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Cosuppression of RBCS3B in Arabidopsis leads to severe photoinhibition caused by ROS accumulation

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Cosuppression of RBCS3B in Arabidopsis leads to severe photo...

第十四届全国植物基因组学大会

作者： Gaomiao Zhan Zhiyong Hu Jing Liu Rongjun Li linbin deng Hanzhong Wang Wei Hua Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

As the most abundant protein on Earth,Rubisco has received much attention in the past *** so,its function is still not understood *** this paper, four Arabidopsis transgenic lines(RBCS3B-7,18,33,and 35) with albino or... 详细信息

关键词： RBCS3B cosuppression photoinhibition ROS Arabidopsis

Large-Scale Identification of Genome-Wide Single-Nucleotide Polymorphisms in polyploidy crop oilseed rape

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Large-Scale Identification of Genome-Wide Single-Nucleotide ...

第十三届全国植物基因组学大会

作者： Shunmou Huang,linbin deng,Shengyi Liu,Wei Hua~* Oil Crops Research Institute of The Chinese Academy of Agricultural Sciences,Wuhan 430062,China

Single nucleotide polymorphisms(SNPs) are the most abundant form of genetic variation existing between any diverse genotypes.A large number of available SNPs would be helpful for map-based cloning,genome-wide associat...

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Effective method for isolating liquid endosperm and improved procedure for subsequent DNA extraction in Brassica napus

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Oil Crop Science 2016年第2期1卷 20-25页

作者： Jun LI Shihang FAN Xingchao SUN Zhiyong HU Jing LIU Xiaoyi ZHU Ming ZHENG Fengming SUN Hongli YANG Liang ZHANG linbin deng Hongfang LIU Wei HUA Key Laboratory of Biology and Genetic Improvemtent of Oil Crops Ministry of AgricultureOil Crops Research InstituteChinese Academy of Agriculture Sciences

The important agronomic traits of seed size and seed oil content in oilseed rape were af-fected by maternal effect. The maternal tissue endosperm, generated by fertilization of the diploid central cell in the female gametophyte, is essential for embryo and seed development. Imprinting is primarily re-stricted to the endosperm of flowering plants and is associated with differential DNA methylation of paren-tal alleles. Therefore, it is necessary to find out whether the endosperm influences these traits. If so, whether DNA methylation participated in these processes？ To answer these questions, isolation of pure liquid endosperm qualified for extraction genomic DNA and RNA was the prerequisite. However, it was not easy to conduct this isolation due to endosperm is encapsulated by tiny seed coat and it adjoins to de-veloping embryo. This research adopted a procedure for isolation pure lipid endosperm from 25DAF （days after flowering） ovules of Brassica napus and improved procedure for subsequent DNA extraction. With the optimized procedures, we successfully extracted genomic DNA of liquid endosperm from 500 mg o-vules with high concentration （ ≥2. 5μg/μl, 200μl） and high purity, which were proved by the subse-quent analysis.

关键词： rapeseed liquid endosperm genomic DNA effective

Overexpression of Sddt enhances drought tolerance in Arabidopsis and Brassica napus by regulating micro RNA activity

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Overexpression of Sddt enhances drought tolerance in Arabido...

2016年全国植物生物学大会

作者： Zhiyong Hu Liang Zhang Jing Liu Gaomiao Zhan Hongli Yang Xingchao Sun linbin deng Shihang Fan Hanzhong Wang Wei Hua Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

In plants,high root density and larger root surface area can be maintained or expanded to promote water uptake,a process that plays crucial role in drought stress tolerance. In our study,a gene named Sddt(a semi-dwarfism and drought tolerance gene) was found to regulate the development of lateral root growth in Arabidopsis and Brassica napus. Compared with wild type,transgenic plants overexpressing Sddt showed increased lateral roots. Using yeast-2-hybrid,we demonstrated that Sddt interacted with DCL1. Hybridization of Sddt-overexpressing plants with miRNA activity reporter plants indicated that Sddt greatly enhanced miRNA activity. Furthermore,by small RNA sequencing,we identified 96 known differentially expressed miRNA genes. Among them,the miRNA160 family was up-regulated and its target genes,ARF10,-16,and-17,were down-regulated. Our results suggest that the overexpression of Sddt enhances micro RNA activity via its interaction with DCL1,decreases expression of ARF genes through miRNA160,promoting both lateral root development and increased drought stress tolerance. These findings will help us to better understand the mechanism of root development during drought stress,and implies that Sddt has potential applications in genetic engineering to improve crop tolerance.

