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Effects of Transcutaneous Electrical Acupoint Stimulation on Ovarian Responses and Pregnancy Outcomes in Patients Undergoing IVF-ET:A Randomized Controlled Trial

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Chinese Journal of Integrative Medicine 2022年第5期28卷 434-439页

作者： ZHAI Zhi-jin LIU Jia-en lei ling-ling WANG Shu-yu Department of Reproductive Medicine Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing Maternal and Child Health Care HospitalBeijing(100026)China Center for Reproductive Medicine Beijing Jia-En De Yun HospitalBeijing(100089)China

Objective:To evaluate the influence of different transcutaneous electrical acupoint stimulation(TEAS)modes on ovarian responses and pregnancy outcomes in patients with infertility undergoing in vitro fertilization and embryo transfer(IVF-ET).Methods:Two hundred infertility patients undergoing IVF-ET were divided randomly into experimental groups(TEAS groups:E-Ⅰ,E-Ⅱ,E-Ⅲ,and E-Ⅳ,40 cases each group)and a control group(mock TEAS group,40 patients)using the random number *** patients in the experimental groups received TEAS treatment of 20,30,40 and 50 m A for the E-Ⅰ,E-Ⅱ,E-Ⅲand E-Ⅳgroups,*** control group received a treatment of 5 m *** was applied at acupoints of Guanyuan(RN 4),Zhongji(RN 3),Sanyinjiao(SP 6),Zigong(EX-CA 1),and Taixi(KI 13),once a day for 30 min each time for a treatment period of 10-13 *** effect was assessed using the following indicators:endometrial thickness on the 6 th day of gonadotropin treatment(GN6 day),endometrial thickness on the day on chorionic gonadotropin administration(HCG day),number of ovarian follicles on HCG day,number of ova captured,amount of estrogen required for each harvested ova,number of mature ova divided by the total number of ova,percentage of high-quality embryos,and clinical ***:Endometrial thickness in the experimental groups on the HCG day was significantly better than that of the control group after TEAS stimulation(P=0.01).TEAS exhibited a greater impact on the number of ova captured(P=0.003).However,the effect of TEAS stimulation on the high-quality embryo rate and clinical pregnancy in patients was not statistically significant(P>0.05).Conclusions:TEAS is an effective method in improving the ovarian *** the stimulus intensity was at 40 m A and above,it could be helpful to improve the patient’s endometrial condition and endometrial receptivity and to retrieve more oocytes.(Trial registration *** CTR-TRC-11001780)

关键词： acupuncture transcutaneous electrical acupoint stimulation in vitro fertilization and embryo transfer pregnancy outcome infertility controlled ovarian hyperstimulation

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Recent advances in hydrothermal modification of calcium phosphorus coating on magnesium alloy

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Journal of Magnesium and Alloys 2022年第1期10卷 66-87页

作者： lei ling Shu Cai Qianqian Li Jiayue Sun Xiaogang Bao Guohua Xu Key Laboratory for Advanced Ceramics and Machining Technology of Ministry of Education Tianjin UniversityTianjinChina Department of Orthopedic Surgery Spine CenterChangzheng HospitalNaval Medical UniversityShanghaiChina

Magnesium(Mg) and its alloys have emerged as a favored candidate for bio-regenerative medical implants due to their superior biocompatibility, biodegradability and the elastic modulus close to that of human bone. Unfortunately, the rapid and uncontrollable degradation rate of Mg alloys in chloride-rich body microenvironments limits their clinical orthopedic applications. Recently, Calcium Phosphate(Ca-P)biomaterials, especially Hydroxyapatite(HA), have been broadly applied in the surface functional modification of metal-based biomaterials attributed to their excellent bioactivity and biocompatibility. Hydrothermal modification of Ca-P coatings on Mg alloys has been extensively exploited by researchers for its significant superiorities in controlling coating structure and improving interfacial bonding strength for better osseointegration and corrosion resistance. This work focuses on the up-to-the-minute advances in Ca-P coatings on the surface of Mg and its alloys via hydrothermal methods, including the strategies and mechanisms of hydrothermal modification. Herein, we are inclined to share some feasible and attractive hydrothermal surface modification strategies. From the perspectives of hydrothermal manufacturing technique innovation and coating structure optimization, we evaluate how to foster the corrosion resistance, coating bonding strength, osseointegration and antibacterial properties of Mg alloys with Ca-P coatings synthesized by hydrothermal method. The challenges and future perspectives on the follow-up exploration of Mg alloys for orthopedic applications are also elaborately proposed.

