院经济研究所石家庄050031

循环经济思想是当前促进国民经济与生态环境健康协调发展的重要指导思想。推动腐屑资源利用是循环经济思想在农业领域的重要实现途径。腐屑资源利用符合大农业体系的基本框架,而大农业体系又是全部农业范畴与循环经济思想的有机结合。... 详细信息

循环经济思想是当前促进国民经济与生态环境健康协调发展的重要指导思想。推动腐屑资源利用是循环经济思想在农业领域的重要实现途径。腐屑资源利用符合大农业体系的基本框架,而大农业体系又是全部农业范畴与循环经济思想的有机结合。我们应构建腐屑生态体系,促进循环经济发展,迎接新的历史性的农业变革。

关键词：腐屑资源农业对策循环经济大农业资源利用经济发展循环经济思想农业领域农业体系

在线全文

学校读者我要写书评

暂无评论

专业伦理与工作的意义

华中科技大学学报（社会科学版） 2011年第4期25卷 1-9,14页

作者：林火旺台湾大学哲学系

社会是一种分工,每一个行业存在的目的都是为了实现一种价值,譬如:医生的目的是为了健康;建筑师的目的是为了舒适、安全的房舍和桥梁;司法人员的目的是为了维护社会的公平正义。而要求每一个行业必须遵守和该行业相关的伦理道德,目的则... 详细信息

社会是一种分工,每一个行业存在的目的都是为了实现一种价值,譬如:医生的目的是为了健康;建筑师的目的是为了舒适、安全的房舍和桥梁;司法人员的目的是为了维护社会的公平正义。而要求每一个行业必须遵守和该行业相关的伦理道德,目的则是为了确保行业或职业价值的实现,唯此才能使社会所有成员因分工而享受彼此的成果。只重视职业的外在价值,而忽视职业伦理的人,不只无法达成社会分工的任务,也无法实现工作本身的内在价值。而违反职业道德不只对社会上其他人不利,也会危及个人的幸福。

关键词：专业伦理社会正义幸福

Core and variable antimicrobial resistance genes in the gut microbiomes of Chinese and European pigs

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2024年第1期45卷 189-200页

作者： Cui-Hong Tong Zhi-Peng huo Lu Diao Dan-Yu Xiao Ruo-Nan Zhao Zhen-ling Zeng Wen-Guang Xiong Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation South China Agricultural UniversityGuangzhouGuangdong 510642China National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria College of Veterinary MedicineSouth China Agricultural UniversityGuangzhouGuangdong 510642China National Laboratory of Safety Evaluation(Environmental Assessment)of Veterinary Drugs South China Agricultural UniversityGuangzhouGuangdong 510642China National Reference Laboratory of Veterinary Drug Residues South China Agricultural UniversityGuangzhouGuangdong 510642China

Monitoring the prevalence of antimicrobial resistance genes(ARGs)is vital for addressing the global crisis of antibiotic-resistant bacterial *** its importance,the characterization of ARGs and microbiome structures,as well as the identification of indicators for routine ARG monitoring in pig farms,are still lacking,particularly concerning variations in antimicrobial exposure in different countries or ***,metagenomics and random forest machine learning were used to elucidate the ARG profiles,microbiome structures,and ARG contamination indicators in pig manure under different antimicrobial pressures between China and *** showed that Chinese pigs exposed to high-level antimicrobials exhibited higher total and plasmid-mediated ARG abundances compared to those in European pigs(P<0.05).ANT(6)-Ib,APH(3')-IIIa,and tet(40)were identified as shared core ARGs between the two pig ***,the core ARGs identified in pig populations were correlated with those found in human populations within the same geographical *** and Prevotella were identified as the dominant genera in the core microbiomes of Chinese and European pigs,*** ARG markers and 43 biomarkers were able to differentiate between the Chinese and European pig manure samples with accuracies of 100%and 98.7%,*** for assessing ARG contamination in Chinese and European pigs also achieved high accuracy(r=0.72-0.88).Escherichia flexneri in both Chinese and European pig populations carried between 21 and 37 *** results of this study emphasize the importance of global collaboration in reducing antimicrobial resistance risk and provide validated indicators for evaluating the risk of ARG contamination in pig farms.

关键词： Metagenomic Pig manure Antimicrobial pressure Antimicrobial resistance genes Microbiome