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Biological impacts of "hot-spot" mutations of hepatitis B virus X proteins are genotype B and C differentiated

World Journal of Gastroenterology 2005年第30期11卷 4703-4708页

作者： Xu lin Xiao Xu qing-ling huang Yu-qing Liu Da-Li Zheng Wan-Nan Chen Jian-Yin lin Research Center of Molecular Medicine Fujian Medical University Fuzhou 350004 Fujian Province China Centre for the Study of Liver Disease and Department of Surgery The University of Hong Kong Pokfulam Hong Kong China

AIM: To investigate the biological impacts of 'hot-spot'mutations on genotype B and C HBV X proteins (HBx).METHODS: Five types of'hot-spot' mutations of genotype B or C HBV X genes, which sequentially lead to the amino acid substitutions of HBx as I127T, F132Y, K130M+V131I,I127T+K130M+V131I, or K130M+V131I+F132Y, respectively,were generated by means of site-directed *** evaluate the anti-proliferative effects, HBx or related mutants' expression vectors were transfected separately to the Chang cells by lipofectamine, and the cells were cultured in hygromycin selective medium for 14 d, drug-resistant colonies were fixed with cold methanol, stained with Giemsa dyes and scored (increase of the colonies indicated the reduction of the anti-proliferation activity,and vice versa). Different types of HBx expression vectors were co-transfected separately with the reporter plasmid pCMVβ to Chang cells, which were lysed 48 h post-transfection and the intra-cellular β-galactosidase activities were monitored (increase of the β-galactosidase activities indicated the reduction of the transactivation activity, and vice versa). All data obtained were calculated by paired-samples ***: As compared to standard genotype B HBx,mutants of I127T and I127T+K130M+V131I showed higher transactivation and anti-proliferative activities, while the mutants of F132Y, K130M+V131I, and K130M+V131I+F132Y showed lower activities. As compared to standard genotype C HBx, I127T mutant showed higher transactivation activity, while the other four types of mutants showed no differences. With regard to anti-proliferative activity,compared to standard genotype C HBx, F132Y and K130M+V131I mutants showed lower activities, and K130M+V131I +F132Y mutant, on the other hand, showed higher activity,while the mutants of I127T and I127T+K130M+V131I showed no ***: 'Hot-spot' mutations affect the antiproliferation and transactivation activities of genotype B and/or C HBx, and the

关键词： Hepatitis B virus Genotype X gene Mutation

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XRD Analysis on the Fluorescence Material of Sm Doped Si-Ca-Mg System

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Chinese Journal of Structural Chemistry 2007年第8期26卷 902-906页

作者： huang qing-Ming Instrumentation Analysis and Measurement Center of Fuzhou University Fuzhou Fujian 350002 China

Fluorescence material of Sm doped Si-Ca-Mg system was synthesized by using the method of solid phase reaction at high temperature. The phase composition and crystal structure of this material were analyzed with XRD method for its composition and the existence form of Sm atom. We aimed to exactly determine the phase composition of this fluorescence material and the doping position and environment of rare-earth Sm atom in the system because these factors have significant effects on the properties. The analytical results show that the Sm atoms dope in Ca2O26Si6Sm8 lattice in the form of atomic site-occupation in three different space positions with different occupancy rates. Therefore, based on the XRD analytical results, the fluorescence material of Sm doped Si-Ca-Mg system with high performance can be synthesized.

关键词： XRD fluorescence material Sm structure

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In situ chemical oxidation of aniline by persulfate with iron(Ⅱ) activation at ambient temperature

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Chinese Chemical Letters 2010年第8期21卷 911-913页

作者： Yong qing Zhang Wei lin huang Donna E.Fennell College of Environmental Science and Engineering South China University of TechnologyGuangzhou 510006China The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of EducationGuangzhou 510006China Department of Environmental Sciences RutgersThe State University of New JerseyNew BrunswickNJ 08901USA

The kinetics of aniline degradation by persulfate processes with iron（Ⅱ） activation at ambient temperature was investigated in this *** iron（Ⅱ） as initiator,the oxidation reactions were found to follow a biphasic rate phenomenon：a rapid transformation followed by a slow but sustained oxidation *** the first 30 s,the reaction mainly relies on the persulfate-Fe^（2＋） reaction in which aniline is oxidized *** 30 s,the aniline was still oxidized but the rate of reaction tended to be slower and the rates were clearly *** the initial fast oxidation,the reactions appeared to follow a pseudo-first-order model.

