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谷子基因组中SSR的偏态分布

华北农学报 2024年第2期39卷 19-26页

作者：高建明邱丽娜桂枝天津农学院农学与资源环境学院天津300384

为了研究谷子基因组中SSR的分布偏好,采用生物信息学方法搜索和分析了谷子基因组中SSR在基因座5′侧翼区、3′侧翼区、外显子区和内含子区的分布规律。结果发现,随着5′侧翼区与起始密码子间距离的增加,SSR在5′侧翼区的频率与其基因组... 详细信息

为了研究谷子基因组中SSR的分布偏好,采用生物信息学方法搜索和分析了谷子基因组中SSR在基因座5′侧翼区、3′侧翼区、外显子区和内含子区的分布规律。结果发现,随着5′侧翼区与起始密码子间距离的增加,SSR在5′侧翼区的频率与其基因组频率的比值(相对频率)逐渐下降,在1~700 bp区域内相对频率远大于1.50,而在3′侧翼区、外显子区和内含子区中SSR的相对频率均接近1.00。进一步分析发现,随着5′侧翼区与起始密码子间距离的增加,不同单一SSR类型的相对频率的变化趋势各不相同,总体上,包括CCG、AG、AGG、AGGG、ACC和AGC在内的这6个SSR类型在5′侧翼区的频率和相对频率均较高,其中,AG在5′侧翼区相对频率大于1.5的区域最大(1~1 800 bp),而在CCG的区域最小(1~200 bp)。在外显子区,CCG、AGG、AGC、ACG和ACC这5个高C/G含量的三碱基SSR类型具有较高的频率和相对频率。综上,SSR在基因座5′侧翼区1~700 bp内特异性出现,其中,包括CCG、AG、AGG、AGGG、ACC和AGC在内的6个SSR类型为主要的特异性分布类型;而在基因座3′侧翼区、外显子区和内含子区中,SSR的分布与整个基因组无明显差别,但在外显子区,CCG、AGG、AGC、ACG和ACC这5个SSR类型具有明显的分布特异性。

关键词：谷子 SSR 分布偏好 5′侧翼区外显子区

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湿法磷酸萃取技术发展现状与研究进展

工程科学学报 2024年第11期46卷 1948-1959页

作者：黄欣雨甘晨张名扬邱丽娜弓爱君董艺北京科技大学化学与生物工程学院北京100083 北京科技大学功能分子与晶态材料科学与应用北京市重点实验室北京100083

磷酸作为一种重要的化工原料,在化工业中占有极其重要的地位.磷酸的应用主要由磷酸的纯度决定,低纯度磷酸可用于工业和农业领域,而高纯度磷酸则可用于医药、食品和电子等行业.我国磷矿以低品位磷矿为主,生产磷酸主要采用湿法工艺.相比... 详细信息

磷酸作为一种重要的化工原料,在化工业中占有极其重要的地位.磷酸的应用主要由磷酸的纯度决定,低纯度磷酸可用于工业和农业领域,而高纯度磷酸则可用于医药、食品和电子等行业.我国磷矿以低品位磷矿为主,生产磷酸主要采用湿法工艺.相比热法生产,湿法工艺更加清洁环保,但产品杂质含量多、种类复杂,故发展磷酸净化技术尤为重要.本文从湿法磷酸纯化技术中的萃取法出发,综述了萃取法的主要研究进展.重点介绍了溶剂萃取法、双水相萃取法、反胶团萃取法、超声协助萃取法和超临界流体萃取法的基本原理和发展趋势.分析了不同萃取方法的优缺点、分离效果和适用条件.突出介绍了溶剂萃取法,梳理了磷酸除杂的主要萃取剂,特别强调了复合萃取剂和新型萃取剂在磷酸纯化方面的显著优势,最后,对磷酸的萃取技术做出了前景展望.