关键词： Calcium phosphorus Magnesium alloy Coatings Hydrothermal methods Corrosion resistance Biological properties

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Process conditions for preparing methanol from cornstalk gas

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Journal of Environmental Sciences 2007年第5期19卷 628-632页

作者： ZHU ling-feng DU lei LI Xin-bao LI Guo-ting ZHANG Jie Department of Environmental Engineering North China Institute of Water Conservancy and Hydroelectric Power Zhengzhou 450011 China

The low-heat-value cornstalk gas produced in the down-flow fixed bed gasifier was tentatively used for methanol synthesis. The cornstalk gas was purified and the technical procedures such as deoxygenation, desulfurization, catalytic cracking of tar, purification and hydrogenation were studied. The catalytic experiments of methanol synthesis with cornstalk syngas were carried out in a tubular-flow integral and isothermal reactor. The effect of reaction temperature, pressure, catalysttypes, catalyst particle size, syngas flow at entering end and composition of syngas was investigated. The optimum process conditions and yield of methanol from cornstalk syngas were obtained. The experimental results indicated that the proper catalyst of the synthetic reaction was C301 and the optimum catalyst size （φ） was 0.833 mm×0.351 mm. The optimum operating temperature and pressure were found to be 235℃ and 5 Mpa, respectively. The suitable syngas flow 0.9-1.10 mol/h at entering end was selected and the best composition of syngas were CO 10.49%, CO2 8.8%, N2 37.32%, CnHm 0.95% and H2 40.49%. The best methanol yield is 0.418 g/g cornstalk. The study provided the technical support for the industrial test of methanol production from biomass （cornstalk）gas.

关键词： cornstalk syngas fuel gas purification catalyst methanol synthesis

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First-principles insight into Li and Na ion storage in graphene oxide

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Chinese Physics B 2019年第7期28卷 517-522页

作者： Shu-Ying Zhong Jing Shi Wen-Wei Luo Xue-ling lei Institute of Physics and Communication & Electronics Jiangxi Normal University

The structural, electronic, and adsorption properties of Li/Na ions on graphene decorated by epoxy groups are investigated by first-principles calculations based on density functional *** results show that the concentration of epoxy groups remarkably affects the structural and electronic properties of *** bandgaps change monotonically from0.16 eV to 3.35 eV when the O coverage increases from 12.5% to 50%(O/C ratio).Furthermore, the highest lithiation potential of 2.714 V is obtained for the case of graphene oxide(GO) with 37.5 % O coverage, while the highest sodiation potential is 1.503 V for GO with 12.5% O *** clearly demonstrates that the concentration of epoxy groups has different effects on Li and Na storage in *** results provide a new insight into enhancing the Li and Na storage by tuning the concentration of epoxy groups on GO.

关键词： graphene oxide lithiation potential sodiation potential first-principles

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AN INVESTIGATION OF RETINEX ALGORITHMS FOR IMAGE ENHANCEMENT

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Journal of Electronics(China) 2007年第5期24卷 696-700页

作者： lei ling Zhou Yinqing Li Jingwen 201 Lab Beijing University of Aeronautics and Astronautics Beijing 100083 China

This letter introduces color constancy and Retinex theory for image *** clas- sifies Retinex algorithms into four categories and provides their principles and implementations in *** experimental results of Frankle-McCann,MSR (Multi-Scale Retinex) and PNSD (Pro- jected Normalized Steepest Descent) Retinex algorithms are presented and ***, variance and average gradient are proposed to evaluate the performance of the different algorithms.

关键词： Color constancy Retinex Frankle-McCann MSR (Multi-Scale Retinex) PNSD (Pro-jected Normalized Steepest Descent)

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Clinical outcomes with large macular holes using the tiled transplantation internal limiting membrane pedicle flap technique

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International Journal of Ophthalmology(English edition) 2019年第2期12卷 246-251页

作者： Li-Ping Wang Wen-Tao Sun Chun-ling lei Jin Deng Department of Ophthalmology Shaanxi Ophthalmic Medical Center Xi'an Fourth Hospital Affiliated Guangren HospitalSchool of Medicine Xi'an Jiaotong University

