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Transferrin and Its Isoforms from Normal Human Serum Revealed by Several Analytical Techniques

Chemical Research in Chinese Universities 2008年第1期24卷 84-91页

作者： ZHUO Hui-qin huang He-qing JIN Hong-wei huang lin Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering School of Life Sciences State Key Laboratory of Marine Environmental Science College of Oceanography and Environmental Science The Key Laboratory of Chemical Biology of Fujian Province College of Chemistry ＆ Chemical Engineering Xiamen Center for Clinical Laboratory Zhongshan Hospital Xiamen University Xiamen 361005 P. R. China

Transferrin（TF） and its isoforms have been widely reported via various analytical techniques, including a noticeable increased number of isoforms with low content of sialic acid（asialo-, monosialo-, and disialo-transferrin） and asialo-TF as well as disialo-TF, with one or several oligosaccharides released in human serum transferrin（hTf）. Here, hTf has been purified by native gradient polyacrylamide gel electrophoresis（PAGEso） before use. The hTf extracted with the electron-transfer approach showed a single subunit band（77.1 Da） in the SDS-PAGE gel, but it exhibited two bands in the native and denatured isoelectric focusing（IEF） gels, namely, hTf-2Fe^3＋ and apo-hTf, without finding any other transferrin isoforms. A reversed phase HPLC（RP-HPLC） equipped with a C18 column effectively separated hTf and its polymers and combined off-line techniques, including peptide mass fingerprinting（PMF）, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and database search, and identified the high homology among hTf, apo-hTf, and their isoforms. Moreover, the elution solution consisting of acetonitrile and formic acid could easily denature both hTf and apo-hTf to form various isoforms during separation with HPLC, indicating that chemical factors lead to the formation of various isoforms in transferrin, artificially, during extraction and separation. The authors claimed that only two transferrin isoforms existed in the NHS, namely, hTf-2Fe^3＋ and apo-hTf, which could be employed in biomarkers, to distinguish the healthy population from many disease sufferers, such as, carbohydrate-deficient transferrin（CDT）

关键词： Human serum transferrin RP-HPLC MALDI-TOF-MS PMF Biomarker

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Plasmon-enhanced broad-band quantum-cutting of NaBaPO4:Eu^2+,Er^3+phosphors with silver nano-particles

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Journal of Rare Earths 2020年第11期38卷 1151-1157,I0001页

作者： Jinquan Hong lin lin Xiaoyan Li Zhuohong Feng Lili huang qing Qin Zhiqiang Zheng College of Physics and Energy Fujian Normal UniversityFuzhou 350117China Department of Physics and Electronic Information Engineering Minjiang UniversityFuzhou 350121China Fujian Provincial Collaborative Innovation Center for Optoelectronic Semiconductors and Efficient Devices Xiamen 361005China

NaBaPO4:Eu^2+,Er^3+phosphors and Ag nano-particles(NPs)were prepared by the solid-state reaction and chemical reduction method,*** fluorescence spectra and decay curves demonstrate the effective energy transfer from Eu^2+to Er^3+and the existence of three-photon quantum-cutting through two-step cross-relaxation of Er^3+.The quantum-cutting emission is peaked at 1534 nm with a broad excitation band centered at 352 nm,Plasmon-enhanced quantum-cutting of NaBaPO4:Eu^2+,Er^3+phosphors was realized by decorating Ag *** largest enhancement factor is *** is hopeful to improve the photovoltaic conversion efficiency of Ge solar cells by using this phosphor.

关键词： Luminescence Localized surface plasmon resonance Broad-band quantum-cutting Rare-earth doped phosphors

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Multiple multicontrol unitary operations: Implementation and applications

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Science China(Physics,Mechanics & Astronomy) 2018年第4期61卷 44-53页

作者： qing lin Fujian Key Laboratory of Light Propagation and Transformation College of Information Science and EngineeringHuaqiao University

The efficient implementation of computational tasks is critical to quantum computations. In quantum circuits, multicontrol unitary operations are important components. Here, we present an extremely efficient and direct approach to multiple multicontrol unitary operations without decomposition to CNOT and single-photon gates. With the proposed approach, the necessary twophoton operations could be reduced from O(n^3) with the traditional decomposition approach to O(n), which will greatly relax the requirements and make large-scale quantum computation feasible. Moreover, we propose the potential application to the(n-k)-uniform hypergraph state.