关键词：磷酸湿法磷酸萃取萃取剂双水相萃取反胶团萃取超声协助萃取超临界流体萃取

Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica

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Horticulture Research 2020年第1期7卷 1561-1571页

作者： lina qiu Yongzhang Wang Haiyong Qu College of Horticulture Qingdao Agricultural UniversityQingdao CityChina

Cytosolic Ca^(2+)plays a key role in signal transduction in *** imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca^(2+)***,we used mature‘Fuji’apples(Malus pumila Mill.)to obtain viable protoplasts from flesh tissue cells by enzymatic hydrolysis;then,three small-molecule fluorescent probes(fluo-8/AM,fluo-4/AM,and rhod-2/AM)were loaded into the *** three Ca^(2+)fluorescent probes successfully entered the cytoplasm but did not enter the *** the Ca^(2+)chelator(EGTA)and Ca^(2+)channel blocker(La3+)reduced the fluorescence intensity in the *** calcium ionophore A23187 increased the fluorescence intensity in the cytoplasm at 5μmol/L but decreased it at 50μmol/***,A23187 reversed the fluorescence intensity in the cytoplasm,which was decreased by La3+.Ionomycin is also a calcium ionophore that can increase the fluorescence intensity of calcium in the *** results suggest that small-molecule Ca^(2+)fluorescent probes can be used to detect changes in cytosolic calcium levels in the cells of fruit flesh *** addition,the optimum concentration of fluo-8/AM was determined to be 5μmol/*** was the first time that protoplasts have been isolated from apple flesh tissue cells and small-molecule fluorescent probes have been used to detect calcium in the cytoplasm of flesh tissue *** study provides a new method to study calcium signal transduction in fruit flesh tissue.

关键词： reversed probes hydrolysis

Distribution characteristics and indicator significance of Dechloranes in multi-matrices at Ny-lesund in the Arctic

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Journal of Environmental Sciences 2015年第2期27卷 8-13页

作者： Guangshui Na Wei Wei Shiyao Zhou Hui Gao Xindong Ma lina qiu Linke Ge Chenguang Bao Ziwei Yao Key Laboratory for Ecological Environment in Coastal Areas National Marine Environmental Monitoring Center Research Center for Eco-environmental Sciences Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University Dalian Institute of Science & Technology School of Biological Technology Dalian Polytechnic University

In recent years, Dechloranes have been widely detected in the environment around the ***, understanding and knowledge of Dechloranes in remote regions, such as the Arctic,remain lacking. Therefore, the concentrations of 5 Dechloranes in surface seawater, sediment,soil, moss, and dung collected from Ny-lesund in the Arctic were measured with the concentrations 93 pg/L, 342, 325, 1.4, and 258 pg/g, respectively, which were much lower than those in Asian and European regions. The mean ratios of anti-Dechlorane Plus（DP） to total DP（fanti） in seawater, sediment, soil, moss, dung, and atmospheric samples were 0.36, 0.21, 0.18,0.27, 0.66, and 0.43, respectively. Results suggested that the main source of DP in seawater,sediment, soil, and moss was long-range atmospheric transport. However, the ratio identified in dung was different, for which the migration behavior of the organism is probably the main source of DP.

关键词： Dechloranes Arctic Multi-matrices Distribution characteristic Ratio Source analysis

小麦WRKY转录因子TaWRKY72B的克隆、亚细胞定位及表达分析

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麦类作物学报 2024年第1期44卷 7-15页

作者：张丽甘露卢振华曹高燚邱丽娜丁博谢晓东秦志列王俊斌天津农学院天津市作物抗逆的机理及遗传改良国际联合研究中心天津300392 北京市农林科学院杂交小麦研究所北京100097 天津农学院基础科学学院天津300392

WRKY蛋白是一类转录因子,参与调控植物的多种生长发育和胁迫应答过程。为进一步探究WRKY在小麦响应逆境中的作用,本研究克隆了小麦中国春的TaWRKY72B基因,并对其进行了生物信息学、亚细胞定位和表达模式分析。结果表明,中国春的TaWRKY72... 详细信息

