检索结果-南通市图书馆

Efficient Gene Transfer Mediated by HIV-1-based Defective Lentivector and Inhibition of HIV-1 Replication

中国病毒学 2007年第4期22卷 266-279页

作者： ling-bing ZENG lin-bai ye Yuanan LU Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Jingzhou 434000 China Retrovirology Research Laboratory Pacific Biosciences Research Center University of Hawaii at Manoa Honolulu Hawaii 96822 USA College of Life Sciences Wuhan University Wuhan 430072 China

Lentiviral vectors have drawn considerable attention recently and show great promise to become important delivery vehicles for future gene transfer manipulation. In the present study we have optimized a protocol for preparation of human immunodeficiency virus type-1 (HIV-1)-based defective lentiviral vectors (DLV) and characterized these vectors in terms of their transduction of different cells. Transient co-transfection of 293T packaging cells with DNA plasmids encoding lentiviral vector constituents resulted in production of high-titer DLV (0.5-1.2 × 107IU/mL), which can be further concentrated over 100-fold through a single step ultracentrifugation. These vectors were capable of transducing a variety of cells from both primate and non-primate sources and high transduction efficiency was achieved using concentrated vectors. Assessment of potential generation of RCV revealed no detection of infection by infectious particles in DLV-transduced CEM, SupT-1 and MT-2 cells. Long-term culture of transduced cells showed a stable expression of transgenes without apparent alteration in cellular morphology and growth kinetics. Vector mobilization to untransduced cells mediated by wild-type HIV-1 infection was confirmed in this test. Challenge of transduced human T-lymphocytes with wild-type HIV-1 showed these cells are totally resistant to the viral infection. Considering the effective gene transfer and stable gene expression, safety and anti-HIV activity, these DLV vectors warrant further exploration for their potential use as a gene transfer vehicle in the development of gene therapy protocols.

关键词：基因转化艾滋病病毒-1 抑制作用辅助受体艾滋病

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Activation of NF-kB by the Full-length Nucleocapsid Protein of the SARS Coronavirus

引用

Acta Biochimica et Biophysica Sinica 2005年第9期37卷 607-612页

作者： Qing-Jiao LIAO lin-bai ye Khalid Amine TIMANI Ying-Chun ZENG Ying-Long SHE Li ye Zheng-Hui WU State Key Laboratory of Virology,College of Life Science,Wuhan University,Wuhan 430072,China State Key Laboratory of Virology College of Life Science Wuhan University Wuhan 430072 China.

The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agentfor the worldwide outbreak of SARS in *** mechanism by which SARS-CoV causes atypical pneu-monia remains *** nuclear factor kappa B (NF-kB) is a key transcription factor that activatesnumerous genes involved in cellular immune response and *** studies have shown that NF-kB plays an important role in the pathogenesis of lung *** this study,we investigated the possibleregulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-kB by luciferase *** results showed that the SARS-CoV N protein can significantly activate NF-kB only in Vero *** are susceptible to SARS-CoV infection,but not in Vero or HeLa *** suggests that NF-kBactivation is ***,NF-kB activation in Vero E6 cells expressing the N protein is *** experiments showed that there is more than one function domain in the N protein respon-sible for NF-kB *** data indicated the possible role of the N protein in the pathogenesis of SARS.

关键词： severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein NF-kB

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Isthmus Undivided Bilateral Nephrectomy in a Patient with Polycystic Horseshoe Kidney

引用

Chinese Medical Journal 2015年第5期128卷 698-699页

作者： Xiao-lin Yao bai-ye Jin Yi-Ming Wang Department of Urology The First Affiliated Hospital of Medical College Zhejiang University Hangzhou Zhejiang 310003 China

Horseshoe kidney is a type of renal fusion by the lower poles anomaly during *** kidney that occurs in adults is an autosomal dominant disease due to the genetic mutations leading to the expression of the proteins pol... 详细信息

关键词： Abnormalities Nephrectomy Polycystic Kidney Diseases

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigations of Water Flow Behaviors Induced by Local Temperature Variations through a Single Rough Fracture for the Enhanced Geothermal Systems

引用

Journal of Thermal Science 2024年第3期33卷 1055-1064页

作者： LI Jiawei ye lin Department of Energy and Power Engineering Tsinghua UniversityBeijing100084China School of Power and Energy Northwestern Polytechnical UniversityXi’an710072China

In recent years,Enhanced Geothermal System(EGS)technologies have been applied to the geothermal resources production in the Hot Dry Rock(HDR).The core of EGS technologies is to adopt hydraulic fracturing in the reservoir to create a connected network of discrete fractures with the consideration of water as a working fluid for hydraulic fracturing and heat *** paper investigates the characteristics of water flow behaviors through a single rough fracture under different temperature and pressure conditions.A single fracture model with rough fracture surfaces is constructed and then characterized,and influences of the anisotropic factor on the average tortuosity and frictional resistance coefficient of water flow through a single fracture with rough surfaces have been compared and *** consideration of other impacting factors(temperature,pressure,fracture roughness),the impact of mass flow rate has also been *** simulation results present that changes of average tortuosity for water flow through a single rough facture are mainly affected by *** can be observed that higher temperature leads to larger average tortuosity but the frictional resistance coefficient shows an opposite *** for pressure conditions,it is found that effects of pressure on average tortuosity and frictional resistance coefficient is very small,which can be neglected under high pressure ***,the average tortuosity shows a progressively linear relationship with the mass flow *** the contrary,the frictional resistance coefficient has a negative relationship with the mass flow *** is revealed that when the mass flow rate reaches a critical point,the influences of temperature on the frictional resistance coefficient will be *** of single rough fractures with different anisotropic factors show that values of average tortuosity and frictional resistance coefficient have positive relationships with the increase