关键词： multicontrol unitary operation cross phase modulation c-path gate merging gate hypergraph state

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Optical parity gate and a wide range of entangled states generation

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Science China(Physics,Mechanics & Astronomy) 2015年第4期58卷 30-39页

作者： lin qing College of Information Science and Engineering Huaqiao University (Xiamen)

Generating entangled states efficiently is a hot topic in the area of quantum information *** the approach presented in this paper,a general parity gate could be realized and a wide range of entangled states,including GHZ state,W state,Dicke state,arbitrary graph state and locally maximally entanglable states,can be generated *** generation of GHZ state,W state,and Dicke state is probabilistic but heralded and the total success probability is *** addition,the arbitrary graph state and locally maximally entanglable states generation is deterministic,flexible,and highly ***,with the"simultaneous"generation pattern,the complexity of the graph state generation and locally maximally entanglable states generation could be reduced greatly,providing a more efficient and feasible way to generate the entangled states.

关键词： parity gate entangled states cross phase modulation c-path gate quantum information

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Covalent triazine-based frameworks confining cobalt single atoms for photocatalytic CO_(2) reduction and hydrogen production

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Journal of Materials Science & Technology 2022年第21期116卷 41-49页

作者： Guocheng huang Guiyun lin qing Niu Jinhong Bi ling Wu Department of Environmental Science and Engineering Fuzhou UniversityMinhouFujian 350108China State Key Laboratory of Photocatalysis on Energy and Environment Fuzhou UniversityMinhouFujian 350108China

Single-atom catalysts(SACs)have emerged as an advanced frontier in heterogeneous catalysis due to their potential to maximize the atomic ***,covalent triazine-based frameworks(CTFs)confining cobalt single atoms(Co-SA/CTF)photocatalysts have been synthesized and used for efficient CO_(2) reduction and hydrogen production under visible light *** resulted Co-SA/CTF demonstrate excellent photocatalytic activity,with the CO and H2 evolution rates reaching 1665.74μmol g^(−1) h^(−1) and 1293.18μmol g^(−1) h^(−1),respectively,far surpassing those of Co nanoparticles anchored CTF and pure CTF.A variety of instrumental analyses collectively indicated that Co single atoms sites served as the reaction center for activating the adsorbed CO_(2) molecules,which significantly improved the CO_(2) reduction ***,the introduction of Co single atoms could accelerate the separation/transfer of photogenerated charge carriers,thus boosting the photocatalytic *** study envisions a novel strategy for designing efficient photocatalysts for energy conversion and showcases the application of CTFs as attractive support for confining metal single atoms.

关键词： Covalent triazine-based frameworks Co single atoms Photocatalysis CO_(2)reduction Hydrogen evolution

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Synthesis,Characterization and Optical Properties of Poly(aryleneethynylene)s Containing BODIPY

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Chinese Journal of Polymer Science 2015年第8期33卷 1133-1139页

作者： Bin Liu Fang-lin huang Yong-feng Shi Zhi-qing Ni Ye lin 尹守春 Hangzhou Science and Technology Information Institute College of Material Chemistry and Chemical Engineering Hangzhou Normal University

Poly（aryleneethynylene）s containing BODIPY （4,4-difluoro-4-bora-3a,4a-diaza-s-indacene）（P 1） were prepared by palladium-catalyzed Sonogashira polymerization of 2,6-diiodo-functionalized BODIPY monomer （M1） with 1,4-diethynyl- 2,5-didodecyl-oxybenzene monomer （M2）. The structures of the monomer （M1） and copolymer （P1） were confirmed by IH-NMR and Fourier transform infrared （FTIR） spectroscopies; their optical properties were characterized by UV-Vis absorption, photoluminescence （PL） spectroscopy and the Z-scan technique. Both UV-Vis absorption and fluorescence emission of copolymer P1 red-shifted to longer wavelengths （λabs： from 502 nm to 542 nm; 2era： from 511 nm to 631 nm） relative to those of the monomer M1 due to the π-conjugation extension of the copolymer. The third-order nonlinear optical coefficient （x^（3）） of copolymer P1 was 1.94 × 10^-10 esu, which is almost two or three orders of magnitude greater than that of common conjugated polymers without BODPIY moiety.

关键词： BODIPY Conjugated polymers Nonlinear optics Synthesis.