WRKY蛋白是一类转录因子,参与调控植物的多种生长发育和胁迫应答过程。为进一步探究WRKY在小麦响应逆境中的作用,本研究克隆了小麦中国春的TaWRKY72B基因,并对其进行了生物信息学、亚细胞定位和表达模式分析。结果表明,中国春的TaWRKY72B基因编码320个氨基酸,蛋白序列包含典型的WRKY保守结构域和C2H2锌指结构,分子量为33.94 kDa,理论等电点为6.60,是酸性亲水的不稳定蛋白,无信号肽序列,属非跨膜蛋白。二级结构预测表明,TaWRKY72B主要由无规则卷曲(61.25%)、α-螺旋(23.12%)、延伸链(11.88%)和β-转角(3.75%)组成。TaWRKY72B定位于细胞核,符合转录因子特征。系统进化树分析显示,TaWRKY72B蛋白与节节麦、大麦和水稻等禾本科作物的WRKY亲缘关系较近。启动子顺式作用元件预测结果表明,该基因含有多个与生长发育、激素信号途径以及非生物胁迫应答相关的顺式调控元件。TaWRKY72B表达受激素、高温、低温和盐诱导,推测该基因可能参与小麦逆境胁迫应答和多种激素信号途径。

关键词：小麦 WRKY转录因子基因克隆亚细胞定位表达分析

MABR-AO耦合工艺处理乡镇低C/N比生活污水效果研究

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环境影响评价 2024年第3期46卷 69-73,77页

作者：胡艺维何勇宋南川牛佳伟杨程邱丽娜重庆交通大学河海学院重庆400074 重庆市合川区生态环境监测站重庆401520 重庆市豪洋水务建设管理有限公司重庆400020 重庆市科学技术研究院低碳与生态环保研究中心重庆401121

本研究针对村镇生活污水低碳氮比导致碳源不足、硝化-反硝化工艺动能不足、脱氮难度高导致脱氮效果不理想的问题,通过设计搭建膜曝气生物反应器(MABR)与传统AO工艺的耦合工艺系统,对村镇进水条件下的挂膜启动,处理低碳氮比(COD/TN为4、3... 详细信息

本研究针对村镇生活污水低碳氮比导致碳源不足、硝化-反硝化工艺动能不足、脱氮难度高导致脱氮效果不理想的问题,通过设计搭建膜曝气生物反应器(MABR)与传统AO工艺的耦合工艺系统,对村镇进水条件下的挂膜启动,处理低碳氮比(COD/TN为4、3、2、1、0.5)污水,并对两种活性污泥的生物多样性检测分析。结果表明,MABR挂膜周期约30天,系统达到较为稳定的污染物去除效果;在低碳氮比进水条件下,MABR-AO耦合工艺比AO工艺有更好的脱氮能力,总氮(TN)平均去除率相比AO工艺提高17.9%;MABR生物膜中OUTs更高,且脱氮类微生物占比高于AO工艺活性污泥,是脱氮能力更强的主要原因。该工艺运维简单,低碳氮比条件下总氮去除能力较强。

关键词： MABR-AO耦合工艺乡镇污水低碳氮比

Extraction and back-extraction behaviors of 14 rare earth elements from sulfuric acid medium by TODGA

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Journal of Rare Earths 2018年第6期36卷 642-647页

作者： Huiting Yuan Weixiang Hong Yushan Zhou Baisong Pu Aijun Gong Tao Xu Qishan Yang Fukai Li lina qiu Weiwei Zhang Yuning Liu School of Chemistry and Biological Engineering University of Science and Technology BeijingBeijing 100083China North China Rare Earth Hi-Tech Company Limited Baotou 014030China School of Chemistry and Chemical Engineering Inner Mangolia University of Science and TechnologyBaotou 014010China