关键词： Arabidopsis Brassica napus drought tolerance miRNA activity Sddt miRNA160

The BnGRF2 gene(GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis

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The BnGRF2 gene(GRF2-like gene from Brassica napus) enhances...

第十三届全国植物基因组学大会

作者： Jing Liu~#,Weihua~#,Hongli Yang,Gaomiao Zhan,linbin deng,Rongjun Li,Xinfa Wang, Guihua Liu,Hanzhong Wang~* Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062, China

Seed yield and oil content are two important agricultural characteristics in oil crop breeding,and a lot of functional gene research is being concentrated on increasing these *** this study,by differential gene expression analyses between rapeseed lines(zy036 and 51070) which exhibit different levels of seed oil production,

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拟南芥种子含油量突变体的筛选与相关功能基因鉴定

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氨基酸和生物资源 2016年第4期38卷 20-25页

作者：林久禄刘静魏廷龙杨红丽邓林彬华玮中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室湖北武汉430062 常德市农林科学研究院油料研究所湖南常德415000

我国油菜含油量低,严重影响了国产菜籽的市场竞争力,提高含油量是油菜育种的当务之急。拟南芥与油菜同属十字花科,利用拟南芥筛选油脂合成及调控相关的新基因并将其应用于油菜,是快速选育高油油菜品种的有效手段。本研究采用密度梯度离... 详细信息

我国油菜含油量低,严重影响了国产菜籽的市场竞争力,提高含油量是油菜育种的当务之急。拟南芥与油菜同属十字花科,利用拟南芥筛选油脂合成及调控相关的新基因并将其应用于油菜,是快速选育高油油菜品种的有效手段。本研究采用密度梯度离心方法及核磁共振检测油菜种子的含油量,从6 868份拟南芥突变体库资源中筛选得到9个种子含油量较野生型呈显著性差异的株系。通过T-DNA插入突变的特点,筛选得到6个调控种子油脂合成的候选基因:AT2G19450、AT3G26790、AT3G54320、AT5G06270、AT2G21840和AT3G47450,其中AT5G06270,AT2G21840和AT3G47450属于未知功能基因,为深入研究种子含油量和其调控机理提供了一定的参考。

关键词：拟南芥突变体库含油量功能基因

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异质性国际投资争端与中国对外直接投资

世界经济研究 2021年第4期 88-103,M0004页

作者：袁保生王林彬邓峰新疆大学经济与管理学院新疆大学法学院

已有理论认为投资者—东道国投资争端将导致外商直接投资流入下降,但未考虑国际投资争端适用条约和条款的异质性特征。文章基于1987年以来全球投资争端案例及2003~2017年中国对131个国家对外直接投资(OFDI)面板数据,研究异质性国际投资... 详细信息

已有理论认为投资者—东道国投资争端将导致外商直接投资流入下降,但未考虑国际投资争端适用条约和条款的异质性特征。文章基于1987年以来全球投资争端案例及2003~2017年中国对131个国家对外直接投资(OFDI)面板数据,研究异质性国际投资争端对中国OFDI影响的差异。研究结果表明:国际投资争端数量增加和违约程度提高对中国OFDI具有显著的抑制作用;适用双边投资协定投资争端对中国OFDI的负面影响大于适用含投资条款协定投资争端;投资争端涉嫌违反公正公平、全面保护和间接征收等界定模糊条款的负面效应显著,而涉嫌违反直接征收和资金自由转移等界定明晰条款的影响作用不显著;东道国投资条约数量增加对中国OFDI具有显著促进作用,并在一定程度上缓解了投资争端的负面效应,经济危机时期,救市措施可能导致投资争端的风险预警机制失灵;相比发达国家,发展中国家投资争端对中国OFDI的抑制作用更强。