关键词： single rough fracture tortuosity frictional resistance enhanced geothermal system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Heterogeneous body compositions and all-cause mortality in acute coronary syndrome patients:a ten-year retrospective cohort study

引用

Journal of Geriatric Cardiology 2024年第5期21卷 534-541页

作者： Guang-Zhi LIAO lin bai Yu-Yang ye Xue-Feng CHEN Xin-Ru HU Yong PENG Department of Cardiology West China HospitalSichuan UniversityChengduSichuanChina

BACKGROUND The association of different body components,including lean mass and body fat,with the risk of death in acute coronary syndrome(ACS)patients are *** We enrolled adults diagnosed with ACS at our center between January 2011 and December 2012 and obtained fol-low-up outcomes via telephone *** used restricted cubic splines(RCS)with the Cox proportional hazards model to analyze the associations between body mass index(BMI),predicted lean mass index(LMI),predicted body fat percentage(BF),and the value of LMI/BF with 10-year *** also examined the secondary outcome of death during *** During the maximum 10-year follow-up of 1398 patients,331 deaths(23.6%)occurred,and a U-shaped relationship was found between BMI and death risk(P_(nonlinearity)=0.03).After adjusting for age and history of diabetes,the overweight group(24≤BMIlinear relationship with a 10-year death risk(P_(nonlinearity)=0.24 and P_(nonlinearity)=0.38,respectively),while an increase in BF was associ-ated with increased mortality(P_(nonlinearity)=0.64).During hospitalization,31 deaths(2.2%)were recorded,and the associations of the indicators with in-hospital mortality were consistent with the long-term outcome *** Our study provides new insight into the“obesity paradox”in ACS patients,highlighting the importance of considering body composition *** LMI and BF may serve as useful tools for assessing nutritional status and predicting the prognosis of ACS,based on their linear associations with all-cause mortality.

关键词： patients mortality coronary

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reliability analysis of multimodal scene matching based on correlation peaks

引用

CHINESE JOURNAL OF AERONAUTICS 2024年第9期37卷 433-447页

作者： Yu, Zhirui Zhu, bai Han, Zhiqiang ye, Yuanxin Southwest Jiaotong Univ Fac Geosci & Engn Chengdu 611756 Peoples R China

The navigation system plays a pivotal role in guiding aircraft along designated routes, ensuring precise and punctual arrival at destinations. The integration of scene matching with an inertial navigation system enhances the capability of providing a dependable guarantee for successful accomplishment of flight missions. Nonetheless, assuring reliability in scene matching encounters significant challenges in areas characterized by repetitive or weak textures. To tackle these challenges, we propose a novel method to assess the reliability of scene matching based on the distinctive characteristics of correlation peaks. The proposed method leverages the fact that the similarity of the optimal matching result is significantly higher than that of the surrounding area, and three novel indicators (e.g., relative height, slope of a correlation peak, and ratio of a sub peak to the main peak) are determined to conjointly evaluate the reliability of scene matching. The proposed method entails matching a real-time image with a reference image to generate a correlation surface. A correlation peak is then obtained by extracting the portion of the correlation surface exhibiting a significant gradient. Additionally, the matching reliability is determined by considering the relative height, slope, and ratio of the peak collectively. Exhaustive experimental results with two sets of data demonstrate that the proposed method significantly outperforms traditional approaches in terms of precision, recall, and F1-score. These experiments also establish the efficacy of the proposed method in achieving reliable matching in challenging environments characterized by repetitive and weak textures. This enhancement holds the potential to significantly elevate scene-matching-based navigation. (c) 2024 Production and hosting by Elsevier Ltd. on behalf of Chinese Society of Aeronautics and Astronautics. This is an open access article under the CC BY-NC-ND license.