The unique physical and chemical properties of rare earth elements lay the foundation for their extensive application. N,N,N’,N’ Tetra-octyl-3-oxopentanediamide(TODGA) is excellent in its ability of extracting rare earth elements and it is favored for green initiative. In this paper, the extraction and back-extraction of14 rare earth elements by TODGA were studied. Experiments show that in conditions of 6 mol/L sulfuric acid, the extraction temperature of 25 ℃,the phase ratio of 1:1 and 0.04 mol/LTODGA(aviation kerosene as the diluent), the extraction rates of 14 rare earth elements including lanthanum, cerium, praseodymium,neodymium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium were 99.00%-99.73%. Mixed with hydrochloric acid and nitric acid(HCl 3.5 mol/L, HNO30.5 mol/L), the recoveries of the 14 rare earth elements are 91.52%-99.91% when the extraction temperature is 25 ℃ and the ratio is 1:1. The following application is based on the optimum conditions above with practical samples(from the roasting production line of China North Rare Earth High-tech Company Limited) for extraction and back-extraction experiments. Experiments show that TODGA has excellent enrichment effect on 14 rare earth elements, the extraction rates are 91.36%-99.80%, the back-extraction rates are 87.29%-99.64% and the total recoveries are 81.19%-99.44%.

关键词： Rare earth Extraction Back-extraction N,N,N',N' Tetra-octyl-3-oxopentanediamide(TODGA) Enrichment

Research progress of rare earth separation methods and technologies

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Journal of Rare Earths 2023年第2期41卷 182-189页

作者： Mory Traore Aijun Gong Yiwen Wang lina qiu Yuzhen Bai Weiyu Zhao Yang Liu Yi Chen Ying Liu Huilin Wu Shuli Li Yueyi You School of Chemistry and Biological Engineering University of Science and Technology BeijingBeijing 100083China b Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials University of Science and Technology BeijingBeijing 100083China

Strategical elements,such as rare earth elements,play a crucial role in the industry,especially in producing high-tech *** global industries have developed a strong dependence on rare earth *** year,there are innovations in industries such as modern technology,green energy,or communications technology,which need more strategic metals to improve investment *** article reviews advances in rare earth separation methods and techniques to guide and recommend the best extractants,depending on leaching conditions and the final target *** method for separating and extracting agents is individually revised in terms of the mechanism and interaction of providing rare earth *** paper also evaluates past and current trends of these methods and technical extractants and identifies their strengths and weaknesses.

关键词： Rare earth Separation Extraction Extractant

夯实“川东粮仓”视域下撂荒地整治现状与治理对策

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粮食科技与经济 2024年第1期49卷 32-37页

作者：周继芬任厚洪刘宗川赵小婧陈琦董凌伯邱利娜达州职业技术学院四川达州635001 达州市茶果站四川达州635000

通过大量实地走访、调查,得到达州市土地撂荒主要原因是山地多,交通条件差,气候条件恶劣,种植效益低,搬迁新居、外出务工等流转限制因子多,建设、工业征地项目暂未完成,动物啃食植物导致无收成等;复耕意愿不强,资金和人力支持不够,技术... 详细信息

通过大量实地走访、调查,得到达州市土地撂荒主要原因是山地多,交通条件差,气候条件恶劣,种植效益低,搬迁新居、外出务工等流转限制因子多,建设、工业征地项目暂未完成,动物啃食植物导致无收成等;复耕意愿不强,资金和人力支持不够,技术薄弱,机械化程度不够等诸多问题。建议通过加强督促政府部门提高认识,把粮食安全问题的落实及完善作为党政领导的目标绩效考核内容。因地制宜,宜粮则粮,宜经则经,建立森林粮库。改善交通、发展小型农机具,实现机械化耕种;筹建高标准农田,提高种植效益;针对“假种地”恶意套取国家资金行为,实行零容忍处理;引进新型合作企业和发展村集体复耕;提交农村医疗和教育质量等一系列综合治理措施,真正实现国家藏粮于地粮食安全策略。

关键词：耕地撂荒原因对策