AIM: To report the surgical technique and efficacy of the tiled transplantation internal limiting membrane(ILM) pedicle flap technique after vitrectomy for 33 patients with large macular hole(MH). METHODS: This study was a prospective noncontrolled interventional study. All patients were treated by vitrectomy, the tiled transplantation ILM pedicle flap and gas tamponade. All patients underwent visual acuity measurements and optical coherence tomography(OCT), during preoperative and the follow-up visits postoperative. RESULTS: Two high-myopic patient had flap dislocation during surgery. The thorough closure of MH following the tiled transplantation ILM pedicle flap technique was ultimately achieved in 31 patients observed by OCT imaging(93.94%) 1 wk after surgery. Visual acuity improved from 1.51±0.31(logMAR) preoperative to 0.92±0.30 6 mo after surgery(P=0.000). There were no significant changes in OCT finding during the follow-up period from 1 mo to 6 mo after surgery. CONCLUSION: The tiled transplantation ILM pedicle flap technique provides bridge for retinal gliosis to achieve successful closures of the large MHs, and the microenvironment of this technique is more similar to the normal physiological conditions.

关键词： tiled transplantation ILM pedicle flap macular hole vitrectomy

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An Improved Soft Subspace Clustering Algorithm for Brain MR Image Segmentation

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Computer Modeling in Engineering & Sciences 2023年第12期137卷 2353-2379页

作者： lei ling Lijun Huang Jie Wang Li Zhang Yue Wu Yizhang Jiang Kaijian Xia School of Artificial Intelligence and Computer Science Jiangnan UniversityWuxi214122China Department of Scientific Research Changshu Hospital Affiliated to Soochow UniversityChangshu215500China China Department of Biomedical Engineering Faculty of EngineeringUniversiti MalayaKuala Lumpur50603Malaysia

In recent years,the soft subspace clustering algorithm has shown good results for high-dimensional data,which can assign different weights to each cluster class and use weights to measure the contribution of each dimension in various *** enhanced soft subspace clustering algorithm combines interclass separation and intraclass tightness information,which has strong results for image segmentation,but the clustering algorithm is vulnerable to noisy data and dependence on the initialized clustering ***,the clustering algorithmis susceptible to the influence of noisydata and reliance on initializedclustering centers andfalls into a local optimum;the clustering effect is poor for brain MR images with unclear boundaries and noise *** address these problems,a soft subspace clustering algorithm for brain MR images based on genetic algorithm optimization is proposed,which combines the generalized noise technique,relaxes the equational weight constraint in the objective function as the boundary constraint,and uses a genetic algorithm as a method to optimize the initialized clustering *** genetic algorithm finds the best clustering center and reduces the algorithm’s dependence on the initial clustering *** experiment verifies the robustness of the algorithm,as well as the noise immunity in various ways and shows good results on the common dataset and the brain MR images provided by the Changshu First People’s Hospital with specific high accuracy for clinical medicine.

关键词： Soft subspace clustering image segmentation genetic algorithm generalized noise brain MR images

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Interactive Web-based Annotation of Plant MicroRNAs with iwa-miRNA

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Genomics, Proteomics & Bioinformatics 2022年第3期20卷 557-567页

作者： Ting Zhang Jingjing Zhai Xiaorong Zhang lei ling Menghan Li Shang Xie Minggui Song Chuang Ma State Key Laboratory of Crop Stress Biology for Arid Areas Center of BioinformaticsCollege of Life SciencesNorthwest A&F UniversityYangling 712100China Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region Ministry of Agriculture and Rural AffairsNorthwest A&F UniversityYangling 712100China College of Plant Science Tibet Agricultural and Animal Husbandry UniversityLinzhi 860006China College of Information Engineering Northwest A&F UniversityYangling 712100China

MicroRNAs(miRNAs) are important regulators of gene expression. The large-scale detection and profiling of miRNAs have been accelerated with the development of highthroughput small RNA sequencing(sRNA-Seq) techniques and bioinformatics tools. However,generating high-quality comprehensive miRNA annotations remains challenging due to the intrinsic complexity of sRNA-Seq data and inherent limitations of existing miRNA prediction tools. Here,we present iwa-miRNA, a Galaxy-based framework that can facilitate miRNA annotation in plant species by combining computational analysis and manual curation. iwa-miRNA is specifically designed to generate a comprehensive list of miRNA candidates, bridging the gap between already annotated miRNAs provided by public miRNA databases and new predictions from sRNA-Seq datasets. It can also assist users in selecting promising miRNA candidates in an interactive mode,contributing to the accessibility and reproducibility of genome-wide miRNA annotation. iwamiRNA is user-friendly and can be easily deployed as a web application for researchers without programming experience. With fexible, interactive, and easy-to-use features, iwa-miRNA is a valuable tool for the annotation of miRNAs in plant species with reference genomes. We also illustrate the application of iwa-miRNA for miRNA annotation using data from plant species with varying genomic complexity. The source codes and web server of iwa-miRNA are freely accessible at http://***/.