关键词： Persulfate Aniline In situ chemical oxidation

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Study on the Performance of the GRANDProto300 Particle Detector Array by Simulation

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Research in Astronomy and Astrophysics 2024年第1期24卷 173-177页

作者： Fu-lin Dai Quan-Bu Gou Xiaoyuan huang Yi-qing Guo Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China

The Giant Radio Array for Neutrino Detection(GRAND)is a proposed large-scale observatory designed to detect cosmic rays,gamma-rays,and neutrinos with energies exceeding 100 Pe *** GRANDProto300 experiment is proposed as the early stage of the GRAND project,consisting of a hybrid array of radio antennas and scintillator *** latter,as a mature and traditional detector,is used to cross-check the nature of the candidate events selected from radio *** this study,we developed a simulation software called G4GRANDProto300,based on the Geant4 software package,to optimize the spacing of the scintillator detector array and to investigate its effective *** analysis was conducted at various zenith angles under different detector spacings,including 300,500,600,700,and 900 *** results indicate that,for large zenith angles used to search for cosmic-ray in the GRAND project,the optimized effective area is with a detector spacing of 500 *** G4GRANDProto300 software that we developed could be used to further optimize the layout of the particle detector array in future work.

关键词： astroparticle physics instrumentation:detectors (ISM:)cosmic rays

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Immuno-Protective Efficiency of the Bivalent Inactivated Vaccine Against Vibrio scophthalmi and Aeromonas salmonicida Infections in Turbot(Scophthalmus maximus L.)

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Journal of Ocean University of China 2023年第4期22卷 1079-1086页

作者： ZHOU Shun ZHENG Xujia DING Yuanyuan SU lin huang qing XIU Yunji School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China

Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic ***,vaccination is the most promising method for preventing communicable *** this study,we used formalin to kill *** and *** cells,and mixed with the mineralized oil adjuvant(Montanide^(TM)ISA 763 AVG)to prepare the bivalent inactivated *** results showed that turbot inoculated with the bivalent inactivated vaccine exhibited strong tolerance to the infection of *** and ***,and no obvious clinical symptoms and pathological changes were *** activities of enzymes lysozyme,acid phosphatase and complement C3 had significantly increased after the *** antibody titer response of vaccinated turbot was greatly boosted,which was positively connected with the immunological impact according to ELISA ***,the expression levels of immune-related genes such as MHC-IIα,MHC-IIβ,CD4,CD8,TNF-αand IL^(-1)βwere up-regulated,demonstrating that it might stimulate humoral and cellular immunological response in *** findings highlight the potential of the bivalent inactivated vaccine for controlling *** and *** infections in turbot.

关键词： Aeromonas salmonicida Vibrio scophthalmi bivalent inactivated vaccine immune response

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载药外泌体在中枢神经系统疾病中的热点问题

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中国组织工程研究 2025年第23期29卷 5013-5021页

作者：林慧洁黄云黄志华江丽霞赣南医科大学第一临床医学院江西省赣州市341000 赣南医科大学心脑血管重点实验室江西省赣州市341000 赣南医科大学基础医学院江西省赣州市341000 赣南医科大学第一附属医院检验科江西省赣州市341000

背景:利用外泌体作为药物载体不仅可以精确靶向治疗部位,还能提高局部浓度,为药物进入中枢神经系统开辟了一条新途径。目的:探讨外泌体的生物发生、生物学功能,综述当前有关细胞外囊泡作为药物载体在中枢神经系统疾病治疗中的最新进展... 详细信息

背景:利用外泌体作为药物载体不仅可以精确靶向治疗部位,还能提高局部浓度,为药物进入中枢神经系统开辟了一条新途径。目的:探讨外泌体的生物发生、生物学功能,综述当前有关细胞外囊泡作为药物载体在中枢神经系统疾病治疗中的最新进展。方法:由第一作者检索Web of Science、Pub Med和中国知网数据库1976年1月至2024年1月发表的相关文献,以“exosomes,extracellular vesicles,central nervous system,drug delivery,ischemic stroke,Alzheimer’s disease,Parkinson’s disease,spinal cord injury,brain tumor”为英文检索词,以“外泌体,细胞外囊泡,中枢神经系统疾病,载药,脑卒中,阿尔兹海默病,帕金森病,脊髓损伤、脑肿瘤”为中文检索词,最终纳入94篇文献进行分析。结果与结论:(1)外泌体可以轻易通过血脑屏障输送蛋白质、代谢物和核酸到受体细胞中,调节细胞代谢。由于外泌体是由细胞分泌的小囊泡,具有更低的循环免疫原性,在体内循环中能够更少被巨噬细胞识别清除。(2)外泌体可以被设计为递送不同的治疗成分,包括RNA、蛋白质、化疗药物和免疫调节剂,能够将治疗成分传送至期望的目标。经过工程修饰的外泌体具有更好的靶向性,并且这种外泌体介导的传递免疫原性极低,有望为将来中枢神经系统疾病的精准治疗提供更加安全有效的方法。

关键词：中枢神经系统疾病外泌体药物递送脑卒中阿尔茨海默病帕金森病脊髓损伤脑肿瘤

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QE-integrating framework based on Github knowledge and SVM ranking

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Science China(Information Sciences) 2019年第5期62卷 92-107页

作者： qing huang Huaiguang WU School of Computer and Information Engineering Jiangxi Normal University School of Computer and Communication Engineering Zhengzhou University of Light Industry

The latest query expansion(QE) methods use the software development features for expanding queries. However, these methods allow only one feature to be considered at a time. To consider additional features simultaneously, we propose a QE method based on Github knowledge; this is a new comprehensive feature that covers both the existing features(i.e., the application program interface(API) information and crowd knowledge). It is extracted from the "pull requests" of code repositories on Github, which contain descriptions of a request and its commits, the participants’ comments and the API information of the changed files. In addition, we implement a black-box framework that integrates multiple QE methods based on the support vector machine ranking called Github knowledge search repository(GKSR). Our empirical evaluation shows that the GKSR outperforms the state-of-the-art QE methods CodeHow and QECK by 25%–32% in terms of precision.

