检索结果-南通市图书馆

Subculturing cells have no effect on CRISPR/Cas9-mediated cleavage of UL30 gene in pseudorabies virus

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2018年第1期1卷 74-77页

作者： lin-zhu ren Zhi-yuan Peng Ting Ouyang Xiao-hui Liu Xin-rong Chen Li Ye Jun-wen Fan Hong-sheng Ouyang Da-xin Pang Jie-ying Bai Jilin Provincial Key Laboratory of Animal Embryo Engineering College of Animal Sciences Jilin University The Laboratory Animal Center The Academic of Military Medical Sciences

CRISPR/Cas9-mediated genome editing can inhibit virus infection by targeting the conserved regions of the viral genomic DNA. Unexpectedly, we found previously that pseudorabies virus(PRV) could escape from CRISPR/Cas9-mediated *** order to elucidate whether the escape of PRV from Cas9-mediated inhibition was due to cell deficiencies, such as genetic instability of sgRNA or Cas9 protein, the positive cells were passaged ten times, and PRV infection in the sgRNA-expressing cells was evaluated in the present study. The results showed that subculturing cells has no effect on Cas9-mediated cleavage of PRV. Different passages of PX459-PRV cells can stably express sgRNA to facilitate Cas9/sgRNA cleavage on the UL30 gene of PRV, resulting in a pronounced inhibition of PRV infection. Studies to elucidate the mechanism of PRV escape are currently in progress.

关键词： CRISPR/Cas9 pseudorabies virus(PRV) single-guide RNA(sgRNA) UL30 protein

Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： Xiao Xiang Feng Gao Yu-Gui Peng Peng Wu Zong-lin Li Xue-Feng zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

投影寻踪聚类在新疆地震预报中的应用

内陆地震 2005年第1期19卷 8-15页

作者：王琼朱令人新疆维吾尔自治区地震局新疆乌鲁木齐830011

投影寻踪聚类(PPC)是投影寻踪和非分层聚类方法的综合,它同时尝试分类数据和寻找聚类结构的低维有代表性的特征。对于本项研究,***.B提出一个基于正交标准变量(考虑样本的大小)的投影寻踪指数和判断投影寻踪聚类的最优类别数的方法对新... 详细信息

投影寻踪聚类(PPC)是投影寻踪和非分层聚类方法的综合,它同时尝试分类数据和寻找聚类结构的低维有代表性的特征。对于本项研究,***.B提出一个基于正交标准变量(考虑样本的大小)的投影寻踪指数和判断投影寻踪聚类的最优类别数的方法对新疆地区地震活动作聚类分析,模拟聚类结构。找出不同区域的有效的参量组合结构,判断后续聚类结构,对未来地震大小做出预测。通过对由不同的时间窗长和滑动步长的地震活动性自变量的PP聚类分析,发现短期预测效果不好,而时间窗长分别为12个月和18个月,相应的步长为6个月和12个月的PP聚类效果较好:柯坪地区、乌恰地区的投影寻踪聚类分析的内符检验对应率达60%以上。通过对比分析,认为取时间窗长为18个月的结果更好些。这与自变量的选取有关——地震活动性参量主要是反映中期震兆信息。

关键词：投影寻踪聚类分析投影寻踪聚类新疆地区

Non-coding RNAs in acute ischemic stroke:from brain to periphery

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan Qin lin zhu Teng Jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

Gas-Solid Flow,Combustion Characteristics,and Gas Emissions in a 75 t/h CFB Boiler Based on the CPFD Method

在线全文

学校读者我要写书评

暂无评论

Journal of Thermal Science 2025年第1期34卷 323-336页

作者： HUANG Xiankun BAI Zhang zhu Xiaoli WANG Shuoshuo MU lin GONG Liang College of New Energy China University of Petroleum (East China) Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education School of Energy and Power EngineeringDalian University of Technology

A 3D simulation using Computational Particle Fluid Dynamics（CPFD） methods was used to calculate coal combustion in a 75 t/h industrial-scale circulating fluidized bed（CFB） *** characteristics,gas-solid flow characteristics,and gaseous pollutant emissions of CFB boilers from combustion ignition to stable operation were systematically evaluated in this *** show that the temperature distribution is relatively uniform throughout the *** the combustion process unfolds within the boiler,the gas composition curve strikingly portrays the inverse correlation between CO2and *** the combustion reaction progresses,it becomes evident that the concentration of CO2progressively increases,while the concentration of O2concurrently *** inverse relationship underscores the fundamental combustion reaction,where carbon-based fuels react with oxygen to produce carbon dioxide and release ***,a comprehensive analysis has revealed that,from ignition to stable combustion,both nitric oxide（NO） and sulfur dioxide（SO2） emissions exhibit a declining *** reduction in pollutant generation is attributed to the improvement in combustion *** complete combustion leads to lower levels of unburned hydrocarbons,which are prone to NO ***,the sulfur content in the fuel is more efficiently oxidized to sulfur trioxide（SO3） or bound in sulfates,reducing *** steady state in the simulation,the SO2mass flow rate varies significantly with the furnace height,gradually increasing from 0.07 kg·s-1at 4 m at the bottom of the furnace to a peak of 0.078 kg·s-1at 8 m in the center,and then decreasing to 0.06 kg·s-1at 20m at the top of the furnace.