关键词： Galaxy Interactive annotation Manual inspection MicroRNA Platform

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Effect of screening colonoscopy frequency on colorectal cancer mortality in patients with a family history of colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 354-363页

作者： Li Zheng Bin Li ling lei Li-Jia Wang Zhi-Ping Zeng Jian-Dong Yang Department of Gastroenterology The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina Department of General Surgery The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina

BACKGROUND Colorectal cancer is a common malignant tumor in China,and its incidence in the elderly is increasing *** bowel disease is a group of chronic non-specific intestinal inflammatory diseases,including ulcerative colitis and Crohn’s *** included the clinicopathological and follow-up data of patients with colorectal cancer who underwent laparoscopic colectomy or open colectomy at our Gastrointestinal Department between January 2019 and December *** indicators,oncological indicators,and survival rates were compared between the *** results of 104 patients who met the above criteria were extracted from the database(laparoscopic colectomy group=63,open colectomy group=41),and there were no statistically significant differences in the baseline data or follow-up time between the two *** Intraoperative blood loss,time to first ambulation,and time to first fluid intake were significantly lower in the laparoscopic colectomy group than in the open colectomy *** differences in overall mortality,tumor-related mortality,and recurrence rates between the two groups were not statistically significant,and survival analysis showed that the differences in the cumulative overall survival,tumor-related survival,and cumulative recurrence-free rates between the two groups were not statistically *** In elderly patients with colorectal cancer,laparoscopic colectomy has better short-term outcomes than open colectomy,and laparoscopic colectomy has superior long-term survival outcomes compared with open colectomy.

关键词： Colorectal cancer Laparoscopic surgery Open surgery Prognosis Laparoscopic colectomy Open colectomy

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Clinical characteristics,molecular epidemiology and antimicrobial susceptibility of pertussis among children in southern China

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World Journal of Pediatrics 2020年第2期16卷 185-192页

作者： Jiao-Sheng Zhang Hong-Mei Wang Kai-Hu Yao Ying Liu Yan-ling lei Ji-Kui Deng Yong-Hong Yang Laboratory of Microbiology and Immunology Beijing Children's Hospital Affiliated to Capital Medical University56 Nanlishi RoadBeijing 100045China Department of Infectious Diseases Shenzhen Children's HospitalShenzhen 518038China

Background Increasing numbers of pertussis cases have been reported in recent *** reported cases from Shenzhen Children's Hospital were close to one tenth of all cases in *** epidemiology of antigenic genotype and antibiotic resistance of circulating strains in children have been unknown in Shenzhen,southern *** aim of this study was to describe the clinical features and explore the genotypes and antimicrobial susceptibility of circulating Bordetella pertussis among children in *** Data of hospitalized children with pertussis in Shenzhen Children's Hospital from August 2015 to April 2017 were *** genetic variability of isolates was investigated and Etest was performed for phenotypic susceptibility to erythromycin,azithromycin,clarithromycin,clindamycin,and trimethoprim/*** 469 children with pertussis confirmed by real-time quantitative polymerase chain reaction were hospitalized and strains were isolated from 105 *** blood cell count≥20×10^9/L and lymphocyte proportion≥60%were observed in 39.29%of infants younger than 3 *** two predominant profiles of virulence-associated allelic genes were ptxA1/ptxC1/ptxPl/prnl(48.6%)and ptxA1/ptxC2/ptxP3/prn2(44.8%).Among the isolates,48.6%(51/105)were found resistant to *** These findings indicate that leukocytosis is not a sensitive indicator of *** with the gene profile ptxP3/prn2 were highly circulating in Shenzhen and less resistant to macrolides,different from patterns observed in other parts of China.

关键词： Antimicrobial susceptibility Bordetella pertussis Children Genotype Virulence

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