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Fabrication of Ti/Al/Mg laminated composites by hot roll bonding and their microstructures and mechanical properties

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Chinese Journal of Aeronautics 2021年第8期34卷 192-201页

作者： Pengju WANG Zejun CHEN Hongtao huang Jianshu lin Boxin LI qing LIU College of Materials Science and Engineering Chongqing UniversityChongqing 400044China State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China

Ti/Al/Mg laminated composites were successfully fabricated by hot roll *** effects of the rolling reduction on the microstructural evolution and mechanical properties of the composites were *** results show that Ti/Al/Mg laminated sheets exhibit good interfacial *** rolling reduction has a significant effect on the deformation inhomogeneity through the thickness of the Al *** initial grains of the Al layer near the Ti/Al interface are fragmented into fine equiaxed grains,and the grains at the center and near the Al/Mg interface are *** R-cube shear texture of the Al layer forms near the Ti/Al interface and permeates into the center layer in the samples with greater rolling *** b-fiber rolling texture of the Al layer is observed near the Al/Mg interface and increases with the increase of rolling *** stress–strain curves indicate that the fracture appears first in the Mg *** the increasing rolling reduction,the ultimate tensile and yield strength values increase,and the elongation up to the Mg layer fracture decreases.

关键词： Al texture Hot roll bonding Interfacial microstructure Mechanical property Ti/Al/Mg laminated sheet

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A new PMSM speed modulation system with sliding mode based on active-disturbance-rejection control

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Journal of Central South University 2016年第6期23卷 1406-1415页

作者：荣智林黄庆 College of Electronics and Information Engineering Tongji University Shanghai 201804 China Zhuzhou CSR Times Electric Co. Ltd. Zhuzhou 412001 China

A sliding mode and active disturbance rejection control(SM-ADRC)was employed to regulate the speed of a permanent magnet synchronous motor(PMSM).The major advantages of the proposed control scheme are that it can maintain the original features of ADRC and make the parameters of ADRC transition *** proposed control scheme also ensures speed control accuracy and improves the robustness and anti-load disturbance ability of the ***,through the analysis of a d-axis current output equation,a novel current-loop SM-ADRC is presented to improve the system’s dynamic performance and inner ability of anti-load *** of a simulation and experiments show that the improved sliding-mode ADRC system has the advantages of fast response,small overshoot,small steady-state error,wide speed range and high control *** shows that the system has strong anti-interference ability to reduce the influence of variations in rotational inertia,load and internal parameters.

关键词： disturbance rejection auto tuning permanent magnet synchronous motor speed modulation system

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Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： lin-Yan huang Yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-Wei Pu Xin-Yian Guo Jian-Gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

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不同Child-Pugh分级肝硬化患者血小板参数与凝血指标的变化研究

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中国卫生检验杂志 2018年第6期28卷 686-688页

作者：林秀清黄智铭金瑞放黄尔炯陈新吴伟温州医科大学附属第一医院消化内科浙江温州325000

目的探讨不同Child-Pugh分级肝硬化患者血小板参数与凝血指标的变化特征。方法选取肝硬化患者98例,根据Child-Pugh分级标准分为A级、B级、C级3组,选取健康志愿者35例作为对照组,检测各组血小板参数及凝血指标水平。结果不同Child-Pugh... 详细信息

目的探讨不同Child-Pugh分级肝硬化患者血小板参数与凝血指标的变化特征。方法选取肝硬化患者98例,根据Child-Pugh分级标准分为A级、B级、C级3组,选取健康志愿者35例作为对照组,检测各组血小板参数及凝血指标水平。结果不同Child-Pugh分级肝硬化患者的PLT、PCT均低于对照组,PDW、MPV均高于对照组;PLT、PCT随着Child-Pugh分级水平的升高而降低,PDW、MPV随着Child-Pugh分级水平的升高而升高,上述差异均有统计学意义(P<0.05)。不同Child-Pugh分级肝硬化患者的PT、APTT、TT均比对照组延长,Fib均比对照组降低;PT、APTT、TT随着Child-Pugh分级水平的升高而延长,Fib随着Child-Pugh分级水平的升高而降低,上述差异均有统计学意义(P<0.05)。结论肝硬化患者均有血小板参数及凝血指标的异常改变,且与肝功能Child-Pugh分级密切相关。

关键词：肝硬化 Child-Pugh分级血小板凝血指标

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