关键词： Sulfur dioxide

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： lin Yin Yalan Xu Jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

Sulfolane‑Based Flame‑Retardant Electrolyte for High‑Voltage Sodium‑Ion Batteries

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第2期17卷 498-516页

作者： Xuanlong He Jie Peng Qingyun lin Meng Li Weibin Chen Pei Liu Tao Huang Zhencheng Huang Yuying Liu Jiaojiao Deng Shenghua Ye Xuming Yang Xiangzhong ren Xiaoping Ouyang Jianhong Liu Biwei Xiao Jiangtao Hu Qianling Zhang Graphene Composite Research Center College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Center of Electron Microscopy State Key Laboratory of Silicon and Advanced Semiconductor MaterialsSchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China GRINM(Guangdong)Research Institute for Advanced Materials and Technology FoshanGuangdong 528051People’s Republic of China College of Energy Engineering Zhejiang UniversityHangzhouZhejiang 310027People’s Republic of China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105People’s Republic of China Shenzhen Eigen-Equation Graphene Technology Co.Ltd Shenzhen 518000People’s Republic of China

Sodium-ion batteries hold great promise as next-generation energy storage ***,the high instability of the electrode/electrolyte interphase during cycling has seriously hindered the development of *** particular,an unstable cathode–electrolyte interphase(CEI)leads to successive electrolyte side reactions,transition metal leaching and rapid capacity decay,which tends to be exacerbated under high-voltage ***,constructing dense and stable CEIs are crucial for high-performance *** work reports localized high-concentration electrolyte by incorporating a highly oxidation-resistant sulfolane solvent with non-solvent diluent 1H,1H,5H-octafluoropentyl-1,1,2,2-tetrafluoroethyl ether,which exhibited excellent oxidative stability and was able to form thin,dense and homogeneous *** excellent CEI enabled the O3-type layered oxide cathode NaNi_(1/3)Mn_(1/3)Fe_(1/3)O_(2)(NaNMF)to achieve stable cycling,with a capacity retention of 79.48%after 300 cycles at 1 C and 81.15%after 400 cycles at 2 C with a high charging voltage of 4.2 *** addition,its nonflammable nature enhances the safety of *** work provides a viable pathway for the application of sulfolane-based electrolytes on SIBs and the design of next-generation high-voltage electrolytes.

关键词： Sodium-ion batteries Sulfolane-based electrolyte High voltage Layered oxide cathode Flame retardant

在线全文

学校读者我要写书评

暂无评论

不同间距对旁靠浮式系统的影响

武汉船舶职业技术学院学报 2018年第3期17卷 18-23页

作者：林文朱仁庆韩峥孙宝瑞江苏科技大学船舶与海洋工程学院江苏镇江212003

在两旁靠浮体水动力性能研究中,我们将面临着不同的间距对两旁靠浮体产生的影响问题。本文基于粘流理论,利用STARCCM+软件对不同间距情况下的两浮体六自由度的运动、系泊缆绳的受力以及波面的升高情况进行对比分析。分析中我们可以发现... 详细信息

在两旁靠浮体水动力性能研究中,我们将面临着不同的间距对两旁靠浮体产生的影响问题。本文基于粘流理论,利用STARCCM+软件对不同间距情况下的两浮体六自由度的运动、系泊缆绳的受力以及波面的升高情况进行对比分析。分析中我们可以发现不同的间距对两旁靠浮体的影响是不可忽视的,其中,间距越小,影响越剧烈。

关键词：旁靠浮体粘流理论 STARCCM+ 波面升高

在线全文

学校读者我要写书评

暂无评论

基于学习的证券市场专家预测意见合成研究

系统工程学报 2004年第1期19卷 94-98页

作者：杨善林朱卫东任明仑合肥工业大学管理学院安徽合肥230009

针对证券市场的专家预测意见的特点,研究了将学习、证据理论与协同学理论引入证券市场专家预测意见合成的理论与方法.讨论了将专家的历史预测数据用基本可信数表示作为学习样本,对初选专家进行聚类分析选择有协同效应的专家.该方法用神... 详细信息

针对证券市场的专家预测意见的特点,研究了将学习、证据理论与协同学理论引入证券市场专家预测意见合成的理论与方法.讨论了将专家的历史预测数据用基本可信数表示作为学习样本,对初选专家进行聚类分析选择有协同效应的专家.该方法用神经网络优化基本可信数的修正系数,再用Dempster合成规则进行预测意见的合成.实验结果表明该方法应用于解决实际问题时具有较好的效果.

关键词：证券市场证据理论神经网络证券投资专家预测意见合成聚类分析