关键词： code search query expansion Github knowledge SVM ranking crowd knowledge

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Simulation and Experimental Research on Liquid Spreading in a Wire-Sawn Kerf

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Chinese Journal of Mechanical Engineering 2023年第6期36卷 247-260页

作者： lin lin Hui huang State Key Institute of Manufacturing Engineering Huaqiao UniversityXiamen 361021China

The significance of liquids in abrasive wire sawing has been demonstrated in several ***,the perfor-mance of its spreading behavior is limited by the current development trend,where the wafer has a larger area and the kerf is ***,there are very few studies on the liquid spreading behavior in wire-sawn ***,a 3D CFD(computational fluid dynamics)model is presented in this paper and used to simulate the liquid spreading behavior in a kerf based on a VOF(volume of fluid)method with a CSF(continuum surface force)model,which is used to simulate multiphase flow,and an empirical correlation for characterizing the liquid dynamic contact angle using UDF(user defined functions).Subsequently,parametric simulations are performed on the kerf area,kerf width,liquid viscosity,liquid surface tension,and liquid velocity at the inlet area of the kerf,and verification experi-ments are conducted to determine the validity of the simulation *** the simulation and experimental results,three typical liquid spreading regimes that exhibit different effects on wire sawing in the kerfs are found,and their limiting conditions are identified using non-dimensional ***,a prediction model is pro-posed for the liquid spreading regime based on a set of Weber and Capillary *** wire sawing,an increase in the wafer area does not change the liquid spreading regime in the kerf;however,a reduction in the kerf width sig-nificantly hinders the liquid spreading ***,the spreading regime can be effectively converted to facili-tate wire sawing by adjusting the physical properties and supply conditions of the liquid.

关键词： Spreading regime Kerf CFD Non-dimensional analysis Prediction model

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Realization of Bipartite Positive-Operator-Value Measurements of Two-Photon Polarization States

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Communications in Theoretical Physics 2009年第2期51卷 244-246页

作者： lin qing College of Information Science and Engineering Huaqiao University Quanzhou 362021 China

In this letter, we propose a scheme of a special quantum optical Fredkin gate assisted by optical manip- ulations and postselection from the coincidence measurements, and then modify it with cross-Kerr nonlinearities to be suitable for the realization of all possible positive operator-valued measurements of bipartite polarization states. This scheme is feasible in the lab with the current experimental technology.

关键词： POVM linear optics cross-Kerr nonlinearity

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Diazoxide attenuates ischemia/reperfusion injuryvia upregulation of heme oxygenase-1 after liver transplantation in rats

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World Journal of Gastroenterology 2012年第15期18卷 1765-1772页

作者： Zhong Zeng Han-Fei huang Fei He lin-Xi Wu Jie lin Ming-qing Chen Organ Transplantation Center the First Affili-ated Hospital of Kunming Medical CollegeKunming 650032Yunnan ProvinceChina

AIM:To evaluate the effects of diazoxide on ischemia/reperfusion(I/R)-injured hepatocytes and further elucidate its underlying ***:Male Sprague-Dawley rats were randomized(8 for donor and recipient per group)into five groups:I/R group(4 h of liver cold ischemia followed by 6 h of reperfusion);heme oxygenase-1(HO-1)small interfering RNA(siRNA)group(injection of siRNA via donor portal vein 48 h prior to harvest);diazoxide(DZ) group(injection of DZ via donor portal vein 10 min prior to harvest);HO-1 siRNA+DZ group;and siRNA control *** and liver samples were collected at 6 h after *** mRNA expressions and protein levels of HO-1 were determined by reverse transcription polymerase chain reaction and Western blotting,and tissue morphology was examined by light and transmission electron *** transaminases level and cytokines concentration were also ***:We observed that a significant reduction of HO-1 mRNA and protein levels in HO-1 siRNA and HO-1 siRNA+DZ group when compared with I/R group,while the increases were prominent in the DZ *** and transmission electron microscopy indicated severe disruption of tissue with lobular distortion and mitochondrial cristae damage in the HO-1 siRNA and HO-1 siRNA+DZ groups compared with DZ *** alanine aminotransferase,aspartate transaminase,tumor necrosis factor-αand interleukin-6 levels increased in the HO-1 siRNA and HO-1 siRNA+DZ groups,and decreased in the DZ ***:The protective effect of DZ may be induced by upregulation of *** inhibiting expression of HO-1,this protection pretreated with DZ was abolished.

关键词： Ischemia/reperfusion injury Diazoxide Heme oxygenase-1 Liver transplantation Rat

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