关键词： Unmanned aerial vehicle Scene matching navigation Multimodal scene matching Correlation peak Reliability analysis SAR REGISTRATION IMAGES FRAMEWORK

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Boundary Behaviors for a Continuous-state Nonlinear Neveu’s Branching Process

引用

Acta Mathematica Sinica,English Series 2024年第8期40卷 2005-2016页

作者： lin Yu bai Xu YANG School of Mathematics and Information Science North Minzu UniversityYinchuan 750021P.R.China

By generalizing a criterion of Mufa Chen for Markov jump processes,we establish the necessary and sufficient conditions for the extinction,explosion and coming down from infinity of a continuous-state nonlinear Neveu... 详细信息

关键词： Continuous-state branching process nonlinear branching Neveu’s branching extinction explosion coming down from infinity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Soluble interleukin-6 receptor is elevated during influenza A virus infection and mediates the IL-6 and IL-32 inflammatory cytokine burst

引用

Cellular & Molecular Immunology 2015年第5期12卷 633-644页

作者： Jun Wang Qing Wang Tao Han Yong-Kui Li Sheng-Li Zhu Fang Ao Jian Feng Ming-Zhen Jing Li Wang lin-bai ye Ying Zhu The State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan China

Influenza A virus （IAV） infection is a major worldwide public health problem. However, the factors involved in mediating the inflammatory response to this infection and their relationships remain poorly understood. Here, we show that IAV infection stimulates the expression of the soluble IL-6 receptor （slL-6R）, a multifunctional protein involved in IL-6 signaling. Interestingly, slL-6R expression upregulated the levels of its own ligand, IL-6 and those of the pro-inflammatory cytokine IL-32. shRNA-mediated knockdown of slL-6R suppressed I L-6 and IL-32, indicating that this regulation is dependent on slL-6R during IAV infection. Furthermore, our results demonstrate that IL-32 participates in a negative feedback loop that inhibits slL-6R while upregulating IL-6 expression during IAV infection. Therefore, we show that slL-6R is a critical cellular factor involved in the acute inflammatory response to viral infection.

关键词： IL-32 IL-6 inflammation network influenza A virus soluble IL-6 receptor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Social Robot Detection Method with Improved Graph Neural Networks

引用

Computers, Materials & Continua 2024年第2期78卷 1773-1795页

作者： Zhenhua Yu Liangxue bai Ou ye Xuya Cong Institute of Systems Security and Control College of Computer Science and TechnologyXi’an University of Science and TechnologyXi’an710054China

Social robot accounts controlled by artificial intelligence or humans are active in social networks,bringing negative impacts to network security and social *** social robot detection methods based on graph neural networks suffer from the problem of many social network nodes and complex relationships,which makes it difficult to accurately describe the difference between the topological relations of nodes,resulting in low detection accuracy of social *** paper proposes a social robot detection method with the use of an improved neural ***,social relationship subgraphs are constructed by leveraging the user’s social network to disentangle intricate social relationships ***,a linear modulated graph attention residual network model is devised to extract the node and network topology features of the social relation subgraph,thereby generating comprehensive social relation subgraph features,and the feature-wise linear modulation module of the model can better learn the differences between the ***,user text content and behavioral gene sequences are extracted to construct social behavioral features combined with the social relationship subgraph ***,social robots can be more accurately identified by combining user behavioral and relationship *** carrying out experimental studies based on the publicly available datasets TwiBot-20 and Cresci-15,the suggested method’s detection accuracies can achieve 86.73%and 97.86%,*** with the existing mainstream approaches,the accuracy of the proposed method is 2.2%and 1.35%higher on the two *** results show that the method proposed in this paper can effectively detect social robots and maintain a healthy ecological environment of social networks.

关键词： Social robot detection social relationship subgraph graph attention network feature linear modulation behavioral gene sequences

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluating CMIP6 models in simulating the North Pacific decadal variability in sea surface salinity

引用

Science China Earth Sciences 2024年第8期67卷 2661-2680页

作者： Jian CHEN Hailong LIU Pengfei lin Wenrong bai Chengzu bai Lu WANG Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China State Key Laboratory of Geo-Information Engineering Xi’an 710054China Beijing Municipal Climate Center Beijing Meteorological BureauBeijing 100089China Space System Department Equipment Department Beijing 100096China

The North Pacific sea surface salinity(SSS)decadal variability(NPSDV)and its potential forcing were evaluated from 25 coupled models of the Coupled Model Intercomparison Project phase 6(CMIP6)considering the prospects for decadal climate *** results indicated that the CMIP6 models generally reproduced the spatial patterns of *** large standard deviation of the SSS anomaly over the strong current regions,such as the Kuroshio-Oyashio Extension(KOE),North Pacific Current(NPC),California Current System(CCS),and Alaskan Coastal Current(ACC),is reflected in the two leading modes of NPSDV:a dipole with out-of-phase loadings in the KOE-NPC versus CCS-ACC and a monopole with positive loading over the *** order of modes is sensitive to individual models that exhibit discrepancies,especially in temporal phases and power *** autoregressive model of order-1 was used to reconstruct the NPSDV with several forcing *** generally weaker influence of forcings in an autoregressive model of order-1 is partly related to the overestimated response time of NPSDV relative to *** NPSDV variances originate from the persistence of SSS anomalies,but the dominant forcing factors are diverse among *** model diversity for the NPSDV simulation mainly arises from the influence of the tropical El Ni?o-Southern Oscillation through teleconnection on the North Pacific Oscillation or Aleutian Low with timescale ***,models that can reproduce the NPSDV well are not dependent on those with larger impacts from the North Pacific oceanic processes.

关键词： North Pacific Sea surface salinity Decadal variability CMIP6 Leading mode

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：