关键词：国际投资争端国际投资条约中国对外直接投资

双边条约对中国对外直接投资的影响机制研究——基于“一带一路”沿线国家的面板分位数实证分析

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国际商务（对外经济贸易大学学报） 2020年第6期 62-77页

作者：袁保生王林彬邓峰新疆大学经济与管理学院新疆乌鲁木齐830046 新疆大学法学院新疆乌鲁木齐830046

通过构建双边条约对中国对外直接投资影响的理论框架,利用2003~2018年中国与"一带一路"沿线42个国家签订的1 712份双边条约,从时间、空间和条约异质性等角度进行实证检验。研究表明:双边条约对中国对外直接投资的促进作用随投资规模增... 详细信息

通过构建双边条约对中国对外直接投资影响的理论框架,利用2003~2018年中国与"一带一路"沿线42个国家签订的1 712份双边条约,从时间、空间和条约异质性等角度进行实证检验。研究表明:双边条约对中国对外直接投资的促进作用随投资规模增加呈倒U型特征,对出口贸易的"替代"效应随投资规模增加而下降。从区域来看,双边条约显著促进了中国对"丝绸之路经济带"国家的对外直接投资;制度质量越低,双边条约对"21世纪海上丝绸之路"国家对外直接投资的影响作用越强。从条约法律效力看,柔性条约对中国对外直接投资的促进作用大于刚性条约,当宏观经济环境恶化时,制度质量越高柔性条约的作用越强,制度质量越低刚性条约的作用越大。从条约内容看,政治条约对中国对外直接投资的促进作用大于商务和文化条约。

关键词：双边条约对外直接投资面板分位数回归 “一带一路”

基于磁控溅射的Pt/PtRh高温薄膜热电偶传感器集成工艺

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材料工程 2024年第9期52卷 27-33页

作者：张文琦沈统王林斌赵未昀邓元北京航空航天大学杭州创新研究院浙江省智能传感材料与芯片集成技术重点实验室杭州310052 北京航空航天大学前沿科学技术创新研究院北京100083

Pt/PtRh为高温薄膜热电偶敏感材料,Al_(2)O_(3)和SiO_(2)为复合绝缘层材料,采用磁控溅射法成功制备Pt/PtRh薄膜热电偶及Al_(2)O_(3)/SiO_(2)复合型上下绝缘层,并通过掩膜-沉积法实现薄膜热电偶图案化制备。结果表明:制备的复合绝缘层Al_... 详细信息

Pt/PtRh为高温薄膜热电偶敏感材料,Al_(2)O_(3)和SiO_(2)为复合绝缘层材料,采用磁控溅射法成功制备Pt/PtRh薄膜热电偶及Al_(2)O_(3)/SiO_(2)复合型上下绝缘层,并通过掩膜-沉积法实现薄膜热电偶图案化制备。结果表明:制备的复合绝缘层Al_(2)O_(3)/SiO_(2)能够有效保证高温薄膜热电偶传感器在1200℃高温下的绝缘性和化学稳定性。Pt/PtRh热电偶阵列精度达到200μm,可在1200℃下稳定工作超过2200 min,经过4000 min升降温循环测试后,热电偶输出热电动势偏差小于1%。热电偶传感器整体厚度小于20μm,集成于合金与热障涂层之间的热电偶在高温服役环境下可稳定工作并测量合金表面温度。多层结构的样件在服役测试中无分层、脱落现象,热电偶稳定输出信号。

关键词：高温薄膜热电偶铂-铂铑热电偶塞贝克效应氧化铝绝缘层氧化硅绝缘层